Multicentre laboratory validation of the colorimetric redox indicator (CRI) assay for the rapid detection of extensively drug-resistant (XDR) Mycobacterium tuberculosis

Autores
Martin, Anandi; Paasch, Fabienne; Docx, Sven; Fissette, Krista; Imperiale, Belén Rocío; Ribón, Wellman; González, Liliana Andrea; Werngren, Jim; Engström, Anna; Skenders, Girts; Juréen, Pontus; Hoffner, Sven; Del Portillo, Patricia; Morcillo, Nora Susana; Palomino, Juan Carlos
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objectives: To perform a multicentre study to evaluate the performance of the colorimetric redox indicator (CRI) assay and to establish the MICs and critical concentrations of rifampicin, isoniazid, ofloxacin, kanamycin and capreomycin. Methods: The study was carried out in two phases. Phase I determined the MIC of each drug. Phase II established critical concentrations for the five drugs tested by the CRI assay compared with the conventional proportion method. Results: Phase I: a strain was considered resistant by the CRI assay if the MIC was 0.5 mg/L for rifampicin, 0.25 mg/L for isoniazid, 4.0 mg/L for ofloxacin and 5.0 mg/L for kanamycin and capreomycin. Sensitivity was 99.1% for isoniazid and 100% for the other drugs and specificity was 97.9% for capreomycin and 100% for the other drugs. Phase II: the critical concentration was 0.5 mg/L for rifampicin, 0.25 mg/L for isoniazid, 2.0 mg/L for ofloxacin and 2.5 mg/L for kanamycin and capreomycin giving an overall accuracy of 98.4%, 96.6%, 96.7%, 98.3% and 90%, respectively. Conclusions: Results demonstrate that the CRI assay is an accurate method for the rapid detection of XDR Mycobacterium tuberculosis. The CRI assay is faster than the conventional drug susceptibility testing method using solid medium, has the same turnaround time as the BACTEC MGIT 960 system, but is less expensive, and could be an adequate method for low-income countries.
Fil: Martin, Anandi. Institute of Tropical Medicine; Bélgica
Fil: Paasch, Fabienne. Institute of Tropical Medicine; Bélgica
Fil: Docx, Sven. Institute of Tropical Medicine; Bélgica
Fil: Fissette, Krista. Institute of Tropical Medicine; Bélgica
Fil: Imperiale, Belén Rocío. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ribón, Wellman. Universidad Industrial Santander; Colombia
Fil: González, Liliana Andrea. Universidad Industrial Santander; Colombia
Fil: Werngren, Jim. Swedish Institute for Infectious Disease Control; Suecia
Fil: Engström, Anna. Swedish Institute for Infectious Disease Control; Suecia
Fil: Skenders, Girts. No especifíca;
Fil: Juréen, Pontus. Swedish Institute for Infectious Disease Control; Suecia
Fil: Hoffner, Sven. Swedish Institute for Infectious Disease Control; Suecia
Fil: Del Portillo, Patricia. No especifíca;
Fil: Morcillo, Nora Susana. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Palomino, Juan Carlos. Institute of Tropical Medicine; Bélgica
Materia
DRUG RESISTANCE
RESAZURIN
SECOND-LINE DRUGS
TUBERCULOSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/192105
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/192105
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Multicentre laboratory validation of the colorimetric redox indicator (CRI) assay for the rapid detection of extensively drug-resistant (XDR) Mycobacterium tuberculosisMartin, AnandiPaasch, FabienneDocx, SvenFissette, KristaImperiale, Belén RocíoRibón, WellmanGonzález, Liliana AndreaWerngren, JimEngström, AnnaSkenders, GirtsJuréen, PontusHoffner, SvenDel Portillo, PatriciaMorcillo, Nora SusanaPalomino, Juan CarlosDRUG RESISTANCERESAZURINSECOND-LINE DRUGSTUBERCULOSIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Objectives: To perform a multicentre study to evaluate the performance of the colorimetric redox indicator (CRI) assay and to establish the MICs and critical concentrations of rifampicin, isoniazid, ofloxacin, kanamycin and capreomycin. Methods: The study was carried out in two phases. Phase I determined the MIC of each drug. Phase II established critical concentrations for the five drugs tested by the CRI assay compared with the conventional proportion method. Results: Phase I: a strain was considered resistant by the CRI assay if the MIC was 0.5 mg/L for rifampicin, 0.25 mg/L for isoniazid, 4.0 mg/L for ofloxacin and 5.0 mg/L for kanamycin and capreomycin. Sensitivity was 99.1% for isoniazid and 100% for the other drugs and specificity was 97.9% for capreomycin and 100% for the other drugs. Phase II: the critical concentration was 0.5 mg/L for rifampicin, 0.25 mg/L for isoniazid, 2.0 mg/L for ofloxacin and 2.5 mg/L for kanamycin and capreomycin giving an overall accuracy of 98.4%, 96.6%, 96.7%, 98.3% and 90%, respectively. Conclusions: Results demonstrate that the CRI assay is an accurate method for the rapid detection of XDR Mycobacterium tuberculosis. The CRI assay is faster than the conventional drug susceptibility testing method using solid medium, has the same turnaround time as the BACTEC MGIT 960 system, but is less expensive, and could be an adequate method for low-income countries.Fil: Martin, Anandi. Institute of Tropical Medicine; BélgicaFil: Paasch, Fabienne. Institute of Tropical Medicine; BélgicaFil: Docx, Sven. Institute of Tropical Medicine; BélgicaFil: Fissette, Krista. Institute of Tropical Medicine; BélgicaFil: Imperiale, Belén Rocío. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ribón, Wellman. Universidad Industrial Santander; ColombiaFil: González, Liliana Andrea. Universidad Industrial Santander; ColombiaFil: Werngren, Jim. Swedish Institute for Infectious Disease Control; SueciaFil: Engström, Anna. Swedish Institute for Infectious Disease Control; SueciaFil: Skenders, Girts. No especifíca;Fil: Juréen, Pontus. Swedish Institute for Infectious Disease Control; SueciaFil: Hoffner, Sven. Swedish Institute for Infectious Disease Control; SueciaFil: Del Portillo, Patricia. No especifíca;Fil: Morcillo, Nora Susana. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; ArgentinaFil: Palomino, Juan Carlos. Institute of Tropical Medicine; BélgicaOxford University Press2011-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/192105Martin, Anandi; Paasch, Fabienne; Docx, Sven; Fissette, Krista; Imperiale, Belén Rocío; et al.; Multicentre laboratory validation of the colorimetric redox indicator (CRI) assay for the rapid detection of extensively drug-resistant (XDR) Mycobacterium tuberculosis; Oxford University Press; Journal of Antimicrobial Chemotherapy; 66; 4; 4-2011; 827-8330305-7453CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jac/article/66/4/827/723957info:eu-repo/semantics/altIdentifier/doi/10.1093/jac/dkq527info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:06:08Zoai:ri.conicet.gov.ar:11336/192105instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:06:08.747CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Multicentre laboratory validation of the colorimetric redox indicator (CRI) assay for the rapid detection of extensively drug-resistant (XDR) Mycobacterium tuberculosis
title Multicentre laboratory validation of the colorimetric redox indicator (CRI) assay for the rapid detection of extensively drug-resistant (XDR) Mycobacterium tuberculosis
spellingShingle Multicentre laboratory validation of the colorimetric redox indicator (CRI) assay for the rapid detection of extensively drug-resistant (XDR) Mycobacterium tuberculosis
Martin, Anandi
DRUG RESISTANCE
RESAZURIN
SECOND-LINE DRUGS
TUBERCULOSIS
title_short Multicentre laboratory validation of the colorimetric redox indicator (CRI) assay for the rapid detection of extensively drug-resistant (XDR) Mycobacterium tuberculosis
title_full Multicentre laboratory validation of the colorimetric redox indicator (CRI) assay for the rapid detection of extensively drug-resistant (XDR) Mycobacterium tuberculosis
title_fullStr Multicentre laboratory validation of the colorimetric redox indicator (CRI) assay for the rapid detection of extensively drug-resistant (XDR) Mycobacterium tuberculosis
title_full_unstemmed Multicentre laboratory validation of the colorimetric redox indicator (CRI) assay for the rapid detection of extensively drug-resistant (XDR) Mycobacterium tuberculosis
title_sort Multicentre laboratory validation of the colorimetric redox indicator (CRI) assay for the rapid detection of extensively drug-resistant (XDR) Mycobacterium tuberculosis
dc.creator.none.fl_str_mv Martin, Anandi
Paasch, Fabienne
Docx, Sven
Fissette, Krista
Imperiale, Belén Rocío
Ribón, Wellman
González, Liliana Andrea
Werngren, Jim
Engström, Anna
Skenders, Girts
Juréen, Pontus
Hoffner, Sven
Del Portillo, Patricia
Morcillo, Nora Susana
Palomino, Juan Carlos
author Martin, Anandi
author_facet Martin, Anandi
Paasch, Fabienne
Docx, Sven
Fissette, Krista
Imperiale, Belén Rocío
Ribón, Wellman
González, Liliana Andrea
Werngren, Jim
Engström, Anna
Skenders, Girts
Juréen, Pontus
Hoffner, Sven
Del Portillo, Patricia
Morcillo, Nora Susana
Palomino, Juan Carlos
author_role author
author2 Paasch, Fabienne
Docx, Sven
Fissette, Krista
Imperiale, Belén Rocío
Ribón, Wellman
González, Liliana Andrea
Werngren, Jim
Engström, Anna
Skenders, Girts
Juréen, Pontus
Hoffner, Sven
Del Portillo, Patricia
Morcillo, Nora Susana
Palomino, Juan Carlos
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DRUG RESISTANCE
RESAZURIN
SECOND-LINE DRUGS
TUBERCULOSIS
topic DRUG RESISTANCE
RESAZURIN
SECOND-LINE DRUGS
TUBERCULOSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Objectives: To perform a multicentre study to evaluate the performance of the colorimetric redox indicator (CRI) assay and to establish the MICs and critical concentrations of rifampicin, isoniazid, ofloxacin, kanamycin and capreomycin. Methods: The study was carried out in two phases. Phase I determined the MIC of each drug. Phase II established critical concentrations for the five drugs tested by the CRI assay compared with the conventional proportion method. Results: Phase I: a strain was considered resistant by the CRI assay if the MIC was 0.5 mg/L for rifampicin, 0.25 mg/L for isoniazid, 4.0 mg/L for ofloxacin and 5.0 mg/L for kanamycin and capreomycin. Sensitivity was 99.1% for isoniazid and 100% for the other drugs and specificity was 97.9% for capreomycin and 100% for the other drugs. Phase II: the critical concentration was 0.5 mg/L for rifampicin, 0.25 mg/L for isoniazid, 2.0 mg/L for ofloxacin and 2.5 mg/L for kanamycin and capreomycin giving an overall accuracy of 98.4%, 96.6%, 96.7%, 98.3% and 90%, respectively. Conclusions: Results demonstrate that the CRI assay is an accurate method for the rapid detection of XDR Mycobacterium tuberculosis. The CRI assay is faster than the conventional drug susceptibility testing method using solid medium, has the same turnaround time as the BACTEC MGIT 960 system, but is less expensive, and could be an adequate method for low-income countries.
