Tracking Targeted Bimodal Nanovaccines: Immune Responses and Routing in Cells, Tissue, and Whole Organism

Autores
Cruz, Luis J.; Tacken, Paul J.; Zeelenberg, Ingrid S.; Srinivas, Mangala; Bonetto, Fernando Jose; Weigelin, Bettina; Eich, Christina; de Vries, I. Jolanda; Figdor, Carl G.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Dendritic cells (DCs) are the most potent antigen-presenting cells (APCs), involved in the induction of immunity and currently exploited for antitumor immunotherapies. An optimized noninvasive imaging modality capable of determining and quantifying DC-targeted nanoparticle (NP) trajectories could provide valuable information regarding therapeutic vaccine outcome. Here, targeted poly(d,l-lactide-co-glycolide) nanoparticles (PLGA NPs) recognizing DC receptors were equipped with superparamagnetic iron oxide particles (SPIO) or gold nanoparticles with fluorescently labeled antigen. The fluorescent label allowed for rapid analysis and quantification of DC-specific uptake of targeted PLGA NPs in comparison to uptake by other cells. Transmission electron microscopy (TEM) showed that a fraction of the encapsulated antigen reached the lysosomal compartment of DCs, where SPIO and gold were already partially released. However, part of the PLGA NPs localized within the cytoplasm, as confirmed by confocal microscopy. DCs targeted with NPs carrying SPIO or fluorescent antigen were detected within lymph nodes as early as 1 h after injection by magnetic resonance imaging (MRI). Despite the fact that targeting did not markedly affect PLGA NP biodistribution on organism and tissue level, it increased delivery of NPs to DCs residing in peripheral lymph nodes and resulted in enhanced T cell proliferation. In conclusion, two imaging agents within a single carrier allows tracking of targeted PLGA NPs at the subcellular, cellular, and organismal levels, thereby facilitating the rational design of in vivo targeted vaccination strategies.
Fil: Cruz, Luis J.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
Fil: Tacken, Paul J.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
Fil: Zeelenberg, Ingrid S.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
Fil: Srinivas, Mangala. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
Fil: Bonetto, Fernando Jose. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Física del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina
Fil: Weigelin, Bettina. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Cell Biology; Países Bajos
Fil: Eich, Christina. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
Fil: de Vries, I. Jolanda. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
Fil: Figdor, Carl G.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
Materia
Imaging
Nanocarriers
Biocompatible And Biodegradable Polymers
Nanomaterials
Contrast Agents
Fluorescence
Magnetic Resonance Imaging
Dendritic Cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4433
Acceder
id CONICETDig_8e6a8d8cfdecb89a930069a755a20d04
oai_identifier_str oai:ri.conicet.gov.ar:11336/4433
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Tracking Targeted Bimodal Nanovaccines: Immune Responses and Routing in Cells, Tissue, and Whole OrganismCruz, Luis J.Tacken, Paul J.Zeelenberg, Ingrid S.Srinivas, MangalaBonetto, Fernando JoseWeigelin, BettinaEich, Christinade Vries, I. JolandaFigdor, Carl G.ImagingNanocarriersBiocompatible And Biodegradable PolymersNanomaterialsContrast AgentsFluorescenceMagnetic Resonance ImagingDendritic Cellshttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Dendritic cells (DCs) are the most potent antigen-presenting cells (APCs), involved in the induction of immunity and currently exploited for antitumor immunotherapies. An optimized noninvasive imaging modality capable of determining and quantifying DC-targeted nanoparticle (NP) trajectories could provide valuable information regarding therapeutic vaccine outcome. Here, targeted poly(d,l-lactide-co-glycolide) nanoparticles (PLGA NPs) recognizing DC receptors were equipped with superparamagnetic iron oxide particles (SPIO) or gold nanoparticles with fluorescently labeled antigen. The fluorescent label allowed for rapid