Characterizing monoclonal antibody epitopes by filtered gene fragment phage display

Autores
Di Niro, Roberto; Ferrara, Fortunato; Not, Tarcisio; Bradbury, Andrew; Chirdo, Fernando Gabriel; Marzari, Roberto; Sblattero, Daniele
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the present paper, we describe a novel approach to map monoclonal antibody epitopes, using three new monoclonal antibodies that recognize h-TG2 (human transglutaminase 2) as an example. The target gene was fragmented and cloned upstream of an antibiotic-resistance gene, in the vector pPAO2, to select for in-frame polypeptides. After removal of the antibiotic-resistance gene by Cre/Lox recombination, an antigen fragment phage display library was created and selected against specific monoclonal antibodies. Using the h-TG2 fragment library, we were able to identify epitopes. This technique can also be broadly applied to the study of protein-protein interactions.
Facultad de Ciencias Exactas
Materia
Bioquímica
Phage display
Epitope mapping
β-lactamase
Transglutaminase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/126484
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/126484
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Characterizing monoclonal antibody epitopes by filtered gene fragment phage displayDi Niro, RobertoFerrara, FortunatoNot, TarcisioBradbury, AndrewChirdo, Fernando GabrielMarzari, RobertoSblattero, DanieleBioquímicaPhage displayEpitope mappingβ-lactamaseTransglutaminaseIn the present paper, we describe a novel approach to map monoclonal antibody epitopes, using three new monoclonal antibodies that recognize h-TG2 (human transglutaminase 2) as an example. The target gene was fragmented and cloned upstream of an antibiotic-resistance gene, in the vector pPAO2, to select for in-frame polypeptides. After removal of the antibiotic-resistance gene by Cre/Lox recombination, an antigen fragment phage display library was created and selected against specific monoclonal antibodies. Using the h-TG2 fragment library, we were able to identify epitopes. This technique can also be broadly applied to the study of protein-protein interactions.Facultad de Ciencias Exactas2005-06-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf889-894http://sedici.unlp.edu.ar/handle/10915/126484enginfo:eu-repo/semantics/altIdentifier/issn/0264-6021info:eu-repo/semantics/altIdentifier/issn/1470-8728info:eu-repo/semantics/altIdentifier/doi/10.1042/bj20041983info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-11-12T10:54:37Zoai:sedici.unlp.edu.ar:10915/126484Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-11-12 10:54:38.084SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Characterizing monoclonal antibody epitopes by filtered gene fragment phage display
title Characterizing monoclonal antibody epitopes by filtered gene fragment phage display
spellingShingle Characterizing monoclonal antibody epitopes by filtered gene fragment phage display
Di Niro, Roberto
Bioquímica
Phage display
Epitope mapping
β-lactamase
Transglutaminase
title_short Characterizing monoclonal antibody epitopes by filtered gene fragment phage display
title_full Characterizing monoclonal antibody epitopes by filtered gene fragment phage display
title_fullStr Characterizing monoclonal antibody epitopes by filtered gene fragment phage display
title_full_unstemmed Characterizing monoclonal antibody epitopes by filtered gene fragment phage display
title_sort Characterizing monoclonal antibody epitopes by filtered gene fragment phage display
dc.creator.none.fl_str_mv Di Niro, Roberto
Ferrara, Fortunato
Not, Tarcisio
Bradbury, Andrew
Chirdo, Fernando Gabriel
Marzari, Roberto
Sblattero, Daniele
author Di Niro, Roberto
author_facet Di Niro, Roberto
Ferrara, Fortunato
Not, Tarcisio
Bradbury, Andrew
Chirdo, Fernando Gabriel
Marzari, Roberto
Sblattero, Daniele
author_role author
author2 Ferrara, Fortunato
Not, Tarcisio
Bradbury, Andrew
Chirdo, Fernando Gabriel
Marzari, Roberto
Sblattero, Daniele
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Bioquímica
Phage display
Epitope mapping
β-lactamase
Transglutaminase
topic Bioquímica
Phage display
Epitope mapping
β-lactamase
Transglutaminase
dc.description.none.fl_txt_mv In the present paper, we describe a novel approach to map monoclonal antibody epitopes, using three new monoclonal antibodies that recognize h-TG2 (human transglutaminase 2) as an example. The target gene was fragmented and cloned upstream of an antibiotic-resistance gene, in the vector pPAO2, to select for in-frame polypeptides. After removal of the antibiotic-resistance gene by Cre/Lox recombination, an antigen fragment phage display library was created and selected against specific monoclonal antibodies. Using the h-TG2 fragment library, we were able to identify epitopes. This technique can also be broadly applied to the study of protein-protein interactions.
Facultad de Ciencias Exactas
description In the present paper, we describe a novel approach to map monoclonal antibody epitopes, using three new monoclonal antibodies that recognize h-TG2 (human transglutaminase 2) as an example. The target gene was fragmented and cloned upstream of an antibiotic-resistance gene, in the vector pPAO2, to select for in-frame polypeptides. After removal of the antibiotic-resistance gene by Cre/Lox recombination, an antigen fragment phage display library was created and selected against specific monoclonal antibodies. Using the h-TG2 fragment library, we were able to identify epitopes. This technique can also be broadly applied to the study of protein-protein interactions.
publishDate 2005
dc.date.none.fl_str_mv 2005-06-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/126484
url http://sedici.unlp.edu.ar/handle/10915/126484
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0264-6021
info:eu-repo/semantics/altIdentifier/issn/1470-8728
info:eu-repo/semantics/altIdentifier/doi/10.1042/bj20041983
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
889-894
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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