Multimodal imaging of nanovaccine carriers targeted to human dendritic cells

Autores
Cruz, Luis; Tacken, Paul; Bonetto, Fernando Jose; Buschow, Sonja; Croes, Huib; Wijers, Miestke; de Vries, Jolanda; Figdor, Carl
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Dendritic cells (DCs) are key players in the initiation of adaptive immune responses and are currently exploited in immunotherapy against cancer and infectious diseases. The targeted delivery of nanovaccine particles (NPs) to DCs in vivo is a promising strategy to enhance immune
responses. Here, targeted nanovaccine carriers were generated that allow multimodal imaging of nanocarrierDC interactions from the subcellular to the organism level. These carriers were
made of biodegradable poly(D,L-lactide-co-glycolide) harboring superparamagnetic iron oxide particles (SPIO) and fluorescently labeled antigen in a single particle. Targeted delivery was
facilitated by coating the NPs with antibodies recognizing the DC-specific receptor DC-SIGN. The fluorescent label allowed for rapid analysis and quantification of specific versus nonspecific uptake of targeted NPs by DCs compared to other blood cells. In addition, it showed that part of the encapsulated antigen reached the lysosomal compartment of DCs within 24 h. Moreover, the
presence of fluorescent label did not prevent the antigen from being presented to antigen-specific T cells. The incorporated SPIO was applied to track the NPs at subcellular cell organel level using transmission electron microscopy (TEM). NPs were found within endolysosomal compartments, where part of the SPIO was already released within 24 h. Furthermore, part of the NPs seemed to
localize within the cytoplasm. Ex vivo loading of DCs with NPs resulted in efficient labeling and  detection by MRI and did not abolish cell migration within collagen scaffolds. In conclusion,  incorporation of two imaging agents within a single carrier allows tracking of targeted nanovaccines on a subcellular, cellular and possibly organism level, thereby facilitating rational design of in vivo targeted vaccination strategies.
Fil: Cruz, Luis. University Medical Centre; Países Bajos
Fil: Tacken, Paul. University Medical Centre; Países Bajos
Fil: Bonetto, Fernando Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina
Fil: Buschow, Sonja. University Medical Centre; Países Bajos
Fil: Croes, Huib. Radboud University Nijmegen Medical Centre; Países Bajos
Fil: Wijers, Miestke. Radboud University Nijmegen Medical Centre; Países Bajos
Fil: de Vries, Jolanda. University Medical Centre; Países Bajos
Fil: Figdor, Carl. University Medical Centre; Países Bajos
Materia
Imaging
Nanocarriers
Biocompatible Materials
Contrast Agents
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13548
Acceder
id CONICETDig_23bb21373d069086f9b764a329154163
oai_identifier_str oai:ri.conicet.gov.ar:11336/13548
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Multimodal imaging of nanovaccine carriers targeted to human dendritic cellsCruz, LuisTacken, PaulBonetto, Fernando JoseBuschow, SonjaCroes, HuibWijers, Miestkede Vries, JolandaFigdor, CarlImagingNanocarriersBiocompatible MaterialsContrast Agentshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Dendritic cells (DCs) are key players in the initiation of adaptive immune responses and are currently exploited in immunotherapy against cancer and infectious diseases. The targeted delivery of nanovaccine particles (NPs) to DCs in vivo is a promising strategy to enhance immune<br />responses. Here, targeted nanovaccine carriers were generated that allow multimodal imaging of nanocarrierDC interactions from the subcellular to the organism level. These carriers were<br />made of biodegradable poly(D,L-lactide-co-glycolide) harboring superparamagnetic iron oxide particles (SPIO) and fluorescently labeled antigen in a single particle. Targeted delivery was<br />facilitated by coating the NPs with antibodies recognizing the DC-specific receptor DC-SIGN. The fluorescent label allowed for rapid analysis and quantification of specific versus nonspecific uptake of targeted NPs by DCs compared to other blood cells. In addition, it showed that part of the encapsulated antigen reached the lysosomal compartment of DCs within 24 h. Moreover, the<br />presence of fluorescent label did not prevent the antigen from being presented to antigen-specific T cells. The incorporated SPIO was applied to track the NPs at subcellular cell organel level using transmission electron microscopy (TEM). NPs were found within endolysosomal compartments, where part of the SPIO was already released within 24 h. Furthermore, part of the NPs seemed to<br />localize within the cytoplasm. Ex vivo loading of DCs with NPs resulted in efficient labeling and  detection by