Oro-ruminal sampling device and technique for rapid collection of rumen content and improved recovery of solid fractions for microbiome analysis

Autores
Miccoli, Florencia Estefanía; Galarza, Roxana Ivon; Juliano, Nicolás; Ferreyra, S.; Maresca, Sebastian; Lopez Valiente, Sebastian; Guerrero, Leandro Demian; Palladino, Rafael Alejandro; Albornoz, Rodrigo I.
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Rumen fistulation is a widely used procedure that allows for collection of ruminal contents. However, fistulation is an invasive and costly procedure that generally limits the number of animals that can be recruited for experiments, thus encouraging the use of alternative techniques such as the intra-esophageal tube technique. One of the challenges of this technique is the limited ability to collect solid fractions from the rumen content pool which may impact the microbial community structure in the sample, particularly affecting the recovery and characterization of solid adherent-bacteria. We developed an intra-esophageal tube rumen sampling technique and device referred to as ‘Rumen Sampler MG’ with the aim of increasing the recovery of solid fractions from rumen content compared with other oro-ruminal sampling methods. The ‘Rumen Sampler MG’ device consists of a manual pump fitted with a barometer and an intra-esophageal flexible PVC tube with beveled terminal edge allowing for minimal clogging of the sampling tubing and a rapid flux of contents being sampled. Nine lactating Holstein-Friesian dairy cows (554.6 ± 25.2 kg BW; 8.3 ± 3.3 DIM) were recruited to evaluate the proposed method. During the procedure, animals were safely restrained in a chute and administered with a low dosage of a neuroleptic drug to reduce animal stress during sampling. An endoscopic camera was inserted into the reticulo-rumen through the esophagus to identify the sampling location and determine the length of the sampling tube necessary to reach the desired location. Following, the intra-esophageal sampling tube connected to a manual pump was inserted for collection of rumen contents. Samples collected did not present visual evidence of saliva contamination (e.g., high viscosity) and their pH ranged within expected values (6.33 - 7.04) for samples collected from the reticulo-rumen. Each sample contained 35–40% wet solids volume. Individual dry matter intake and milk production of cows continued to increase after sampling as expected for cows in the early postpartum period, suggesting that the sampling procedure did not affect cow performance. Results from microbiome analysis of rumen content samples suggest that the relative abundances of the main bacterial phyla are consistent with those from samples collected from dairy cows via rumen fistula in previous studies. The device and technique proposed allow for adequate samples of ruminal liquid and solid contents to be collected for microbiome analysis without disruption of animal performance.
EEA Cuenca del Salado
Fil: Miccoli, Florencia. Universidad Nacional de Lomas de Zamora. Facultad de Ciencias Agrarias; Argentina
Fil: Miccoli, Florencia. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal; Argentina
Fil: Galarza, Roxana Ivon. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cuenca del Salado. Agencia de Extensión Rural Chascomús; Argentina
Fil: Juliano, N. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal; Argentina
Fil: Juliano, N. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular “Dr. Héctor N. Torres”; Argentina
Fil: Ferreyra, S. Universidad Nacional de Lomas de Zamora. Facultad de Ciencias Agrarias; Argentina
Fil: Maresca, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cuenca del Salado; Argentina
Fil: Lopez Valiente, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cuenca del Salado; Argentina
Fil: Guerrero, Leandro Demian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular “Dr. Héctor N. Torres”; Argentina
Fil: Palladino, Rafael Alejandro. Universidad Nacional de Lomas de Zamora. Facultad de Ciencias Agrarias; Argentina
Fil: Palladino, Rafael Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular “Dr. Héctor N. Torres”; Argentina
Fil: Albornoz, Rodrigo I. Dairy Australia; Australia
Fuente
JDS Communications : 1-5 (Available online 31 May 2024)
Materia
Rumen
Sampling
Cattle
Microbiomes
Muestreo
Ganado Bovino
Microbiomas
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/18093
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spelling Oro-ruminal sampling device and technique for rapid collection of rumen content and improved recovery of solid fractions for microbiome analysisMiccoli, Florencia EstefaníaGalarza, Roxana IvonJuliano, NicolásFerreyra, S.Maresca, SebastianLopez Valiente, SebastianGuerrero, Leandro DemianPalladino, Rafael AlejandroAlbornoz, Rodrigo I.RumenSamplingCattleMicrobiomesMuestreoGanado BovinoMicrobiomasRumen fistulation is a widely used procedure that allows for collection of ruminal contents. However, fistulation is an invasive and costly procedure that generally limits the number of animals that can be recruited for experiments, thus encouraging the use of alternative techniques such as the intra-esophageal tube technique. One of the challenges of this technique is the limited ability to collect solid fractions from the rumen