Regulation of glutamine synthetase 1 and amino acids transport in the phloem of young wheat plants

Autores
Caputo Suarez, Carla Paula; Criado, Maria Victoria; Roberts, Irma; Gelso, María Alejandra; Barneix, Atilio José
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The possible regulation of amino acid remobilization via the phloem in wheat (Triticum aestivum L.) by the primary enzyme in nitrogen (N) assimilation and re-assimilation, glutamine synthetase (GS, E.C. 6.3.1.2) was studied using two conditions known to alter N phloem transport, N deficiency and cytokinins. The plants were grown for 15 days in controlled conditions with optimum N supply and then N was depleted from and/or 6-benzylaminopurine was added to the nutrient solution. Both treatments generated an induction of GS1, monitored at the level of gene expression, protein accumulation and enzyme activity, and a decrease in the exudation of amino acids to the phloem, obtained with EDTA technique, which correlated negatively. GS inhibition by metionine sulfoximide (MSX) produced an increase of amino acids exudation and the inhibitor successfully reversed the effect of N deficiency and cytokinin addition over phloem exudation. Our results point to an important physiological role for GS1 in the modulation of amino acids export levels in wheat plants. © 2009 Elsevier Masson SAS. All rights reserved.
Fil: Caputo Suarez, Carla Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina
Fil: Criado, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina
Fil: Roberts, Irma. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina
Fil: Gelso, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina
Fil: Barneix, Atilio José. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina
Materia
Amino Acids
Bap
Glutamine Synthetase
N Deficiency
Phloem Transport
Triticum Aestivum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/60884
Acceder
id CONICETDig_0c9f761cf8110cae5dc282956077bf6a
oai_identifier_str oai:ri.conicet.gov.ar:11336/60884
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Regulation of glutamine synthetase 1 and amino acids transport in the phloem of young wheat plantsCaputo Suarez, Carla PaulaCriado, Maria VictoriaRoberts, IrmaGelso, María AlejandraBarneix, Atilio JoséAmino AcidsBapGlutamine SynthetaseN DeficiencyPhloem TransportTriticum Aestivumhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The possible regulation of amino acid remobilization via the phloem in wheat (Triticum aestivum L.) by the primary enzyme in nitrogen (N) assimilation and re-assimilation, glutamine synthetase (GS, E.C. 6.3.1.2) was studied using two conditions known to alter N phloem transport, N deficiency and cytokinins. The plants were grown for 15 days in controlled conditions with optimum N supply and then N was depleted from and/or 6-benzylaminopurine was added to the nutrient solution. Both treatments generated an induction of GS1, monitored at the level of gene expression, protein accumulation and enzyme activity, and a decrease in the exudation of amino acids to the phloem, obtained with EDTA technique, which correlated negatively. GS inhibition by metionine sulfoximide (MSX) produced an increase of amino acids exudation and the inhibitor successfully reversed the effect of N deficiency and cytokinin addition over phloem exudation. Our results point to an important physiological role for GS1 in the modulation of amino acids export levels in wheat plants. © 2009 Elsevier Masson SAS. All rights reserved.Fil: Caputo Suarez, Carla Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; ArgentinaFil: Criado, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; ArgentinaFil: Roberts, Irma. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; ArgentinaFil: Gelso, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; ArgentinaFil: Barneix, Atilio José. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; ArgentinaElsevier France-editions Scientifiques Medicales Elsevier2009-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/60884Caputo Suarez, Carla Paula; Criado, Maria Victoria; Roberts, Irma; Gelso, María Alejandra; Barneix, Atilio José; Regulation of glutamine synthetase 1 and amino acids transport in the phloem of young wheat plants; Elsevier France-editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 47; 5; 5-2009; 335-3420981-9428CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.plaphy.2009.01.003info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0981942809000369info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:08:15Zoai:ri.conicet.gov.ar:11336/60884instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:08:15.775CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Regulation of glutamine synthetase 1 and amino acids transport in the phloem of young wheat plants
title Regulation of glutamine synthetase 1 and amino acids transport in the phloem of young wheat plants
spellingShingle Regulation of glutamine synthetase 1 and amino acids transport in the phloem of young wheat plants
Caputo Suarez, Carla Paula
Amino Acids
Bap
Glutamine Synthetase
N Deficiency
Phloem Transport
