Effect of melatonin on the retinal glutamate/glutamine cycle in the golden hamster retina

Autores
Saenz, Daniel Alberto; Goldin, Andrea Paula; Minces, Luciana; Chianelli, Mónica Silvia; Keller Sarmiento, María Inés; Rosenstein, Ruth Estela
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Glutamate is the main excitatory neurotransmitter in the retina, but it is neurotoxic when present in excessive amounts. The metabolic dependence of glutamatergic neurons upon glia via the glutamate/glutamine cycle to provide the precursor for neurotransmitter glutamate is well established. Since melatonin has been shown to be neuroprotective in several systems, in the present report, its effect on the glutamate/glutamine cycle activity was examined in the golden hamster retina. Melatonin (0.1−10 nM) significantly increased retinal glutamine synthetase activity but it did not affect L-glutamine release. A characterization of the hamster retinal Lglutamine uptake mechanism was performed. This mechanism was partly Na+ -dependent, and it was significantly inhibited by 2-aminobicyclo (2, 2, 1) heptane 2-carboxylic acid (BCH, a selective antagonists for the L-type system) and by α-(methylamino)-isobutyric acid (MeAIB, substrate characteristic for the A -type transporter) suggesting the coexistence of these transport systems in the hamster retina. Melatonin (0.1−10 nM) significantly increased total glutamine uptake as well as the BCH and the MeAIB-insensitive transporters activity. On the other hand, melatonin significantly decreased retinal glutaminase activity. On the basis of these results, it might be presumed that hamster retinal glutamate/glutamine cycle activity is regulated by physiological concentrations of melatonin. Furthermore, these findings suggest that a treatment with melatonin could be considered as a new approach to handling glutamate-mediated neuronal degeneration.
Fil: Saenz, Daniel Alberto. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Goldin, Andrea Paula. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Minces, Luciana. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Chianelli, Mónica Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Keller Sarmiento, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Rosenstein, Ruth Estela. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
glutamine synthetase
L-glutamine influx
glutaminase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/242110
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/242110
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of melatonin on the retinal glutamate/glutamine cycle in the golden hamster retinaSaenz, Daniel AlbertoGoldin, Andrea PaulaMinces, LucianaChianelli, Mónica SilviaKeller Sarmiento, María InésRosenstein, Ruth Estelaglutamine synthetaseL-glutamine influxglutaminasehttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Glutamate is the main excitatory neurotransmitter in the retina, but it is neurotoxic when present in excessive amounts. The metabolic dependence of glutamatergic neurons upon glia via the glutamate/glutamine cycle to provide the precursor for neurotransmitter glutamate is well established. Since melatonin has been shown to be neuroprotective in several systems, in the present report, its effect on the glutamate/glutamine cycle activity was examined in the golden hamster retina. Melatonin (0.1−10 nM) significantly increased retinal glutamine synthetase activity but it did not affect L-glutamine release. A characterization of the hamster retinal Lglutamine uptake mechanism was performed. This mechanism was partly Na+ -dependent, and it was significantly inhibited by 2-aminobicyclo (2, 2, 1) heptane 2-carboxylic acid (BCH, a selective antagonists for the L-type system) and by α-(methylamino)-isobutyric acid (MeAIB, substrate characteristic for the A -type transporter) suggesting the coexistence of these transport systems in the hamster retina. Melatonin (0.1−10 nM) significantly increased total glutamine uptake as well as the BCH and the MeAIB-insensitive transporters activity. On the other hand, melatonin significantly decreased retinal glutaminase activity. On the basis of these results, it might be presumed that hamster retinal glutamate/glutamine cycle activity is regulated by physiological concentrations of melatonin. Furthermore, these findings suggest that a treatment with melatonin could be considered as a new approach to handling glutamate-mediated neuronal degeneration.Fil: Saenz, Daniel Alberto. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Goldin, Andrea Paula. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Minces, Luciana. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Chianelli, Mónica Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Keller Sarmiento, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Rosenstein, Ruth Estela. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFederation of American Societies for Experimental Biology2004-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/242110Saenz, Daniel Alberto; Goldin, Andrea Paula; Minces, Luciana; Chianelli, Mónica Silvia; Keller Sarmiento, María Inés; et al.; Effect of melatonin on the retinal glutamate/glutamine cycle in the golden hamster retina; Federation of American Societies for Experimental Biology; FASEB Journal; 18; 15; 12-2004; 1912-19130892-6638CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1096/fj.04-2062fjeinfo:eu-repo/semantics/altIdentifier/url/https://faseb.onlinelibrary.wiley.com/doi/10.1096/fj.04-2062fjeinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:16:17Zoai:ri.conicet.gov.ar:11336/242110instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:16:17.663CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of melatonin on the retinal glutamate/glutamine cycle in the golden hamster retina
title Effect of melatonin on the retinal glutamate/glutamine cycle in the golden hamster retina
spellingShingle Effect of melatonin on the retinal glutamate/glutamine cycle in the golden hamster retina
Saenz, Daniel Alberto
glutamine synthetase
L-glutamine influx
glutaminase
