Analytical tools for elucidating the biological role of melatonin in plants by LC-MS/MS

Autores
Gomez, Federico Jose Vicente; Gatica Hernández, Ismaél Jairo Gabriel; Martinez, Luis Dante; Silva, Maria Fernanda; Cerutti, Estela Soledad
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Melatonin presence in higher plants was recently discovered and the knowledge of its function in vivo is limited. Several studies have recently shown the occurrence of melatonin and related compounds in grapes and wines. The analysis of melatonin in plants and foods represents a highly challenging task due to its wide concentration range, the difficulty in the selection of the extraction solvents because of its amphipathic nature, and the fact that it reacts quickly with other matrix components. Thus, sample processing factors, preparation/cleanup procedures and chromatographic/detection parameters such as HILIC and reverse phase (C8 and C18) chromatographic modes, electrospray ionization and atmospheric pressure chemical ionization in both negative and positive modes were evaluated. Taken together, we have demonstrated that optimal conditions were quite different for each of the matrices under study. A sonication mediated- extraction step was necessary for grape skin (100% v/v methanol) and plant tissues (50% v/v methanol), while wine and must required a solid phase extraction preconcentration step. HILIC chromatography-(+)APCI ionization was better for melatonin standards, while C8-(+)APCI was the best choice for grape skin and C18-(+ESI) was suitable for wine. On the other hand, C8-(+)ESI was the most appropriate for vegetal tissues of Arabidopsis thaliana. Proposed methods were validated, the limits of detection were in the low picograms levels range. The optimized approaches were applied to the determination of melatonin and its isomer in different vegetal/food samples; levels found within the range: 4.9?440 ng g−1.
Fil: Gomez, Federico Jose Vicente. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina;
Fil: Gatica Hernández, Ismaél Jairo Gabriel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina;
Fil: Martinez, Luis Dante. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina;
Fil: Silva, Maria Fernanda. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina;
Fil: Cerutti, Estela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina;
Materia
Chromatography
Mass Spectrometry
Melatonin
Plant Material
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2100

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network_name_str CONICET Digital (CONICET)
spelling Analytical tools for elucidating the biological role of melatonin in plants by LC-MS/MSGomez, Federico Jose VicenteGatica Hernández, Ismaél Jairo GabrielMartinez, Luis DanteSilva, Maria FernandaCerutti, Estela SoledadChromatographyMass SpectrometryMelatoninPlant Materialhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Melatonin presence in higher plants was recently discovered and the knowledge of its function in vivo is limited. Several studies have recently shown the occurrence of melatonin and related compounds in grapes and wines. The analysis of melatonin in plants and foods represents a highly challenging task due to its wide concentration range, the difficulty in the selection of the extraction solvents because of its amphipathic nature, and the fact that it reacts quickly with other matrix components. Thus, sample processing factors, preparation/cleanup procedures and chromatographic/detection parameters such as HILIC and reverse phase (C8 and C18) chromatographic modes, electrospray ionization and atmospheric pressure chemical ionization in both negative and positive modes were evaluated. Taken together, we have demonstrated that optimal conditions were quite different for each of the matrices under study. A sonication mediated- extraction step was necessary for grape skin (100% v/v methanol) and plant tissues (50% v/v methanol), while wine and must required a solid phase extraction preconcentration step. HILIC chromatography-(+)APCI ionization was better for melatonin standards, while C8-(+)APCI was the best choice for grape skin and C18-(+ESI) was suitable for wine. On the other hand, C8-(+)ESI was the most appropriate for vegetal tissues of Arabidopsis thaliana. Proposed methods were validated, the limits of detection were in the low picograms levels range. The optimized approaches were applied to the determination of melatonin and its isomer in different vegetal/food samples; levels found within the range: 4.9?440 ng g−1.Fil: Gomez, Federico Jose Vicente. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina;Fil: Gatica Hernández, Ismaél Jairo Gabriel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina;Fil: Martinez, Luis Dante. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina;Fil: Silva, Maria Fernanda. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina;Fil: Cerutti, Estela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina;Wiley Vch Verlag2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2100Gomez, Federico Jose Vicente; Gatica Hernández, Ismaél Jairo Gabriel; Martinez, Luis Dante; Silva, Maria Fernanda; Cerutti, Estela Soledad; Analytical tools for elucidating the biological role of melatonin in plants by LC-MS/MS; Wiley Vch Verlag; Electrophoresis; 34; 6-2013; 1749-17560173-0835enginfo:eu-repo/semantics/altIdentifier/doi/10.1002/elps.201200569info:eu-repo/semantics/altIdentifier/url/http://www.interscience.wiley.com/info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/elps.201200569/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:14Zoai:ri.conicet.gov.ar:11336/2100instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:15.188CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Analytical tools for elucidating the biological role of melatonin in plants by LC-MS/MS
title Analytical tools for elucidating the biological role of melatonin in plants by LC-MS/MS
spellingShingle Analytical tools for elucidating the biological role of melatonin in plants by LC-MS/MS
Gomez, Federico Jose Vicente
Chromatography
