Study of antimycobacterial, cytotoxic, and mutagenic potential of polymeric nanoparticles of copper (II) complex

Autores
Aleixo, Nadia Andrade; Gomes, Pietra Stefany da Silva; Silva, Patrícia Bento da; Sato, Mariana Rillo; Campos, Débora Leite; Barud, Hernane da Silva; Castro, Guillermo Raul; Islan, German Abel; Toledo, Constanza; Karp, Federico; Chorilli, Marlus; Pavan, Fernando Rogério; Resende, Flávia Aparecida
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study aimed to encapsulate and characterise a potential anti-tuberculosis copper complex (CuCl2(INH)2.H2O:I1) into polymeric nanoparticles (PNs) of polymethacrylate copolymers (Eudragit®, Eu) developed by nanoprecipitation method. NE30D, S100 and, E100 polymers were tested. The physicochemical characterisations were performed by DLS, TEM, FTIR, encapsulation efficiency and, in vitro release studies. Encapsulation of I1 in PN-NE30D, PN-E100, and PN-S100 was 26.3%, 94.5%, 22.6%, respectively. The particle size and zeta potentials were 82.3 nm and −24.5 mV for PNs-NE30D, 304.4 nm and +18.7 mV for PNs-E100, and 517.9 nm and −6.9 mV for PNs-S100, respectively. All PDIs were under 0.5. The formulations showed an I1 controlled release at alkaline pH with 29.7% from PNs-NE30D, 7.9% from PNs-E100 and, 28.1% from PNs-S100 at 1 h incubation. PNs were stable for at least 3 months. Particularly, PNs-NE30D demonstrated moderate inhibition of M. tuberculosis and low cytotoxic activity. None of the PNs induced mutagenicity.
Fil: Aleixo, Nadia Andrade. Universidad de Araraquara; Brasil
Fil: Gomes, Pietra Stefany da Silva. Universidad de Araraquara; Brasil
Fil: Silva, Patrícia Bento da. Universidade de Sao Paulo; Brasil. Nanobiotechnology Laboratory, Institute of Biological Sciences, Department of Genetics and Morphology, University of Brasilia; Brasil
Fil: Sato, Mariana Rillo. Universidade de Sao Paulo; Brasil. Department of Drugs and Medicines, School of Pharmaceutical Sciences, Sao Paulo State University (UNESP); Brasil
Fil: Campos, Débora Leite. Universidade de Sao Paulo; Brasil. Department of Biological Sciences, School of Pharmaceutical Sciences, Sao Paulo State University (UNESP); Brasil
Fil: Barud, Hernane da Silva. Universidad de Araraquara; Brasil
Fil: Castro, Guillermo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina. Max Planck Laboratory for Structural Biology, Chemistry and Molecular Biophysics of Rosario (MPLbioR, UNR-MPIbpC), Partner Laboratory of the Max Planck Institute for Biophysical Chemistry (MPIbpC, MPG), Universidad Nacional de Rosario, Centro de Estudios Interdisciplinarios (CEI), Rosario, Argentina; Argentina
Fil: Islan, German Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Toledo, Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Karp, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Chorilli, Marlus. Universidade de Sao Paulo; Brasil
Fil: Pavan, Fernando Rogério. Universidade de Sao Paulo; Brasil
Fil: Resende, Flávia Aparecida. Universidad de Araraquara; Brasil
Materia
COPPER(II) COMPLEXES
EUDRAGIT®
MUTAGENICITY
POLYMERIC MATRICES
RESAZURIN ASSAY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/203339
Acceder
id CONICETDig_871009b621f46b16b43b89a4596a92e9
oai_identifier_str oai:ri.conicet.gov.ar:11336/203339
