Development of a LC-MS/MS methodology for the monitoring of the antichagasic drug benznidazole in human urine samples
- Autores
- Cerutti, Estela Soledad; Marson, Maria Elena; Mastrantonio Garrido, Guido Enrique; Raba, Julio; Martinez, Noelia Anabel
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Monitoring the drug benznidazole in biological fluids is a powerful tool for clinical diagnostic and pharmacological studies in chagasic patients. However, research in this concern needs to be done. The accurate quantitation of this drug in complex matrices represents a highly challenging task complicated by the absence of sensitive analytical methods. It follows that sample processing strategies, preparation/cleanup procedures, and chromatographic/ionization/detection parameters, were evaluated for method optimization. The summation of this work generated a rapid, selective, sensitive methodology based on reversed-phase chromatography–tandem mass spectrometry for the analysis of benznidazole in urine samples. To the best of our knowledge, this is a first report of a LC–MS/MS platform employed for this application. Matrix effect was determined; a 90% of signal suppression was observed. The limits of detection and quantification were 0.75 and 4.85 μg L−1; respectively. The latter allowed the method׳s application to the detection of benznidazole in clinical studies and pharmacological monitoring analysis.
Fil: Cerutti, Estela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Marson, Maria Elena. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina
Fil: Mastrantonio Garrido, Guido Enrique. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Martinez, Noelia Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina - Materia
-
Chagas Disease
Benznidazole
Human Urine
Liquid Chromatography
Tandem Mass Spectrometry - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/5764
Ver los metadatos del registro completo
id |
CONICETDig_66b93b8197baad6df1902313356e7da6 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/5764 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Development of a LC-MS/MS methodology for the monitoring of the antichagasic drug benznidazole in human urine samplesCerutti, Estela SoledadMarson, Maria ElenaMastrantonio Garrido, Guido EnriqueRaba, JulioMartinez, Noelia AnabelChagas DiseaseBenznidazoleHuman UrineLiquid ChromatographyTandem Mass Spectrometryhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Monitoring the drug benznidazole in biological fluids is a powerful tool for clinical diagnostic and pharmacological studies in chagasic patients. However, research in this concern needs to be done. The accurate quantitation of this drug in complex matrices represents a highly challenging task complicated by the absence of sensitive analytical methods. It follows that sample processing strategies, preparation/cleanup procedures, and chromatographic/ionization/detection parameters, were evaluated for method optimization. The summation of this work generated a rapid, selective, sensitive methodology based on reversed-phase chromatography–tandem mass spectrometry for the analysis of benznidazole in urine samples. To the best of our knowledge, this is a first report of a LC–MS/MS platform employed for this application. Matrix effect was determined; a 90% of signal suppression was observed. The limits of detection and quantification were 0.75 and 4.85 μg L−1; respectively. The latter allowed the method׳s application to the detection of benznidazole in clinical studies and pharmacological monitoring analysis.Fil: Cerutti, Estela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaFil: Marson, Maria Elena. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; ArgentinaFil: Mastrantonio Garrido, Guido Enrique. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; ArgentinaFil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaFil: Martinez, Noelia Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaElsevier2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/5764Cerutti, Estela Soledad; Marson, Maria Elena; Mastrantonio Garrido, Guido Enrique; Raba, Julio; Martinez, Noelia Anabel; Development of a LC-MS/MS methodology for the monitoring of the antichagasic drug benznidazole in human urine samples; Elsevier; Talanta; 131; 12-2014; 656-6600039-9140enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0039914014007206info:eu-repo/semantics/altIdentifier/doi/10.1016/j.talanta.2014.08.040info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:02:23Zoai:ri.conicet.gov.ar:11336/5764instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:02:23.966CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Development of a LC-MS/MS methodology for the monitoring of the antichagasic drug benznidazole in human urine samples |
title |
Development of a LC-MS/MS methodology for the monitoring of the antichagasic drug benznidazole in human urine samples |
spellingShingle |
Development of a LC-MS/MS methodology for the monitoring of the antichagasic drug benznidazole in human urine samples Cerutti, Estela Soledad Chagas Disease Benznidazole Human Urine Liquid Chromatography Tandem Mass Spectrometry |
