A new serotype 14 variant of the pneumococcal Spain9V-3 international clone detected in the central region of Argentina
- Autores
- Albarracín Orio, Andrea Georgina; Cortes, Paulo; Tregnaghi, Miguel; Piñas, German Eduardo; Echenique, Jose Ricardo; Yudowski, Silvia; Carvajal, Lydia; Culasso, Catalina; Nobile, Carmen Beatriz; Figueroa, Myriam Haydee; Lopardo, Horacio; Hernández, Claudia; Regueira, Mabel
- Año de publicación
- 2008
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The penicillin-resistant Spain9V-3 clone of Streptococcus pneumoniae is widespread and presents different serotype variants originating from recombination of the capsular genes. In this work, the genetic relatedness of 29 invasive pneumococci isolated from the central region of Argentina (Cordoba, Buenos Aires, Santa Fe and La Pampa provinces) was assessed by multilocus sequence typing (MLST). All of the penicillin-non-susceptible isolates studied (21/29) belonged to a serotype 14 variant of the Spain 9V-3 clone. This clone was predominant, suggesting that it was responsible for the penicillin resistance spread in this region. Interestingly, this serotype 14 variant (named Cordoba S14V) could be differentiated from the European one by its pbp1a gene, suggesting a different recombinational replacement of the capsular genes. The putative recombination sites were analysed, resulting in the proximal crossover point being clearly localized in the spr0309 gene, with the distal site restricted to the recU gene, confirming a different recombination event. Analysis of the dexB, cpsB, aliA and pbp1a genes from these strains showed a high similarity with the corresponding genes of the Spain14-5 clone, suggesting that the capsular genes were provided by this international clone. Analysis of the genetic polymorphisms of the pbp1a (nt 1473-1922) and spr0309 (nt 1-790) genes is proposed as an epidemiological tool to help recognize the Cordoba S14V of the Spain9V-3 clone. On the other hand, BOX-repeat-based PCR and MLST analyses of serotype 14 strains revealed a divergent epidemiology of the Cordoba S14V, suggesting a non-recent dissemination in the paediatric population. It is suggested that this molecular epidemiology work will be a reference for monitoring the evolution of S14Vs of Spain9V-3, the emergence of new clones and the impact of pneumococcal vaccination programmes in Argentina.
Fil: Albarracín Orio, Andrea Georgina. Universidad Católica de Córdoba. Instituto de Investigaciones en Recursos Naturales y Sustentabilidad José Sanchez Labrador S. J. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Recursos Naturales y Sustentabilidad José Sanchez Labrador S. J.; Argentina
Fil: Cortes, Paulo. Hospital Pediátrico del Niño Jesús; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Tregnaghi, Miguel. Centro de Desarrollo de Proyectos Avanzados en Pediatria; Argentina
Fil: Piñas, German Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Echenique, Jose Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Yudowski, Silvia. Centro de Desarrollo de Proyectos Avanzados en Pediatria; Argentina
Fil: Carvajal, Lydia. Centro de Desarrollo de Proyectos Avanzados en Pediatria; Argentina
Fil: Culasso, Catalina. Centro de Desarrollo de Proyectos Avanzados en Pediatria; Argentina
Fil: Nobile, Carmen Beatriz. Centro de Desarrollo de Proyectos Avanzados en Pediatria; Argentina
Fil: Figueroa, Myriam Haydee. Centro de Desarrollo de Proyectos Avanzados en Pediatria; Argentina
Fil: Lopardo, Horacio. Fundacion Hospital de Pediatria Professor Dr. Juan P. Garrahan; Argentina
Fil: Hernández, Claudia. Fundacion Hospital de Pediatria Professor Dr. Juan P. Garrahan; Argentina
Fil: Regueira, Mabel. Instituto Nacional de Enfermedades Infecciosas; Argentina - Materia
-
STREPTOCOCCUS PNEUMONIAE
MOLECULAR EPIDEMIOLOGY
PENICILLIN RESISTANCE
CAPSULAR GENES - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/132363
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/132363 |
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CONICET Digital (CONICET) |
