Analytical tools for elucidating the biological role of melatonin in plants by LC-MS/MS
- Autores
- Gomez, Federico Jose Vicente; Gatica Hernández, Ismaél Jairo Gabriel; Martinez, Luis Dante; Silva, Maria Fernanda; Cerutti, Estela Soledad
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Melatonin presence in higher plants was recently discovered and the knowledge of its function in vivo is limited. Several studies have recently shown the occurrence of melatonin and related compounds in grapes and wines. The analysis of melatonin in plants and foods represents a highly challenging task due to its wide concentration range, the difficulty in the selection of the extraction solvents because of its amphipathic nature, and the fact that it reacts quickly with other matrix components. Thus, sample processing factors, preparation/cleanup procedures and chromatographic/detection parameters such as HILIC and reverse phase (C8 and C18) chromatographic modes, electrospray ionization and atmospheric pressure chemical ionization in both negative and positive modes were evaluated. Taken together, we have demonstrated that optimal conditions were quite different for each of the matrices under study. A sonication mediated- extraction step was necessary for grape skin (100% v/v methanol) and plant tissues (50% v/v methanol), while wine and must required a solid phase extraction preconcentration step. HILIC chromatography-(+)APCI ionization was better for melatonin standards, while C8-(+)APCI was the best choice for grape skin and C18-(+ESI) was suitable for wine. On the other hand, C8-(+)ESI was the most appropriate for vegetal tissues of Arabidopsis thaliana. Proposed methods were validated, the limits of detection were in the low picograms levels range. The optimized approaches were applied to the determination of melatonin and its isomer in different vegetal/food samples; levels found within the range: 4.9?440 ng g−1.
Fil: Gomez, Federico Jose Vicente. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina;
Fil: Gatica Hernández, Ismaél Jairo Gabriel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina;
Fil: Martinez, Luis Dante. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina;
Fil: Silva, Maria Fernanda. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina;
Fil: Cerutti, Estela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina; - Materia
-
Chromatography
Mass Spectrometry
Melatonin
Plant Material - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/2100
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Analytical tools for elucidating the biological role of melatonin in plants by LC-MS/MSGomez, Federico Jose VicenteGatica Hernández, Ismaél Jairo GabrielMartinez, Luis DanteSilva, Maria FernandaCerutti, Estela SoledadChromatographyMass SpectrometryMelatoninPlant Materialhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Melatonin presence in higher plants was recently discovered and the knowledge of its function in vivo is limited. Several studies have recently shown the occurrence of melatonin and related compounds in grapes and wines. The analysis of melatonin in plants and foods represents a highly challenging task due to its wide concentration range, the difficulty in the selection of the extraction solvents because of its amphipathic nature, and the fact that it reacts quickly with other matrix components. Thus, sample processing factors, preparation/cleanup procedures and chromatographic/detection parameters such as HILIC and reverse phase (C8 and C18) chromatographic modes, electrospray ionization and atmospheric pressure chemical ionization in both negative and positive modes were evaluated. Taken together, we have demonstrated that optimal conditions were quite different for each of the matrices under study. A sonication mediated- extraction step was necessary for grape skin (100% v/v methanol) and plant tissues (50% v/v methanol), while wine and must required a solid phase extraction preconcentration step. HILIC chromatography-(+)APCI ionization was better for melatonin standards, while C8-(+)APCI was the best choice for grape skin and C18-(+ESI) was suitable for wine. On the other hand, C8-(+)ESI was the most appropriate for vegetal tissues of Arabidopsis thaliana. Proposed methods were validated, the limits of detection were in the low picograms levels range. The optimized approaches were applied to the determination of melatonin and its isomer in different vegetal/food samples; levels found within the range: 4.9?440 ng g−1.Fil: Gomez, Federico Jose Vicente. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina;Fil: Gatica Hernández, Ismaél Jairo Gabriel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina;Fil: Martinez, Luis Dante. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina;Fil: Silva, Maria Fernanda. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina;Fil: Cerutti, Estela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina;Wiley Vch Verlag2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2100Gomez, Federico Jose Vicente; Gatica Hernández, Ismaél Jairo Gabriel; Martinez, Luis Dante; Silva, Maria Fernanda; Cerutti, Estela Soledad; Analytical tools for elucidating the biological role of melatonin in plants by LC-MS/MS; Wiley Vch Verlag; Electrophoresis; 34; 6-2013; 1749-17560173-0835enginfo:eu-repo/semantics/altIdentifier/doi/10.1002/elps.201200569info:eu-repo/semantics/altIdentifier/url/http://www.interscience.wiley.com/info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/elps.201200569/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:14Zoai:ri.conicet.gov.ar:11336/2100instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:15.188CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Analytical tools for elucidating the biological role of melatonin in plants by LC-MS/MS |
title |
Analytical tools for elucidating the biological role of melatonin in plants by LC-MS/MS |
spellingShingle |
Analytical tools for elucidating the biological role of melatonin in plants by LC-MS/MS Gomez, Federico Jose Vicente Chromatography Mass Spectrometry Melatonin Plant Material |
title_short |
