A new serotype 14 variant of the pneumococcal Spain9V-3 international clone detected in the central region of Argentina

Autores
Albarracín Orio, Andrea Georgina; Cortes, Paulo; Tregnaghi, Miguel; Piñas, German Eduardo; Echenique, Jose Ricardo; Yudowski, Silvia; Carvajal, Lydia; Culasso, Catalina; Nobile, Carmen Beatriz; Figueroa, Myriam Haydee; Lopardo, Horacio; Hernández, Claudia; Regueira, Mabel
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The penicillin-resistant Spain9V-3 clone of Streptococcus pneumoniae is widespread and presents different serotype variants originating from recombination of the capsular genes. In this work, the genetic relatedness of 29 invasive pneumococci isolated from the central region of Argentina (Cordoba, Buenos Aires, Santa Fe and La Pampa provinces) was assessed by multilocus sequence typing (MLST). All of the penicillin-non-susceptible isolates studied (21/29) belonged to a serotype 14 variant of the Spain 9V-3 clone. This clone was predominant, suggesting that it was responsible for the penicillin resistance spread in this region. Interestingly, this serotype 14 variant (named Cordoba S14V) could be differentiated from the European one by its pbp1a gene, suggesting a different recombinational replacement of the capsular genes. The putative recombination sites were analysed, resulting in the proximal crossover point being clearly localized in the spr0309 gene, with the distal site restricted to the recU gene, confirming a different recombination event. Analysis of the dexB, cpsB, aliA and pbp1a genes from these strains showed a high similarity with the corresponding genes of the Spain14-5 clone, suggesting that the capsular genes were provided by this international clone. Analysis of the genetic polymorphisms of the pbp1a (nt 1473-1922) and spr0309 (nt 1-790) genes is proposed as an epidemiological tool to help recognize the Cordoba S14V of the Spain9V-3 clone. On the other hand, BOX-repeat-based PCR and MLST analyses of serotype 14 strains revealed a divergent epidemiology of the Cordoba S14V, suggesting a non-recent dissemination in the paediatric population. It is suggested that this molecular epidemiology work will be a reference for monitoring the evolution of S14Vs of Spain9V-3, the emergence of new clones and the impact of pneumococcal vaccination programmes in Argentina.
Fil: Albarracín Orio, Andrea Georgina. Universidad Católica de Córdoba. Instituto de Investigaciones en Recursos Naturales y Sustentabilidad José Sanchez Labrador S. J. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Recursos Naturales y Sustentabilidad José Sanchez Labrador S. J.; Argentina
Fil: Cortes, Paulo. Hospital Pediátrico del Niño Jesús; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Tregnaghi, Miguel. Centro de Desarrollo de Proyectos Avanzados en Pediatria; Argentina
Fil: Piñas, German Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Echenique, Jose Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Yudowski, Silvia. Centro de Desarrollo de Proyectos Avanzados en Pediatria; Argentina
Fil: Carvajal, Lydia. Centro de Desarrollo de Proyectos Avanzados en Pediatria; Argentina
Fil: Culasso, Catalina. Centro de Desarrollo de Proyectos Avanzados en Pediatria; Argentina
Fil: Nobile, Carmen Beatriz. Centro de Desarrollo de Proyectos Avanzados en Pediatria; Argentina
Fil: Figueroa, Myriam Haydee. Centro de Desarrollo de Proyectos Avanzados en Pediatria; Argentina
Fil: Lopardo, Horacio. Fundacion Hospital de Pediatria Professor Dr. Juan P. Garrahan; Argentina
Fil: Hernández, Claudia. Fundacion Hospital de Pediatria Professor Dr. Juan P. Garrahan; Argentina
Fil: Regueira, Mabel. Instituto Nacional de Enfermedades Infecciosas; Argentina
Materia
STREPTOCOCCUS PNEUMONIAE
MOLECULAR EPIDEMIOLOGY
PENICILLIN RESISTANCE
CAPSULAR GENES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/132363
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/132363
