Sensitivity of capsular-producing Streptococcus thermophilus strains to bacteriophage adsorption

Autores
Rodriguez, Maria Cecilia; Van der Meulen, Roel; Vaningelgem, Frederik; Font, Graciela Maria; Raya, Raul Ricardo; De Vuyst, Luc; Mozzi, Fernanda Beatriz
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims: To determine whether the presence and type of exopolysaccharides (EPS), slime-EPS or capsular, and the structural characteristics of the polymers produced by Streptococcus thermophilus strains could interfere with or be involved in phage adsorption. Methods and Results: Phage-host interactions between eight EPS-producing Strep. thermophilus strains (CRL419, 638, 804, 810, 815, 817, 821, 1190) and five streptococcus specific phages (φYsca, φ3, φ5, φ6, φ8) isolated from Argentinean faulty fermentation failed yoghurts were evaluated. No relationship was found between the EPS chemical composition and the phage sensitivity/resistance phenotype. In general, the capsular-producing strains were more sensitive to phage attacks than the noncapsular-producing strains. Streptococcus thermophilus CRL1190 (capsular-producing) was the only strain sensitive to all bacteriophages and showed the highest efficiency of plating. Phage adsorption to a capsular-negative, EPS low-producing mutant of strain CRL1190 was reduced, especially for φYcsa and φ8. Conclusions: The presence of capsular polysaccharide surrounding the cells of Strep. thermophilus strains could play a role in the adsorption of specific phages to the cells. Significance and Impact of the Study: Capsular-producing Strep. thermophilus strains should be evaluated for their bacteriophage sensitivity if they are included in starter cultures for the fermented food industry.
Fil: Rodriguez, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Van der Meulen, Roel. Vrije Universiteit Brussel. Department of Applied Biological Sciences and Engineering. Research Group of Industrial Microbiology and Food Biotechnolog; Bélgica
Fil: Vaningelgem, Frederik. Vrije Universiteit Brussel. Department of Applied Biological Sciences and Engineering. Research Group of Industrial Microbiology and Food Biotechnolog; Bélgica
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Raya, Raul Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: De Vuyst, Luc. Vrije Universiteit Brussel. Department of Applied Biological Sciences and Engineering. Research Group of Industrial Microbiology and Food Biotechnolog; Bélgica
Fil: Mozzi, Fernanda Beatriz. Vrije Universiteit Brussel. Department of Applied Biological Sciences and Engineering. Research Group of Industrial Microbiology and Food Biotechnolog; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Materia
Bacteriophages
Capsular Polysaccharides
Exopolysaccharides
Lactic Acid Bacteria
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/55299
Acceder
id CONICETDig_a9d8e5bd19b5bcc2612966143a2943b1
oai_identifier_str oai:ri.conicet.gov.ar:11336/55299
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sensitivity of capsular-producing Streptococcus thermophilus strains to bacteriophage adsorptionRodriguez, Maria CeciliaVan der Meulen, RoelVaningelgem, FrederikFont, Graciela MariaRaya, Raul RicardoDe Vuyst, LucMozzi, Fernanda BeatrizBacteriophagesCapsular PolysaccharidesExopolysaccharidesLactic Acid Bacteriahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Aims: To determine whether the presence and type of exopolysaccharides (EPS), slime-EPS or capsular, and the structural characteristics of the polymers produced by Streptococcus thermophilus strains could interfere with or be involved in phage adsorption. Methods and Results: Phage-host interactions between eight EPS-producing Strep. thermophilus strains (CRL419, 638, 804, 810, 815, 817, 821, 1190) and five streptococcus specific phages (φYsca, φ3, φ5, φ6, φ8) isolated from Argentinean faulty fermentation failed yoghurts were evaluated. No relationship was found between the EPS chemical composition and the phage sensitivity/resistance phenotype. In general, the capsular-producing strains were more sensitive to phage attacks than the noncapsular-producing strains. Streptococcus thermophilus CRL1190 (capsular-producing) was the only strain sensitive to all bacteriophages and showed the highest efficiency of plating. Phage adsorption to a capsular-negative, EPS low-producing mutant of strain CRL1190 was reduced, especially for φYcsa and φ8. Conclusions: The presence of capsular polysaccharide surrounding the cells of Strep. thermophilus strains could play a role in the adsorption of specific phages to the cells. Significance and Impact of the Study: Capsular-producing Strep. thermophilus strains should be evaluated for their bacteriophage sensitivity if they are included in starter cultures for the fermented food industry.Fil: Rodriguez, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Van