Analytical tools for elucidating the biological role of melatonin in plants by LC-MS/MS |
title_full |
Analytical tools for elucidating the biological role of melatonin in plants by LC-MS/MS |
title_fullStr |
Analytical tools for elucidating the biological role of melatonin in plants by LC-MS/MS |
title_full_unstemmed |
Analytical tools for elucidating the biological role of melatonin in plants by LC-MS/MS |
title_sort |
Analytical tools for elucidating the biological role of melatonin in plants by LC-MS/MS |
dc.creator.none.fl_str_mv |
Gomez, Federico Jose Vicente Gatica Hernández, Ismaél Jairo Gabriel Martinez, Luis Dante Silva, Maria Fernanda Cerutti, Estela Soledad |
author |
Gomez, Federico Jose Vicente |
author_facet |
Gomez, Federico Jose Vicente Gatica Hernández, Ismaél Jairo Gabriel Martinez, Luis Dante Silva, Maria Fernanda Cerutti, Estela Soledad |
author_role |
author |
author2 |
Gatica Hernández, Ismaél Jairo Gabriel Martinez, Luis Dante Silva, Maria Fernanda Cerutti, Estela Soledad |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Chromatography Mass Spectrometry Melatonin Plant Material |
topic |
Chromatography Mass Spectrometry Melatonin Plant Material |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Melatonin presence in higher plants was recently discovered and the knowledge of its function in vivo is limited. Several studies have recently shown the occurrence of melatonin and related compounds in grapes and wines. The analysis of melatonin in plants and foods represents a highly challenging task due to its wide concentration range, the difficulty in the selection of the extraction solvents because of its amphipathic nature, and the fact that it reacts quickly with other matrix components. Thus, sample processing factors, preparation/cleanup procedures and chromatographic/detection parameters such as HILIC and reverse phase (C8 and C18) chromatographic modes, electrospray ionization and atmospheric pressure chemical ionization in both negative and positive modes were evaluated. Taken together, we have demonstrated that optimal conditions were quite different for each of the matrices under study. A sonication mediated- extraction step was necessary for grape skin (100% v/v methanol) and plant tissues (50% v/v methanol), while wine and must required a solid phase extraction preconcentration step. HILIC chromatography-(+)APCI ionization was better for melatonin standards, while C8-(+)APCI was the best choice for grape skin and C18-(+ESI) was suitable for wine. On the other hand, C8-(+)ESI was the most appropriate for vegetal tissues of Arabidopsis thaliana. Proposed methods were validated, the limits of detection were in the low picograms levels range. The optimized approaches were applied to the determination of melatonin and its isomer in different vegetal/food samples; levels found within the range: 4.9?440 ng g−1. Fil: Gomez, Federico Jose Vicente. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina; Fil: Gatica Hernández, Ismaél Jairo Gabriel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina; Fil: Martinez, Luis Dante. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina; Fil: Silva, Maria Fernanda. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina; Fil: Cerutti, Estela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina; |
description |
Melatonin presence in higher plants was recently discovered and the knowledge of its function in vivo is limited. Several studies have recently shown the occurrence of melatonin and related compounds in grapes and wines. The analysis of melatonin in plants and foods represents a highly challenging task due to its wide concentration range, the difficulty in the selection of the extraction solvents because of its amphipathic nature, and the fact that it reacts quickly with other matrix components. Thus, sample processing factors, preparation/cleanup procedures and chromatographic/detection parameters such as HILIC and reverse phase (C8 and C18) chromatographic modes, electrospray ionization and atmospheric pressure chemical ionization in both negative and positive modes were evaluated. Taken together, we have demonstrated that optimal conditions were quite different for each of the matrices under study. A sonication mediated- extraction step was necessary for grape skin (100% v/v methanol) and plant tissues (50% v/v methanol), while wine and must required a solid phase extraction preconcentration step. HILIC chromatography-(+)APCI ionization was better for melatonin standards, while C8-(+)APCI was the best choice for grape skin and C18-(+ESI) was suitable for wine. On the other hand, C8-(+)ESI was the most appropriate for vegetal tissues of Arabidopsis thaliana. Proposed methods were validated, the limits of detection were in the low picograms levels range. The optimized approaches were applied to the determination of melatonin and its isomer in different vegetal/food samples; levels found within the range: 4.9?440 ng g−1. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/2100 Gomez, Federico Jose Vicente; Gatica Hernández, Ismaél Jairo Gabriel; Martinez, Luis Dante; Silva, Maria Fernanda; Cerutti, Estela Soledad; Analytical tools for elucidating the biological role of melatonin in plants by LC-MS/MS; Wiley Vch Verlag; Electrophoresis; 34; 6-2013; 1749-1756 0173-0835 |
url |
http://hdl.handle.net/11336/2100 |
identifier_str_mv |
Gomez, Federico Jose Vicente; Gatica Hernández, Ismaél Jairo Gabriel; Martinez, Luis Dante; Silva, Maria Fernanda; Cerutti, Estela Soledad; Analytical tools for elucidating the biological role of melatonin in plants by LC-MS/MS; Wiley Vch Verlag; Electrophoresis; 34; 6-2013; 1749-1756 0173-0835 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1002/elps.201200569 info:eu-repo/semantics/altIdentifier/url/http://www.interscience.wiley.com/ info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/elps.201200569/abstract |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley Vch Verlag |
publisher.none.fl_str_mv |
Wiley Vch Verlag |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613058625798144 |
score |
13.070432 |