Mass Spectrometry
Melatonin
Plant Material
title_short Analytical tools for elucidating the biological role of melatonin in plants by LC-MS/MS
title_full Analytical tools for elucidating the biological role of melatonin in plants by LC-MS/MS
title_fullStr Analytical tools for elucidating the biological role of melatonin in plants by LC-MS/MS
title_full_unstemmed Analytical tools for elucidating the biological role of melatonin in plants by LC-MS/MS
title_sort Analytical tools for elucidating the biological role of melatonin in plants by LC-MS/MS
dc.creator.none.fl_str_mv Gomez, Federico Jose Vicente
Gatica Hernández, Ismaél Jairo Gabriel
Martinez, Luis Dante
Silva, Maria Fernanda
Cerutti, Estela Soledad
author Gomez, Federico Jose Vicente
author_facet Gomez, Federico Jose Vicente
Gatica Hernández, Ismaél Jairo Gabriel
Martinez, Luis Dante
Silva, Maria Fernanda
Cerutti, Estela Soledad
author_role author
author2 Gatica Hernández, Ismaél Jairo Gabriel
Martinez, Luis Dante
Silva, Maria Fernanda
Cerutti, Estela Soledad
author2_role author
author
author
author
dc.subject.none.fl_str_mv Chromatography
Mass Spectrometry
Melatonin
Plant Material
topic Chromatography
Mass Spectrometry
Melatonin
Plant Material
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Melatonin presence in higher plants was recently discovered and the knowledge of its function in vivo is limited. Several studies have recently shown the occurrence of melatonin and related compounds in grapes and wines. The analysis of melatonin in plants and foods represents a highly challenging task due to its wide concentration range, the difficulty in the selection of the extraction solvents because of its amphipathic nature, and the fact that it reacts quickly with other matrix components. Thus, sample processing factors, preparation/cleanup procedures and chromatographic/detection parameters such as HILIC and reverse phase (C8 and C18) chromatographic modes, electrospray ionization and atmospheric pressure chemical ionization in both negative and positive modes were evaluated. Taken together, we have demonstrated that optimal conditions were quite different for each of the matrices under study. A sonication mediated- extraction step was necessary for grape skin (100% v/v methanol) and plant tissues (50% v/v methanol), while wine and must required a solid phase extraction preconcentration step. HILIC chromatography-(+)APCI ionization was better for melatonin standards, while C8-(+)APCI was the best choice for grape skin and C18-(+ESI) was suitable for wine. On the other hand, C8-(+)ESI was the most appropriate for vegetal tissues of Arabidopsis thaliana. Proposed methods were validated, the limits of detection were in the low picograms levels range. The optimized approaches were applied to the determination of melatonin and its isomer in different vegetal/food samples; levels found within the range: 4.9?440 ng g−1.
Fil: Gomez, Federico Jose Vicente. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina;
Fil: Gatica Hernández, Ismaél Jairo Gabriel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina;
Fil: Martinez, Luis Dante. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina;
Fil: Silva, Maria Fernanda. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina;
Fil: Cerutti, Estela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina;
description Melatonin presence in higher plants was recently discovered and the knowledge of its function in vivo is limited. Several studies have recently shown the occurrence of melatonin and related compounds in grapes and wines. The analysis of melatonin in plants and foods represents a highly challenging task due to its wide concentration range, the difficulty in the selection of the extraction solvents because of its amphipathic nature, and the fact that it reacts quickly with other matrix components. Thus, sample processing factors, preparation/cleanup procedures and chromatographic/detection parameters such as HILIC and reverse phase (C8 and C18) chromatographic modes, electrospray ionization and atmospheric pressure chemical ionization in both negative and positive modes were evaluated. Taken together, we have demonstrated that optimal conditions were quite different for each of the matrices under study. A sonication mediated- extraction step was necessary for grape skin (100% v/v methanol) and plant tissues (50% v/v methanol), while wine and must required a solid phase extraction preconcentration step. HILIC chromatography-(+)APCI ionization was better for melatonin standards, while C8-(+)APCI was the best choice for grape skin and C18-(+ESI) was suitable for wine. On the other hand, C8-(+)ESI was the most appropriate for vegetal tissues of Arabidopsis thaliana. Proposed methods were validated, the limits of detection were in the low picograms levels range. The optimized approaches were applied to the determination of melatonin and its isomer in different vegetal/food samples; levels found within the range: 4.9?440 ng g−1.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2100
Gomez, Federico Jose Vicente; Gatica Hernández, Ismaél Jairo Gabriel; Martinez, Luis Dante; Silva, Maria Fernanda; Cerutti, Estela Soledad; Analytical tools for elucidating the biological role of melatonin in plants by LC-MS/MS; Wiley Vch Verlag; Electrophoresis; 34; 6-2013; 1749-1756
0173-0835
url http://hdl.handle.net/11336/2100
identifier_str_mv Gomez, Federico Jose Vicente; Gatica Hernández, Ismaél Jairo Gabriel; Martinez, Luis Dante; Silva, Maria Fernanda; Cerutti, Estela Soledad; Analytical tools for elucidating the biological role of melatonin in plants by LC-MS/MS; Wiley Vch Verlag; Electrophoresis; 34; 6-2013; 1749-1756
0173-0835
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/elps.201200569
info:eu-repo/semantics/altIdentifier/url/http://www.interscience.wiley.com/
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/elps.201200569/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Vch Verlag
publisher.none.fl_str_mv Wiley Vch Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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