der Meulen, Roel. Vrije Universiteit Brussel. Department of Applied Biological Sciences and Engineering. Research Group of Industrial Microbiology and Food Biotechnolog; BélgicaFil: Vaningelgem, Frederik. Vrije Universiteit Brussel. Department of Applied Biological Sciences and Engineering. Research Group of Industrial Microbiology and Food Biotechnolog; BélgicaFil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Raya, Raul Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: De Vuyst, Luc. Vrije Universiteit Brussel. Department of Applied Biological Sciences and Engineering. Research Group of Industrial Microbiology and Food Biotechnolog; BélgicaFil: Mozzi, Fernanda Beatriz. Vrije Universiteit Brussel. Department of Applied Biological Sciences and Engineering. Research Group of Industrial Microbiology and Food Biotechnolog; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaWiley Blackwell Publishing, Inc2008-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/55299Rodriguez, Maria Cecilia; Van der Meulen, Roel; Vaningelgem, Frederik; Font, Graciela Maria; Raya, Raul Ricardo; et al.; Sensitivity of capsular-producing Streptococcus thermophilus strains to bacteriophage adsorption; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 46; 4; 4-2008; 462-4680266-82541472-765XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1472-765X.2008.02341.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1472-765X.2008.02341.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:05:16Zoai:ri.conicet.gov.ar:11336/55299instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:05:17.008CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sensitivity of capsular-producing Streptococcus thermophilus strains to bacteriophage adsorption
title Sensitivity of capsular-producing Streptococcus thermophilus strains to bacteriophage adsorption
spellingShingle Sensitivity of capsular-producing Streptococcus thermophilus strains to bacteriophage adsorption
Rodriguez, Maria Cecilia
Bacteriophages
Capsular Polysaccharides
Exopolysaccharides
Lactic Acid Bacteria
title_short Sensitivity of capsular-producing Streptococcus thermophilus strains to bacteriophage adsorption
title_full Sensitivity of capsular-producing Streptococcus thermophilus strains to bacteriophage adsorption
title_fullStr Sensitivity of capsular-producing Streptococcus thermophilus strains to bacteriophage adsorption
title_full_unstemmed Sensitivity of capsular-producing Streptococcus thermophilus strains to bacteriophage adsorption
title_sort Sensitivity of capsular-producing Streptococcus thermophilus strains to bacteriophage adsorption
dc.creator.none.fl_str_mv Rodriguez, Maria Cecilia
Van der Meulen, Roel
Vaningelgem, Frederik
Font, Graciela Maria
Raya, Raul Ricardo
De Vuyst, Luc
Mozzi, Fernanda Beatriz
author Rodriguez, Maria Cecilia
author_facet Rodriguez, Maria Cecilia
Van der Meulen, Roel
Vaningelgem, Frederik
Font, Graciela Maria
Raya, Raul Ricardo
De Vuyst, Luc
Mozzi, Fernanda Beatriz
author_role author
author2 Van der Meulen, Roel
Vaningelgem, Frederik
Font, Graciela Maria
Raya, Raul Ricardo
De Vuyst, Luc
Mozzi, Fernanda Beatriz
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Bacteriophages
Capsular Polysaccharides
Exopolysaccharides
Lactic Acid Bacteria
topic Bacteriophages
Capsular Polysaccharides
Exopolysaccharides
Lactic Acid Bacteria
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Aims: To determine whether the presence and type of exopolysaccharides (EPS), slime-EPS or capsular, and the structural characteristics of the polymers produced by Streptococcus thermophilus strains could interfere with or be involved in phage adsorption. Methods and Results: Phage-host interactions between eight EPS-producing Strep. thermophilus strains (CRL419, 638, 804, 810, 815, 817, 821, 1190) and five streptococcus specific phages (φYsca, φ3, φ5, φ6, φ8) isolated from Argentinean faulty fermentation failed yoghurts were evaluated. No relationship was found between the EPS chemical composition and the phage sensitivity/resistance phenotype. In general, the capsular-producing strains were more sensitive to phage attacks than the noncapsular-producing strains. Streptococcus thermophilus CRL1190 (capsular-producing) was the only strain sensitive to all bacteriophages and showed the highest efficiency of plating. Phage adsorption to a capsular-negative, EPS low-producing mutant of strain CRL1190 was reduced, especially for φYcsa and φ8. Conclusions: The presence of capsular polysaccharide surrounding the cells of Strep. thermophilus strains could play a role in the adsorption of specific phages to the cells. Significance and Impact of the Study: Capsular-producing Strep. thermophilus strains should be evaluated for their bacteriophage sensitivity if they are included in starter cultures for the fermented food industry.