title_short |
Development of a LC-MS/MS methodology for the monitoring of the antichagasic drug benznidazole in human urine samples |
title_full |
Development of a LC-MS/MS methodology for the monitoring of the antichagasic drug benznidazole in human urine samples |
title_fullStr |
Development of a LC-MS/MS methodology for the monitoring of the antichagasic drug benznidazole in human urine samples |
title_full_unstemmed |
Development of a LC-MS/MS methodology for the monitoring of the antichagasic drug benznidazole in human urine samples |
title_sort |
Development of a LC-MS/MS methodology for the monitoring of the antichagasic drug benznidazole in human urine samples |
dc.creator.none.fl_str_mv |
Cerutti, Estela Soledad Marson, Maria Elena Mastrantonio Garrido, Guido Enrique Raba, Julio Martinez, Noelia Anabel |
author |
Cerutti, Estela Soledad |
author_facet |
Cerutti, Estela Soledad Marson, Maria Elena Mastrantonio Garrido, Guido Enrique Raba, Julio Martinez, Noelia Anabel |
author_role |
author |
author2 |
Marson, Maria Elena Mastrantonio Garrido, Guido Enrique Raba, Julio Martinez, Noelia Anabel |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Chagas Disease Benznidazole Human Urine Liquid Chromatography Tandem Mass Spectrometry |
topic |
Chagas Disease Benznidazole Human Urine Liquid Chromatography Tandem Mass Spectrometry |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Monitoring the drug benznidazole in biological fluids is a powerful tool for clinical diagnostic and pharmacological studies in chagasic patients. However, research in this concern needs to be done. The accurate quantitation of this drug in complex matrices represents a highly challenging task complicated by the absence of sensitive analytical methods. It follows that sample processing strategies, preparation/cleanup procedures, and chromatographic/ionization/detection parameters, were evaluated for method optimization. The summation of this work generated a rapid, selective, sensitive methodology based on reversed-phase chromatography–tandem mass spectrometry for the analysis of benznidazole in urine samples. To the best of our knowledge, this is a first report of a LC–MS/MS platform employed for this application. Matrix effect was determined; a 90% of signal suppression was observed. The limits of detection and quantification were 0.75 and 4.85 μg L−1; respectively. The latter allowed the method׳s application to the detection of benznidazole in clinical studies and pharmacological monitoring analysis. Fil: Cerutti, Estela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina Fil: Marson, Maria Elena. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina Fil: Mastrantonio Garrido, Guido Enrique. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina Fil: Martinez, Noelia Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina |
description |
Monitoring the drug benznidazole in biological fluids is a powerful tool for clinical diagnostic and pharmacological studies in chagasic patients. However, research in this concern needs to be done. The accurate quantitation of this drug in complex matrices represents a highly challenging task complicated by the absence of sensitive analytical methods. It follows that sample processing strategies, preparation/cleanup procedures, and chromatographic/ionization/detection parameters, were evaluated for method optimization. The summation of this work generated a rapid, selective, sensitive methodology based on reversed-phase chromatography–tandem mass spectrometry for the analysis of benznidazole in urine samples. To the best of our knowledge, this is a first report of a LC–MS/MS platform employed for this application. Matrix effect was determined; a 90% of signal suppression was observed. The limits of detection and quantification were 0.75 and 4.85 μg L−1; respectively. The latter allowed the method׳s application to the detection of benznidazole in clinical studies and pharmacological monitoring analysis. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/5764 Cerutti, Estela Soledad; Marson, Maria Elena; Mastrantonio Garrido, Guido Enrique; Raba, Julio; Martinez, Noelia Anabel; Development of a LC-MS/MS methodology for the monitoring of the antichagasic drug benznidazole in human urine samples; Elsevier; Talanta; 131; 12-2014; 656-660 0039-9140 |
url |
http://hdl.handle.net/11336/5764 |
identifier_str_mv |
Cerutti, Estela Soledad; Marson, Maria Elena; Mastrantonio Garrido, Guido Enrique; Raba, Julio; Martinez, Noelia Anabel; Development of a LC-MS/MS methodology for the monitoring of the antichagasic drug benznidazole in human urine samples; Elsevier; Talanta; 131; 12-2014; 656-660 0039-9140 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0039914014007206 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.talanta.2014.08.040 info:eu-repo/semantics/altIdentifier/doi/ |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613827974397952 |
score |
13.070432 |