Tracking Targeted Bimodal Nanovaccines: Immune Responses and Routing in Cells, Tissue, and Whole Organism
- Autores
- Cruz, Luis J.; Tacken, Paul J.; Zeelenberg, Ingrid S.; Srinivas, Mangala; Bonetto, Fernando Jose; Weigelin, Bettina; Eich, Christina; de Vries, I. Jolanda; Figdor, Carl G.
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Dendritic cells (DCs) are the most potent antigen-presenting cells (APCs), involved in the induction of immunity and currently exploited for antitumor immunotherapies. An optimized noninvasive imaging modality capable of determining and quantifying DC-targeted nanoparticle (NP) trajectories could provide valuable information regarding therapeutic vaccine outcome. Here, targeted poly(d,l-lactide-co-glycolide) nanoparticles (PLGA NPs) recognizing DC receptors were equipped with superparamagnetic iron oxide particles (SPIO) or gold nanoparticles with fluorescently labeled antigen. The fluorescent label allowed for rapid analysis and quantification of DC-specific uptake of targeted PLGA NPs in comparison to uptake by other cells. Transmission electron microscopy (TEM) showed that a fraction of the encapsulated antigen reached the lysosomal compartment of DCs, where SPIO and gold were already partially released. However, part of the PLGA NPs localized within the cytoplasm, as confirmed by confocal microscopy. DCs targeted with NPs carrying SPIO or fluorescent antigen were detected within lymph nodes as early as 1 h after injection by magnetic resonance imaging (MRI). Despite the fact that targeting did not markedly affect PLGA NP biodistribution on organism and tissue level, it increased delivery of NPs to DCs residing in peripheral lymph nodes and resulted in enhanced T cell proliferation. In conclusion, two imaging agents within a single carrier allows tracking of targeted PLGA NPs at the subcellular, cellular, and organismal levels, thereby facilitating the rational design of in vivo targeted vaccination strategies.
Fil: Cruz, Luis J.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
Fil: Tacken, Paul J.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
Fil: Zeelenberg, Ingrid S.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
Fil: Srinivas, Mangala. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
Fil: Bonetto, Fernando Jose. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Física del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina
Fil: Weigelin, Bettina. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Cell Biology; Países Bajos
Fil: Eich, Christina. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
Fil: de Vries, I. Jolanda. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos
Fil: Figdor, Carl G.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos - Materia
-
Imaging
Nanocarriers
Biocompatible And Biodegradable Polymers
Nanomaterials
Contrast Agents
Fluorescence
Magnetic Resonance Imaging
Dendritic Cells - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/4433
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Tracking Targeted Bimodal Nanovaccines: Immune Responses and Routing in Cells, Tissue, and Whole OrganismCruz, Luis J.Tacken, Paul J.Zeelenberg, Ingrid S.Srinivas, MangalaBonetto, Fernando JoseWeigelin, BettinaEich, Christinade Vries, I. JolandaFigdor, Carl G.ImagingNanocarriersBiocompatible And Biodegradable PolymersNanomaterialsContrast AgentsFluorescenceMagnetic Resonance ImagingDendritic Cellshttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Dendritic cells (DCs) are the most potent antigen-presenting cells (APCs), involved in the induction of immunity and currently exploited for antitumor immunotherapies. An optimized noninvasive imaging modality capable of determining and quantifying DC-targeted nanoparticle (NP) trajectories could provide valuable information regarding therapeutic vaccine outcome. Here, targeted poly(d,l-lactide-co-glycolide) nanoparticles (PLGA NPs) recognizing DC receptors were equipped with superparamagnetic iron oxide particles (SPIO) or gold nanoparticles with fluorescently labeled antigen. The fluorescent label allowed for rapid analysis and quantification of DC-specific uptake of targeted PLGA NPs in comparison to uptake by other cells. Transmission electron microscopy (TEM) showed that a fraction of the encapsulated antigen reached the lysosomal compartment of DCs, where SPIO and gold were already partially released. However, part of the PLGA NPs localized within the cytoplasm, as confirmed by confocal microscopy. DCs targeted with NPs carrying SPIO or fluorescent antigen were detected within lymph nodes as early as 1 h after injection by magnetic resonance imaging (MRI). Despite the fact that targeting did not markedly affect PLGA NP biodistribution on organism and tissue level, it increased delivery of NPs to DCs residing in peripheral lymph nodes and resulted in enhanced T cell proliferation. In conclusion, two imaging agents within a single carrier allows tracking of targeted PLGA NPs at the subcellular, cellular, and organismal levels, thereby facilitating the rational design of in vivo targeted vaccination strategies.Fil: Cruz, Luis J.