analysis and quantification of DC-specific uptake of targeted PLGA NPs in comparison to uptake by other cells. Transmission electron microscopy (TEM) showed that a fraction of the encapsulated antigen reached the lysosomal compartment of DCs, where SPIO and gold were already partially released. However, part of the PLGA NPs localized within the cytoplasm, as confirmed by confocal microscopy. DCs targeted with NPs carrying SPIO or fluorescent antigen were detected within lymph nodes as early as 1 h after injection by magnetic resonance imaging (MRI). Despite the fact that targeting did not markedly affect PLGA NP biodistribution on organism and tissue level, it increased delivery of NPs to DCs residing in peripheral lymph nodes and resulted in enhanced T cell proliferation. In conclusion, two imaging agents within a single carrier allows tracking of targeted PLGA NPs at the subcellular, cellular, and organismal levels, thereby facilitating the rational design of in vivo targeted vaccination strategies.Fil: Cruz, Luis J.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países BajosFil: Tacken, Paul J.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países BajosFil: Zeelenberg, Ingrid S.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países BajosFil: Srinivas, Mangala. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países BajosFil: Bonetto, Fernando Jose. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Física del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); ArgentinaFil: Weigelin, Bettina. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Cell Biology; Países BajosFil: Eich, Christina. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países BajosFil: de Vries, I. Jolanda. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países BajosFil: Figdor, Carl G.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países BajosAmerican Chemical Society2014-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4433Cruz, Luis J.; Tacken, Paul J.; Zeelenberg, Ingrid S.; Srinivas, Mangala; Bonetto, Fernando Jose; et al.; Tracking Targeted Bimodal Nanovaccines: Immune Responses and Routing in Cells, Tissue, and Whole Organism; American Chemical Society; Molecular Pharmaceutics; 11; 12; 10-2014; 4299–43131543-8384enginfo:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/mp400717rinfo:eu-repo/semantics/altIdentifier/doi/DOI:10.1021/mp400717rinfo:eu-repo/semantics/altIdentifier/issn/1543-8384info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:11Zoai:ri.conicet.gov.ar:11336/4433instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:11.601CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Tracking Targeted Bimodal Nanovaccines: Immune Responses and Routing in Cells, Tissue, and Whole Organism
title Tracking Targeted Bimodal Nanovaccines: Immune Responses and Routing in Cells, Tissue, and Whole Organism
spellingShingle Tracking Targeted Bimodal Nanovaccines: Immune Responses and Routing in Cells, Tissue, and Whole Organism
Cruz, Luis J.
Imaging
Nanocarriers
Biocompatible And Biodegradable Polymers
Nanomaterials
Contrast Agents
Fluorescence
Magnetic Resonance Imaging
Dendritic Cells
title_short Tracking Targeted Bimodal Nanovaccines: Immune Responses and Routing in Cells, Tissue, and Whole Organism
title_full Tracking Targeted Bimodal Nanovaccines: Immune Responses and Routing in Cells, Tissue, and Whole Organism
title_fullStr Tracking Targeted Bimodal Nanovaccines: Immune Responses and Routing in Cells, Tissue, and Whole Organism
title_full_unstemmed Tracking Targeted Bimodal Nanovaccines: Immune Responses and Routing in Cells, Tissue, and Whole Organism
title_sort Tracking Targeted Bimodal Nanovaccines: Immune Responses and Routing in Cells, Tissue, and Whole Organism
dc.creator.none.fl_str_mv Cruz, Luis J.
Tacken, Paul J.
Zeelenberg, Ingrid S.
Srinivas, Mangala
Bonetto, Fernando Jose
Weigelin, Bettina
Eich, Christina
de Vries, I. Jolanda
Figdor, Carl G.
author Cruz, Luis J.
author_facet Cruz, Luis J.
Tacken, Paul J.
Zeelenberg, Ingrid S.
Srinivas, Mangala
Bonetto, Fernando Jose
Weigelin, Bettina
Eich, Christina
de Vries, I. Jolanda
Figdor, Carl G.
author_role author
author2 Tacken, Paul J.
Zeelenberg, Ingrid S.
