Photodynamic Effects of Zinc(II) Phthalocyanine-Loaded Polymeric Micelles in Human Nasopharynx KB Carcinoma Cells

Autores
Garcia Vior, María Cecilia; Marino, Julieta; Roguin, Leonor Patricia; Sosnik, Alejandro Dario; Awruch, Josefina
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A major difficulty in photodynamic therapy is the poor solubility of the photosensitizer (PS) under physiological conditions which correlates with low bioavailability. PS aggregation leads to a decrease in the photodynamic efficiency and a more limited activity in vitro and in vivo. To improve the aqueous solubility and reduce the aggregation of 2,9(10),16(17),23(24)-tetrakis[(2-dimethylamino)ethylsulfanyl] phthal-ocyaninatozinc(II) (Pc9), the encapsulation into four poloxamine polymeric micelles (T304, T904, T1107 and T1307) displaying a broad spectrum of molecular weight and hydrophilic–lipophilic balance was investigated. The aqueous solubility of Pc9 was increased up to 30 times. Morphological evaluation showed the formation of Pc9-loaded spherical micelles in the nanosize range. UV/Vis and fluorescence studies indicated that Pc9 is less aggregated upon encapsulation in comparison with Pc9 in water–DMSO 2% and remained photostable. Pc9-loaded micelles generated singlet molecular oxygen in high yields. Photocytotoxicity assays using human nasopharynx KB carcinoma cells confirmed that the encapsulation of Pc9 in T1107 and T1307 increases its photocytotoxicity by 10 times in comparison with the free form in water–DMSO. In addition, Pc9 incorporated into cells was mainly localized in lysosomes.
Fil: Garcia Vior, María Cecilia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Orgánica; Argentina;
Fil: Marino, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina;
Fil: Roguin, Leonor Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina;
Fil: Sosnik, Alejandro Dario. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina;
Fil: Awruch, Josefina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Orgánica; Argentina;
Materia
Photodynamic Therapy
Zinc(Ii) Phthalocyanine
Poloxamine Polymeric Micelles
Photocytotoxicity Assay
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/1722
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/1722
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Photodynamic Effects of Zinc(II) Phthalocyanine-Loaded Polymeric Micelles in Human Nasopharynx KB Carcinoma CellsGarcia Vior, María CeciliaMarino, JulietaRoguin, Leonor PatriciaSosnik, Alejandro DarioAwruch, JosefinaPhotodynamic TherapyZinc(Ii) PhthalocyaninePoloxamine Polymeric MicellesPhotocytotoxicity Assayhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3A major difficulty in photodynamic therapy is the poor solubility of the photosensitizer (PS) under physiological conditions which correlates with low bioavailability. PS aggregation leads to a decrease in the photodynamic efficiency and a more limited activity in vitro and in vivo. To improve the aqueous solubility and reduce the aggregation of 2,9(10),16(17),23(24)-tetrakis[(2-dimethylamino)ethylsulfanyl] phthal-ocyaninatozinc(II) (Pc9), the encapsulation into four poloxamine polymeric micelles (T304, T904, T1107 and T1307) displaying a broad spectrum of molecular weight and hydrophilic–lipophilic balance was investigated. The aqueous solubility of Pc9 was increased up to 30 times. Morphological evaluation showed the formation of Pc9-loaded spherical micelles in the nanosize range. UV/Vis and fluorescence studies indicated that Pc9 is less aggregated upon encapsulation in comparison with Pc9 in water–DMSO 2% and remained photostable. Pc9-loaded micelles generated singlet molecular oxygen in high yields. Photocytotoxicity assays using human nasopharynx KB carcinoma cells confirmed that the encapsulation of Pc9 in T1107 and T1307 increases its photocytotoxicity by 10 times in comparison with the free form in water–DMSO. In addition, Pc9 incorporated into cells was mainly localized in lysosomes.Fil: Garcia Vior, María Cecilia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Orgánica; Argentina;Fil: Marino, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina;Fil: Roguin, Leonor Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina;Fil: Sosnik, Alejandro Dario. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina;Fil: Awruch, Josefina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Orgánica; Argentina;Wiley2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1722Garcia Vior, María Cecilia; Marino, Julieta; Roguin, Leonor Patricia; Sosnik, Alejandro Dario; Awruch, Josefina; Photodynamic Effects of Zinc(II) Phthalocyanine-Loaded Polymeric Micelles in Human Nasopharynx KB Carcinoma Cells; Wiley; Photochemistry and Photobiology; 89; 2; 3-2013; 429-5000031-8655enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1751-1097.2012.01229.x/abstractinfo:eu-repo/semantics/altIdentifier/doi/doi:10.1111/j.1751-1097.2012.01229.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:02:32Zoai:ri.conicet.gov.ar:11336/1722instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:02:33.184CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Photodynamic Effects of Zinc(II) Phthalocyanine-Loaded Polymeric Micelles in Human Nasopharynx KB Carcinoma Cells
title Photodynamic Effects of Zinc(II) Phthalocyanine-Loaded Polymeric Micelles in Human Nasopharynx KB Carcinoma Cells
spellingShingle Photodynamic Effects of Zinc(II) Phthalocyanine-Loaded Polymeric Micelles in Human Nasopharynx KB Carcinoma Cells
Garcia Vior, María Cecilia