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A new serotype 14 variant of the pneumococcal Spain9V-3 international clone detected in the central region of ArgentinaAlbarracín Orio, Andrea GeorginaCortes, PauloTregnaghi, MiguelPiñas, German EduardoEchenique, Jose RicardoYudowski, SilviaCarvajal, LydiaCulasso, CatalinaNobile, Carmen BeatrizFigueroa, Myriam HaydeeLopardo, HoracioHernández, ClaudiaRegueira, MabelSTREPTOCOCCUS PNEUMONIAEMOLECULAR EPIDEMIOLOGYPENICILLIN RESISTANCECAPSULAR GENEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The penicillin-resistant Spain9V-3 clone of Streptococcus pneumoniae is widespread and presents different serotype variants originating from recombination of the capsular genes. In this work, the genetic relatedness of 29 invasive pneumococci isolated from the central region of Argentina (Cordoba, Buenos Aires, Santa Fe and La Pampa provinces) was assessed by multilocus sequence typing (MLST). All of the penicillin-non-susceptible isolates studied (21/29) belonged to a serotype 14 variant of the Spain 9V-3 clone. This clone was predominant, suggesting that it was responsible for the penicillin resistance spread in this region. Interestingly, this serotype 14 variant (named Cordoba S14V) could be differentiated from the European one by its pbp1a gene, suggesting a different recombinational replacement of the capsular genes. The putative recombination sites were analysed, resulting in the proximal crossover point being clearly localized in the spr0309 gene, with the distal site restricted to the recU gene, confirming a different recombination event. Analysis of the dexB, cpsB, aliA and pbp1a genes from these strains showed a high similarity with the corresponding genes of the Spain14-5 clone, suggesting that the capsular genes were provided by this international clone. Analysis of the genetic polymorphisms of the pbp1a (nt 1473-1922) and spr0309 (nt 1-790) genes is proposed as an epidemiological tool to help recognize the Cordoba S14V of the Spain9V-3 clone. On the other hand, BOX-repeat-based PCR and MLST analyses of serotype 14 strains revealed a divergent epidemiology of the Cordoba S14V, suggesting a non-recent dissemination in the paediatric population. It is suggested that this molecular epidemiology work will be a reference for monitoring the evolution of S14Vs of Spain9V-3, the emergence of new clones and the impact of pneumococcal vaccination programmes in Argentina.Fil: Albarracín Orio, Andrea Georgina. Universidad Católica de Córdoba. Instituto de Investigaciones en Recursos Naturales y Sustentabilidad José Sanchez Labrador S. J. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Recursos Naturales y Sustentabilidad José Sanchez Labrador S. J.; ArgentinaFil: Cortes, Paulo. Hospital Pediátrico del Niño Jesús; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Tregnaghi, Miguel. Centro de Desarrollo de Proyectos Avanzados en Pediatria; ArgentinaFil: Piñas, German Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Echenique, Jose Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Yudowski, Silvia. Centro de Desarrollo de Proyectos Avanzados en Pediatria; ArgentinaFil: Carvajal, Lydia. Centro de Desarrollo de Proyectos Avanzados en Pediatria; ArgentinaFil: Culasso, Catalina. Centro de Desarrollo de Proyectos Avanzados en Pediatria; ArgentinaFil: Nobile, Carmen Beatriz. Centro de Desarrollo de Proyectos Avanzados en Pediatria; ArgentinaFil: Figueroa, Myriam Haydee. Centro de Desarrollo de Proyectos Avanzados en Pediatria; ArgentinaFil: Lopardo, Horacio. Fundacion Hospital de Pediatria Professor Dr. Juan P. Garrahan; ArgentinaFil: Hernández, Claudia. Fundacion Hospital de Pediatria Professor Dr. Juan P. Garrahan; ArgentinaFil: Regueira, Mabel. Instituto Nacional de Enfermedades Infecciosas; ArgentinaSociety for General Microbiology2008-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/132363Albarracín Orio, Andrea Georgina; Cortes, Paulo; Tregnaghi, Miguel; Piñas, German Eduardo; Echenique, Jose Ricardo; et al.; A new serotype 14 variant of the pneumococcal Spain9V-3 international clone detected in the central region of Argentina; Society for General Microbiology; Journal of Medical Microbiology; 57; 8; 12-2008; 992-9990022-26151473-5644CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1099/jmm.0.2008/000505-0info:eu-repo/semantics/altIdentifier/url/https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.2008/000505-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:26:06Zoai:ri.conicet.gov.ar:11336/132363instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:26:06.403CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A new serotype 14 variant of the pneumococcal Spain9V-3 international clone detected in the central region of Argentina
