Postembryonic development and aging of the appendicular skeleton in Ambystoma mexicanum

Autores
Riquelme Guzmán, Camilo; Schuez, Maritta; Böhm, Alexander; Knapp, Dunja; Edwards Jorquera, Sandra; Ceccarelli, Alberto Sebastián; Chara, Osvaldo; Rauner, Martina; Sandoval Guzmán, Tatiana
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: The axolotl is a key model to study appendicular regeneration. The limb complexity resembles that of humans in structure and tissue components; however, axolotl limbs develop postembryonically. In this work, we evaluated the postembryonic development of the appendicular skeleton and its changes with aging. Results: The juvenile limb skeleton is formed mostly by Sox9/Col1a2 cartilage cells. Ossification of the appendicular skeleton starts when animals reach a length of 10 cm, and cartilage cells are replaced by a primary ossification center, consisting of cortical bone and an adipocyte-filled marrow cavity. Vascularization is associated with the ossification center and the marrow cavity formation. We identified the contribution of Col1a2-descendants to bone and adipocytes. Moreover, ossification progresses with age toward the epiphyses of long bones. Axolotls are neotenic salamanders, and still ossification remains responsive to l-thyroxine, increasing the rate of bone formation. Conclusions: In axolotls, bone maturation is a continuous process that extends throughout their life. Ossification of the appendicular bones is slow and continues until the complete element is ossified. The cellular components of the appendicular skeleton change accordingly during ossification, creating a heterogenous landscape in each element. The continuous maturation of the bone is accompanied by a continuous body growth.
Fil: Riquelme Guzmán, Camilo. Technische Universität Dresden; Alemania
Fil: Schuez, Maritta. Technische Universität Dresden; Alemania
Fil: Böhm, Alexander. Technische Universität Dresden; Alemania
Fil: Knapp, Dunja. Technische Universität Dresden; Alemania
Fil: Edwards Jorquera, Sandra. Technische Universität Dresden; Alemania
Fil: Ceccarelli, Alberto Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; Argentina
Fil: Chara, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Argentina de la Empresa; Argentina. Technische Universität Dresden; Alemania
Fil: Rauner, Martina. Universitätsklinikum Carl Gustav Carus; Alemania
Fil: Sandoval Guzmán, Tatiana. Universitätsklinikum Carl Gustav Carus; Alemania. Technische Universität Dresden; Alemania
Materia
AGING
AXOLOTL
CHONDROCYTES
OSSIFICATION
OSTEOBLASTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/212669
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/212669
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Postembryonic development and aging of the appendicular skeleton in Ambystoma mexicanumRiquelme Guzmán, CamiloSchuez, MarittaBöhm, AlexanderKnapp, DunjaEdwards Jorquera, SandraCeccarelli, Alberto SebastiánChara, OsvaldoRauner, MartinaSandoval Guzmán, TatianaAGINGAXOLOTLCHONDROCYTESOSSIFICATIONOSTEOBLASTShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: The axolotl is a key model to study appendicular regeneration. The limb complexity resembles that of humans in structure and tissue components; however, axolotl limbs develop postembryonically. In this work, we evaluated the postembryonic development of the appendicular skeleton and its changes with aging. Results: The juvenile limb skeleton is formed mostly by Sox9/Col1a2 cartilage cells. Ossification of the appendicular skeleton starts when animals reach a length of 10 cm, and cartilage cells are replaced by a primary ossification center, consisting of cortical bone and an adipocyte-filled marrow cavity. Vascularization is associated with the ossification center and the marrow cavity formation. We identified the contribution of Col1a2-descendants to bone and adipocytes. Moreover, ossification progresses with age toward the epiphyses of long bones. Axolotls are neotenic salamanders, and still ossification remains responsive to l-thyroxine, increasing the rate of bone formation. Conclusions: In axolotls, bone maturation is a continuous process that extends throughout their life. Ossification of the appendicular bones is slow and continues until the complete element is ossified. The cellular components of the appendicular skeleton change accordingly during ossification, creating a heterogenous landscape in each element. The continuous maturation of the bone is accompanied by a continuous body growth.Fil: Riquelme Guzmán, Camilo. Technische Universität