Genotoxicity and heating Performance of VxFe3-xO4 nanoparticles in Health applications

• Autores: Sanz Sagué, Beatriz; Sáenz Hernández, Amaia; Moreno Maldonado, Ana C.; Fuentes García, Jesús A.; Nuñez, Jorge Martín; Zegura, Bojana; Stern, Alja; Kolosa, Katja; Rozman, Iza; Torres Molina, Teobaldo Enrique; Goya, Gerardo F.
• Año de publicación: 2024
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: Magnetica nanoparticles; Vanadium ferrite; Cytotoxicity; Genotoxicity
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

The applications of magnetic nanoparticles (MNPs) as biocatalysts in different biomedical areas have been evolved very recently. One of the main challenges in this field is to design affective MNPs surfaces with catalytically active atomic centres, while producing minimal toxicological side effects on the hosting cell or tissues. MNPs of vanadium spinel ferrite (VFe2O4) are a promising material for mimicking the action of natural enzymes in degrading harmful substrates due to the presence of active V5+ centres. However, the toxicity of this material has not been yet studied in detail enough to grant biomedical safety. In this work, we have extensively measured the structural, compositional, and magnetic properties of a series of VxFe3-xO4 spinel ferrite MNPs to assess the surface composition and oxidation state of V atoms, and also performed systematic and extensive in vitro cytotoxicity and genotoxicity testing required to assess their safety in potential clinical applications. We could establish the presence of V5+ at the particle surface even in water-based colloidal samples at pH 7, as well as different amounts of V2+ and V3+ substitution at the A and B sites of the spinel structure. All samples showed large heating efficiency with Specific Loss Power values up to 400 W/g (H0 = 30 kA/m; f = 700 kHz). Samples analysed for safety in human hepatocellular carcinoma (HepG2) cell line with up to 24h of exposure showed that these MNPs did not induce major genomic abnormalities such as micronuclei, nuclear buds, or nucleoplasmic bridges (MNIs, NBUDs, and NPBs), nor did they cause DNA double-strand breaks (DSBs) or aneugenic effects—types of damage considered most harmful to cellular genetic material. The present study is an essential step towards the use of these type of nanomaterials in any biomedical or clinical application.
Fil: Sanz Sagué, Beatriz. Universidad de Zaragoza. Facultad de Ciencias; España
Fil: Sáenz Hernández, Amaia. Universidad de Zaragoza. Facultad de Ciencias; España
Fil: Moreno Maldonado, Ana C.. Universidad de Zaragoza. Facultad de Ciencias; España
Fil: Fuentes García, Jesús A.. Universidad de Zaragoza. Facultad de Ciencias; España
Fil: Nuñez, Jorge Martín. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Unidad Ejecutora Instituto de Nanociencia y Nanotecnologia. Unidad Ejecutora Instituto de Nanociencia y Nanotecnologia - Nodo Bariloche | Comision Nacional de Energia Atomica. Unidad Ejecutora Instituto de Nanociencia y Nanotecnologia. Unidad Ejecutora Instituto de Nanociencia y Nanotecnologia - Nodo Bariloche.; Argentina. Universidad de Zaragoza. Facultad de Ciencias; España
Fil: Zegura, Bojana. University of Ljubljana; Eslovenia
Fil: Stern, Alja. University of Ljubljana; Eslovenia
Fil: Kolosa, Katja. No especifíca;
Fil: Rozman, Iza. University of Ljubljana; Eslovenia
Fil: Torres Molina, Teobaldo Enrique. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Unidad Ejecutora Instituto de Nanociencia y Nanotecnologia. Unidad Ejecutora Instituto de Nanociencia y Nanotecnologia - Nodo Bariloche | Comision Nacional de Energia Atomica. Unidad Ejecutora Instituto de Nanociencia y Nanotecnologia. Unidad Ejecutora Instituto de Nanociencia y Nanotecnologia - Nodo Bariloche.; Argentina. Universidad de Zaragoza. Facultad de Ciencias; España
Fil: Goya, Gerardo F.. Universidad de Zaragoza. Facultad de Ciencias; España