MRI and did not abolish cell migration within collagen scaffolds. In conclusion,  incorporation of two imaging agents within a single carrier allows tracking of targeted nanovaccines on a subcellular, cellular and possibly organism level, thereby facilitating rational design of in vivo targeted vaccination strategies.Fil: Cruz, Luis. University Medical Centre; Países BajosFil: Tacken, Paul. University Medical Centre; Países BajosFil: Bonetto, Fernando Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); ArgentinaFil: Buschow, Sonja. University Medical Centre; Países BajosFil: Croes, Huib. Radboud University Nijmegen Medical Centre; Países BajosFil: Wijers, Miestke. Radboud University Nijmegen Medical Centre; Países BajosFil: de Vries, Jolanda. University Medical Centre; Países BajosFil: Figdor, Carl. University Medical Centre; Países BajosAmerican Chemical Society2011-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13548Cruz, Luis; Tacken, Paul; Bonetto, Fernando Jose; Buschow, Sonja; Croes, Huib; et al.; Multimodal imaging of nanovaccine carriers targeted to human dendritic cells; American Chemical Society; Molecular Pharmaceutics; 8; 3-2011; 520-5311543-8384enginfo:eu-repo/semantics/altIdentifier/doi/dx.doi.org/10.1021/mp100356kinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:58:17Zoai:ri.conicet.gov.ar:11336/13548instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:58:18.159CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Multimodal imaging of nanovaccine carriers targeted to human dendritic cells
title Multimodal imaging of nanovaccine carriers targeted to human dendritic cells
spellingShingle Multimodal imaging of nanovaccine carriers targeted to human dendritic cells
Cruz, Luis
Imaging
Nanocarriers
Biocompatible Materials
Contrast Agents
title_short Multimodal imaging of nanovaccine carriers targeted to human dendritic cells
title_full Multimodal imaging of nanovaccine carriers targeted to human dendritic cells
title_fullStr Multimodal imaging of nanovaccine carriers targeted to human dendritic cells
title_full_unstemmed Multimodal imaging of nanovaccine carriers targeted to human dendritic cells
title_sort Multimodal imaging of nanovaccine carriers targeted to human dendritic cells
dc.creator.none.fl_str_mv Cruz, Luis
Tacken, Paul
Bonetto, Fernando Jose
Buschow, Sonja
Croes, Huib
Wijers, Miestke
de Vries, Jolanda
Figdor, Carl
author Cruz, Luis
author_facet Cruz, Luis
Tacken, Paul
Bonetto, Fernando Jose
Buschow, Sonja
Croes, Huib
Wijers, Miestke
de Vries, Jolanda
Figdor, Carl
author_role author
author2 Tacken, Paul
Bonetto, Fernando Jose
Buschow, Sonja
Croes, Huib
Wijers, Miestke
de Vries, Jolanda
Figdor, Carl
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Imaging
Nanocarriers
Biocompatible Materials
Contrast Agents
topic Imaging
Nanocarriers
Biocompatible Materials
Contrast Agents
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Dendritic cells (DCs) are key players in the initiation of adaptive immune responses and are currently exploited in immunotherapy against cancer and infectious diseases. The targeted delivery of nanovaccine particles (NPs) to DCs in vivo is a promising strategy to enhance immune<br />responses. Here, targeted nanovaccine carriers were generated that allow multimodal imaging of nanocarrierDC interactions from the subcellular to the organism level. These carriers were<br />made of biodegradable poly(D,L-lactide-co-glycolide) harboring superparamagnetic iron oxide particles (SPIO) and fluorescently labeled antigen in a single particle. Targeted delivery was<br />facilitated by coating the NPs with antibodies recognizing the DC-specific receptor DC-SIGN. The fluorescent label allowed for rapid analysis and quantification of specific versus nonspecific uptake of targeted NPs by DCs compared to other blood cells. In addition, it showed that part of the encapsulated antigen reached the lysosomal compartment of DCs within 24 h. Moreover, the<br />presence of fluorescent label did not prevent the antigen from being presented to antigen-specific T cells. The incorporated SPIO was applied to track the NPs at subcellular cell organel level using transmission electron microscopy (TEM). NPs were found within endolysosomal compartments, where part of the SPIO was already released within 24 h. Furthermore, part of the NPs seemed to<br />localize within the cytoplasm. Ex vivo loading of DCs with NPs resulted in efficient labeling and  detection by MRI and did not abolish cell migration within collagen scaffolds. In conclusion,  incorporation of two imaging agents within a single carrier allows tracking of targeted nanovaccines on a subcellular, cellular and possibly organism level, thereby facilitating rational design of in vivo targeted vaccination strategies.
