Integrated analysis of root microbiomes of soybean and wheat from agricultural fields

Autores
Rascovan, Nicolas; Carbonetto, María Belén; Perrig, Diego Sebastián; Diaz, Marisa; Canciani, Wilter; Abalo, Matías; Alloati, Julieta; González Anta, Gustavo; Vazquez, Martin Pablo
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Root associated bacteria are critical for plant growth and health. Understanding the composition and role of root microbiota is crucial toward agricultural practices that are less dependent on chemical fertilization, which has known negative effects on the environment and human health. Here we analyzed the root-associated microbiomes of soybean and wheat under agricultural field conditions. We took samples from 11 different production fields across a large geographic area. We used 16S rRNA pyrosequencing to explore root microbial communities and also obtained 2,007 bacterial isolates from rhizospheres, which were tested for the presence of plant growth promoting (PGP) traits in-vitro. We observed that pH and nitrate content correlated with beta diversity variability of rhizospheric bacterial communities despite the variable field conditions. We described the dominant bacterial groups associated to roots from both crops at a large geographic scale and we found that a high proportion of them (60-70%) showed more than 97% similarity to bacteria from the isolated collection. Moreover, we observed that 55% of the screened isolates presented PGP activities in vitro. These results are a significant step forward in understanding crop-associated microbiomes and suggest that new directions can be taken to promote crop growth and health by modulating root microbiomes.
Fil: Rascovan, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina
Fil: Carbonetto, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina
Fil: Perrig, Diego Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Rizobacter Argentina S. A.; Argentina
Fil: Diaz, Marisa. Rizobacter Argentina S. A.; Argentina
Fil: Canciani, Wilter. Rizobacter Argentina S. A.; Argentina
Fil: Abalo, Matías. Rizobacter Argentina S. A.; Argentina
Fil: Alloati, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina
Fil: González Anta, Gustavo. Rizobacter Argentina S. A.; Argentina
Fil: Vazquez, Martin Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Materia
Genomics
Microbiome
Soybean
Wheat
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79409
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/79409
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Integrated analysis of root microbiomes of soybean and wheat from agricultural fieldsRascovan, NicolasCarbonetto, María BelénPerrig, Diego SebastiánDiaz, MarisaCanciani, WilterAbalo, MatíasAlloati, JulietaGonzález Anta, GustavoVazquez, Martin PabloGenomicsMicrobiomeSoybeanWheathttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Root associated bacteria are critical for plant growth and health. Understanding the composition and role of root microbiota is crucial toward agricultural practices that are less dependent on chemical fertilization, which has known negative effects on the environment and human health. Here we analyzed the root-associated microbiomes of soybean and wheat under agricultural field conditions. We took samples from 11 different production fields across a large geographic area. We used 16S rRNA pyrosequencing to explore root microbial communities and also obtained 2,007 bacterial isolates from rhizospheres, which were tested for the presence of plant growth promoting (PGP) traits in-vitro. We observed that pH and nitrate content correlated with beta diversity variability of rhizospheric bacterial communities despite the variable field conditions. We described the dominant bacterial groups associated to roots from both crops at a large geographic scale and we found that a high proportion of them (60-70%) showed more than 97% similarity to bacteria from the isolated collection. Moreover, we observed that 55% of the screened isolates presented PGP activities in vitro. These results are a significant step forward in understanding crop-associated microbiomes and suggest that new directions can be taken to promote crop growth and health by modulating root microbiomes.Fil: Rascovan, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; ArgentinaFil: Carbonetto, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; ArgentinaFil: Perrig, Diego Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Rizobacter Argentina S. A.; ArgentinaFil: Diaz, Marisa. Rizobacter Argentina S. A.; ArgentinaFil: Canciani, Wilter. Rizobacter Argentina S. A.; ArgentinaFil: Abalo, Matías. Rizobacter Argentina S. A.; ArgentinaFil: Alloati, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; ArgentinaFil: González Anta, Gustavo. Rizobacter Argentina S. A.; ArgentinaFil: Vazquez, Martin Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaNature Publishing Group2016-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79409Rascovan, Nicolas; Carbonetto, María Belén; Perrig, Diego Sebastián; Diaz, Marisa; Canciani, Wilter; et al.; Integrated analysis of root microbiomes of soybean and wheat from agricultural fields; Nature Publishing Group; Scientific Reports; 6; 6-2016; 1-122045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/srep28084info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/srep28084info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:45:40Zoai:ri.conicet.gov.ar:11336/79409instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:45:40.936CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Integrated analysis of root microbiomes of soybean and wheat from agricultural fields
title Integrated analysis of root microbiomes of soybean and wheat from agricultural fields
