A friendly method for Raphanus sativus L (wild radish) peroxidase purification by polyelectrolyte precipitation

Autores
Woitovich Valetti, Nadia; Picó, Guillermo Alfredo
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The separation of radish peroxidase from a fresh Raphanus sativus L extract was carried out using precipitation with two commercially available negatively charged synthetic polyelectrolytes: Eudragit® L 100 and Eudragit® S 100. The enzyme was precipitated by polyelectrolyte addition at pH 4.00. The non-soluble complex formed was separated by simple centrifugation and re-dissolved by a pH change. The recovery of radish peroxidase biological activity was 50% of the initial activity in the homogenate for EuL and 45% for EuS, with 1.5-fold increase in its specific activity. The total Eudragit® concentration to precipitate the enzyme was very low: about 2 × 10 -3% w/v. The volume of the final product decreased to 10% of the feedstock, concentrating the sample up to 10 times.
Fil: Woitovich Valetti, Nadia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; Argentina
Fil: Picó, Guillermo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; Argentina
Materia
EUDRAGIT L 100
EUDRAGIT S 100
PEROXIDASE
POLYELECTROLYTES
PURIFICATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/94353
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/94353
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A friendly method for Raphanus sativus L (wild radish) peroxidase purification by polyelectrolyte precipitationWoitovich Valetti, NadiaPicó, Guillermo AlfredoEUDRAGIT L 100EUDRAGIT S 100PEROXIDASEPOLYELECTROLYTESPURIFICATIONhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2The separation of radish peroxidase from a fresh Raphanus sativus L extract was carried out using precipitation with two commercially available negatively charged synthetic polyelectrolytes: Eudragit® L 100 and Eudragit® S 100. The enzyme was precipitated by polyelectrolyte addition at pH 4.00. The non-soluble complex formed was separated by simple centrifugation and re-dissolved by a pH change. The recovery of radish peroxidase biological activity was 50% of the initial activity in the homogenate for EuL and 45% for EuS, with 1.5-fold increase in its specific activity. The total Eudragit® concentration to precipitate the enzyme was very low: about 2 × 10 -3% w/v. The volume of the final product decreased to 10% of the feedstock, concentrating the sample up to 10 times.Fil: Woitovich Valetti, Nadia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; ArgentinaFil: Picó, Guillermo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; ArgentinaElsevier Science2013-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/94353Woitovich Valetti, Nadia; Picó, Guillermo Alfredo; A friendly method for Raphanus sativus L (wild radish) peroxidase purification by polyelectrolyte precipitation; Elsevier Science; Separation and Purification Technology; 119; 11-2013; 1-61383-5866CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.seppur.2013.08.042info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1383586613005273info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:18Zoai:ri.conicet.gov.ar:11336/94353instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:18.604CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A friendly method for Raphanus sativus L (wild radish) peroxidase purification by polyelectrolyte precipitation
title A friendly method for Raphanus sativus L (wild radish) peroxidase purification by polyelectrolyte precipitation
spellingShingle A friendly method for Raphanus sativus L (wild radish) peroxidase purification by polyelectrolyte precipitation
Woitovich Valetti, Nadia
EUDRAGIT L 100
EUDRAGIT S 100
PEROXIDASE
POLYELECTROLYTES
PURIFICATION
title_short A friendly method for Raphanus sativus L (wild radish) peroxidase purification by polyelectrolyte precipitation
title_full A friendly method for Raphanus sativus L (wild radish) peroxidase purification by polyelectrolyte precipitation
title_fullStr A friendly method for Raphanus sativus L (wild radish) peroxidase purification by polyelectrolyte precipitation
title_full_unstemmed A friendly method for Raphanus sativus L (wild radish) peroxidase purification by polyelectrolyte precipitation
title_sort A friendly method for Raphanus sativus L (wild radish) peroxidase purification by polyelectrolyte precipitation
dc.creator.none.fl_str_mv Woitovich Valetti, Nadia
Picó, Guillermo Alfredo
author Woitovich Valetti, Nadia
author_facet Woitovich Valetti, Nadia
Picó, Guillermo Alfredo
author_role author
author2 Picó, Guillermo Alfredo
author2_role author
dc.subject.none.fl_str_mv EUDRAGIT L 100
EUDRAGIT S 100
PEROXIDASE
POLYELECTROLYTES
PURIFICATION
topic EUDRAGIT L 100
EUDRAGIT S 100
PEROXIDASE
POLYELECTROLYTES
PURIFICATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The separation of radish peroxidase from a fresh Raphanus sativus L extract was carried out using precipitation with two commercially available negatively charged synthetic polyelectrolytes: Eudragit® L 100 and Eudragit® S 100. The enzyme was precipitated by polyelectrolyte addition at pH 4.00. The non-soluble complex formed was separated by simple centrifugation and re-dissolved by a pH change. The recovery of radish peroxidase biological activity was 50% of the initial activity in the homogenate for EuL and 45% for EuS, with 1.5-fold increase in its specific activity. The total Eudragit® concentration to precipitate the enzyme was very low: about 2 × 10 -3% w/v. The volume of the final product decreased to 10% of the feedstock, concentrating the sample up to 10 times.
Fil: Woitovich Valetti, Nadia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; Argentina
Fil: Picó, Guillermo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; Argentina
description The separation of radish peroxidase from a fresh Raphanus sativus L extract was carried out using precipitation with two commercially available negatively charged synthetic polyelectrolytes: Eudragit® L 100 and Eudragit® S 100. The enzyme was precipitated by polyelectrolyte addition at pH 4.00. The non-soluble complex formed was separated by simple centrifugation and re-dissolved by a pH change. The recovery of radish peroxidase biological activity was 50% of the initial activity in the homogenate for EuL and 45% for EuS, with 1.5-fold increase in its specific activity. The total Eudragit® concentration to precipitate the enzyme was very low: about 2 × 10 -3% w/v. The volume of the final product decreased to 10% of the feedstock, concentrating the sample up to 10 times.
publishDate 2013
dc.date.none.fl_str_mv 2013-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/94353
Woitovich Valetti, Nadia; Picó, Guillermo Alfredo; A friendly method for Raphanus sativus L (wild radish) peroxidase purification by polyelectrolyte precipitation; Elsevier Science; Separation and Purification Technology; 119; 11-2013; 1-6
1383-5866
CONICET Digital
CONICET
url http://hdl.handle.net/11336/94353
identifier_str_mv Woitovich Valetti, Nadia; Picó, Guillermo Alfredo; A friendly method for Raphanus sativus L (wild radish) peroxidase purification by polyelectrolyte precipitation; Elsevier Science; Separation and Purification Technology; 119; 11-2013; 1-6
1383-5866
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.seppur.2013.08.042
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1383586613005273
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269023049351168
score 13.13397

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