Bifidobacterium animalis subsp lactis HN019 presents antimicrobial potential against periodontopathogens and modulates the immunological response of oral mucosa in periodontitis pa...

Autores
Invernici, Marcos M.; Furlaneto, Flávia A. C.; Salvador, Sérgio L; Ouwehand, Arthur C.; Salminen, Seppo; Mantziari, Anastasia; Vinderola, Celso Gabriel; Ervolino, Edilson; Santana, Sandro Isaías; Silva, Pedro Henrique Felix; Messora, Michel R.
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective To evaluate the effects of Bifidobacterium animalis subsp. lactis HN019 (HN019) on clinical periodontal parameters (plaque accumulation and gingival bleeding), on immunocompetence of gingival tissues [expression of beta-defensin (BD)-3, toll-like receptor 4 (TLR4), cluster of differentiation(CD)-57 and CD-4], and on immunological properties of saliva (IgA levels) in non-surgical periodontal therapy in generalized chronic periodontitis (GCP) patients. Adhesion to buccal epithelial cells (BEC) and the antimicrobial properties of HN019 were also investigated. Materials and methods Thirty patients were recruited and monitored clinically at baseline (before scaling and root planing-SRP) and after 30 and 90 days. Patients were randomly assigned to Test (SRP +Probiotic, n = 15) or Control (SRP+Placebo, n = 15) group. Probiotic lozenges were used for 30 days. Gingival tissues and saliva were immunologically analyzed. The adhesion of HN019 with or without Porphyromonas gingivalis in BEC and its antimicrobial properties were investigated in in vitro assays. Data were statistically analyzed (p<0.05). Results Test group presented lower plaque index (30 days) and lower marginal gingival bleeding (90 days) when compared with Control group. Higher BD-3, TLR4 and CD-4 expressions were observed in gingival tissues in Test group than in Control group. HN019 reduced the adhesion of P. gingivalis to BEC and showed antimicrobial potential against periodontopathogens. Conclusion Immunological and antimicrobial properties of B. lactis HN019 make it a potential probiotic to be used in non-surgical periodontal therapy of patients with GCP.
Fil: Invernici, Marcos M.. Universidade de Sao Paulo; Brasil
Fil: Furlaneto, Flávia A. C.. Universidade de Sao Paulo; Brasil
Fil: Salvador, Sérgio L. Universidade de Sao Paulo; Brasil
Fil: Ouwehand, Arthur C.. Dupont, Nutrition and Health; Finlandia
Fil: Salminen, Seppo. University of Turku. Functional Foods Forum; Finlandia
Fil: Mantziari, Anastasia. University of Turku. Functional Foods Forum; Finlandia
Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Ervolino, Edilson. São Paulo State University. Division of Histology, Department of Basic Sciences, Dental School of Araçatuba; Brasil
Fil: Santana, Sandro Isaías. Universidade de Sao Paulo; Brasil
Fil: Silva, Pedro Henrique Felix. Universidade de Sao Paulo; Brasil
Fil: Messora, Michel R.. Universidade de Sao Paulo; Brasil
Materia
Bifidobacterium
Periodontal
Probiotics
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/117098
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/117098
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bifidobacterium animalis subsp lactis HN019 presents antimicrobial potential against periodontopathogens and modulates the immunological response of oral mucosa in periodontitis patientsInvernici, Marcos M.Furlaneto, Flávia A. C.Salvador, Sérgio LOuwehand, Arthur C.Salminen, SeppoMantziari, AnastasiaVinderola, Celso GabrielErvolino, EdilsonSantana, Sandro IsaíasSilva, Pedro Henrique FelixMessora, Michel R.BifidobacteriumPeriodontalProbioticshttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Objective To evaluate the effects of Bifidobacterium animalis subsp. lactis HN019 (HN019) on clinical periodontal parameters (plaque accumulation and gingival bleeding), on immunocompetence of gingival tissues [expression of beta-defensin (BD)-3, toll-like receptor 4 (TLR4), cluster of differentiation(CD)-57 and CD-4], and on immunological properties of saliva (IgA levels) in non-surgical periodontal therapy in generalized chronic periodontitis (GCP) patients. Adhesion to buccal epithelial cells (BEC) and the antimicrobial properties of HN019 were also investigated. Materials and methods Thirty patients were recruited and monitored clinically at baseline (before scaling and root planing-SRP) and after 30 and 90 days. Patients were randomly assigned to Test (SRP +Probiotic, n = 15) or Control (SRP+Placebo, n = 15) group. Probiotic lozenges were used for 30 days. Gingival tissues and saliva were immunologically