Epitope mapping of pathogenic leptospira LipL32

Autores
Lottersberger, Javier; Guerrero, Sergio Adrian; Tonarelli, Georgina Guadalupe; Frank, R.; Tarabla, Hector Dante; Vanasco, Norma Bibiana
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims: To identify LipL32 epitopes and to evaluate their capability to recognizespecific antibodies using ELISA.Methods and Results: Epitope mapping by means of a library of overlappingpeptide fragments prepared by simultaneous and parallel solid phase peptidesynthesis on derivatized cellulose membranes (SPOT synthesis) was carried out.Eighty-seven overlapping decapentapeptides corresponding to the completesequence of LipL32 were synthesized. According to spot-image intensities, themost reactive sequences were localized in regions 151?177 (sequenceAAKAKPVQKLDDDDDGDDTYKEERHNK) and 181?204 (sequence LTRIKIPNPPKSFDDLKNIDTKKL).Two peptides (P1 and P2) corresponding tothese sequences were synthesized, and their reactivity evaluated using ELISAtest.Conclusions: Epitope identification and analysis suggested the existence of twoantigenic regions within LipL32. These LipL32 reactive regions were highlyconserved among antigenically variants of Leptospira spp. isolates. Peptidescontaining these regions (P1 and P2) showed a good capability for anti-leptospiralantibody recognition.Significance and Impact of the Study: This finding could have potential relevancenot only for serodiagnosis but also as a starting point for the characterizationof targets for vaccine design.
Fil: Lottersberger, Javier. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Guerrero, Sergio Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Tonarelli, Georgina Guadalupe. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Frank, R.. Helmholtz Centre for Environmental Research; Alemania
Fil: Tarabla, Hector Dante. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Vanasco, Norma Bibiana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorios e Instituto de Salud "Dr. C. G. Malbran". Instituto Nacional de Enfermedades Respiratorias; Argentina
Materia
EPITOPE
LEPTOSPIRA
LIPLP32
MAPPING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/84710
Acceder
id CONICETDig_8fd0aa04b65e67d2661f1100bf039afe
oai_identifier_str oai:ri.conicet.gov.ar:11336/84710
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Epitope mapping of pathogenic leptospira LipL32Lottersberger, JavierGuerrero, Sergio AdrianTonarelli, Georgina GuadalupeFrank, R.Tarabla, Hector DanteVanasco, Norma BibianaEPITOPELEPTOSPIRALIPLP32MAPPINGhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Aims: To identify LipL32 epitopes and to evaluate their capability to recognizespecific antibodies using ELISA.Methods and Results: Epitope mapping by means of a library of overlappingpeptide fragments prepared by simultaneous and parallel solid phase peptidesynthesis on derivatized cellulose membranes (SPOT synthesis) was carried out.Eighty-seven overlapping decapentapeptides corresponding to the completesequence of LipL32 were synthesized. According to spot-image intensities, themost reactive sequences were localized in regions 151?177 (sequenceAAKAKPVQKLDDDDDGDDTYKEERHNK) and 181?204 (sequence LTRIKIPNPPKSFDDLKNIDTKKL).Two peptides (P1 and P2) corresponding tothese sequences were synthesized, and their reactivity evaluated using ELISAtest.Conclusions: Epitope identification and analysis suggested the existence of twoantigenic regions within LipL32. These LipL32 reactive regions were highlyconserved among antigenically variants of Leptospira spp. isolates. Peptidescontaining these regions (P1 and P2) showed a good capability for anti-leptospiralantibody recognition.Significance and Impact of the Study: This finding could have potential relevancenot only for serodiagnosis but also as a starting point for the characterizationof targets for vaccine design.Fil: Lottersberger, Javier. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Guerrero, Sergio Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Tonarelli, Georgina Guadalupe. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Frank, R.. Helmholtz Centre for Environmental Research; AlemaniaFil: Tarabla, Hector Dante. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; ArgentinaFil: Vanasco, Norma Bibiana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorios e Instituto de Salud "Dr. C. G. Malbran". Instituto Nacional de Enfermedades Respiratorias; ArgentinaWiley Blackwell Publishing, Inc2009info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/84710Lottersberger, Javier; Guerrero, Sergio Adrian; Tonarelli, Georgina Guadalupe; Frank, R.; Tarabla, Hector Dante; et al.; Epitope mapping of pathogenic leptospira LipL32; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 49; 5; 2009; 41-450266-8254CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1472-765X.2009.02723.x/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1472-765X.2009.02723.x/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:10Zoai:ri.conicet.gov.ar:11336/84710instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:10.507CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Epitope mapping of pathogenic leptospira LipL32
title Epitope mapping of pathogenic leptospira LipL32
spellingShingle Epitope mapping of pathogenic leptospira LipL32
Lottersberger, Javier
EPITOPE
LEPTOSPIRA
LIPLP32
MAPPING
title_short Epitope mapping of pathogenic leptospira LipL32
title_full Epitope mapping of pathogenic leptospira LipL32
title_fullStr Epitope mapping of pathogenic leptospira LipL32
title_full_unstemmed Epitope mapping of pathogenic leptospira LipL32
title_sort Epitope mapping of pathogenic leptospira LipL32
dc.creator.none.fl_str_mv Lottersberger, Javier
Guerrero, Sergio Adrian
Tonarelli, Georgina Guadalupe
Frank, R.
