Development of a LC-MS/MS methodology for the monitoring of the antichagasic drug benznidazole in human urine samples

Autores
Cerutti, Estela Soledad; Marson, Maria Elena; Mastrantonio Garrido, Guido Enrique; Raba, Julio; Martinez, Noelia Anabel
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Monitoring the drug benznidazole in biological fluids is a powerful tool for clinical diagnostic and pharmacological studies in chagasic patients. However, research in this concern needs to be done. The accurate quantitation of this drug in complex matrices represents a highly challenging task complicated by the absence of sensitive analytical methods. It follows that sample processing strategies, preparation/cleanup procedures, and chromatographic/ionization/detection parameters, were evaluated for method optimization. The summation of this work generated a rapid, selective, sensitive methodology based on reversed-phase chromatography–tandem mass spectrometry for the analysis of benznidazole in urine samples. To the best of our knowledge, this is a first report of a LC–MS/MS platform employed for this application. Matrix effect was determined; a 90% of signal suppression was observed. The limits of detection and quantification were 0.75 and 4.85 μg L−1; respectively. The latter allowed the method׳s application to the detection of benznidazole in clinical studies and pharmacological monitoring analysis.
Fil: Cerutti, Estela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Marson, Maria Elena. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina
Fil: Mastrantonio Garrido, Guido Enrique. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Martinez, Noelia Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Materia
Chagas Disease
Benznidazole
Human Urine
Liquid Chromatography
Tandem Mass Spectrometry
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/5764
Acceder
id CONICETDig_66b93b8197baad6df1902313356e7da6
oai_identifier_str oai:ri.conicet.gov.ar:11336/5764
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of a LC-MS/MS methodology for the monitoring of the antichagasic drug benznidazole in human urine samplesCerutti, Estela SoledadMarson, Maria ElenaMastrantonio Garrido, Guido EnriqueRaba, JulioMartinez, Noelia AnabelChagas DiseaseBenznidazoleHuman UrineLiquid ChromatographyTandem Mass Spectrometryhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Monitoring the drug benznidazole in biological fluids is a powerful tool for clinical diagnostic and pharmacological studies in chagasic patients. However, research in this concern needs to be done. The accurate quantitation of this drug in complex matrices represents a highly challenging task complicated by the absence of sensitive analytical methods. It follows that sample processing strategies, preparation/cleanup procedures, and chromatographic/ionization/detection parameters, were evaluated for method optimization. The summation of this work generated a rapid, selective, sensitive methodology based on reversed-phase chromatography–tandem mass spectrometry for the analysis of benznidazole in urine samples. To the best of our knowledge, this is a first report of a LC–MS/MS platform employed for this application. Matrix effect was determined; a 90% of signal suppression was observed. The limits of detection and quantification were 0.75 and 4.85 μg L−1; respectively. The latter allowed the method׳s application to the detection of benznidazole in clinical studies and pharmacological monitoring analysis.Fil: Cerutti, Estela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaFil: Marson, Maria Elena. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; ArgentinaFil: Mastrantonio Garrido, Guido Enrique. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; ArgentinaFil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaFil: Martinez, Noelia Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaElsevier2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/5764Cerutti, Estela Soledad; Marson, Maria Elena; Mastrantonio Garrido, Guido Enrique; Raba, Julio; Martinez, Noelia Anabel; Development of a LC-MS/MS methodology for the monitoring of the antichagasic drug benznidazole in human urine samples; Elsevier; Talanta; 131; 12-2014; 656-6600039-9140enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0039914014007206info:eu-repo/semantics/altIdentifier/doi/10.1016/j.talanta.2014.08.040info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:58:30Zoai:ri.conicet.gov.ar:11336/5764instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:58:30.481CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of a LC-MS/MS methodology for the monitoring of the antichagasic drug benznidazole in human urine samples
title Development of a LC-MS/MS methodology for the monitoring of the antichagasic drug benznidazole in human urine samples
spellingShingle Development of a LC-MS/MS methodology for the monitoring of the antichagasic drug benznidazole in human urine samples
Cerutti, Estela Soledad
Chagas Disease
Benznidazole
