Sensitivity of capsular-producing Streptococcus thermophilus strains to bacteriophage adsorption
- Autores
- Rodriguez, Maria Cecilia; Van der Meulen, Roel; Vaningelgem, Frederik; Font, Graciela Maria; Raya, Raul Ricardo; De Vuyst, Luc; Mozzi, Fernanda Beatriz
- Año de publicación
- 2008
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Aims: To determine whether the presence and type of exopolysaccharides (EPS), slime-EPS or capsular, and the structural characteristics of the polymers produced by Streptococcus thermophilus strains could interfere with or be involved in phage adsorption. Methods and Results: Phage-host interactions between eight EPS-producing Strep. thermophilus strains (CRL419, 638, 804, 810, 815, 817, 821, 1190) and five streptococcus specific phages (φYsca, φ3, φ5, φ6, φ8) isolated from Argentinean faulty fermentation failed yoghurts were evaluated. No relationship was found between the EPS chemical composition and the phage sensitivity/resistance phenotype. In general, the capsular-producing strains were more sensitive to phage attacks than the noncapsular-producing strains. Streptococcus thermophilus CRL1190 (capsular-producing) was the only strain sensitive to all bacteriophages and showed the highest efficiency of plating. Phage adsorption to a capsular-negative, EPS low-producing mutant of strain CRL1190 was reduced, especially for φYcsa and φ8. Conclusions: The presence of capsular polysaccharide surrounding the cells of Strep. thermophilus strains could play a role in the adsorption of specific phages to the cells. Significance and Impact of the Study: Capsular-producing Strep. thermophilus strains should be evaluated for their bacteriophage sensitivity if they are included in starter cultures for the fermented food industry.
Fil: Rodriguez, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Van der Meulen, Roel. Vrije Universiteit Brussel. Department of Applied Biological Sciences and Engineering. Research Group of Industrial Microbiology and Food Biotechnolog; Bélgica
Fil: Vaningelgem, Frederik. Vrije Universiteit Brussel. Department of Applied Biological Sciences and Engineering. Research Group of Industrial Microbiology and Food Biotechnolog; Bélgica
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Raya, Raul Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: De Vuyst, Luc. Vrije Universiteit Brussel. Department of Applied Biological Sciences and Engineering. Research Group of Industrial Microbiology and Food Biotechnolog; Bélgica
Fil: Mozzi, Fernanda Beatriz. Vrije Universiteit Brussel. Department of Applied Biological Sciences and Engineering. Research Group of Industrial Microbiology and Food Biotechnolog; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina - Materia
-
Bacteriophages
Capsular Polysaccharides
Exopolysaccharides
Lactic Acid Bacteria - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/55299
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Sensitivity of capsular-producing Streptococcus thermophilus strains to bacteriophage adsorptionRodriguez, Maria CeciliaVan der Meulen, RoelVaningelgem, FrederikFont, Graciela MariaRaya, Raul RicardoDe Vuyst, LucMozzi, Fernanda BeatrizBacteriophagesCapsular PolysaccharidesExopolysaccharidesLactic Acid Bacteriahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Aims: To determine whether the presence and type of exopolysaccharides (EPS), slime-EPS or capsular, and the structural characteristics of the polymers produced by Streptococcus thermophilus strains could interfere with or be involved in phage adsorption. Methods and Results: Phage-host interactions between eight EPS-producing Strep. thermophilus strains (CRL419, 638, 804, 810, 815, 817, 821, 1190) and five streptococcus specific phages (φYsca, φ3, φ5, φ6, φ8) isolated from Argentinean faulty fermentation failed yoghurts were evaluated. No relationship was found between the EPS chemical composition and the phage sensitivity/resistance phenotype. In general, the capsular-producing strains were more sensitive to phage attacks than the noncapsular-producing strains. Streptococcus thermophilus CRL1190 (capsular-producing) was the only strain sensitive to all bacteriophages and showed the highest efficiency of plating. Phage adsorption to a capsular-negative, EPS low-producing mutant of strain CRL1190 was reduced, especially for φYcsa and φ8. Conclusions: The