Triticum Aestivum
title_short Regulation of glutamine synthetase 1 and amino acids transport in the phloem of young wheat plants
title_full Regulation of glutamine synthetase 1 and amino acids transport in the phloem of young wheat plants
title_fullStr Regulation of glutamine synthetase 1 and amino acids transport in the phloem of young wheat plants
title_full_unstemmed Regulation of glutamine synthetase 1 and amino acids transport in the phloem of young wheat plants
title_sort Regulation of glutamine synthetase 1 and amino acids transport in the phloem of young wheat plants
dc.creator.none.fl_str_mv Caputo Suarez, Carla Paula
Criado, Maria Victoria
Roberts, Irma
Gelso, María Alejandra
Barneix, Atilio José
author Caputo Suarez, Carla Paula
author_facet Caputo Suarez, Carla Paula
Criado, Maria Victoria
Roberts, Irma
Gelso, María Alejandra
Barneix, Atilio José
author_role author
author2 Criado, Maria Victoria
Roberts, Irma
Gelso, María Alejandra
Barneix, Atilio José
author2_role author
author
author
author
dc.subject.none.fl_str_mv Amino Acids
Bap
Glutamine Synthetase
N Deficiency
Phloem Transport
Triticum Aestivum
topic Amino Acids
Bap
Glutamine Synthetase
N Deficiency
Phloem Transport
Triticum Aestivum
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The possible regulation of amino acid remobilization via the phloem in wheat (Triticum aestivum L.) by the primary enzyme in nitrogen (N) assimilation and re-assimilation, glutamine synthetase (GS, E.C. 6.3.1.2) was studied using two conditions known to alter N phloem transport, N deficiency and cytokinins. The plants were grown for 15 days in controlled conditions with optimum N supply and then N was depleted from and/or 6-benzylaminopurine was added to the nutrient solution. Both treatments generated an induction of GS1, monitored at the level of gene expression, protein accumulation and enzyme activity, and a decrease in the exudation of amino acids to the phloem, obtained with EDTA technique, which correlated negatively. GS inhibition by metionine sulfoximide (MSX) produced an increase of amino acids exudation and the inhibitor successfully reversed the effect of N deficiency and cytokinin addition over phloem exudation. Our results point to an important physiological role for GS1 in the modulation of amino acids export levels in wheat plants. © 2009 Elsevier Masson SAS. All rights reserved.
Fil: Caputo Suarez, Carla Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina
Fil: Criado, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina
Fil: Roberts, Irma. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina
Fil: Gelso, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina
Fil: Barneix, Atilio José. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina
description The possible regulation of amino acid remobilization via the phloem in wheat (Triticum aestivum L.) by the primary enzyme in nitrogen (N) assimilation and re-assimilation, glutamine synthetase (GS, E.C. 6.3.1.2) was studied using two conditions known to alter N phloem transport, N deficiency and cytokinins. The plants were grown for 15 days in controlled conditions with optimum N supply and then N was depleted from and/or 6-benzylaminopurine was added to the nutrient solution. Both treatments generated an induction of GS1, monitored at the level of gene expression, protein accumulation and enzyme activity, and a decrease in the exudation of amino acids to the phloem, obtained with EDTA technique, which correlated negatively. GS inhibition by metionine sulfoximide (MSX) produced an increase of amino acids exudation and the inhibitor successfully reversed the effect of N deficiency and cytokinin addition over phloem exudation. Our results point to an important physiological role for GS1 in the modulation of amino acids export levels in wheat plants. © 2009 Elsevier Masson SAS. All rights reserved.
publishDate 2009
dc.date.none.fl_str_mv 2009-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/60884
Caputo Suarez, Carla Paula; Criado, Maria Victoria; Roberts, Irma; Gelso, María Alejandra; Barneix, Atilio José; Regulation of glutamine synthetase 1 and amino acids transport in the phloem of young wheat plants; Elsevier France-editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 47; 5; 5-2009; 335-342
0981-9428
CONICET Digital
CONICET
url http://hdl.handle.net/11336/60884
identifier_str_mv Caputo Suarez, Carla Paula; Criado, Maria Victoria; Roberts, Irma; Gelso, María Alejandra; Barneix, Atilio José; Regulation of glutamine synthetase 1 and amino acids transport in the phloem of young wheat plants; Elsevier France-editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 47; 5; 5-2009; 335-342
0981-9428
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plaphy.2009.01.003
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0981942809000369
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier France-editions Scientifiques Medicales Elsevier
publisher.none.fl_str_mv Elsevier France-editions Scientifiques Medicales Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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