Fil: Cruz, Luis. University Medical Centre; Países Bajos
Fil: Tacken, Paul. University Medical Centre; Países Bajos
Fil: Bonetto, Fernando Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina
Fil: Buschow, Sonja. University Medical Centre; Países Bajos
Fil: Croes, Huib. Radboud University Nijmegen Medical Centre; Países Bajos
Fil: Wijers, Miestke. Radboud University Nijmegen Medical Centre; Países Bajos
Fil: de Vries, Jolanda. University Medical Centre; Países Bajos
Fil: Figdor, Carl. University Medical Centre; Países Bajos
description Dendritic cells (DCs) are key players in the initiation of adaptive immune responses and are currently exploited in immunotherapy against cancer and infectious diseases. The targeted delivery of nanovaccine particles (NPs) to DCs in vivo is a promising strategy to enhance immune<br />responses. Here, targeted nanovaccine carriers were generated that allow multimodal imaging of nanocarrierDC interactions from the subcellular to the organism level. These carriers were<br />made of biodegradable poly(D,L-lactide-co-glycolide) harboring superparamagnetic iron oxide particles (SPIO) and fluorescently labeled antigen in a single particle. Targeted delivery was<br />facilitated by coating the NPs with antibodies recognizing the DC-specific receptor DC-SIGN. The fluorescent label allowed for rapid analysis and quantification of specific versus nonspecific uptake of targeted NPs by DCs compared to other blood cells. In addition, it showed that part of the encapsulated antigen reached the lysosomal compartment of DCs within 24 h. Moreover, the<br />presence of fluorescent label did not prevent the antigen from being presented to antigen-specific T cells. The incorporated SPIO was applied to track the NPs at subcellular cell organel level using transmission electron microscopy (TEM). NPs were found within endolysosomal compartments, where part of the SPIO was already released within 24 h. Furthermore, part of the NPs seemed to<br />localize within the cytoplasm. Ex vivo loading of DCs with NPs resulted in efficient labeling and  detection by MRI and did not abolish cell migration within collagen scaffolds. In conclusion,  incorporation of two imaging agents within a single carrier allows tracking of targeted nanovaccines on a subcellular, cellular and possibly organism level, thereby facilitating rational design of in vivo targeted vaccination strategies.
publishDate 2011
dc.date.none.fl_str_mv 2011-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13548
Cruz, Luis; Tacken, Paul; Bonetto, Fernando Jose; Buschow, Sonja; Croes, Huib; et al.; Multimodal imaging of nanovaccine carriers targeted to human dendritic cells; American Chemical Society; Molecular Pharmaceutics; 8; 3-2011; 520-531
1543-8384
url http://hdl.handle.net/11336/13548
identifier_str_mv Cruz, Luis; Tacken, Paul; Bonetto, Fernando Jose; Buschow, Sonja; Croes, Huib; et al.; Multimodal imaging of nanovaccine carriers targeted to human dendritic cells; American Chemical Society; Molecular Pharmaceutics; 8; 3-2011; 520-531
1543-8384
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/dx.doi.org/10.1021/mp100356k
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083123101564928
score 13.22299

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