title A new serotype 14 variant of the pneumococcal Spain9V-3 international clone detected in the central region of Argentina
spellingShingle A new serotype 14 variant of the pneumococcal Spain9V-3 international clone detected in the central region of Argentina
Albarracín Orio, Andrea Georgina
STREPTOCOCCUS PNEUMONIAE
MOLECULAR EPIDEMIOLOGY
PENICILLIN RESISTANCE
CAPSULAR GENES
title_short A new serotype 14 variant of the pneumococcal Spain9V-3 international clone detected in the central region of Argentina
title_full A new serotype 14 variant of the pneumococcal Spain9V-3 international clone detected in the central region of Argentina
title_fullStr A new serotype 14 variant of the pneumococcal Spain9V-3 international clone detected in the central region of Argentina
title_full_unstemmed A new serotype 14 variant of the pneumococcal Spain9V-3 international clone detected in the central region of Argentina
title_sort A new serotype 14 variant of the pneumococcal Spain9V-3 international clone detected in the central region of Argentina
dc.creator.none.fl_str_mv Albarracín Orio, Andrea Georgina
Cortes, Paulo
Tregnaghi, Miguel
Piñas, German Eduardo
Echenique, Jose Ricardo
Yudowski, Silvia
Carvajal, Lydia
Culasso, Catalina
Nobile, Carmen Beatriz
Figueroa, Myriam Haydee
Lopardo, Horacio
Hernández, Claudia
Regueira, Mabel
author Albarracín Orio, Andrea Georgina
author_facet Albarracín Orio, Andrea Georgina
Cortes, Paulo
Tregnaghi, Miguel
Piñas, German Eduardo
Echenique, Jose Ricardo
Yudowski, Silvia
Carvajal, Lydia
Culasso, Catalina
Nobile, Carmen Beatriz
Figueroa, Myriam Haydee
Lopardo, Horacio
Hernández, Claudia
Regueira, Mabel
author_role author
author2 Cortes, Paulo
Tregnaghi, Miguel
Piñas, German Eduardo
Echenique, Jose Ricardo
Yudowski, Silvia
Carvajal, Lydia
Culasso, Catalina
Nobile, Carmen Beatriz
Figueroa, Myriam Haydee
Lopardo, Horacio
Hernández, Claudia
Regueira, Mabel
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv STREPTOCOCCUS PNEUMONIAE
MOLECULAR EPIDEMIOLOGY
PENICILLIN RESISTANCE
CAPSULAR GENES
topic STREPTOCOCCUS PNEUMONIAE
MOLECULAR EPIDEMIOLOGY
PENICILLIN RESISTANCE
CAPSULAR GENES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The penicillin-resistant Spain9V-3 clone of Streptococcus pneumoniae is widespread and presents different serotype variants originating from recombination of the capsular genes. In this work, the genetic relatedness of 29 invasive pneumococci isolated from the central region of Argentina (Cordoba, Buenos Aires, Santa Fe and La Pampa provinces) was assessed by multilocus sequence typing (MLST). All of the penicillin-non-susceptible isolates studied (21/29) belonged to a serotype 14 variant of the Spain 9V-3 clone. This clone was predominant, suggesting that it was responsible for the penicillin resistance spread in this region. Interestingly, this serotype 14 variant (named Cordoba S14V) could be differentiated from the European one by its pbp1a gene, suggesting a different recombinational replacement of the capsular genes. The putative recombination sites were analysed, resulting in the proximal crossover point being clearly localized in the spr0309 gene, with the distal site restricted to the recU gene, confirming a different recombination event. Analysis of the dexB, cpsB, aliA and pbp1a genes from these strains showed a high similarity with the corresponding genes of the Spain14-5 clone, suggesting that the capsular genes were provided by this international clone. Analysis of the genetic polymorphisms of the pbp1a (nt 1473-1922) and spr0309 (nt 1-790) genes is proposed as an epidemiological tool to help recognize the Cordoba S14V of the Spain9V-3 clone. On the other hand, BOX-repeat-based PCR and MLST analyses of serotype 14 strains revealed a divergent epidemiology of the Cordoba S14V, suggesting a non-recent dissemination in the paediatric population. It is suggested that this molecular epidemiology work will be a reference for monitoring the evolution of S14Vs of Spain9V-3, the emergence of new clones and the impact of pneumococcal vaccination programmes in Argentina.