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A new serotype 14 variant of the pneumococcal Spain9V-3 international clone detected in the central region of ArgentinaAlbarracín Orio, Andrea GeorginaCortes, PauloTregnaghi, MiguelPiñas, German EduardoEchenique, Jose RicardoYudowski, SilviaCarvajal, LydiaCulasso, CatalinaNobile, Carmen BeatrizFigueroa, Myriam HaydeeLopardo, HoracioHernández, ClaudiaRegueira, MabelSTREPTOCOCCUS PNEUMONIAEMOLECULAR EPIDEMIOLOGYPENICILLIN RESISTANCECAPSULAR GENEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The penicillin-resistant Spain9V-3 clone of Streptococcus pneumoniae is widespread and presents different serotype variants originating from recombination of the capsular genes. In this work, the genetic relatedness of 29 invasive pneumococci isolated from the central region of Argentina (Cordoba, Buenos Aires, Santa Fe and La Pampa provinces) was assessed by multilocus sequence typing (MLST). All of the penicillin-non-susceptible isolates studied (21/29) belonged to a serotype 14 variant of the Spain 9V-3 clone. This clone was predominant, suggesting that it was responsible for the penicillin resistance spread in this region. Interestingly, this serotype 14 variant (named Cordoba S14V) could be differentiated from the European one by its pbp1a gene, suggesting a different recombinational replacement of the capsular genes. The putative recombination sites were analysed, resulting in the proximal crossover point being clearly localized in the spr0309 gene, with the distal site restricted to the recU gene, confirming a different recombination event. Analysis of the dexB, cpsB, aliA and pbp1a genes from these strains showed a high similarity with the corresponding genes of the Spain14-5 clone, suggesting that the capsular genes were provided by this international clone. Analysis of the genetic polymorphisms of the pbp1a (nt 1473-1922) and spr0309 (nt 1-790) genes is proposed as an epidemiological tool to help recognize the Cordoba S14V of the Spain9V-3 clone. On the other hand, BOX-repeat-based PCR and MLST analyses of serotype 14 strains revealed a divergent epidemiology of the Cordoba S14V, suggesting a non-recent dissemination in the paediatric population. It is suggested that this molecular epidemiology work will be a reference for monitoring the evolution of S14Vs of Spain9V-3, the emergence of new clones and the impact of pneumococcal vaccination programmes in Argentina.Fil: Albarracín Orio, Andrea Georgina. Universidad Católica de Córdoba. Instituto de Investigaciones en Recursos Naturales y Sustentabilidad José Sanchez Labrador S. J. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Recursos Naturales y Sustentabilidad José Sanchez Labrador S. J.; ArgentinaFil: Cortes, Paulo. Hospital Pediátrico del Niño Jesús; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Tregnaghi, Miguel. Centro de Desarrollo de Proyectos Avanzados en Pediatria; ArgentinaFil: Piñas, German Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Echenique, Jose Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Yudowski, Silvia. Centro de Desarrollo de Proyectos Avanzados en Pediatria; ArgentinaFil: Carvajal, Lydia. Centro de Desarrollo de Proyectos Avanzados en Pediatria; ArgentinaFil: Culasso, Catalina. Centro de Desarrollo de Proyectos Avanzados en Pediatria; ArgentinaFil: Nobile, Carmen Beatriz. Centro de Desarrollo de Proyectos Avanzados en Pediatria; ArgentinaFil: Figueroa, Myriam Haydee. Centro de Desarrollo de Proyectos Avanzados en Pediatria; ArgentinaFil: Lopardo, Horacio. Fundacion Hospital de Pediatria Professor Dr. Juan P. Garrahan; ArgentinaFil: Hernández, Claudia. Fundacion Hospital de Pediatria Professor Dr. Juan P. Garrahan; ArgentinaFil: Regueira, Mabel. Instituto Nacional de Enfermedades Infecciosas; ArgentinaSociety for General Microbiology2008-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/132363Albarracín Orio, Andrea Georgina; Cortes, Paulo; Tregnaghi, Miguel; Piñas, German Eduardo; Echenique, Jose Ricardo; et al.; A new serotype 14 variant of the pneumococcal Spain9V-3 international clone detected in the central region of Argentina; Society for General Microbiology; Journal of Medical Microbiology; 57; 8; 12-2008; 992-9990022-26151473-5644CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1099/jmm.0.2008/000505-0info:eu-repo/semantics/altIdentifier/url/https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.2008/000505-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:56:53Zoai:ri.conicet.gov.ar:11336/132363instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:56:53.366CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
A new serotype 14 variant of the pneumococcal Spain9V-3 international clone detected in the central region of Argentina |