presence of capsular polysaccharide surrounding the cells of Strep. thermophilus strains could play a role in the adsorption of specific phages to the cells. Significance and Impact of the Study: Capsular-producing Strep. thermophilus strains should be evaluated for their bacteriophage sensitivity if they are included in starter cultures for the fermented food industry.Fil: Rodriguez, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Van der Meulen, Roel. Vrije Universiteit Brussel. Department of Applied Biological Sciences and Engineering. Research Group of Industrial Microbiology and Food Biotechnolog; BélgicaFil: Vaningelgem, Frederik. Vrije Universiteit Brussel. Department of Applied Biological Sciences and Engineering. Research Group of Industrial Microbiology and Food Biotechnolog; BélgicaFil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Raya, Raul Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: De Vuyst, Luc. Vrije Universiteit Brussel. Department of Applied Biological Sciences and Engineering. Research Group of Industrial Microbiology and Food Biotechnolog; BélgicaFil: Mozzi, Fernanda Beatriz. Vrije Universiteit Brussel. Department of Applied Biological Sciences and Engineering. Research Group of Industrial Microbiology and Food Biotechnolog; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaWiley Blackwell Publishing, Inc2008-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/55299Rodriguez, Maria Cecilia; Van der Meulen, Roel; Vaningelgem, Frederik; Font, Graciela Maria; Raya, Raul Ricardo; et al.; Sensitivity of capsular-producing Streptococcus thermophilus strains to bacteriophage adsorption; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 46; 4; 4-2008; 462-4680266-82541472-765XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1472-765X.2008.02341.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1472-765X.2008.02341.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:32:48Zoai:ri.conicet.gov.ar:11336/55299instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:32:48.584CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Sensitivity of capsular-producing Streptococcus thermophilus strains to bacteriophage adsorption |
title |
Sensitivity of capsular-producing Streptococcus thermophilus strains to bacteriophage adsorption |
spellingShingle |
Sensitivity of capsular-producing Streptococcus thermophilus strains to bacteriophage adsorption Rodriguez, Maria Cecilia Bacteriophages Capsular Polysaccharides Exopolysaccharides Lactic Acid Bacteria |
title_short |
Sensitivity of capsular-producing Streptococcus thermophilus strains to bacteriophage adsorption |
title_full |
Sensitivity of capsular-producing Streptococcus thermophilus strains to bacteriophage adsorption |
title_fullStr |
Sensitivity of capsular-producing Streptococcus thermophilus strains to bacteriophage adsorption |
title_full_unstemmed |
Sensitivity of capsular-producing Streptococcus thermophilus strains to bacteriophage adsorption |
title_sort |
Sensitivity of capsular-producing Streptococcus thermophilus strains to bacteriophage adsorption |
dc.creator.none.fl_str_mv |
Rodriguez, Maria Cecilia Van der Meulen, Roel Vaningelgem, Frederik Font, Graciela Maria Raya, Raul Ricardo De Vuyst, Luc Mozzi, Fernanda Beatriz |
author |
Rodriguez, Maria Cecilia |
author_facet |
Rodriguez, Maria Cecilia Van der Meulen, Roel Vaningelgem, Frederik Font, Graciela Maria Raya, Raul Ricardo De Vuyst, Luc Mozzi, Fernanda Beatriz |
author_role |
author |
author2 |
Van der Meulen, Roel Vaningelgem, Frederik Font, Graciela Maria Raya, Raul Ricardo De Vuyst, Luc Mozzi, Fernanda Beatriz |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Bacteriophages Capsular Polysaccharides Exopolysaccharides Lactic Acid Bacteria |
topic |
