HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: Synthesis and biological bharacterization
- Autores
- Torres, María Luz; Oberti, Tamara Gisela; Fernández, Juan Manuel
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Cartilage tissue engineering (CTE) has the general objective of restoring and improving damaged cartilage. A very interesting strategy of CTE is to combine different polymers to obtain a viscoelastic material. In the present study we have evaluated the applicability of Poly(2-hydroxyethyl methacrylate) networks semi-interpenetrated with sodium alginate for CTE. Alginate-containing hydrogels show an increase in scaffold porosity and swelling capacity, when compared with nonporous poly(2-hydroxyethyl methacrylate) scaffolds. Primary chondrocytes from young rats were cultured on the hydrogels, and an increase in chondrocyte proliferation and chondrocytic markers was observed in alginate-containing hydrogels. Chondrocytic phenotype was preserved on hydrogels containing the lowest amount of crosslinker and initiator (SEMI 3 and SEMI 4). In addition, Nitric oxide production by RAW264.7 macrophages grown on hydrogels was tested and none of the hydrogels showed high levels of this inflammatory marker after 2 days. These results indicate that our alginate-containing hydrogels could be useful for CTE.
Fil: Torres, María Luz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina
Fil: Oberti, Tamara Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Fernández, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina - Materia
-
SEMI-INTERPENETRATING POLYMER NETWORK
CHONDROCYTE
CHONDROCOMPATIBILITY
SWELLING
CONTACT ANGLE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/139939
Ver los metadatos del registro completo
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CONICET Digital (CONICET) |
spelling |
HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: Synthesis and biological bharacterizationTorres, María LuzOberti, Tamara GiselaFernández, Juan ManuelSEMI-INTERPENETRATING POLYMER NETWORKCHONDROCYTECHONDROCOMPATIBILITYSWELLINGCONTACT ANGLEhttps://purl.org/becyt/ford/2.6https://purl.org/becyt/ford/2Cartilage tissue engineering (CTE) has the general objective of restoring and improving damaged cartilage. A very interesting strategy of CTE is to combine different polymers to obtain a viscoelastic material. In the present study we have evaluated the applicability of Poly(2-hydroxyethyl methacrylate) networks semi-interpenetrated with sodium alginate for CTE. Alginate-containing hydrogels show an increase in scaffold porosity and swelling capacity, when compared with nonporous poly(2-hydroxyethyl methacrylate) scaffolds. Primary chondrocytes from young rats were cultured on the hydrogels, and an increase in chondrocyte proliferation and chondrocytic markers was observed in alginate-containing hydrogels. Chondrocytic phenotype was preserved on hydrogels containing the lowest amount of crosslinker and initiator (SEMI 3 and SEMI 4). In addition, Nitric oxide production by RAW264.7 macrophages grown on hydrogels was tested and none of the hydrogels showed high levels of this inflammatory marker after 2 days. These results indicate that our alginate-containing hydrogels could be useful for CTE.Fil: Torres, María Luz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; ArgentinaFil: Oberti, Tamara Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Fernández, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; ArgentinaTaylor & Francis2020-11-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/139939Torres, María Luz; Oberti, Tamara Gisela; Fernández, Juan Manuel; HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: Synthesis and biological bharacterization; Taylor & Francis; Journal of Biomaterials Science - Polymer Edition; 32; 4; 12-11-2020; 504-5230920-5063CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/09205063.2020.1849920info:eu-repo/semantics/altIdentifier/doi/10.1080/09205063.2020.1849920info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:42:51Zoai:ri.conicet.gov.ar:11336/139939instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:42:51.769CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: Synthesis and biological bharacterization |
title |
HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: Synthesis and biological bharacterization |
spellingShingle |
HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: Synthesis and biological bharacterization Torres, María Luz SEMI-INTERPENETRATING POLYMER NETWORK CHONDROCYTE CHONDROCOMPATIBILITY SWELLING CONTACT ANGLE |
title_short |
HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: Synthesis and biological bharacterization |