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Study of antimycobacterial, cytotoxic, and mutagenic potential of polymeric nanoparticles of copper (II) complexAleixo, Nadia AndradeGomes, Pietra Stefany da SilvaSilva, Patrícia Bento daSato, Mariana RilloCampos, Débora LeiteBarud, Hernane da SilvaCastro, Guillermo RaulIslan, German AbelToledo, ConstanzaKarp, FedericoChorilli, MarlusPavan, Fernando RogérioResende, Flávia AparecidaCOPPER(II) COMPLEXESEUDRAGIT®MUTAGENICITYPOLYMERIC MATRICESRESAZURIN ASSAYhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2This study aimed to encapsulate and characterise a potential anti-tuberculosis copper complex (CuCl2(INH)2.H2O:I1) into polymeric nanoparticles (PNs) of polymethacrylate copolymers (Eudragit®, Eu) developed by nanoprecipitation method. NE30D, S100 and, E100 polymers were tested. The physicochemical characterisations were performed by DLS, TEM, FTIR, encapsulation efficiency and, in vitro release studies. Encapsulation of I1 in PN-NE30D, PN-E100, and PN-S100 was 26.3%, 94.5%, 22.6%, respectively. The particle size and zeta potentials were 82.3 nm and −24.5 mV for PNs-NE30D, 304.4 nm and +18.7 mV for PNs-E100, and 517.9 nm and −6.9 mV for PNs-S100, respectively. All PDIs were under 0.5. The formulations showed an I1 controlled release at alkaline pH with 29.7% from PNs-NE30D, 7.9% from PNs-E100 and, 28.1% from PNs-S100 at 1 h incubation. PNs were stable for at least 3 months. Particularly, PNs-NE30D demonstrated moderate inhibition of M. tuberculosis and low cytotoxic activity. None of the PNs induced mutagenicity.Fil: Aleixo, Nadia Andrade. Universidad de Araraquara; BrasilFil: Gomes, Pietra Stefany da Silva. Universidad de Araraquara; BrasilFil: Silva, Patrícia Bento da. Universidade de Sao Paulo; Brasil. Nanobiotechnology Laboratory, Institute of Biological Sciences, Department of Genetics and Morphology, University of Brasilia; BrasilFil: Sato, Mariana Rillo. Universidade de Sao Paulo; Brasil. Department of Drugs and Medicines, School of Pharmaceutical Sciences, Sao Paulo State University (UNESP); BrasilFil: Campos, Débora Leite. Universidade de Sao Paulo; Brasil. Department of Biological Sciences, School of Pharmaceutical Sciences, Sao Paulo State University (UNESP); BrasilFil: Barud, Hernane da Silva. Universidad de Araraquara; BrasilFil: Castro, Guillermo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina. Max Planck Laboratory for Structural Biology, Chemistry and Molecular Biophysics of Rosario (MPLbioR, UNR-MPIbpC), Partner Laboratory of the Max Planck Institute for Biophysical Chemistry (MPIbpC, MPG), Universidad Nacional de Rosario, Centro de Estudios Interdisciplinarios (CEI), Rosario, Argentina; ArgentinaFil: Islan, German Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Toledo, Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Karp, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Chorilli, Marlus. Universidade de Sao Paulo; BrasilFil: Pavan, Fernando Rogério. Universidade de Sao Paulo; BrasilFil: Resende, Flávia Aparecida. Universidad de Araraquara; BrasilTaylor & Francis Ltd2022-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/203339Aleixo, Nadia Andrade; Gomes, Pietra Stefany da Silva; Silva, Patrícia Bento da; Sato, Mariana Rillo; Campos, Débora Leite; et al.; Study of antimycobacterial, cytotoxic, and mutagenic potential of polymeric nanoparticles of copper (II) complex; Taylor & Francis Ltd; Journal Of Microencapsulation; 39; 1; 1-2022; 61-710265-2048CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/02652048.2022.2025935info:eu-repo/semantics/altIdentifier/doi/10.1080/02652048.2022.2025935info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:25:40Zoai:ri.conicet.gov.ar:11336/203339instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:25:40.475CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Study of antimycobacterial, cytotoxic, and mutagenic potential of polymeric nanoparticles of copper (II) complex
