Features of bacterial growth and polysaccharide production of Streptococcus pneumoniae serotype 14

Autores
Massaldi, Hugo Alberto; Bessio, M. Inés; Suárez, Norma; Texeira, Esther; Rossi, Silvina; Ferreira, Fernando
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effect of several cultivation conditions on the kinetics of bacterial growth and polysaccharide production of Streptococcus pneumoniae serotype 14 was studied. The presence in the supernatant of serotype-specific CPS (capsular polysaccharide) during growth was followed by size-exclusion HPLC and, in parallel, confirmed by using a specific latex reagent. The agitation level did not affect the production behaviour, whereas pH maintenance above 6 strongly enhanced both growth and CPS production throughout the cultivation period in flasks. Production of high-molecular-mass polysaccharide was found to be maximal between 5 and 6 h of cultivation, at the end of the exponential phase. By laser light scattering, 90% of this purified CPS product showed a Mw (molecular mass) range from 350 to 1500 kDa, with an average Mw of 921 kDa. Extending the culture to 24 h gave rise to a clear shift of the Mw distribution of the polysaccharide to values lower than 100 kDa. These findings may have strong implications for the large-scale manufacture of the polysaccharide and the associated conjugate vaccine.
Fil: Massaldi, Hugo Alberto. Universidad de la Republica Instituto de Higiene; Uruguay. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bessio, M. Inés. Universidad de la Republica Instituto de Higiene; Uruguay
Fil: Suárez, Norma. Universidad de la Republica Instituto de Higiene; Uruguay
Fil: Texeira, Esther. Universidad de la Republica Instituto de Higiene; Uruguay
Fil: Rossi, Silvina. Universidad de la Republica Instituto de Higiene; Uruguay
Fil: Ferreira, Fernando. Universidad de la Republica Instituto de Higiene; Uruguay
Materia
Capsular Polysaccharide
Capsule
Fermentation
Pneumoccocus
Streptococcus Pneumoniae
Vaccine
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/53838
Acceder
id CONICETDig_8660832c8975e58d910c88754a18695c
oai_identifier_str oai:ri.conicet.gov.ar:11336/53838
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Features of bacterial growth and polysaccharide production of Streptococcus pneumoniae serotype 14Massaldi, Hugo AlbertoBessio, M. InésSuárez, NormaTexeira, EstherRossi, SilvinaFerreira, FernandoCapsular PolysaccharideCapsuleFermentationPneumoccocusStreptococcus PneumoniaeVaccinehttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2The effect of several cultivation conditions on the kinetics of bacterial growth and polysaccharide production of Streptococcus pneumoniae serotype 14 was studied. The presence in the supernatant of serotype-specific CPS (capsular polysaccharide) during growth was followed by size-exclusion HPLC and, in parallel, confirmed by using a specific latex reagent. The agitation level did not affect the production behaviour, whereas pH maintenance above 6 strongly enhanced both growth and CPS production throughout the cultivation period in flasks. Production of high-molecular-mass polysaccharide was found to be maximal between 5 and 6 h of cultivation, at the end of the exponential phase. By laser light scattering, 90% of this purified CPS product showed a Mw (molecular mass) range from 350 to 1500 kDa, with an average Mw of 921 kDa. Extending the culture to 24 h gave rise to a clear shift of the Mw distribution of the polysaccharide to values lower than 100 kDa. These findings may have strong implications for the large-scale manufacture of the polysaccharide and the associated conjugate vaccine.Fil: Massaldi, Hugo Alberto. Universidad de la Republica Instituto de Higiene; Uruguay. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bessio, M. Inés. Universidad de la Republica Instituto de Higiene; UruguayFil: Suárez, Norma. Universidad de la Republica Instituto de Higiene; UruguayFil: Texeira, Esther. Universidad de la Republica Instituto de Higiene; UruguayFil: Rossi, Silvina. Universidad de la Republica Instituto de Higiene; UruguayFil: Ferreira, Fernando. Universidad de la Republica Instituto de Higiene; UruguayWiley2010-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53838Massaldi, Hugo Alberto; Bessio, M. Inés; Suárez, Norma; Texeira, Esther; Rossi, Silvina; et al.; Features of bacterial growth and polysaccharide production of Streptococcus pneumoniae serotype 14; Wiley; Biotechnology and Applied Biochemistry; 55; 1; 1-2010; 37-430885-4513CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1042/BA20090218info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:44:40Zoai:ri.conicet.gov.ar:11336/53838instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:44:40.76CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Features of bacterial growth and polysaccharide production of Streptococcus pneumoniae serotype 14
title Features of bacterial growth and polysaccharide production of Streptococcus pneumoniae serotype 14