Fil: Martin, Anandi. Institute of Tropical Medicine; Bélgica
Fil: Paasch, Fabienne. Institute of Tropical Medicine; Bélgica
Fil: Docx, Sven. Institute of Tropical Medicine; Bélgica
Fil: Fissette, Krista. Institute of Tropical Medicine; Bélgica
Fil: Imperiale, Belén Rocío. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ribón, Wellman. Universidad Industrial Santander; Colombia
Fil: González, Liliana Andrea. Universidad Industrial Santander; Colombia
Fil: Werngren, Jim. Swedish Institute for Infectious Disease Control; Suecia
Fil: Engström, Anna. Swedish Institute for Infectious Disease Control; Suecia
Fil: Skenders, Girts. No especifíca;
Fil: Juréen, Pontus. Swedish Institute for Infectious Disease Control; Suecia
Fil: Hoffner, Sven. Swedish Institute for Infectious Disease Control; Suecia
Fil: Del Portillo, Patricia. No especifíca;
Fil: Morcillo, Nora Susana. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Palomino, Juan Carlos. Institute of Tropical Medicine; Bélgica
description Objectives: To perform a multicentre study to evaluate the performance of the colorimetric redox indicator (CRI) assay and to establish the MICs and critical concentrations of rifampicin, isoniazid, ofloxacin, kanamycin and capreomycin. Methods: The study was carried out in two phases. Phase I determined the MIC of each drug. Phase II established critical concentrations for the five drugs tested by the CRI assay compared with the conventional proportion method. Results: Phase I: a strain was considered resistant by the CRI assay if the MIC was 0.5 mg/L for rifampicin, 0.25 mg/L for isoniazid, 4.0 mg/L for ofloxacin and 5.0 mg/L for kanamycin and capreomycin. Sensitivity was 99.1% for isoniazid and 100% for the other drugs and specificity was 97.9% for capreomycin and 100% for the other drugs. Phase II: the critical concentration was 0.5 mg/L for rifampicin, 0.25 mg/L for isoniazid, 2.0 mg/L for ofloxacin and 2.5 mg/L for kanamycin and capreomycin giving an overall accuracy of 98.4%, 96.6%, 96.7%, 98.3% and 90%, respectively. Conclusions: Results demonstrate that the CRI assay is an accurate method for the rapid detection of XDR Mycobacterium tuberculosis. The CRI assay is faster than the conventional drug susceptibility testing method using solid medium, has the same turnaround time as the BACTEC MGIT 960 system, but is less expensive, and could be an adequate method for low-income countries.
publishDate 2011
dc.date.none.fl_str_mv 2011-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/192105
Martin, Anandi; Paasch, Fabienne; Docx, Sven; Fissette, Krista; Imperiale, Belén Rocío; et al.; Multicentre laboratory validation of the colorimetric redox indicator (CRI) assay for the rapid detection of extensively drug-resistant (XDR) Mycobacterium tuberculosis; Oxford University Press; Journal of Antimicrobial Chemotherapy; 66; 4; 4-2011; 827-833
0305-7453
CONICET Digital
CONICET
url http://hdl.handle.net/11336/192105
identifier_str_mv Martin, Anandi; Paasch, Fabienne; Docx, Sven; Fissette, Krista; Imperiale, Belén Rocío; et al.; Multicentre laboratory validation of the colorimetric redox indicator (CRI) assay for the rapid detection of extensively drug-resistant (XDR) Mycobacterium tuberculosis; Oxford University Press; Journal of Antimicrobial Chemotherapy; 66; 4; 4-2011; 827-833
0305-7453
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jac/article/66/4/827/723957
info:eu-repo/semantics/altIdentifier/doi/10.1093/jac/dkq527
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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