title Study of antimycobacterial, cytotoxic, and mutagenic potential of polymeric nanoparticles of copper (II) complex
spellingShingle Study of antimycobacterial, cytotoxic, and mutagenic potential of polymeric nanoparticles of copper (II) complex
Aleixo, Nadia Andrade
COPPER(II) COMPLEXES
EUDRAGIT®
MUTAGENICITY
POLYMERIC MATRICES
RESAZURIN ASSAY
title_short Study of antimycobacterial, cytotoxic, and mutagenic potential of polymeric nanoparticles of copper (II) complex
title_full Study of antimycobacterial, cytotoxic, and mutagenic potential of polymeric nanoparticles of copper (II) complex
title_fullStr Study of antimycobacterial, cytotoxic, and mutagenic potential of polymeric nanoparticles of copper (II) complex
title_full_unstemmed Study of antimycobacterial, cytotoxic, and mutagenic potential of polymeric nanoparticles of copper (II) complex
title_sort Study of antimycobacterial, cytotoxic, and mutagenic potential of polymeric nanoparticles of copper (II) complex
dc.creator.none.fl_str_mv Aleixo, Nadia Andrade
Gomes, Pietra Stefany da Silva
Silva, Patrícia Bento da
Sato, Mariana Rillo
Campos, Débora Leite
Barud, Hernane da Silva
Castro, Guillermo Raul
Islan, German Abel
Toledo, Constanza
Karp, Federico
Chorilli, Marlus
Pavan, Fernando Rogério
Resende, Flávia Aparecida
author Aleixo, Nadia Andrade
author_facet Aleixo, Nadia Andrade
Gomes, Pietra Stefany da Silva
Silva, Patrícia Bento da
Sato, Mariana Rillo
Campos, Débora Leite
Barud, Hernane da Silva
Castro, Guillermo Raul
Islan, German Abel
Toledo, Constanza
Karp, Federico
Chorilli, Marlus
Pavan, Fernando Rogério
Resende, Flávia Aparecida
author_role author
author2 Gomes, Pietra Stefany da Silva
Silva, Patrícia Bento da
Sato, Mariana Rillo
Campos, Débora Leite
Barud, Hernane da Silva
Castro, Guillermo Raul
Islan, German Abel
Toledo, Constanza
Karp, Federico
Chorilli, Marlus
Pavan, Fernando Rogério
Resende, Flávia Aparecida
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv COPPER(II) COMPLEXES
EUDRAGIT®
MUTAGENICITY
POLYMERIC MATRICES
RESAZURIN ASSAY
topic COPPER(II) COMPLEXES
EUDRAGIT®
MUTAGENICITY
POLYMERIC MATRICES
RESAZURIN ASSAY
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv This study aimed to encapsulate and characterise a potential anti-tuberculosis copper complex (CuCl2(INH)2.H2O:I1) into polymeric nanoparticles (PNs) of polymethacrylate copolymers (Eudragit®, Eu) developed by nanoprecipitation method. NE30D, S100 and, E100 polymers were tested. The physicochemical characterisations were performed by DLS, TEM, FTIR, encapsulation efficiency and, in vitro release studies. Encapsulation of I1 in PN-NE30D, PN-E100, and PN-S100 was 26.3%, 94.5%, 22.6%, respectively. The particle size and zeta potentials were 82.3 nm and −24.5 mV for PNs-NE30D, 304.4 nm and +18.7 mV for PNs-E100, and 517.9 nm and −6.9 mV for PNs-S100, respectively. All PDIs were under 0.5. The formulations showed an I1 controlled release at alkaline pH with 29.7% from PNs-NE30D, 7.9% from PNs-E100 and, 28.1% from PNs-S100 at 1 h incubation. PNs were stable for at least 3 months. Particularly, PNs-NE30D demonstrated moderate inhibition of M. tuberculosis and low cytotoxic activity. None of the PNs induced mutagenicity.