analyzed. The adhesion of HN019 with or without Porphyromonas gingivalis in BEC and its antimicrobial properties were investigated in in vitro assays. Data were statistically analyzed (p<0.05). Results Test group presented lower plaque index (30 days) and lower marginal gingival bleeding (90 days) when compared with Control group. Higher BD-3, TLR4 and CD-4 expressions were observed in gingival tissues in Test group than in Control group. HN019 reduced the adhesion of P. gingivalis to BEC and showed antimicrobial potential against periodontopathogens. Conclusion Immunological and antimicrobial properties of B. lactis HN019 make it a potential probiotic to be used in non-surgical periodontal therapy of patients with GCP.Fil: Invernici, Marcos M.. Universidade de Sao Paulo; BrasilFil: Furlaneto, Flávia A. C.. Universidade de Sao Paulo; BrasilFil: Salvador, Sérgio L. Universidade de Sao Paulo; BrasilFil: Ouwehand, Arthur C.. Dupont, Nutrition and Health; FinlandiaFil: Salminen, Seppo. University of Turku. Functional Foods Forum; FinlandiaFil: Mantziari, Anastasia. University of Turku. Functional Foods Forum; FinlandiaFil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Ervolino, Edilson. São Paulo State University. Division of Histology, Department of Basic Sciences, Dental School of Araçatuba; BrasilFil: Santana, Sandro Isaías. Universidade de Sao Paulo; BrasilFil: Silva, Pedro Henrique Felix. Universidade de Sao Paulo; BrasilFil: Messora, Michel R.. Universidade de Sao Paulo; BrasilPublic Library of Science2020-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/117098Invernici, Marcos M.; Furlaneto, Flávia A. C.; Salvador, Sérgio L; Ouwehand, Arthur C.; Salminen, Seppo; et al.; Bifidobacterium animalis subsp lactis HN019 presents antimicrobial potential against periodontopathogens and modulates the immunological response of oral mucosa in periodontitis patients; Public Library of Science; Plos One; 15; 9; 9-2020; 1-20; e02384251932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0238425info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0238425info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:57Zoai:ri.conicet.gov.ar:11336/117098instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:57.85CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bifidobacterium animalis subsp lactis HN019 presents antimicrobial potential against periodontopathogens and modulates the immunological response of oral mucosa in periodontitis patients
title Bifidobacterium animalis subsp lactis HN019 presents antimicrobial potential against periodontopathogens and modulates the immunological response of oral mucosa in periodontitis patients
spellingShingle Bifidobacterium animalis subsp lactis HN019 presents antimicrobial potential against periodontopathogens and modulates the immunological response of oral mucosa in periodontitis patients
Invernici, Marcos M.
Bifidobacterium
Periodontal
Probiotics
title_short Bifidobacterium animalis subsp lactis HN019 presents antimicrobial potential against periodontopathogens and modulates the immunological response of oral mucosa in periodontitis patients
title_full Bifidobacterium animalis subsp lactis HN019 presents antimicrobial potential against periodontopathogens and modulates the immunological response of oral mucosa in periodontitis patients
title_fullStr Bifidobacterium animalis subsp lactis HN019 presents antimicrobial potential against periodontopathogens and modulates the immunological response of oral mucosa in periodontitis patients
title_full_unstemmed Bifidobacterium animalis subsp lactis HN019 presents antimicrobial potential against periodontopathogens and modulates the immunological response of oral mucosa in periodontitis patients
title_sort Bifidobacterium animalis subsp lactis HN019 presents antimicrobial potential against periodontopathogens and modulates the immunological response of oral mucosa in periodontitis patients
dc.creator.none.fl_str_mv Invernici, Marcos M.
Furlaneto, Flávia A. C.
Salvador, Sérgio L
Ouwehand, Arthur C.
Salminen, Seppo
Mantziari, Anastasia
Vinderola, Celso Gabriel
Ervolino, Edilson
Santana, Sandro Isaías
Silva, Pedro Henrique Felix
Messora, Michel R.
author Invernici, Marcos M.
author_facet Invernici, Marcos M.
Furlaneto, Flávia A. C.
Salvador, Sérgio L
Ouwehand, Arthur C.
Salminen, Seppo
Mantziari, Anastasia
Vinderola, Celso Gabriel
Ervolino, Edilson
Santana, Sandro Isaías
Silva, Pedro Henrique Felix
Messora, Michel R.
author_role author
author2 Furlaneto, Flávia A. C.
Salvador, Sérgio L
Ouwehand, Arthur C.