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países BajosFil: Tacken, Paul J.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países BajosFil: Zeelenberg, Ingrid S.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países BajosFil: Srinivas, Mangala. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países BajosFil: Bonetto, Fernando Jose. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Física del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); ArgentinaFil: Weigelin, Bettina. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Cell Biology; Países BajosFil: Eich, Christina. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países BajosFil: de Vries, I. Jolanda. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países BajosFil: Figdor, Carl G.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países BajosAmerican Chemical Society2014-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4433Cruz, Luis J.; Tacken, Paul J.; Zeelenberg, Ingrid S.; Srinivas, Mangala; Bonetto, Fernando Jose; et al.; Tracking Targeted Bimodal Nanovaccines: Immune Responses and Routing in Cells, Tissue, and Whole Organism; American Chemical Society; Molecular Pharmaceutics; 11; 12; 10-2014; 4299–43131543-8384enginfo:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/mp400717rinfo:eu-repo/semantics/altIdentifier/doi/DOI:10.1021/mp400717rinfo:eu-repo/semantics/altIdentifier/issn/1543-8384info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:22Zoai:ri.conicet.gov.ar:11336/4433instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:23.143CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Tracking Targeted Bimodal Nanovaccines: Immune Responses and Routing in Cells, Tissue, and Whole Organism |
title |
Tracking Targeted Bimodal Nanovaccines: Immune Responses and Routing in Cells, Tissue, and Whole Organism |
spellingShingle |
Tracking Targeted Bimodal Nanovaccines: Immune Responses and Routing in Cells, Tissue, and Whole Organism Cruz, Luis J. Imaging Nanocarriers Biocompatible And Biodegradable Polymers Nanomaterials Contrast Agents Fluorescence Magnetic Resonance Imaging Dendritic Cells |
title_short |
Tracking Targeted Bimodal Nanovaccines: Immune Responses and Routing in Cells, Tissue, and Whole Organism |
title_full |
Tracking Targeted Bimodal Nanovaccines: Immune Responses and Routing in Cells, Tissue, and Whole Organism |
title_fullStr |
Tracking Targeted Bimodal Nanovaccines: Immune Responses and Routing in Cells, Tissue, and Whole Organism |
title_full_unstemmed |
Tracking Targeted Bimodal Nanovaccines: Immune Responses and Routing in Cells, Tissue, and Whole Organism |
title_sort |
Tracking Targeted Bimodal Nanovaccines: Immune Responses and Routing in Cells, Tissue, and Whole Organism |
dc.creator.none.fl_str_mv |
Cruz, Luis J. Tacken, Paul J. Zeelenberg, Ingrid S. Srinivas, Mangala Bonetto, Fernando Jose Weigelin, Bettina Eich, Christina de Vries, I. Jolanda Figdor, Carl G. |
author |
Cruz, Luis J. |
author_facet |
Cruz, Luis J. Tacken, Paul J. Zeelenberg, Ingrid S. Srinivas, Mangala Bonetto, Fernando Jose Weigelin, Bettina Eich, Christina de Vries, I. Jolanda Figdor, Carl G. |
author_role |
author |
author2 |
Tacken, Paul J. Zeelenberg, Ingrid S. Srinivas, Mangala Bonetto, Fernando Jose Weigelin, Bettina Eich, Christina de Vries, I. Jolanda Figdor, Carl G. |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
Imaging Nanocarriers Biocompatible And Biodegradable Polymers Nanomaterials Contrast Agents Fluorescence Magnetic Resonance Imaging Dendritic Cells |
topic |
Imaging Nanocarriers Biocompatible And Biodegradable Polymers Nanomaterials Contrast Agents Fluorescence Magnetic Resonance Imaging Dendritic Cells |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.4 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Dendritic cells (DCs) are the most potent antigen-presenting cells (APCs), involved in the induction of immunity and currently exploited for antitumor immunotherapies. An optimized noninvasive imaging modality capable of determining and quantifying DC-targeted nanoparticle (NP) trajectories could provide valuable information regarding therapeutic vaccine outcome. Here, targeted poly(d,l-lactide-co-glycolide) nanoparticles (PLGA NPs) recognizing DC receptors were equipped with superparamagnetic iron oxide particles (SPIO) or gold nanoparticles with fluorescently labeled antigen. The fluorescent label allowed for rapid analysis and quantification of DC-specific uptake of targeted PLGA NPs in comparison to uptake by other cells. Transmission electron microscopy (TEM) showed that a fraction of the encapsulated antigen reached the lysosomal compartment of DCs, where SPIO and gold were already partially released. However, part of the PLGA NPs localized within the cytoplasm, as confirmed by confocal microscopy. DCs targeted with NPs carrying SPIO or fluorescent antigen were detected within lymph nodes as early as 1 h after injection by magnetic resonance imaging (MRI). Despite the fact that targeting did not markedly affect PLGA NP biodistribution on organism and tissue level, it increased delivery of NPs to DCs residing in peripheral lymph nodes and resulted in enhanced T cell proliferation. In conclusion, two imaging agents within a single carrier allows tracking of targeted PLGA NPs at the subcellular, cellular, and organismal levels, thereby facilitating the rational design of in vivo targeted vaccination strategies. Fil: Cruz, Luis J.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos Fil: Tacken, Paul J.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos Fil: Zeelenberg, Ingrid S.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos Fil: Srinivas, Mangala. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos Fil: Bonetto, Fernando Jose. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Física del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina Fil: Weigelin, Bettina. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Cell Biology; Países Bajos Fil: Eich, Christina. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos Fil: de Vries, I. Jolanda. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos Fil: Figdor, Carl G.. Radboud University Medical Center. Radboud Institute for Molecular Life Sciences. Department of Tumor Immunology; Países Bajos |
description |
Dendritic cells (DCs) are the most potent antigen-presenting cells (APCs), involved in the induction of immunity and currently exploited for antitumor immunotherapies. An optimized noninvasive imaging modality capable of determining and quantifying DC-targeted nanoparticle (NP) trajectories could provide valuable information regarding therapeutic vaccine outcome. Here, targeted poly(d,l-lactide-co-glycolide) nanoparticles (PLGA NPs) recognizing DC receptors were equipped with superparamagnetic iron oxide particles (SPIO) or gold nanoparticles with fluorescently labeled antigen. The fluorescent label allowed for rapid analysis and quantification of DC-specific uptake of targeted PLGA NPs in comparison to uptake by other cells. Transmission electron microscopy (TEM) showed that a fraction of the encapsulated antigen reached the lysosomal compartment of DCs, where SPIO and gold were already partially released. However, part of the PLGA NPs localized within the cytoplasm, as confirmed by confocal microscopy. DCs targeted with NPs carrying SPIO or fluorescent antigen were detected within lymph nodes as early as 1 h after injection by magnetic resonance imaging (MRI). Despite the fact that targeting did not markedly affect PLGA NP biodistribution on organism and tissue level, it increased delivery of NPs to DCs residing in peripheral lymph nodes and resulted in enhanced T cell proliferation. In conclusion, two imaging agents within a single carrier allows tracking of targeted PLGA NPs at the subcellular, cellular, and organismal levels, thereby facilitating the rational design of in vivo targeted vaccination strategies. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/4433 Cruz, Luis J.; Tacken, Paul J.; Zeelenberg, Ingrid S.; Srinivas, Mangala; Bonetto, Fernando Jose; et al.; Tracking Targeted Bimodal Nanovaccines: Immune Responses and Routing in Cells, Tissue, and Whole Organism; American Chemical Society; Molecular Pharmaceutics; 11; 12; 10-2014; 4299–4313 1543-8384 |
url |
http://hdl.handle.net/11336/4433 |
identifier_str_mv |
Cruz, Luis J.; Tacken, Paul J.; Zeelenberg, Ingrid S.; Srinivas, Mangala; Bonetto, Fernando Jose; et al.; Tracking Targeted Bimodal Nanovaccines: Immune Responses and Routing in Cells, Tissue, and Whole Organism; American Chemical Society; Molecular Pharmaceutics; 11; 12; 10-2014; 4299–4313 1543-8384 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/mp400717r info:eu-repo/semantics/altIdentifier/doi/DOI:10.1021/mp400717r info:eu-repo/semantics/altIdentifier/issn/1543-8384 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
American Chemical Society |
publisher.none.fl_str_mv |
American Chemical Society |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.13397 |