Photodynamic Therapy
Zinc(Ii) Phthalocyanine
Poloxamine Polymeric Micelles
Photocytotoxicity Assay
title_short Photodynamic Effects of Zinc(II) Phthalocyanine-Loaded Polymeric Micelles in Human Nasopharynx KB Carcinoma Cells
title_full Photodynamic Effects of Zinc(II) Phthalocyanine-Loaded Polymeric Micelles in Human Nasopharynx KB Carcinoma Cells
title_fullStr Photodynamic Effects of Zinc(II) Phthalocyanine-Loaded Polymeric Micelles in Human Nasopharynx KB Carcinoma Cells
title_full_unstemmed Photodynamic Effects of Zinc(II) Phthalocyanine-Loaded Polymeric Micelles in Human Nasopharynx KB Carcinoma Cells
title_sort Photodynamic Effects of Zinc(II) Phthalocyanine-Loaded Polymeric Micelles in Human Nasopharynx KB Carcinoma Cells
dc.creator.none.fl_str_mv Garcia Vior, María Cecilia
Marino, Julieta
Roguin, Leonor Patricia
Sosnik, Alejandro Dario
Awruch, Josefina
author Garcia Vior, María Cecilia
author_facet Garcia Vior, María Cecilia
Marino, Julieta
Roguin, Leonor Patricia
Sosnik, Alejandro Dario
Awruch, Josefina
author_role author
author2 Marino, Julieta
Roguin, Leonor Patricia
Sosnik, Alejandro Dario
Awruch, Josefina
author2_role author
author
author
author
dc.subject.none.fl_str_mv Photodynamic Therapy
Zinc(Ii) Phthalocyanine
Poloxamine Polymeric Micelles
Photocytotoxicity Assay
topic Photodynamic Therapy
Zinc(Ii) Phthalocyanine
Poloxamine Polymeric Micelles
Photocytotoxicity Assay
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv A major difficulty in photodynamic therapy is the poor solubility of the photosensitizer (PS) under physiological conditions which correlates with low bioavailability. PS aggregation leads to a decrease in the photodynamic efficiency and a more limited activity in vitro and in vivo. To improve the aqueous solubility and reduce the aggregation of 2,9(10),16(17),23(24)-tetrakis[(2-dimethylamino)ethylsulfanyl] phthal-ocyaninatozinc(II) (Pc9), the encapsulation into four poloxamine polymeric micelles (T304, T904, T1107 and T1307) displaying a broad spectrum of molecular weight and hydrophilic–lipophilic balance was investigated. The aqueous solubility of Pc9 was increased up to 30 times. Morphological evaluation showed the formation of Pc9-loaded spherical micelles in the nanosize range. UV/Vis and fluorescence studies indicated that Pc9 is less aggregated upon encapsulation in comparison with Pc9 in water–DMSO 2% and remained photostable. Pc9-loaded micelles generated singlet molecular oxygen in high yields. Photocytotoxicity assays using human nasopharynx KB carcinoma cells confirmed that the encapsulation of Pc9 in T1107 and T1307 increases its photocytotoxicity by 10 times in comparison with the free form in water–DMSO. In addition, Pc9 incorporated into cells was mainly localized in lysosomes.
Fil: Garcia Vior, María Cecilia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Orgánica; Argentina;
Fil: Marino, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina;
Fil: Roguin, Leonor Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina;
Fil: Sosnik, Alejandro Dario. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina;
Fil: Awruch, Josefina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Orgánica; Argentina;
description A major difficulty in photodynamic therapy is the poor solubility of the photosensitizer (PS) under physiological conditions which correlates with low bioavailability. PS aggregation leads to a decrease in the photodynamic efficiency and a more limited activity in vitro and in vivo. To improve the aqueous solubility and reduce the aggregation of 2,9(10),16(17),23(24)-tetrakis[(2-dimethylamino)ethylsulfanyl] phthal-ocyaninatozinc(II) (Pc9), the encapsulation into four poloxamine polymeric micelles (T304, T904, T1107 and T1307) displaying a broad spectrum of molecular weight and hydrophilic–lipophilic balance was investigated. The aqueous solubility of Pc9 was increased up to 30 times. Morphological evaluation showed the formation of Pc9-loaded spherical micelles in the nanosize range. UV/Vis and fluorescence studies indicated that Pc9 is less aggregated upon encapsulation in comparison with Pc9 in water–DMSO 2% and remained photostable. Pc9-loaded micelles generated singlet molecular oxygen in high yields. Photocytotoxicity assays using human nasopharynx KB carcinoma cells confirmed that the encapsulation of Pc9 in T1107 and T1307 increases its photocytotoxicity by 10 times in comparison with the free form in water–DMSO. In addition, Pc9 incorporated into cells was mainly localized in lysosomes.
publishDate 2013
dc.date.none.fl_str_mv 2013-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/1722
Garcia Vior, María Cecilia; Marino, Julieta; Roguin, Leonor Patricia; Sosnik, Alejandro Dario; Awruch, Josefina; Photodynamic Effects of Zinc(II) Phthalocyanine-Loaded Polymeric Micelles in Human Nasopharynx KB Carcinoma Cells; Wiley; Photochemistry and Photobiology; 89; 2; 3-2013; 429-500
0031-8655
url http://hdl.handle.net/11336/1722
identifier_str_mv Garcia Vior, María Cecilia; Marino, Julieta; Roguin, Leonor Patricia; Sosnik, Alejandro Dario; Awruch, Josefina; Photodynamic Effects of Zinc(II) Phthalocyanine-Loaded Polymeric Micelles in Human Nasopharynx KB Carcinoma Cells; Wiley; Photochemistry and Photobiology; 89; 2; 3-2013; 429-500
0031-8655
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1751-1097.2012.01229.x/abstract
info:eu-repo/semantics/altIdentifier/doi/doi:10.1111/j.1751-1097.2012.01229.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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