title_short Effect of melatonin on the retinal glutamate/glutamine cycle in the golden hamster retina
title_full Effect of melatonin on the retinal glutamate/glutamine cycle in the golden hamster retina
title_fullStr Effect of melatonin on the retinal glutamate/glutamine cycle in the golden hamster retina
title_full_unstemmed Effect of melatonin on the retinal glutamate/glutamine cycle in the golden hamster retina
title_sort Effect of melatonin on the retinal glutamate/glutamine cycle in the golden hamster retina
dc.creator.none.fl_str_mv Saenz, Daniel Alberto
Goldin, Andrea Paula
Minces, Luciana
Chianelli, Mónica Silvia
Keller Sarmiento, María Inés
Rosenstein, Ruth Estela
author Saenz, Daniel Alberto
author_facet Saenz, Daniel Alberto
Goldin, Andrea Paula
Minces, Luciana
Chianelli, Mónica Silvia
Keller Sarmiento, María Inés
Rosenstein, Ruth Estela
author_role author
author2 Goldin, Andrea Paula
Minces, Luciana
Chianelli, Mónica Silvia
Keller Sarmiento, María Inés
Rosenstein, Ruth Estela
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv glutamine synthetase
L-glutamine influx
glutaminase
topic glutamine synthetase
L-glutamine influx
glutaminase
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Glutamate is the main excitatory neurotransmitter in the retina, but it is neurotoxic when present in excessive amounts. The metabolic dependence of glutamatergic neurons upon glia via the glutamate/glutamine cycle to provide the precursor for neurotransmitter glutamate is well established. Since melatonin has been shown to be neuroprotective in several systems, in the present report, its effect on the glutamate/glutamine cycle activity was examined in the golden hamster retina. Melatonin (0.1−10 nM) significantly increased retinal glutamine synthetase activity but it did not affect L-glutamine release. A characterization of the hamster retinal Lglutamine uptake mechanism was performed. This mechanism was partly Na+ -dependent, and it was significantly inhibited by 2-aminobicyclo (2, 2, 1) heptane 2-carboxylic acid (BCH, a selective antagonists for the L-type system) and by α-(methylamino)-isobutyric acid (MeAIB, substrate characteristic for the A -type transporter) suggesting the coexistence of these transport systems in the hamster retina. Melatonin (0.1−10 nM) significantly increased total glutamine uptake as well as the BCH and the MeAIB-insensitive transporters activity. On the other hand, melatonin significantly decreased retinal glutaminase activity. On the basis of these results, it might be presumed that hamster retinal glutamate/glutamine cycle activity is regulated by physiological concentrations of melatonin. Furthermore, these findings suggest that a treatment with melatonin could be considered as a new approach to handling glutamate-mediated neuronal degeneration.
Fil: Saenz, Daniel Alberto. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Goldin, Andrea Paula. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Minces, Luciana. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Chianelli, Mónica Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Keller Sarmiento, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Rosenstein, Ruth Estela. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Glutamate is the main excitatory neurotransmitter in the retina, but it is neurotoxic when present in excessive amounts. The metabolic dependence of glutamatergic neurons upon glia via the glutamate/glutamine cycle to provide the precursor for neurotransmitter glutamate is well established. Since melatonin has been shown to be neuroprotective in several systems, in the present report, its effect on the glutamate/glutamine cycle activity was examined in the golden hamster retina. Melatonin (0.1−10 nM) significantly increased retinal glutamine synthetase activity but it did not affect L-glutamine release. A characterization of the hamster retinal Lglutamine uptake mechanism was performed. This mechanism was partly Na+ -dependent, and it was significantly inhibited by 2-aminobicyclo (2, 2, 1) heptane 2-carboxylic acid (BCH, a selective antagonists for the L-type system) and by α-(methylamino)-isobutyric acid (MeAIB, substrate characteristic for the A -type transporter) suggesting the coexistence of these transport systems in the hamster retina. Melatonin (0.1−10 nM) significantly increased total glutamine uptake as well as the BCH and the MeAIB-insensitive transporters activity. On the other hand, melatonin significantly decreased retinal glutaminase activity. On the basis of these results, it might be presumed that hamster retinal glutamate/glutamine cycle activity is regulated by physiological concentrations of melatonin. Furthermore, these findings suggest that a treatment with melatonin could be considered as a new approach to handling glutamate-mediated neuronal degeneration.
publishDate 2004
dc.date.none.fl_str_mv 2004-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/242110
Saenz, Daniel Alberto; Goldin, Andrea Paula; Minces, Luciana; Chianelli, Mónica Silvia; Keller Sarmiento, María Inés; et al.; Effect of melatonin on the retinal glutamate/glutamine cycle in the golden hamster retina; Federation of American Societies for Experimental Biology; FASEB Journal; 18; 15; 12-2004; 1912-1913
0892-6638
CONICET Digital
CONICET
url http://hdl.handle.net/11336/242110
identifier_str_mv Saenz, Daniel Alberto; Goldin, Andrea Paula; Minces, Luciana; Chianelli, Mónica Silvia; Keller Sarmiento, María Inés; et al.; Effect of melatonin on the retinal glutamate/glutamine cycle in the golden hamster retina; Federation of American Societies for Experimental Biology; FASEB Journal; 18; 15; 12-2004; 1912-1913
0892-6638
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1096/fj.04-2062fje
info:eu-repo/semantics/altIdentifier/url/https://faseb.onlinelibrary.wiley.com/doi/10.1096/fj.04-2062fje
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Federation of American Societies for Experimental Biology
publisher.none.fl_str_mv Federation of American Societies for Experimental Biology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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