Fil: Rodriguez, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Van der Meulen, Roel. Vrije Universiteit Brussel. Department of Applied Biological Sciences and Engineering. Research Group of Industrial Microbiology and Food Biotechnolog; Bélgica
Fil: Vaningelgem, Frederik. Vrije Universiteit Brussel. Department of Applied Biological Sciences and Engineering. Research Group of Industrial Microbiology and Food Biotechnolog; Bélgica
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Raya, Raul Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: De Vuyst, Luc. Vrije Universiteit Brussel. Department of Applied Biological Sciences and Engineering. Research Group of Industrial Microbiology and Food Biotechnolog; Bélgica
Fil: Mozzi, Fernanda Beatriz. Vrije Universiteit Brussel. Department of Applied Biological Sciences and Engineering. Research Group of Industrial Microbiology and Food Biotechnolog; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
description Aims: To determine whether the presence and type of exopolysaccharides (EPS), slime-EPS or capsular, and the structural characteristics of the polymers produced by Streptococcus thermophilus strains could interfere with or be involved in phage adsorption. Methods and Results: Phage-host interactions between eight EPS-producing Strep. thermophilus strains (CRL419, 638, 804, 810, 815, 817, 821, 1190) and five streptococcus specific phages (φYsca, φ3, φ5, φ6, φ8) isolated from Argentinean faulty fermentation failed yoghurts were evaluated. No relationship was found between the EPS chemical composition and the phage sensitivity/resistance phenotype. In general, the capsular-producing strains were more sensitive to phage attacks than the noncapsular-producing strains. Streptococcus thermophilus CRL1190 (capsular-producing) was the only strain sensitive to all bacteriophages and showed the highest efficiency of plating. Phage adsorption to a capsular-negative, EPS low-producing mutant of strain CRL1190 was reduced, especially for φYcsa and φ8. Conclusions: The presence of capsular polysaccharide surrounding the cells of Strep. thermophilus strains could play a role in the adsorption of specific phages to the cells. Significance and Impact of the Study: Capsular-producing Strep. thermophilus strains should be evaluated for their bacteriophage sensitivity if they are included in starter cultures for the fermented food industry.
publishDate 2008
dc.date.none.fl_str_mv 2008-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/55299
Rodriguez, Maria Cecilia; Van der Meulen, Roel; Vaningelgem, Frederik; Font, Graciela Maria; Raya, Raul Ricardo; et al.; Sensitivity of capsular-producing Streptococcus thermophilus strains to bacteriophage adsorption; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 46; 4; 4-2008; 462-468
0266-8254
1472-765X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/55299
identifier_str_mv Rodriguez, Maria Cecilia; Van der Meulen, Roel; Vaningelgem, Frederik; Font, Graciela Maria; Raya, Raul Ricardo; et al.; Sensitivity of capsular-producing Streptococcus thermophilus strains to bacteriophage adsorption; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 46; 4; 4-2008; 462-468
0266-8254
1472-765X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1472-765X.2008.02341.x
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1472-765X.2008.02341.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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