spellingShingle Features of bacterial growth and polysaccharide production of Streptococcus pneumoniae serotype 14
Massaldi, Hugo Alberto
Capsular Polysaccharide
Capsule
Fermentation
Pneumoccocus
Streptococcus Pneumoniae
Vaccine
title_short Features of bacterial growth and polysaccharide production of Streptococcus pneumoniae serotype 14
title_full Features of bacterial growth and polysaccharide production of Streptococcus pneumoniae serotype 14
title_fullStr Features of bacterial growth and polysaccharide production of Streptococcus pneumoniae serotype 14
title_full_unstemmed Features of bacterial growth and polysaccharide production of Streptococcus pneumoniae serotype 14
title_sort Features of bacterial growth and polysaccharide production of Streptococcus pneumoniae serotype 14
dc.creator.none.fl_str_mv Massaldi, Hugo Alberto
Bessio, M. Inés
Suárez, Norma
Texeira, Esther
Rossi, Silvina
Ferreira, Fernando
author Massaldi, Hugo Alberto
author_facet Massaldi, Hugo Alberto
Bessio, M. Inés
Suárez, Norma
Texeira, Esther
Rossi, Silvina
Ferreira, Fernando
author_role author
author2 Bessio, M. Inés
Suárez, Norma
Texeira, Esther
Rossi, Silvina
Ferreira, Fernando
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Capsular Polysaccharide
Capsule
Fermentation
Pneumoccocus
Streptococcus Pneumoniae
Vaccine
topic Capsular Polysaccharide
Capsule
Fermentation
Pneumoccocus
Streptococcus Pneumoniae
Vaccine
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The effect of several cultivation conditions on the kinetics of bacterial growth and polysaccharide production of Streptococcus pneumoniae serotype 14 was studied. The presence in the supernatant of serotype-specific CPS (capsular polysaccharide) during growth was followed by size-exclusion HPLC and, in parallel, confirmed by using a specific latex reagent. The agitation level did not affect the production behaviour, whereas pH maintenance above 6 strongly enhanced both growth and CPS production throughout the cultivation period in flasks. Production of high-molecular-mass polysaccharide was found to be maximal between 5 and 6 h of cultivation, at the end of the exponential phase. By laser light scattering, 90% of this purified CPS product showed a Mw (molecular mass) range from 350 to 1500 kDa, with an average Mw of 921 kDa. Extending the culture to 24 h gave rise to a clear shift of the Mw distribution of the polysaccharide to values lower than 100 kDa. These findings may have strong implications for the large-scale manufacture of the polysaccharide and the associated conjugate vaccine.
Fil: Massaldi, Hugo Alberto. Universidad de la Republica Instituto de Higiene; Uruguay. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bessio, M. Inés. Universidad de la Republica Instituto de Higiene; Uruguay
Fil: Suárez, Norma. Universidad de la Republica Instituto de Higiene; Uruguay
Fil: Texeira, Esther. Universidad de la Republica Instituto de Higiene; Uruguay
Fil: Rossi, Silvina. Universidad de la Republica Instituto de Higiene; Uruguay
Fil: Ferreira, Fernando. Universidad de la Republica Instituto de Higiene; Uruguay
description The effect of several cultivation conditions on the kinetics of bacterial growth and polysaccharide production of Streptococcus pneumoniae serotype 14 was studied. The presence in the supernatant of serotype-specific CPS (capsular polysaccharide) during growth was followed by size-exclusion HPLC and, in parallel, confirmed by using a specific latex reagent. The agitation level did not affect the production behaviour, whereas pH maintenance above 6 strongly enhanced both growth and CPS production throughout the cultivation period in flasks. Production of high-molecular-mass polysaccharide was found to be maximal between 5 and 6 h of cultivation, at the end of the exponential phase. By laser light scattering, 90% of this purified CPS product showed a Mw (molecular mass) range from 350 to 1500 kDa, with an average Mw of 921 kDa. Extending the culture to 24 h gave rise to a clear shift of the Mw distribution of the polysaccharide to values lower than 100 kDa. These findings may have strong implications for the large-scale manufacture of the polysaccharide and the associated conjugate vaccine.
publishDate 2010
dc.date.none.fl_str_mv 2010-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/53838
Massaldi, Hugo Alberto; Bessio, M. Inés; Suárez, Norma; Texeira, Esther; Rossi, Silvina; et al.; Features of bacterial growth and polysaccharide production of Streptococcus pneumoniae serotype 14; Wiley; Biotechnology and Applied Biochemistry; 55; 1; 1-2010; 37-43
0885-4513
CONICET Digital
CONICET
url http://hdl.handle.net/11336/53838
identifier_str_mv Massaldi, Hugo Alberto; Bessio, M. Inés; Suárez, Norma; Texeira, Esther; Rossi, Silvina; et al.; Features of bacterial growth and polysaccharide production of Streptococcus pneumoniae serotype 14; Wiley; Biotechnology and Applied Biochemistry; 55; 1; 1-2010; 37-43
0885-4513
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1042/BA20090218
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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