spellingShingle Integrated analysis of root microbiomes of soybean and wheat from agricultural fields
Rascovan, Nicolas
Genomics
Microbiome
Soybean
Wheat
title_short Integrated analysis of root microbiomes of soybean and wheat from agricultural fields
title_full Integrated analysis of root microbiomes of soybean and wheat from agricultural fields
title_fullStr Integrated analysis of root microbiomes of soybean and wheat from agricultural fields
title_full_unstemmed Integrated analysis of root microbiomes of soybean and wheat from agricultural fields
title_sort Integrated analysis of root microbiomes of soybean and wheat from agricultural fields
dc.creator.none.fl_str_mv Rascovan, Nicolas
Carbonetto, María Belén
Perrig, Diego Sebastián
Diaz, Marisa
Canciani, Wilter
Abalo, Matías
Alloati, Julieta
González Anta, Gustavo
Vazquez, Martin Pablo
author Rascovan, Nicolas
author_facet Rascovan, Nicolas
Carbonetto, María Belén
Perrig, Diego Sebastián
Diaz, Marisa
Canciani, Wilter
Abalo, Matías
Alloati, Julieta
González Anta, Gustavo
Vazquez, Martin Pablo
author_role author
author2 Carbonetto, María Belén
Perrig, Diego Sebastián
Diaz, Marisa
Canciani, Wilter
Abalo, Matías
Alloati, Julieta
González Anta, Gustavo
Vazquez, Martin Pablo
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Genomics
Microbiome
Soybean
Wheat
topic Genomics
Microbiome
Soybean
Wheat
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Root associated bacteria are critical for plant growth and health. Understanding the composition and role of root microbiota is crucial toward agricultural practices that are less dependent on chemical fertilization, which has known negative effects on the environment and human health. Here we analyzed the root-associated microbiomes of soybean and wheat under agricultural field conditions. We took samples from 11 different production fields across a large geographic area. We used 16S rRNA pyrosequencing to explore root microbial communities and also obtained 2,007 bacterial isolates from rhizospheres, which were tested for the presence of plant growth promoting (PGP) traits in-vitro. We observed that pH and nitrate content correlated with beta diversity variability of rhizospheric bacterial communities despite the variable field conditions. We described the dominant bacterial groups associated to roots from both crops at a large geographic scale and we found that a high proportion of them (60-70%) showed more than 97% similarity to bacteria from the isolated collection. Moreover, we observed that 55% of the screened isolates presented PGP activities in vitro. These results are a significant step forward in understanding crop-associated microbiomes and suggest that new directions can be taken to promote crop growth and health by modulating root microbiomes.
Fil: Rascovan, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina
Fil: Carbonetto, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina
Fil: Perrig, Diego Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Rizobacter Argentina S. A.; Argentina
Fil: Diaz, Marisa. Rizobacter Argentina S. A.; Argentina
Fil: Canciani, Wilter. Rizobacter Argentina S. A.; Argentina
Fil: Abalo, Matías. Rizobacter Argentina S. A.; Argentina
Fil: Alloati, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina
Fil: González Anta, Gustavo. Rizobacter Argentina S. A.; Argentina
Fil: Vazquez, Martin Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
description Root associated bacteria are critical for plant growth and health. Understanding the composition and role of root microbiota is crucial toward agricultural practices that are less dependent on chemical fertilization, which has known negative effects on the environment and human health. Here we analyzed the root-associated microbiomes of soybean and wheat under agricultural field conditions. We took samples from 11 different production fields across a large geographic area. We used 16S rRNA pyrosequencing to explore root microbial communities and also obtained 2,007 bacterial isolates from rhizospheres, which were tested for the presence of plant growth promoting (PGP) traits in-vitro. We observed that pH and nitrate content correlated with beta diversity variability of rhizospheric bacterial communities despite the variable field conditions. We described the dominant bacterial groups associated to roots from both crops at a large geographic scale and we found that a high proportion of them (60-70%) showed more than 97% similarity to bacteria from the isolated collection. Moreover, we observed that 55% of the screened isolates presented PGP activities in vitro. These results are a significant step forward in understanding crop-associated microbiomes and suggest that new directions can be taken to promote crop growth and health by modulating root microbiomes.
publishDate 2016
dc.date.none.fl_str_mv 2016-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79409
Rascovan, Nicolas; Carbonetto, María Belén; Perrig, Diego Sebastián; Diaz, Marisa; Canciani, Wilter; et al.; Integrated analysis of root microbiomes of soybean and wheat from agricultural fields; Nature Publishing Group; Scientific Reports; 6; 6-2016; 1-12
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79409
identifier_str_mv Rascovan, Nicolas; Carbonetto, María Belén; Perrig, Diego Sebastián; Diaz, Marisa; Canciani, Wilter; et al.; Integrated analysis of root microbiomes of soybean and wheat from agricultural fields; Nature Publishing Group; Scientific Reports; 6; 6-2016; 1-12
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/srep28084
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/srep28084
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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