title_full |
HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: Synthesis and biological bharacterization |
title_fullStr |
HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: Synthesis and biological bharacterization |
title_full_unstemmed |
HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: Synthesis and biological bharacterization |
title_sort |
HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: Synthesis and biological bharacterization |
dc.creator.none.fl_str_mv |
Torres, María Luz Oberti, Tamara Gisela Fernández, Juan Manuel |
author |
Torres, María Luz |
author_facet |
Torres, María Luz Oberti, Tamara Gisela Fernández, Juan Manuel |
author_role |
author |
author2 |
Oberti, Tamara Gisela Fernández, Juan Manuel |
author2_role |
author author |
dc.subject.none.fl_str_mv |
SEMI-INTERPENETRATING POLYMER NETWORK CHONDROCYTE CHONDROCOMPATIBILITY SWELLING CONTACT ANGLE |
topic |
SEMI-INTERPENETRATING POLYMER NETWORK CHONDROCYTE CHONDROCOMPATIBILITY SWELLING CONTACT ANGLE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.6 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Cartilage tissue engineering (CTE) has the general objective of restoring and improving damaged cartilage. A very interesting strategy of CTE is to combine different polymers to obtain a viscoelastic material. In the present study we have evaluated the applicability of Poly(2-hydroxyethyl methacrylate) networks semi-interpenetrated with sodium alginate for CTE. Alginate-containing hydrogels show an increase in scaffold porosity and swelling capacity, when compared with nonporous poly(2-hydroxyethyl methacrylate) scaffolds. Primary chondrocytes from young rats were cultured on the hydrogels, and an increase in chondrocyte proliferation and chondrocytic markers was observed in alginate-containing hydrogels. Chondrocytic phenotype was preserved on hydrogels containing the lowest amount of crosslinker and initiator (SEMI 3 and SEMI 4). In addition, Nitric oxide production by RAW264.7 macrophages grown on hydrogels was tested and none of the hydrogels showed high levels of this inflammatory marker after 2 days. These results indicate that our alginate-containing hydrogels could be useful for CTE. Fil: Torres, María Luz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina Fil: Oberti, Tamara Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina Fil: Fernández, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; Argentina |
description |
Cartilage tissue engineering (CTE) has the general objective of restoring and improving damaged cartilage. A very interesting strategy of CTE is to combine different polymers to obtain a viscoelastic material. In the present study we have evaluated the applicability of Poly(2-hydroxyethyl methacrylate) networks semi-interpenetrated with sodium alginate for CTE. Alginate-containing hydrogels show an increase in scaffold porosity and swelling capacity, when compared with nonporous poly(2-hydroxyethyl methacrylate) scaffolds. Primary chondrocytes from young rats were cultured on the hydrogels, and an increase in chondrocyte proliferation and chondrocytic markers was observed in alginate-containing hydrogels. Chondrocytic phenotype was preserved on hydrogels containing the lowest amount of crosslinker and initiator (SEMI 3 and SEMI 4). In addition, Nitric oxide production by RAW264.7 macrophages grown on hydrogels was tested and none of the hydrogels showed high levels of this inflammatory marker after 2 days. These results indicate that our alginate-containing hydrogels could be useful for CTE. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/139939 Torres, María Luz; Oberti, Tamara Gisela; Fernández, Juan Manuel; HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: Synthesis and biological bharacterization; Taylor & Francis; Journal of Biomaterials Science - Polymer Edition; 32; 4; 12-11-2020; 504-523 0920-5063 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/139939 |
identifier_str_mv |
Torres, María Luz; Oberti, Tamara Gisela; Fernández, Juan Manuel; HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: Synthesis and biological bharacterization; Taylor & Francis; Journal of Biomaterials Science - Polymer Edition; 32; 4; 12-11-2020; 504-523 0920-5063 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/09205063.2020.1849920 info:eu-repo/semantics/altIdentifier/doi/10.1080/09205063.2020.1849920 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Taylor & Francis |
publisher.none.fl_str_mv |
Taylor & Francis |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613349328814080 |
score |
13.070432 |