content pool which may impact the microbial community structure in the sample, particularly affecting the recovery and characterization of solid adherent-bacteria. We developed an intra-esophageal tube rumen sampling technique and device referred to as ‘Rumen Sampler MG’ with the aim of increasing the recovery of solid fractions from rumen content compared with other oro-ruminal sampling methods. The ‘Rumen Sampler MG’ device consists of a manual pump fitted with a barometer and an intra-esophageal flexible PVC tube with beveled terminal edge allowing for minimal clogging of the sampling tubing and a rapid flux of contents being sampled. Nine lactating Holstein-Friesian dairy cows (554.6 ± 25.2 kg BW; 8.3 ± 3.3 DIM) were recruited to evaluate the proposed method. During the procedure, animals were safely restrained in a chute and administered with a low dosage of a neuroleptic drug to reduce animal stress during sampling. An endoscopic camera was inserted into the reticulo-rumen through the esophagus to identify the sampling location and determine the length of the sampling tube necessary to reach the desired location. Following, the intra-esophageal sampling tube connected to a manual pump was inserted for collection of rumen contents. Samples collected did not present visual evidence of saliva contamination (e.g., high viscosity) and their pH ranged within expected values (6.33 - 7.04) for samples collected from the reticulo-rumen. Each sample contained 35–40% wet solids volume. Individual dry matter intake and milk production of cows continued to increase after sampling as expected for cows in the early postpartum period, suggesting that the sampling procedure did not affect cow performance. Results from microbiome analysis of rumen content samples suggest that the relative abundances of the main bacterial phyla are consistent with those from samples collected from dairy cows via rumen fistula in previous studies. The device and technique proposed allow for adequate samples of ruminal liquid and solid contents to be collected for microbiome analysis without disruption of animal performance.EEA Cuenca del SaladoFil: Miccoli, Florencia. Universidad Nacional de Lomas de Zamora. Facultad de Ciencias Agrarias; ArgentinaFil: Miccoli, Florencia. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal; ArgentinaFil: Galarza, Roxana Ivon. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cuenca del Salado. Agencia de Extensión Rural Chascomús; ArgentinaFil: Juliano, N. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal; ArgentinaFil: Juliano, N. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular “Dr. Héctor N. Torres”; ArgentinaFil: Ferreyra, S. Universidad Nacional de Lomas de Zamora. Facultad de Ciencias Agrarias; ArgentinaFil: Maresca, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cuenca del Salado; ArgentinaFil: Lopez Valiente, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cuenca del Salado; ArgentinaFil: Guerrero, Leandro Demian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular “Dr. Héctor N. Torres”; ArgentinaFil: Palladino, Rafael Alejandro. Universidad Nacional de Lomas de Zamora. Facultad de Ciencias Agrarias; ArgentinaFil: Palladino, Rafael Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular “Dr. Héctor N. Torres”; ArgentinaFil: Albornoz, Rodrigo I. Dairy Australia; AustraliaElsevier2024-06-10T11:52:00Z2024-06-10T11:52:00Z2024-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/18093https://www.sciencedirect.com/science/article/pii/S26669102240009302666-9102https://doi.org/10.3168/jdsc.2023-0536JDS Communications : 1-5 (Available online 31 May 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-11-27T08:40:03Zoai:localhost:20.500.12123/18093instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-11-27 08:40:03.6INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Oro-ruminal sampling device and technique for rapid collection of rumen content and improved recovery of solid fractions for microbiome analysis
title Oro-ruminal sampling device and technique for rapid collection of rumen content and improved recovery of solid fractions for microbiome analysis
spellingShingle Oro-ruminal sampling device and technique for rapid collection of rumen content and improved recovery of solid fractions for microbiome analysis
Miccoli, Florencia Estefanía
Rumen
Sampling
Cattle
Microbiomes
Muestreo
Ganado Bovino
Microbiomas
title_short Oro-ruminal sampling device and technique for rapid collection of rumen content and improved recovery of solid fractions for microbiome analysis
title_full Oro-ruminal sampling device and technique for rapid collection of rumen content and improved recovery of solid fractions for microbiome analysis
title_fullStr Oro-ruminal sampling device and technique for rapid collection of rumen content and improved recovery of solid fractions for microbiome analysis
title_full_unstemmed Oro-ruminal sampling device and technique for rapid collection of rumen content and improved recovery of solid fractions for microbiome analysis
title_sort Oro-ruminal sampling device and technique for rapid collection of rumen content and improved recovery of solid fractions for microbiome analysis
dc.creator.none.fl_str_mv Miccoli, Florencia Estefanía
Galarza, Roxana Ivon
Juliano, Nicolás
Ferreyra, S.