Srinivas, Mangala
Bonetto, Fernando Jose
Weigelin, Bettina
Eich, Christina
de Vries, I. Jolanda
Figdor, Carl G.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Imaging
Nanocarriers
Biocompatible And Biodegradable Polymers
Nanomaterials
Contrast Agents
Fluorescence
Magnetic Resonance Imaging
Dendritic Cells
topic Imaging
Nanocarriers
Biocompatible And Biodegradable Polymers
Nanomaterials
Contrast Agents
Fluorescence
Magnetic Resonance Imaging
Dendritic Cells
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Dendritic cells (DCs) are the most potent antigen-presenting cells (APCs), involved in the induction of immunity and currently exploited for antitumor immunotherapies. An optimized noninvasive imaging modality capable of determining and quantifying DC-targeted nanoparticle (NP) trajectories could provide valuable information regarding therapeutic vaccine outcome. Here, targeted poly(d,l-lactide-co-glycolide) nanoparticles (PLGA NPs) recognizing DC receptors were equipped with superparamagnetic iron oxide particles (SPIO) or gold nanoparticles with fluorescently labeled antigen. The fluorescent label allowed for rapid analysis and quantification of DC-specific uptake of targeted PLGA NPs in comparison to uptake by other cells. Transmission electron microscopy (TEM) showed that a fraction of the encapsulated antigen reached the lysosomal compartment of DCs, where SPIO and gold were already partially released. However, part of the PLGA NPs localized within the cytoplasm, as confirmed by confocal microscopy. DCs targeted with NPs carrying SPIO or fluorescent antigen were detected within lymph nodes as early as 1 h after injection by magnetic resonance imaging (MRI). Despite the fact that targeting did not markedly affect PLGA NP biodistribution on organism and tissue level, it increased delivery of NPs to DCs residing in peripheral lymph nodes and resulted in enhanced T cell proliferation. In conclusion, two imaging agents within a single carrier allows tracking of targeted PLGA NPs at the subcellular, cellular, and organismal levels, thereby facilitating the rational design of in vivo targeted vaccination strategies.
Fil: Cruz, Luis J.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
Fil: Tacken, Paul J.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
Fil: Zeelenberg, Ingrid S.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
Fil: Srinivas, Mangala. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
Fil: Bonetto, Fernando Jose. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Física del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina
Fil: Weigelin, Bettina. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Cell Biology; Países Bajos
Fil: Eich, Christina. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
Fil: de Vries, I. Jolanda. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
Fil: Figdor, Carl G.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
description Dendritic cells (DCs) are the most potent antigen-presenting cells (APCs), involved in the induction of immunity and currently exploited for antitumor immunotherapies. An optimized noninvasive imaging modality capable of determining and quantifying DC-targeted nanoparticle (NP) trajectories could provide valuable information regarding therapeutic vaccine outcome. Here, targeted poly(d,l-lactide-co-glycolide) nanoparticles (PLGA NPs) recognizing DC receptors were equipped with superparamagnetic iron oxide particles (SPIO) or gold nanoparticles with fluorescently labeled antigen. The fluorescent label allowed for rapid analysis and quantification of DC-specific uptake of targeted PLGA NPs in comparison to uptake by other cells. Transmission electron microscopy (TEM) showed that a fraction of the encapsulated antigen reached the lysosomal compartment of DCs, where SPIO and gold were already partially released. However, part of the PLGA NPs localized within the cytoplasm, as confirmed by confocal microscopy. DCs targeted with NPs carrying SPIO or fluorescent antigen were detected within lymph nodes as early as 1 h after injection by magnetic resonance imaging (MRI). Despite the fact that targeting did not markedly affect PLGA NP biodistribution on organism and tissue level, it increased delivery of NPs to DCs residing in peripheral lymph nodes and resulted in enhanced T cell proliferation. In conclusion, two imaging agents within a single carrier allows tracking of targeted PLGA NPs at the subcellular, cellular, and organismal levels, thereby facilitating the rational design of in vivo targeted vaccination strategies.
publishDate 2014
dc.date.none.fl_str_mv 2014-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4433
Cruz, Luis J.; Tacken, Paul J.; Zeelenberg, Ingrid S.; Srinivas, Mangala; Bonetto, Fernando Jose; et al.; Tracking Targeted Bimodal Nanovaccines: Immune Responses and Routing in Cells, Tissue, and Whole Organism; American Chemical Society; Molecular Pharmaceutics; 11; 12; 10-2014; 4299–4313
1543-8384
url http://hdl.handle.net/11336/4433
identifier_str_mv Cruz, Luis J.; Tacken, Paul J.; Zeelenberg, Ingrid S.; Srinivas, Mangala; Bonetto, Fernando Jose; et al.; Tracking Targeted Bimodal Nanovaccines: Immune Responses and Routing in Cells, Tissue, and Whole Organism; American Chemical Society; Molecular Pharmaceutics; 11; 12; 10-2014; 4299–4313
1543-8384
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/mp400717r
info:eu-repo/semantics/altIdentifier/doi/DOI:10.1021/mp400717r
info:eu-repo/semantics/altIdentifier/issn/1543-8384
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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