Fil: Aleixo, Nadia Andrade. Universidad de Araraquara; Brasil
Fil: Gomes, Pietra Stefany da Silva. Universidad de Araraquara; Brasil
Fil: Silva, Patrícia Bento da. Universidade de Sao Paulo; Brasil. Nanobiotechnology Laboratory, Institute of Biological Sciences, Department of Genetics and Morphology, University of Brasilia; Brasil
Fil: Sato, Mariana Rillo. Universidade de Sao Paulo; Brasil. Department of Drugs and Medicines, School of Pharmaceutical Sciences, Sao Paulo State University (UNESP); Brasil
Fil: Campos, Débora Leite. Universidade de Sao Paulo; Brasil. Department of Biological Sciences, School of Pharmaceutical Sciences, Sao Paulo State University (UNESP); Brasil
Fil: Barud, Hernane da Silva. Universidad de Araraquara; Brasil
Fil: Castro, Guillermo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina. Max Planck Laboratory for Structural Biology, Chemistry and Molecular Biophysics of Rosario (MPLbioR, UNR-MPIbpC), Partner Laboratory of the Max Planck Institute for Biophysical Chemistry (MPIbpC, MPG), Universidad Nacional de Rosario, Centro de Estudios Interdisciplinarios (CEI), Rosario, Argentina; Argentina
Fil: Islan, German Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Toledo, Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Karp, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Chorilli, Marlus. Universidade de Sao Paulo; Brasil
Fil: Pavan, Fernando Rogério. Universidade de Sao Paulo; Brasil
Fil: Resende, Flávia Aparecida. Universidad de Araraquara; Brasil
description This study aimed to encapsulate and characterise a potential anti-tuberculosis copper complex (CuCl2(INH)2.H2O:I1) into polymeric nanoparticles (PNs) of polymethacrylate copolymers (Eudragit®, Eu) developed by nanoprecipitation method. NE30D, S100 and, E100 polymers were tested. The physicochemical characterisations were performed by DLS, TEM, FTIR, encapsulation efficiency and, in vitro release studies. Encapsulation of I1 in PN-NE30D, PN-E100, and PN-S100 was 26.3%, 94.5%, 22.6%, respectively. The particle size and zeta potentials were 82.3 nm and −24.5 mV for PNs-NE30D, 304.4 nm and +18.7 mV for PNs-E100, and 517.9 nm and −6.9 mV for PNs-S100, respectively. All PDIs were under 0.5. The formulations showed an I1 controlled release at alkaline pH with 29.7% from PNs-NE30D, 7.9% from PNs-E100 and, 28.1% from PNs-S100 at 1 h incubation. PNs were stable for at least 3 months. Particularly, PNs-NE30D demonstrated moderate inhibition of M. tuberculosis and low cytotoxic activity. None of the PNs induced mutagenicity.
publishDate 2022
dc.date.none.fl_str_mv 2022-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/203339
Aleixo, Nadia Andrade; Gomes, Pietra Stefany da Silva; Silva, Patrícia Bento da; Sato, Mariana Rillo; Campos, Débora Leite; et al.; Study of antimycobacterial, cytotoxic, and mutagenic potential of polymeric nanoparticles of copper (II) complex; Taylor & Francis Ltd; Journal Of Microencapsulation; 39; 1; 1-2022; 61-71
0265-2048
CONICET Digital
CONICET
url http://hdl.handle.net/11336/203339
identifier_str_mv Aleixo, Nadia Andrade; Gomes, Pietra Stefany da Silva; Silva, Patrícia Bento da; Sato, Mariana Rillo; Campos, Débora Leite; et al.; Study of antimycobacterial, cytotoxic, and mutagenic potential of polymeric nanoparticles of copper (II) complex; Taylor & Francis Ltd; Journal Of Microencapsulation; 39; 1; 1-2022; 61-71
0265-2048
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/02652048.2022.2025935
info:eu-repo/semantics/altIdentifier/doi/10.1080/02652048.2022.2025935
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis Ltd
publisher.none.fl_str_mv Taylor & Francis Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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