Maresca, Sebastian
Lopez Valiente, Sebastian
Guerrero, Leandro Demian
Palladino, Rafael Alejandro
Albornoz, Rodrigo I.
author Miccoli, Florencia Estefanía
author_facet Miccoli, Florencia Estefanía
Galarza, Roxana Ivon
Juliano, Nicolás
Ferreyra, S.
Maresca, Sebastian
Lopez Valiente, Sebastian
Guerrero, Leandro Demian
Palladino, Rafael Alejandro
Albornoz, Rodrigo I.
author_role author
author2 Galarza, Roxana Ivon
Juliano, Nicolás
Ferreyra, S.
Maresca, Sebastian
Lopez Valiente, Sebastian
Guerrero, Leandro Demian
Palladino, Rafael Alejandro
Albornoz, Rodrigo I.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Rumen
Sampling
Cattle
Microbiomes
Muestreo
Ganado Bovino
Microbiomas
topic Rumen
Sampling
Cattle
Microbiomes
Muestreo
Ganado Bovino
Microbiomas
dc.description.none.fl_txt_mv Rumen fistulation is a widely used procedure that allows for collection of ruminal contents. However, fistulation is an invasive and costly procedure that generally limits the number of animals that can be recruited for experiments, thus encouraging the use of alternative techniques such as the intra-esophageal tube technique. One of the challenges of this technique is the limited ability to collect solid fractions from the rumen content pool which may impact the microbial community structure in the sample, particularly affecting the recovery and characterization of solid adherent-bacteria. We developed an intra-esophageal tube rumen sampling technique and device referred to as ‘Rumen Sampler MG’ with the aim of increasing the recovery of solid fractions from rumen content compared with other oro-ruminal sampling methods. The ‘Rumen Sampler MG’ device consists of a manual pump fitted with a barometer and an intra-esophageal flexible PVC tube with beveled terminal edge allowing for minimal clogging of the sampling tubing and a rapid flux of contents being sampled. Nine lactating Holstein-Friesian dairy cows (554.6 ± 25.2 kg BW; 8.3 ± 3.3 DIM) were recruited to evaluate the proposed method. During the procedure, animals were safely restrained in a chute and administered with a low dosage of a neuroleptic drug to reduce animal stress during sampling. An endoscopic camera was inserted into the reticulo-rumen through the esophagus to identify the sampling location and determine the length of the sampling tube necessary to reach the desired location. Following, the intra-esophageal sampling tube connected to a manual pump was inserted for collection of rumen contents. Samples collected did not present visual evidence of saliva contamination (e.g., high viscosity) and their pH ranged within expected values (6.33 - 7.04) for samples collected from the reticulo-rumen. Each sample contained 35–40% wet solids volume. Individual dry matter intake and milk production of cows continued to increase after sampling as expected for cows in the early postpartum period, suggesting that the sampling procedure did not affect cow performance. Results from microbiome analysis of rumen content samples suggest that the relative abundances of the main bacterial phyla are consistent with those from samples collected from dairy cows via rumen fistula in previous studies. The device and technique proposed allow for adequate samples of ruminal liquid and solid contents to be collected for microbiome analysis without disruption of animal performance.