Tarabla, Hector Dante
Vanasco, Norma Bibiana
author Lottersberger, Javier
author_facet Lottersberger, Javier
Guerrero, Sergio Adrian
Tonarelli, Georgina Guadalupe
Frank, R.
Tarabla, Hector Dante
Vanasco, Norma Bibiana
author_role author
author2 Guerrero, Sergio Adrian
Tonarelli, Georgina Guadalupe
Frank, R.
Tarabla, Hector Dante
Vanasco, Norma Bibiana
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv EPITOPE
LEPTOSPIRA
LIPLP32
MAPPING
topic EPITOPE
LEPTOSPIRA
LIPLP32
MAPPING
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Aims: To identify LipL32 epitopes and to evaluate their capability to recognizespecific antibodies using ELISA.Methods and Results: Epitope mapping by means of a library of overlappingpeptide fragments prepared by simultaneous and parallel solid phase peptidesynthesis on derivatized cellulose membranes (SPOT synthesis) was carried out.Eighty-seven overlapping decapentapeptides corresponding to the completesequence of LipL32 were synthesized. According to spot-image intensities, themost reactive sequences were localized in regions 151?177 (sequenceAAKAKPVQKLDDDDDGDDTYKEERHNK) and 181?204 (sequence LTRIKIPNPPKSFDDLKNIDTKKL).Two peptides (P1 and P2) corresponding tothese sequences were synthesized, and their reactivity evaluated using ELISAtest.Conclusions: Epitope identification and analysis suggested the existence of twoantigenic regions within LipL32. These LipL32 reactive regions were highlyconserved among antigenically variants of Leptospira spp. isolates. Peptidescontaining these regions (P1 and P2) showed a good capability for anti-leptospiralantibody recognition.Significance and Impact of the Study: This finding could have potential relevancenot only for serodiagnosis but also as a starting point for the characterizationof targets for vaccine design.
Fil: Lottersberger, Javier. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Guerrero, Sergio Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Tonarelli, Georgina Guadalupe. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Frank, R.. Helmholtz Centre for Environmental Research; Alemania
Fil: Tarabla, Hector Dante. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Vanasco, Norma Bibiana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorios e Instituto de Salud "Dr. C. G. Malbran". Instituto Nacional de Enfermedades Respiratorias; Argentina
description Aims: To identify LipL32 epitopes and to evaluate their capability to recognizespecific antibodies using ELISA.Methods and Results: Epitope mapping by means of a library of overlappingpeptide fragments prepared by simultaneous and parallel solid phase peptidesynthesis on derivatized cellulose membranes (SPOT synthesis) was carried out.Eighty-seven overlapping decapentapeptides corresponding to the completesequence of LipL32 were synthesized. According to spot-image intensities, themost reactive sequences were localized in regions 151?177 (sequenceAAKAKPVQKLDDDDDGDDTYKEERHNK) and 181?204 (sequence LTRIKIPNPPKSFDDLKNIDTKKL).Two peptides (P1 and P2) corresponding tothese sequences were synthesized, and their reactivity evaluated using ELISAtest.Conclusions: Epitope identification and analysis suggested the existence of twoantigenic regions within LipL32. These LipL32 reactive regions were highlyconserved among antigenically variants of Leptospira spp. isolates. Peptidescontaining these regions (P1 and P2) showed a good capability for anti-leptospiralantibody recognition.Significance and Impact of the Study: This finding could have potential relevancenot only for serodiagnosis but also as a starting point for the characterizationof targets for vaccine design.
publishDate 2009
dc.date.none.fl_str_mv 2009
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/84710
Lottersberger, Javier; Guerrero, Sergio Adrian; Tonarelli, Georgina Guadalupe; Frank, R.; Tarabla, Hector Dante; et al.; Epitope mapping of pathogenic leptospira LipL32; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 49; 5; 2009; 41-45
0266-8254
CONICET Digital
CONICET
url http://hdl.handle.net/11336/84710
identifier_str_mv Lottersberger, Javier; Guerrero, Sergio Adrian; Tonarelli, Georgina Guadalupe; Frank, R.; Tarabla, Hector Dante; et al.; Epitope mapping of pathogenic leptospira LipL32; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 49; 5; 2009; 41-45
0266-8254
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1472-765X.2009.02723.x/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1472-765X.2009.02723.x/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.885934

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