Bacteriophages Capsular Polysaccharides Exopolysaccharides Lactic Acid Bacteria |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Aims: To determine whether the presence and type of exopolysaccharides (EPS), slime-EPS or capsular, and the structural characteristics of the polymers produced by Streptococcus thermophilus strains could interfere with or be involved in phage adsorption. Methods and Results: Phage-host interactions between eight EPS-producing Strep. thermophilus strains (CRL419, 638, 804, 810, 815, 817, 821, 1190) and five streptococcus specific phages (φYsca, φ3, φ5, φ6, φ8) isolated from Argentinean faulty fermentation failed yoghurts were evaluated. No relationship was found between the EPS chemical composition and the phage sensitivity/resistance phenotype. In general, the capsular-producing strains were more sensitive to phage attacks than the noncapsular-producing strains. Streptococcus thermophilus CRL1190 (capsular-producing) was the only strain sensitive to all bacteriophages and showed the highest efficiency of plating. Phage adsorption to a capsular-negative, EPS low-producing mutant of strain CRL1190 was reduced, especially for φYcsa and φ8. Conclusions: The presence of capsular polysaccharide surrounding the cells of Strep. thermophilus strains could play a role in the adsorption of specific phages to the cells. Significance and Impact of the Study: Capsular-producing Strep. thermophilus strains should be evaluated for their bacteriophage sensitivity if they are included in starter cultures for the fermented food industry. Fil: Rodriguez, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Van der Meulen, Roel. Vrije Universiteit Brussel. Department of Applied Biological Sciences and Engineering. Research Group of Industrial Microbiology and Food Biotechnolog; Bélgica Fil: Vaningelgem, Frederik. Vrije Universiteit Brussel. Department of Applied Biological Sciences and Engineering. Research Group of Industrial Microbiology and Food Biotechnolog; Bélgica Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Raya, Raul Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: De Vuyst, Luc. Vrije Universiteit Brussel. Department of Applied Biological Sciences and Engineering. Research Group of Industrial Microbiology and Food Biotechnolog; Bélgica Fil: Mozzi, Fernanda Beatriz. Vrije Universiteit Brussel. Department of Applied Biological Sciences and Engineering. Research Group of Industrial Microbiology and Food Biotechnolog; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina |
description |
Aims: To determine whether the presence and type of exopolysaccharides (EPS), slime-EPS or capsular, and the structural characteristics of the polymers produced by Streptococcus thermophilus strains could interfere with or be involved in phage adsorption. Methods and Results: Phage-host interactions between eight EPS-producing Strep. thermophilus strains (CRL419, 638, 804, 810, 815, 817, 821, 1190) and five streptococcus specific phages (φYsca, φ3, φ5, φ6, φ8) isolated from Argentinean faulty fermentation failed yoghurts were evaluated. No relationship was found between the EPS chemical composition and the phage sensitivity/resistance phenotype. In general, the capsular-producing strains were more sensitive to phage attacks than the noncapsular-producing strains. Streptococcus thermophilus CRL1190 (capsular-producing) was the only strain sensitive to all bacteriophages and showed the highest efficiency of plating. Phage adsorption to a capsular-negative, EPS low-producing mutant of strain CRL1190 was reduced, especially for φYcsa and φ8. Conclusions: The presence of capsular polysaccharide surrounding the cells of Strep. thermophilus strains could play a role in the adsorption of specific phages to the cells. Significance and Impact of the Study: Capsular-producing Strep. thermophilus strains should be evaluated for their bacteriophage sensitivity if they are included in starter cultures for the fermented food industry. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/55299 Rodriguez, Maria Cecilia; Van der Meulen, Roel; Vaningelgem, Frederik; Font, Graciela Maria; Raya, Raul Ricardo; et al.; Sensitivity of capsular-producing Streptococcus thermophilus strains to bacteriophage adsorption; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 46; 4; 4-2008; 462-468 0266-8254 1472-765X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/55299 |
identifier_str_mv |
Rodriguez, Maria Cecilia; Van der Meulen, Roel; Vaningelgem, Frederik; Font, Graciela Maria; Raya, Raul Ricardo; et al.; Sensitivity of capsular-producing Streptococcus thermophilus strains to bacteriophage adsorption; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 46; 4; 4-2008; 462-468 0266-8254 1472-765X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1472-765X.2008.02341.x info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1472-765X.2008.02341.x |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614342586138624 |
score |
13.070432 |