Dresden; AlemaniaFil: Schuez, Maritta. Technische Universität Dresden; AlemaniaFil: Böhm, Alexander. Technische Universität Dresden; AlemaniaFil: Knapp, Dunja. Technische Universität Dresden; AlemaniaFil: Edwards Jorquera, Sandra. Technische Universität Dresden; AlemaniaFil: Ceccarelli, Alberto Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; ArgentinaFil: Chara, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Argentina de la Empresa; Argentina. Technische Universität Dresden; AlemaniaFil: Rauner, Martina. Universitätsklinikum Carl Gustav Carus; AlemaniaFil: Sandoval Guzmán, Tatiana. Universitätsklinikum Carl Gustav Carus; Alemania. Technische Universität Dresden; AlemaniaWiley-liss, div John Wiley & Sons Inc.2022-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/212669Riquelme Guzmán, Camilo; Schuez, Maritta; Böhm, Alexander; Knapp, Dunja; Edwards Jorquera, Sandra; et al.; Postembryonic development and aging of the appendicular skeleton in Ambystoma mexicanum; Wiley-liss, div John Wiley & Sons Inc.; Developmental Dynamics; 251; 6; 6-2022; 1015-10341058-8388CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/dvdy.407info:eu-repo/semantics/altIdentifier/url/https://anatomypubs.onlinelibrary.wiley.com/doi/10.1002/dvdy.407info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:08:45Zoai:ri.conicet.gov.ar:11336/212669instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:08:45.75CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Postembryonic development and aging of the appendicular skeleton in Ambystoma mexicanum
title Postembryonic development and aging of the appendicular skeleton in Ambystoma mexicanum
spellingShingle Postembryonic development and aging of the appendicular skeleton in Ambystoma mexicanum
Riquelme Guzmán, Camilo
AGING
AXOLOTL
CHONDROCYTES
OSSIFICATION
OSTEOBLASTS
title_short Postembryonic development and aging of the appendicular skeleton in Ambystoma mexicanum
title_full Postembryonic development and aging of the appendicular skeleton in Ambystoma mexicanum
title_fullStr Postembryonic development and aging of the appendicular skeleton in Ambystoma mexicanum
title_full_unstemmed Postembryonic development and aging of the appendicular skeleton in Ambystoma mexicanum
title_sort Postembryonic development and aging of the appendicular skeleton in Ambystoma mexicanum
dc.creator.none.fl_str_mv Riquelme Guzmán, Camilo
Schuez, Maritta
Böhm, Alexander
Knapp, Dunja
Edwards Jorquera, Sandra
Ceccarelli, Alberto Sebastián
Chara, Osvaldo
Rauner, Martina
Sandoval Guzmán, Tatiana
author Riquelme Guzmán, Camilo
author_facet Riquelme Guzmán, Camilo
Schuez, Maritta
Böhm, Alexander
Knapp, Dunja
Edwards Jorquera, Sandra
Ceccarelli, Alberto Sebastián
Chara, Osvaldo
Rauner, Martina
Sandoval Guzmán, Tatiana
author_role author
author2 Schuez, Maritta
Böhm, Alexander
Knapp, Dunja
Edwards Jorquera, Sandra
Ceccarelli, Alberto Sebastián
Chara, Osvaldo
Rauner, Martina
Sandoval Guzmán, Tatiana
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AGING
AXOLOTL
CHONDROCYTES
OSSIFICATION
OSTEOBLASTS
topic AGING
AXOLOTL
CHONDROCYTES
OSSIFICATION
OSTEOBLASTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: The axolotl is a key model to study appendicular regeneration. The limb complexity resembles that of humans in structure and tissue components; however, axolotl limbs develop postembryonically. In this work, we evaluated the postembryonic development of the appendicular skeleton and its changes with aging. Results: The juvenile limb skeleton is formed mostly by Sox9/Col1a2 cartilage cells. Ossification of the appendicular skeleton starts when animals reach a length of 10 cm, and cartilage cells are replaced by a primary ossification center, consisting of cortical bone and an adipocyte-filled marrow cavity. Vascularization is associated with the ossification center and the marrow cavity formation. We identified the contribution of Col1a2-descendants to bone and adipocytes. Moreover, ossification progresses with age toward the epiphyses of long bones. Axolotls are neotenic salamanders, and still ossification remains responsive to l-thyroxine, increasing the rate of bone formation. Conclusions: In axolotls, bone maturation is a continuous process that extends throughout their life. Ossification of the appendicular bones is slow and continues until the complete element is ossified. The cellular components of the appendicular skeleton change accordingly during ossification, creating a heterogenous landscape in each element. The continuous maturation of the bone is accompanied by a continuous body growth.
