Expression and characterization of HlyX hemolysin from Leptospira interrogans serovar Copenhageni: Potentiation of hemolytic activity by LipL32

Autores
Hauk, Pricila; Negrotto, Soledad; Romero, Eliete Caló; Vasconcellos, Sílvio Arruda; Genovez, Margareth Élide; Ward, Richard John; Schattner, Mirta Ana; Gomez, Ricardo Martin; Ho, Paulo Lee
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The HlyX, a putative hemolysin identified from the Leptospira genomes, was cloned, expressed in Escherichia coli, purified, and its hemolytic activity was confirmed. Mouse polyclonal antiserum against the recombinant HlyX recognized HlyX-related antigens in a panel of Leptospira species extracts and it was also able to abolish the hemolytic activity of HlyX. A mixture of HlyX and LipL32, a known hemolysin from Leptospira, induced hemolysis in a synergistic way that was fully inhibited by antiserum against either protein. Moreover, sera from patients with leptospirosis also recognized the recombinant HlyX, showing that it is presented to the host immune system during Leptospira infection. © 2005 Elsevier Inc. All rights reserved.
Fil: Hauk, Pricila. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil. Universidade de Sao Paulo; Brasil
Fil: Negrotto, Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Romero, Eliete Caló. Instituto Adolfo Lutz; Brasil
Fil: Vasconcellos, Sílvio Arruda. Universidade de Sao Paulo; Brasil
Fil: Genovez, Margareth Élide. Centro de Pesquisa e Desenvolvimento de Sanidade Animal; Brasil
Fil: Ward, Richard John. Universidade de Sao Paulo; Brasil
Fil: Schattner, Mirta Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Gomez, Ricardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Ho, Paulo Lee. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil. Universidade de Sao Paulo; Brasil
Materia
Hemolysin
Hlyx
Leptospira Interrogans Serovar Copenhageni
Leptospirosis
Lipl32
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/62676
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/62676
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Expression and characterization of HlyX hemolysin from Leptospira interrogans serovar Copenhageni: Potentiation of hemolytic activity by LipL32Hauk, PricilaNegrotto, SoledadRomero, Eliete CalóVasconcellos, Sílvio ArrudaGenovez, Margareth ÉlideWard, Richard JohnSchattner, Mirta AnaGomez, Ricardo MartinHo, Paulo LeeHemolysinHlyxLeptospira Interrogans Serovar CopenhageniLeptospirosisLipl32https://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3The HlyX, a putative hemolysin identified from the Leptospira genomes, was cloned, expressed in Escherichia coli, purified, and its hemolytic activity was confirmed. Mouse polyclonal antiserum against the recombinant HlyX recognized HlyX-related antigens in a panel of Leptospira species extracts and it was also able to abolish the hemolytic activity of HlyX. A mixture of HlyX and LipL32, a known hemolysin from Leptospira, induced hemolysis in a synergistic way that was fully inhibited by antiserum against either protein. Moreover, sera from patients with leptospirosis also recognized the recombinant HlyX, showing that it is presented to the host immune system during Leptospira infection. © 2005 Elsevier Inc. All rights reserved.Fil: Hauk, Pricila. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil. Universidade de Sao Paulo; BrasilFil: Negrotto, Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Romero, Eliete Caló. Instituto Adolfo Lutz; BrasilFil: Vasconcellos, Sílvio Arruda. Universidade de Sao Paulo; BrasilFil: Genovez, Margareth Élide. Centro de Pesquisa e Desenvolvimento de Sanidade Animal; BrasilFil: Ward, Richard John. Universidade de Sao Paulo; BrasilFil: Schattner, Mirta Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Gomez, Ricardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Ho, Paulo Lee. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil. Universidade de Sao Paulo; BrasilAcademic Press Inc Elsevier Science2005-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/62676Hauk, Pricila; Negrotto, Soledad; Romero, Eliete Caló; Vasconcellos, Sílvio Arruda; Genovez, Margareth Élide; et al.; Expression and characterization of HlyX hemolysin from Leptospira interrogans serovar Copenhageni: Potentiation of hemolytic activity by LipL32; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 333; 4; 8-2005; 1341-13470006-291XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbrc.2005.06.045info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0006291X05012623info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:15:47Zoai:ri.conicet.gov.ar:11336/62676instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:15:47.335CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expression and characterization of HlyX hemolysin from Leptospira interrogans serovar Copenhageni: Potentiation of hemolytic activity by LipL32
title Expression and characterization of HlyX hemolysin from Leptospira interrogans serovar Copenhageni: Potentiation of hemolytic activity by LipL32
spellingShingle Expression and characterization of HlyX hemolysin from Leptospira interrogans serovar Copenhageni: Potentiation of hemolytic activity by LipL32