title |
A new serotype 14 variant of the pneumococcal Spain9V-3 international clone detected in the central region of Argentina |
spellingShingle |
A new serotype 14 variant of the pneumococcal Spain9V-3 international clone detected in the central region of Argentina Albarracín Orio, Andrea Georgina STREPTOCOCCUS PNEUMONIAE MOLECULAR EPIDEMIOLOGY PENICILLIN RESISTANCE CAPSULAR GENES |
title_short |
A new serotype 14 variant of the pneumococcal Spain9V-3 international clone detected in the central region of Argentina |
title_full |
A new serotype 14 variant of the pneumococcal Spain9V-3 international clone detected in the central region of Argentina |
title_fullStr |
A new serotype 14 variant of the pneumococcal Spain9V-3 international clone detected in the central region of Argentina |
title_full_unstemmed |
A new serotype 14 variant of the pneumococcal Spain9V-3 international clone detected in the central region of Argentina |
title_sort |
A new serotype 14 variant of the pneumococcal Spain9V-3 international clone detected in the central region of Argentina |
dc.creator.none.fl_str_mv |
Albarracín Orio, Andrea Georgina Cortes, Paulo Tregnaghi, Miguel Piñas, German Eduardo Echenique, Jose Ricardo Yudowski, Silvia Carvajal, Lydia Culasso, Catalina Nobile, Carmen Beatriz Figueroa, Myriam Haydee Lopardo, Horacio Hernández, Claudia Regueira, Mabel |
author |
Albarracín Orio, Andrea Georgina |
author_facet |
Albarracín Orio, Andrea Georgina Cortes, Paulo Tregnaghi, Miguel Piñas, German Eduardo Echenique, Jose Ricardo Yudowski, Silvia Carvajal, Lydia Culasso, Catalina Nobile, Carmen Beatriz Figueroa, Myriam Haydee Lopardo, Horacio Hernández, Claudia Regueira, Mabel |
author_role |
author |
author2 |
Cortes, Paulo Tregnaghi, Miguel Piñas, German Eduardo Echenique, Jose Ricardo Yudowski, Silvia Carvajal, Lydia Culasso, Catalina Nobile, Carmen Beatriz Figueroa, Myriam Haydee Lopardo, Horacio Hernández, Claudia Regueira, Mabel |
author2_role |
author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
STREPTOCOCCUS PNEUMONIAE MOLECULAR EPIDEMIOLOGY PENICILLIN RESISTANCE CAPSULAR GENES |
topic |
STREPTOCOCCUS PNEUMONIAE MOLECULAR EPIDEMIOLOGY PENICILLIN RESISTANCE CAPSULAR GENES |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The penicillin-resistant Spain9V-3 clone of Streptococcus pneumoniae is widespread and presents different serotype variants originating from recombination of the capsular genes. In this work, the genetic relatedness of 29 invasive pneumococci isolated from the central region of Argentina (Cordoba, Buenos Aires, Santa Fe and La Pampa provinces) was assessed by multilocus sequence typing (MLST). All of the penicillin-non-susceptible isolates studied (21/29) belonged to a serotype 14 variant of the Spain 9V-3 clone. This clone was predominant, suggesting that it was responsible for the penicillin resistance spread in this region. Interestingly, this serotype 14 variant (named Cordoba S14V) could be differentiated from the European one by its pbp1a gene, suggesting a different recombinational replacement of the capsular genes. The putative recombination sites were analysed, resulting in the proximal crossover point being clearly localized in the spr0309 gene, with the distal site restricted to the recU gene, confirming a different recombination event. Analysis of the dexB, cpsB, aliA and pbp1a genes from these strains showed a high similarity with the corresponding genes of the Spain14-5 clone, suggesting that the capsular genes were provided by this international clone. Analysis of the genetic polymorphisms of the pbp1a (nt 1473-1922) and spr0309 (nt 1-790) genes is proposed as an epidemiological tool to help recognize the Cordoba S14V of the Spain9V-3 clone. On the other hand, BOX-repeat-based PCR and MLST analyses of serotype 14 strains revealed a divergent epidemiology of the Cordoba S14V, suggesting a non-recent dissemination in the paediatric population. It is suggested that this molecular epidemiology work will be a reference for monitoring the evolution of S14Vs of Spain9V-3, the emergence of new clones and the impact of pneumococcal vaccination programmes in Argentina. Fil: Albarracín Orio, Andrea Georgina. Universidad Católica de Córdoba. Instituto de Investigaciones en Recursos Naturales y Sustentabilidad José Sanchez Labrador S. J. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Recursos Naturales y Sustentabilidad José Sanchez Labrador S. J.; Argentina Fil: Cortes, Paulo. Hospital Pediátrico del Niño Jesús; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Tregnaghi, Miguel. Centro de Desarrollo de Proyectos Avanzados en Pediatria; Argentina Fil: Piñas, German Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Echenique, Jose Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Yudowski, Silvia. Centro de Desarrollo de Proyectos Avanzados en Pediatria; Argentina Fil: Carvajal, Lydia. Centro de Desarrollo de Proyectos Avanzados en Pediatria; Argentina Fil: Culasso, Catalina. Centro de Desarrollo de Proyectos Avanzados en Pediatria; Argentina Fil: Nobile, Carmen Beatriz. Centro de Desarrollo de Proyectos Avanzados en Pediatria; Argentina Fil: Figueroa, Myriam Haydee. Centro de Desarrollo de Proyectos Avanzados en Pediatria; Argentina Fil: Lopardo, Horacio. Fundacion Hospital de Pediatria Professor Dr. Juan P. Garrahan; Argentina Fil: Hernández, Claudia. Fundacion Hospital de Pediatria Professor Dr. Juan P. Garrahan; Argentina Fil: Regueira, Mabel. Instituto Nacional de Enfermedades Infecciosas; Argentina |
description |
The penicillin-resistant Spain9V-3 clone of Streptococcus pneumoniae is widespread and presents different serotype variants originating from recombination of the capsular genes. In this work, the genetic relatedness of 29 invasive pneumococci isolated from the central region of Argentina (Cordoba, Buenos Aires, Santa Fe and La Pampa provinces) was assessed by multilocus sequence typing (MLST). All of the penicillin-non-susceptible isolates studied (21/29) belonged to a serotype 14 variant of the Spain 9V-3 clone. This clone was predominant, suggesting that it was responsible for the penicillin resistance spread in this region. Interestingly, this serotype 14 variant (named Cordoba S14V) could be differentiated from the European one by its pbp1a gene, suggesting a different recombinational replacement of the capsular genes. The putative recombination sites were analysed, resulting in the proximal crossover point being clearly localized in the spr0309 gene, with the distal site restricted to the recU gene, confirming a different recombination event. Analysis of the dexB, cpsB, aliA and pbp1a genes from these strains showed a high similarity with the corresponding genes of the Spain14-5 clone, suggesting that the capsular genes were provided by this international clone. Analysis of the genetic polymorphisms of the pbp1a (nt 1473-1922) and spr0309 (nt 1-790) genes is proposed as an epidemiological tool to help recognize the Cordoba S14V of the Spain9V-3 clone. On the other hand, BOX-repeat-based PCR and MLST analyses of serotype 14 strains revealed a divergent epidemiology of the Cordoba S14V, suggesting a non-recent dissemination in the paediatric population. It is suggested that this molecular epidemiology work will be a reference for monitoring the evolution of S14Vs of Spain9V-3, the emergence of new clones and the impact of pneumococcal vaccination programmes in Argentina. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/132363 Albarracín Orio, Andrea Georgina; Cortes, Paulo; Tregnaghi, Miguel; Piñas, German Eduardo; Echenique, Jose Ricardo; et al.; A new serotype 14 variant of the pneumococcal Spain9V-3 international clone detected in the central region of Argentina; Society for General Microbiology; Journal of Medical Microbiology; 57; 8; 12-2008; 992-999 0022-2615 1473-5644 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/132363 |
identifier_str_mv |
Albarracín Orio, Andrea Georgina; Cortes, Paulo; Tregnaghi, Miguel; Piñas, German Eduardo; Echenique, Jose Ricardo; et al.; A new serotype 14 variant of the pneumococcal Spain9V-3 international clone detected in the central region of Argentina; Society for General Microbiology; Journal of Medical Microbiology; 57; 8; 12-2008; 992-999 0022-2615 1473-5644 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1099/jmm.0.2008/000505-0 info:eu-repo/semantics/altIdentifier/url/https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.2008/000505-0 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Society for General Microbiology |
publisher.none.fl_str_mv |
Society for General Microbiology |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613706011377664 |
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13.070432 |