Fil: Riquelme Guzmán, Camilo. Technische Universität Dresden; Alemania
Fil: Schuez, Maritta. Technische Universität Dresden; Alemania
Fil: Böhm, Alexander. Technische Universität Dresden; Alemania
Fil: Knapp, Dunja. Technische Universität Dresden; Alemania
Fil: Edwards Jorquera, Sandra. Technische Universität Dresden; Alemania
Fil: Ceccarelli, Alberto Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; Argentina
Fil: Chara, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Argentina de la Empresa; Argentina. Technische Universität Dresden; Alemania
Fil: Rauner, Martina. Universitätsklinikum Carl Gustav Carus; Alemania
Fil: Sandoval Guzmán, Tatiana. Universitätsklinikum Carl Gustav Carus; Alemania. Technische Universität Dresden; Alemania
description Background: The axolotl is a key model to study appendicular regeneration. The limb complexity resembles that of humans in structure and tissue components; however, axolotl limbs develop postembryonically. In this work, we evaluated the postembryonic development of the appendicular skeleton and its changes with aging. Results: The juvenile limb skeleton is formed mostly by Sox9/Col1a2 cartilage cells. Ossification of the appendicular skeleton starts when animals reach a length of 10 cm, and cartilage cells are replaced by a primary ossification center, consisting of cortical bone and an adipocyte-filled marrow cavity. Vascularization is associated with the ossification center and the marrow cavity formation. We identified the contribution of Col1a2-descendants to bone and adipocytes. Moreover, ossification progresses with age toward the epiphyses of long bones. Axolotls are neotenic salamanders, and still ossification remains responsive to l-thyroxine, increasing the rate of bone formation. Conclusions: In axolotls, bone maturation is a continuous process that extends throughout their life. Ossification of the appendicular bones is slow and continues until the complete element is ossified. The cellular components of the appendicular skeleton change accordingly during ossification, creating a heterogenous landscape in each element. The continuous maturation of the bone is accompanied by a continuous body growth.
publishDate 2022
dc.date.none.fl_str_mv 2022-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/212669
Riquelme Guzmán, Camilo; Schuez, Maritta; Böhm, Alexander; Knapp, Dunja; Edwards Jorquera, Sandra; et al.; Postembryonic development and aging of the appendicular skeleton in Ambystoma mexicanum; Wiley-liss, div John Wiley & Sons Inc.; Developmental Dynamics; 251; 6; 6-2022; 1015-1034
1058-8388
CONICET Digital
CONICET
url http://hdl.handle.net/11336/212669
identifier_str_mv Riquelme Guzmán, Camilo; Schuez, Maritta; Böhm, Alexander; Knapp, Dunja; Edwards Jorquera, Sandra; et al.; Postembryonic development and aging of the appendicular skeleton in Ambystoma mexicanum; Wiley-liss, div John Wiley & Sons Inc.; Developmental Dynamics; 251; 6; 6-2022; 1015-1034
1058-8388
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/dvdy.407
info:eu-repo/semantics/altIdentifier/url/https://anatomypubs.onlinelibrary.wiley.com/doi/10.1002/dvdy.407
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, div John Wiley & Sons Inc.
publisher.none.fl_str_mv Wiley-liss, div John Wiley & Sons Inc.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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