Hauk, Pricila
Hemolysin
Hlyx
Leptospira Interrogans Serovar Copenhageni
Leptospirosis
Lipl32
title_short Expression and characterization of HlyX hemolysin from Leptospira interrogans serovar Copenhageni: Potentiation of hemolytic activity by LipL32
title_full Expression and characterization of HlyX hemolysin from Leptospira interrogans serovar Copenhageni: Potentiation of hemolytic activity by LipL32
title_fullStr Expression and characterization of HlyX hemolysin from Leptospira interrogans serovar Copenhageni: Potentiation of hemolytic activity by LipL32
title_full_unstemmed Expression and characterization of HlyX hemolysin from Leptospira interrogans serovar Copenhageni: Potentiation of hemolytic activity by LipL32
title_sort Expression and characterization of HlyX hemolysin from Leptospira interrogans serovar Copenhageni: Potentiation of hemolytic activity by LipL32
dc.creator.none.fl_str_mv Hauk, Pricila
Negrotto, Soledad
Romero, Eliete Caló
Vasconcellos, Sílvio Arruda
Genovez, Margareth Élide
Ward, Richard John
Schattner, Mirta Ana
Gomez, Ricardo Martin
Ho, Paulo Lee
author Hauk, Pricila
author_facet Hauk, Pricila
Negrotto, Soledad
Romero, Eliete Caló
Vasconcellos, Sílvio Arruda
Genovez, Margareth Élide
Ward, Richard John
Schattner, Mirta Ana
Gomez, Ricardo Martin
Ho, Paulo Lee
author_role author
author2 Negrotto, Soledad
Romero, Eliete Caló
Vasconcellos, Sílvio Arruda
Genovez, Margareth Élide
Ward, Richard John
Schattner, Mirta Ana
Gomez, Ricardo Martin
Ho, Paulo Lee
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Hemolysin
Hlyx
Leptospira Interrogans Serovar Copenhageni
Leptospirosis
Lipl32
topic Hemolysin
Hlyx
Leptospira Interrogans Serovar Copenhageni
Leptospirosis
Lipl32
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The HlyX, a putative hemolysin identified from the Leptospira genomes, was cloned, expressed in Escherichia coli, purified, and its hemolytic activity was confirmed. Mouse polyclonal antiserum against the recombinant HlyX recognized HlyX-related antigens in a panel of Leptospira species extracts and it was also able to abolish the hemolytic activity of HlyX. A mixture of HlyX and LipL32, a known hemolysin from Leptospira, induced hemolysis in a synergistic way that was fully inhibited by antiserum against either protein. Moreover, sera from patients with leptospirosis also recognized the recombinant HlyX, showing that it is presented to the host immune system during Leptospira infection. © 2005 Elsevier Inc. All rights reserved.
Fil: Hauk, Pricila. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil. Universidade de Sao Paulo; Brasil
Fil: Negrotto, Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Romero, Eliete Caló. Instituto Adolfo Lutz; Brasil
Fil: Vasconcellos, Sílvio Arruda. Universidade de Sao Paulo; Brasil
Fil: Genovez, Margareth Élide. Centro de Pesquisa e Desenvolvimento de Sanidade Animal; Brasil
Fil: Ward, Richard John. Universidade de Sao Paulo; Brasil
Fil: Schattner, Mirta Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Gomez, Ricardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Ho, Paulo Lee. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil. Universidade de Sao Paulo; Brasil
description The HlyX, a putative hemolysin identified from the Leptospira genomes, was cloned, expressed in Escherichia coli, purified, and its hemolytic activity was confirmed. Mouse polyclonal antiserum against the recombinant HlyX recognized HlyX-related antigens in a panel of Leptospira species extracts and it was also able to abolish the hemolytic activity of HlyX. A mixture of HlyX and LipL32, a known hemolysin from Leptospira, induced hemolysis in a synergistic way that was fully inhibited by antiserum against either protein. Moreover, sera from patients with leptospirosis also recognized the recombinant HlyX, showing that it is presented to the host immune system during Leptospira infection. © 2005 Elsevier Inc. All rights reserved.
publishDate 2005
dc.date.none.fl_str_mv 2005-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/62676
Hauk, Pricila; Negrotto, Soledad; Romero, Eliete Caló; Vasconcellos, Sílvio Arruda; Genovez, Margareth Élide; et al.; Expression and characterization of HlyX hemolysin from Leptospira interrogans serovar Copenhageni: Potentiation of hemolytic activity by LipL32; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 333; 4; 8-2005; 1341-1347
0006-291X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/62676
identifier_str_mv Hauk, Pricila; Negrotto, Soledad; Romero, Eliete Caló; Vasconcellos, Sílvio Arruda; Genovez, Margareth Élide; et al.; Expression and characterization of HlyX hemolysin from Leptospira interrogans serovar Copenhageni: Potentiation of hemolytic activity by LipL32; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 333; 4; 8-2005; 1341-1347
0006-291X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbrc.2005.06.045
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0006291X05012623
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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