Human Urine
Liquid Chromatography
Tandem Mass Spectrometry
title_short Development of a LC-MS/MS methodology for the monitoring of the antichagasic drug benznidazole in human urine samples
title_full Development of a LC-MS/MS methodology for the monitoring of the antichagasic drug benznidazole in human urine samples
title_fullStr Development of a LC-MS/MS methodology for the monitoring of the antichagasic drug benznidazole in human urine samples
title_full_unstemmed Development of a LC-MS/MS methodology for the monitoring of the antichagasic drug benznidazole in human urine samples
title_sort Development of a LC-MS/MS methodology for the monitoring of the antichagasic drug benznidazole in human urine samples
dc.creator.none.fl_str_mv Cerutti, Estela Soledad
Marson, Maria Elena
Mastrantonio Garrido, Guido Enrique
Raba, Julio
Martinez, Noelia Anabel
author Cerutti, Estela Soledad
author_facet Cerutti, Estela Soledad
Marson, Maria Elena
Mastrantonio Garrido, Guido Enrique
Raba, Julio
Martinez, Noelia Anabel
author_role author
author2 Marson, Maria Elena
Mastrantonio Garrido, Guido Enrique
Raba, Julio
Martinez, Noelia Anabel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Chagas Disease
Benznidazole
Human Urine
Liquid Chromatography
Tandem Mass Spectrometry
topic Chagas Disease
Benznidazole
Human Urine
Liquid Chromatography
Tandem Mass Spectrometry
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Monitoring the drug benznidazole in biological fluids is a powerful tool for clinical diagnostic and pharmacological studies in chagasic patients. However, research in this concern needs to be done. The accurate quantitation of this drug in complex matrices represents a highly challenging task complicated by the absence of sensitive analytical methods. It follows that sample processing strategies, preparation/cleanup procedures, and chromatographic/ionization/detection parameters, were evaluated for method optimization. The summation of this work generated a rapid, selective, sensitive methodology based on reversed-phase chromatography–tandem mass spectrometry for the analysis of benznidazole in urine samples. To the best of our knowledge, this is a first report of a LC–MS/MS platform employed for this application. Matrix effect was determined; a 90% of signal suppression was observed. The limits of detection and quantification were 0.75 and 4.85 μg L−1; respectively. The latter allowed the method׳s application to the detection of benznidazole in clinical studies and pharmacological monitoring analysis.
Fil: Cerutti, Estela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Marson, Maria Elena. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina
Fil: Mastrantonio Garrido, Guido Enrique. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Martinez, Noelia Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
description Monitoring the drug benznidazole in biological fluids is a powerful tool for clinical diagnostic and pharmacological studies in chagasic patients. However, research in this concern needs to be done. The accurate quantitation of this drug in complex matrices represents a highly challenging task complicated by the absence of sensitive analytical methods. It follows that sample processing strategies, preparation/cleanup procedures, and chromatographic/ionization/detection parameters, were evaluated for method optimization. The summation of this work generated a rapid, selective, sensitive methodology based on reversed-phase chromatography–tandem mass spectrometry for the analysis of benznidazole in urine samples. To the best of our knowledge, this is a first report of a LC–MS/MS platform employed for this application. Matrix effect was determined; a 90% of signal suppression was observed. The limits of detection and quantification were 0.75 and 4.85 μg L−1; respectively. The latter allowed the method׳s application to the detection of benznidazole in clinical studies and pharmacological monitoring analysis.
publishDate 2014
dc.date.none.fl_str_mv 2014-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/5764
Cerutti, Estela Soledad; Marson, Maria Elena; Mastrantonio Garrido, Guido Enrique; Raba, Julio; Martinez, Noelia Anabel; Development of a LC-MS/MS methodology for the monitoring of the antichagasic drug benznidazole in human urine samples; Elsevier; Talanta; 131; 12-2014; 656-660
0039-9140
url http://hdl.handle.net/11336/5764
identifier_str_mv Cerutti, Estela Soledad; Marson, Maria Elena; Mastrantonio Garrido, Guido Enrique; Raba, Julio; Martinez, Noelia Anabel; Development of a LC-MS/MS methodology for the monitoring of the antichagasic drug benznidazole in human urine samples; Elsevier; Talanta; 131; 12-2014; 656-660
0039-9140
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0039914014007206
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.talanta.2014.08.040
info:eu-repo/semantics/altIdentifier/doi/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083125505949696
score 13.22299

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