Fil: Albarracín Orio, Andrea Georgina. Universidad Católica de Córdoba. Instituto de Investigaciones en Recursos Naturales y Sustentabilidad José Sanchez Labrador S. J. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Recursos Naturales y Sustentabilidad José Sanchez Labrador S. J.; Argentina
Fil: Cortes, Paulo. Hospital Pediátrico del Niño Jesús; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Tregnaghi, Miguel. Centro de Desarrollo de Proyectos Avanzados en Pediatria; Argentina
Fil: Piñas, German Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Echenique, Jose Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Yudowski, Silvia. Centro de Desarrollo de Proyectos Avanzados en Pediatria; Argentina
Fil: Carvajal, Lydia. Centro de Desarrollo de Proyectos Avanzados en Pediatria; Argentina
Fil: Culasso, Catalina. Centro de Desarrollo de Proyectos Avanzados en Pediatria; Argentina
Fil: Nobile, Carmen Beatriz. Centro de Desarrollo de Proyectos Avanzados en Pediatria; Argentina
Fil: Figueroa, Myriam Haydee. Centro de Desarrollo de Proyectos Avanzados en Pediatria; Argentina
Fil: Lopardo, Horacio. Fundacion Hospital de Pediatria Professor Dr. Juan P. Garrahan; Argentina
Fil: Hernández, Claudia. Fundacion Hospital de Pediatria Professor Dr. Juan P. Garrahan; Argentina
Fil: Regueira, Mabel. Instituto Nacional de Enfermedades Infecciosas; Argentina
description The penicillin-resistant Spain9V-3 clone of Streptococcus pneumoniae is widespread and presents different serotype variants originating from recombination of the capsular genes. In this work, the genetic relatedness of 29 invasive pneumococci isolated from the central region of Argentina (Cordoba, Buenos Aires, Santa Fe and La Pampa provinces) was assessed by multilocus sequence typing (MLST). All of the penicillin-non-susceptible isolates studied (21/29) belonged to a serotype 14 variant of the Spain 9V-3 clone. This clone was predominant, suggesting that it was responsible for the penicillin resistance spread in this region. Interestingly, this serotype 14 variant (named Cordoba S14V) could be differentiated from the European one by its pbp1a gene, suggesting a different recombinational replacement of the capsular genes. The putative recombination sites were analysed, resulting in the proximal crossover point being clearly localized in the spr0309 gene, with the distal site restricted to the recU gene, confirming a different recombination event. Analysis of the dexB, cpsB, aliA and pbp1a genes from these strains showed a high similarity with the corresponding genes of the Spain14-5 clone, suggesting that the capsular genes were provided by this international clone. Analysis of the genetic polymorphisms of the pbp1a (nt 1473-1922) and spr0309 (nt 1-790) genes is proposed as an epidemiological tool to help recognize the Cordoba S14V of the Spain9V-3 clone. On the other hand, BOX-repeat-based PCR and MLST analyses of serotype 14 strains revealed a divergent epidemiology of the Cordoba S14V, suggesting a non-recent dissemination in the paediatric population. It is suggested that this molecular epidemiology work will be a reference for monitoring the evolution of S14Vs of Spain9V-3, the emergence of new clones and the impact of pneumococcal vaccination programmes in Argentina.
publishDate 2008
dc.date.none.fl_str_mv 2008-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/132363
Albarracín Orio, Andrea Georgina; Cortes, Paulo; Tregnaghi, Miguel; Piñas, German Eduardo; Echenique, Jose Ricardo; et al.; A new serotype 14 variant of the pneumococcal Spain9V-3 international clone detected in the central region of Argentina; Society for General Microbiology; Journal of Medical Microbiology; 57; 8; 12-2008; 992-999
0022-2615
1473-5644
CONICET Digital
CONICET
url http://hdl.handle.net/11336/132363
identifier_str_mv Albarracín Orio, Andrea Georgina; Cortes, Paulo; Tregnaghi, Miguel; Piñas, German Eduardo; Echenique, Jose Ricardo; et al.; A new serotype 14 variant of the pneumococcal Spain9V-3 international clone detected in the central region of Argentina; Society for General Microbiology; Journal of Medical Microbiology; 57; 8; 12-2008; 992-999
0022-2615
1473-5644
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1099/jmm.0.2008/000505-0
info:eu-repo/semantics/altIdentifier/url/https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.2008/000505-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society for General Microbiology
publisher.none.fl_str_mv Society for General Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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