EEA Cuenca del Salado
Fil: Miccoli, Florencia. Universidad Nacional de Lomas de Zamora. Facultad de Ciencias Agrarias; Argentina
Fil: Miccoli, Florencia. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal; Argentina
Fil: Galarza, Roxana Ivon. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cuenca del Salado. Agencia de Extensión Rural Chascomús; Argentina
Fil: Juliano, N. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal; Argentina
Fil: Juliano, N. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular “Dr. Héctor N. Torres”; Argentina
Fil: Ferreyra, S. Universidad Nacional de Lomas de Zamora. Facultad de Ciencias Agrarias; Argentina
Fil: Maresca, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cuenca del Salado; Argentina
Fil: Lopez Valiente, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cuenca del Salado; Argentina
Fil: Guerrero, Leandro Demian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular “Dr. Héctor N. Torres”; Argentina
Fil: Palladino, Rafael Alejandro. Universidad Nacional de Lomas de Zamora. Facultad de Ciencias Agrarias; Argentina
Fil: Palladino, Rafael Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular “Dr. Héctor N. Torres”; Argentina
Fil: Albornoz, Rodrigo I. Dairy Australia; Australia
description Rumen fistulation is a widely used procedure that allows for collection of ruminal contents. However, fistulation is an invasive and costly procedure that generally limits the number of animals that can be recruited for experiments, thus encouraging the use of alternative techniques such as the intra-esophageal tube technique. One of the challenges of this technique is the limited ability to collect solid fractions from the rumen content pool which may impact the microbial community structure in the sample, particularly affecting the recovery and characterization of solid adherent-bacteria. We developed an intra-esophageal tube rumen sampling technique and device referred to as ‘Rumen Sampler MG’ with the aim of increasing the recovery of solid fractions from rumen content compared with other oro-ruminal sampling methods. The ‘Rumen Sampler MG’ device consists of a manual pump fitted with a barometer and an intra-esophageal flexible PVC tube with beveled terminal edge allowing for minimal clogging of the sampling tubing and a rapid flux of contents being sampled. Nine lactating Holstein-Friesian dairy cows (554.6 ± 25.2 kg BW; 8.3 ± 3.3 DIM) were recruited to evaluate the proposed method. During the procedure, animals were safely restrained in a chute and administered with a low dosage of a neuroleptic drug to reduce animal stress during sampling. An endoscopic camera was inserted into the reticulo-rumen through the esophagus to identify the sampling location and determine the length of the sampling tube necessary to reach the desired location. Following, the intra-esophageal sampling tube connected to a manual pump was inserted for collection of rumen contents. Samples collected did not present visual evidence of saliva contamination (e.g., high viscosity) and their pH ranged within expected values (6.33 - 7.04) for samples collected from the reticulo-rumen. Each sample contained 35–40% wet solids volume. Individual dry matter intake and milk production of cows continued to increase after sampling as expected for cows in the early postpartum period, suggesting that the sampling procedure did not affect cow performance. Results from microbiome analysis of rumen content samples suggest that the relative abundances of the main bacterial phyla are consistent with those from samples collected from dairy cows via rumen fistula in previous studies. The device and technique proposed allow for adequate samples of ruminal liquid and solid contents to be collected for microbiome analysis without disruption of animal performance.
publishDate 2024
dc.date.none.fl_str_mv 2024-06-10T11:52:00Z
2024-06-10T11:52:00Z
2024-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/18093
https://www.sciencedirect.com/science/article/pii/S2666910224000930
2666-9102
https://doi.org/10.3168/jdsc.2023-0536
url http://hdl.handle.net/20.500.12123/18093
https://www.sciencedirect.com/science/article/pii/S2666910224000930
https://doi.org/10.3168/jdsc.2023-0536
identifier_str_mv 2666-9102
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv JDS Communications : 1-5 (Available online 31 May 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.011256

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