Salminen, Seppo
Mantziari, Anastasia
Vinderola, Celso Gabriel
Ervolino, Edilson
Santana, Sandro Isaías
Silva, Pedro Henrique Felix
Messora, Michel R.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bifidobacterium
Periodontal
Probiotics
topic Bifidobacterium
Periodontal
Probiotics
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Objective To evaluate the effects of Bifidobacterium animalis subsp. lactis HN019 (HN019) on clinical periodontal parameters (plaque accumulation and gingival bleeding), on immunocompetence of gingival tissues [expression of beta-defensin (BD)-3, toll-like receptor 4 (TLR4), cluster of differentiation(CD)-57 and CD-4], and on immunological properties of saliva (IgA levels) in non-surgical periodontal therapy in generalized chronic periodontitis (GCP) patients. Adhesion to buccal epithelial cells (BEC) and the antimicrobial properties of HN019 were also investigated. Materials and methods Thirty patients were recruited and monitored clinically at baseline (before scaling and root planing-SRP) and after 30 and 90 days. Patients were randomly assigned to Test (SRP +Probiotic, n = 15) or Control (SRP+Placebo, n = 15) group. Probiotic lozenges were used for 30 days. Gingival tissues and saliva were immunologically analyzed. The adhesion of HN019 with or without Porphyromonas gingivalis in BEC and its antimicrobial properties were investigated in in vitro assays. Data were statistically analyzed (p<0.05). Results Test group presented lower plaque index (30 days) and lower marginal gingival bleeding (90 days) when compared with Control group. Higher BD-3, TLR4 and CD-4 expressions were observed in gingival tissues in Test group than in Control group. HN019 reduced the adhesion of P. gingivalis to BEC and showed antimicrobial potential against periodontopathogens. Conclusion Immunological and antimicrobial properties of B. lactis HN019 make it a potential probiotic to be used in non-surgical periodontal therapy of patients with GCP.
Fil: Invernici, Marcos M.. Universidade de Sao Paulo; Brasil
Fil: Furlaneto, Flávia A. C.. Universidade de Sao Paulo; Brasil
Fil: Salvador, Sérgio L. Universidade de Sao Paulo; Brasil
Fil: Ouwehand, Arthur C.. Dupont, Nutrition and Health; Finlandia
Fil: Salminen, Seppo. University of Turku. Functional Foods Forum; Finlandia
Fil: Mantziari, Anastasia. University of Turku. Functional Foods Forum; Finlandia
Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Ervolino, Edilson. São Paulo State University. Division of Histology, Department of Basic Sciences, Dental School of Araçatuba; Brasil
Fil: Santana, Sandro Isaías. Universidade de Sao Paulo; Brasil
Fil: Silva, Pedro Henrique Felix. Universidade de Sao Paulo; Brasil
Fil: Messora, Michel R.. Universidade de Sao Paulo; Brasil
description Objective To evaluate the effects of Bifidobacterium animalis subsp. lactis HN019 (HN019) on clinical periodontal parameters (plaque accumulation and gingival bleeding), on immunocompetence of gingival tissues [expression of beta-defensin (BD)-3, toll-like receptor 4 (TLR4), cluster of differentiation(CD)-57 and CD-4], and on immunological properties of saliva (IgA levels) in non-surgical periodontal therapy in generalized chronic periodontitis (GCP) patients. Adhesion to buccal epithelial cells (BEC) and the antimicrobial properties of HN019 were also investigated. Materials and methods Thirty patients were recruited and monitored clinically at baseline (before scaling and root planing-SRP) and after 30 and 90 days. Patients were randomly assigned to Test (SRP +Probiotic, n = 15) or Control (SRP+Placebo, n = 15) group. Probiotic lozenges were used for 30 days. Gingival tissues and saliva were immunologically analyzed. The adhesion of HN019 with or without Porphyromonas gingivalis in BEC and its antimicrobial properties were investigated in in vitro assays. Data were statistically analyzed (p<0.05). Results Test group presented lower plaque index (30 days) and lower marginal gingival bleeding (90 days) when compared with Control group. Higher BD-3, TLR4 and CD-4 expressions were observed in gingival tissues in Test group than in Control group. HN019 reduced the adhesion of P. gingivalis to BEC and showed antimicrobial potential against periodontopathogens. Conclusion Immunological and antimicrobial properties of B. lactis HN019 make it a potential probiotic to be used in non-surgical periodontal therapy of patients with GCP.
publishDate 2020
dc.date.none.fl_str_mv 2020-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/117098
Invernici, Marcos M.; Furlaneto, Flávia A. C.; Salvador, Sérgio L; Ouwehand, Arthur C.; Salminen, Seppo; et al.; Bifidobacterium animalis subsp lactis HN019 presents antimicrobial potential against periodontopathogens and modulates the immunological response of oral mucosa in periodontitis patients; Public Library of Science; Plos One; 15; 9; 9-2020; 1-20; e0238425
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/117098
identifier_str_mv Invernici, Marcos M.; Furlaneto, Flávia A. C.; Salvador, Sérgio L; Ouwehand, Arthur C.; Salminen, Seppo; et al.; Bifidobacterium animalis subsp lactis HN019 presents antimicrobial potential against periodontopathogens and modulates the immunological response of oral mucosa in periodontitis patients; Public Library of Science; Plos One; 15; 9; 9-2020; 1-20; e0238425
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0238425
info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0238425
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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