Synthesis and characterization of novel scaffold for bone tissue engineering based on Whartons´s jelly

Autores
Martinez, Cristian; Fernández, Carlos; Prado, Miguel Oscar; Ozols, Andres; Olmedo, Daniel Gustavo
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A composite is a material made of more than one component, and the bond between the components is on a scale larger than the atomic scale. The objective of the present study was to synthesize and perform the structural characterization and biological evaluation of a new biocomposite (BCO) based on a novel combination of an organic and an inorganic phase, for bone tissue engineering applications. The organic phase consisted of Wharton´s Jelly (WJ), which was obtained from embryonic tissue following a protocol developed by our laboratory. The inorganic phase consisted of bioceramic particles (BC), produced by sintering hydroxyapatite (HA) with β- tricalcium phosphate (β-TCP), and bioactive glass particles (BG). Each phase of the BCO was fully characterized by SEM, EDS, XRD and FTIR. Biocompatibility was evaluated in vivo in the tibiae of Wistar rats (n=40). Histological evaluation was performed at 0, 1, 7, 14, 30 and 60 days. XRD showed the phases corresponding to HA and β-TCP, whereas diffractogram of BG showed it to have an amorphous structure. EDS showed mainly Si and Na, Ca, P in BG, and Ca and P in HA and β-TCP. FTIR identified bonds between the organic and inorganic phases. From a mechanical viewpoint, the composite showed high flexural strength of 40.3±0.8MPa. The synthesized BCO exhibited adequate biocompatibility as shown by formation of lamellar type bone linked by BG and BC particles. The biomaterial presented here showed excellent mechanical and biocompatibility properties for its potential clinical use.
Fil: Martinez, Cristian. Universidad de Buenos Aires. Facultad de Ingenieria. Instituto de Ingeniería Biomédica; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica; Argentina. Universidad Nacional de Cuyo. Facultad de Odontologia; Argentina
Fil: Fernández, Carlos. Universidad de Buenos Aires. Facultad de Ingenieria. Instituto de Ingeniería Biomédica; Argentina
Fil: Prado, Miguel Oscar. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ozols, Andres. Universidad de Buenos Aires. Facultad de Ingenieria. Instituto de Ingeniería Biomédica; Argentina
Fil: Olmedo, Daniel Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica; Argentina
Materia
BIOCOMPOSITE
BIOMATERIAL
WHARTON´S JELLY
BONE REGENERATION
BIOMIMETIC MATERIAL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47578
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/47578
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Synthesis and characterization of novel scaffold for bone tissue engineering based on Whartons´s jellyMartinez, CristianFernández, CarlosPrado, Miguel OscarOzols, AndresOlmedo, Daniel GustavoBIOCOMPOSITEBIOMATERIALWHARTON´S JELLYBONE REGENERATIONBIOMIMETIC MATERIALhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3A composite is a material made of more than one component, and the bond between the components is on a scale larger than the atomic scale. The objective of the present study was to synthesize and perform the structural characterization and biological evaluation of a new biocomposite (BCO) based on a novel combination of an organic and an inorganic phase, for bone tissue engineering applications. The organic phase consisted of Wharton´s Jelly (WJ), which was obtained from embryonic tissue following a protocol developed by our laboratory. The inorganic phase consisted of bioceramic particles (BC), produced by sintering hydroxyapatite (HA) with β- tricalcium phosphate (β-TCP), and bioactive glass particles (BG). Each phase of the BCO was fully characterized by SEM, EDS, XRD and FTIR. Biocompatibility was evaluated in vivo in the tibiae of Wistar rats (n=40). Histological evaluation was performed at 0, 1, 7, 14, 30 and 60 days. XRD showed the phases corresponding to HA and β-TCP, whereas diffractogram of BG showed it to have an amorphous structure. EDS showed mainly Si and Na, Ca, P in BG, and Ca and P in HA and β-TCP. FTIR identified bonds between the organic and inorganic phases. From a mechanical viewpoint, the composite showed high flexural strength of 40.3±0.8MPa. The synthesized BCO exhibited adequate biocompatibility as shown by formation of lamellar type bone linked by BG and BC particles. The biomaterial presented here showed excellent mechanical and biocompatibility properties for its potential clinical use.Fil: Martinez, Cristian. Universidad de Buenos Aires. Facultad de Ingenieria. Instituto de Ingeniería Biomédica; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica; Argentina. Universidad Nacional de Cuyo. Facultad de Odontologia; ArgentinaFil: Fernández, Carlos. Universidad de Buenos Aires. Facultad de Ingenieria. Instituto de Ingeniería Biomédica; ArgentinaFil: Prado, Miguel Oscar. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ozols, Andres. Universidad de Buenos Aires. Facultad de Ingenieria. Instituto de Ingeniería Biomédica; ArgentinaFil: Olmedo, Daniel Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica; ArgentinaWiley-liss, Div John Wiley & Sons Inc2017-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47578Martinez, Cristian; Fernández, Carlos; Prado, Miguel Oscar; Ozols, Andres; Olmedo, Daniel Gustavo; Synthesis and characterization of novel scaffold for bone tissue engineering based on Whartons´s jelly; Wiley-liss, Div John Wiley & Sons Inc; Journal of Biomedical Materials Research Part A; 105; 4; 4-2017; 1034-10451549-32961552-4965CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/jbm.a.35976info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jbm.a.35976info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:18:38Zoai:ri.conicet.gov.ar:11336/47578instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:18:39.165CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Synthesis and characterization of novel scaffold for bone tissue engineering based on Whartons´s jelly
title Synthesis and characterization of novel scaffold for bone tissue engineering based on Whartons´s jelly
spellingShingle Synthesis and characterization of novel scaffold for bone tissue engineering based on Whartons´s jelly
Martinez, Cristian
BIOCOMPOSITE
BIOMATERIAL
WHARTON´S JELLY
BONE REGENERATION
BIOMIMETIC MATERIAL
title_short Synthesis and characterization of novel scaffold for bone tissue engineering based on Whartons´s jelly
title_full Synthesis and characterization of novel scaffold for bone tissue engineering based on Whartons´s jelly
title_fullStr Synthesis and characterization of novel scaffold for bone tissue engineering based on Whartons´s jelly
title_full_unstemmed Synthesis and characterization of novel scaffold for bone tissue engineering based on Whartons´s jelly
title_sort Synthesis and characterization of novel scaffold for bone tissue engineering based on Whartons´s jelly
dc.creator.none.fl_str_mv Martinez, Cristian
Fernández, Carlos
Prado, Miguel Oscar
Ozols, Andres
Olmedo, Daniel Gustavo
author Martinez, Cristian
author_facet Martinez, Cristian
Fernández, Carlos
Prado, Miguel Oscar
Ozols, Andres
Olmedo, Daniel Gustavo
author_role author
author2 Fernández, Carlos
Prado, Miguel Oscar
Ozols, Andres
Olmedo, Daniel Gustavo
author2_role author
author
author
author
dc.subject.none.fl_str_mv BIOCOMPOSITE
BIOMATERIAL
WHARTON´S JELLY
BONE REGENERATION
BIOMIMETIC MATERIAL
topic BIOCOMPOSITE
BIOMATERIAL
WHARTON´S JELLY
BONE REGENERATION
BIOMIMETIC MATERIAL
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv A composite is a material made of more than one component, and the bond between the components is on a scale larger than the atomic scale. The objective of the present study was to synthesize and perform the structural characterization and biological evaluation of a new biocomposite (BCO) based on a novel combination of an organic and an inorganic phase, for bone tissue engineering applications. The organic phase consisted of Wharton´s Jelly (WJ), which was obtained from embryonic tissue following a protocol developed by our laboratory. The inorganic phase consisted of bioceramic particles (BC), produced by sintering hydroxyapatite (HA) with β- tricalcium phosphate (β-TCP), and bioactive glass particles (BG). Each phase of the BCO was fully characterized by SEM, EDS, XRD and FTIR. Biocompatibility was evaluated in vivo in the tibiae of Wistar rats (n=40). Histological evaluation was performed at 0, 1, 7, 14, 30 and 60 days. XRD showed the phases corresponding to HA and β-TCP, whereas diffractogram of BG showed it to have an amorphous structure. EDS showed mainly Si and Na, Ca, P in BG, and Ca and P in HA and β-TCP. FTIR identified bonds between the organic and inorganic phases. From a mechanical viewpoint, the composite showed high flexural strength of 40.3±0.8MPa. The synthesized BCO exhibited adequate biocompatibility as shown by formation of lamellar type bone linked by BG and BC particles. The biomaterial presented here showed excellent mechanical and biocompatibility properties for its potential clinical use.
Fil: Martinez, Cristian. Universidad de Buenos Aires. Facultad de Ingenieria. Instituto de Ingeniería Biomédica; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica; Argentina. Universidad Nacional de Cuyo. Facultad de Odontologia; Argentina
Fil: Fernández, Carlos. Universidad de Buenos Aires. Facultad de Ingenieria. Instituto de Ingeniería Biomédica; Argentina
Fil: Prado, Miguel Oscar. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ozols, Andres. Universidad de Buenos Aires. Facultad de Ingenieria. Instituto de Ingeniería Biomédica; Argentina
Fil: Olmedo, Daniel Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica; Argentina
description A composite is a material made of more than one component, and the bond between the components is on a scale larger than the atomic scale. The objective of the present study was to synthesize and perform the structural characterization and biological evaluation of a new biocomposite (BCO) based on a novel combination of an organic and an inorganic phase, for bone tissue engineering applications. The organic phase consisted of Wharton´s Jelly (WJ), which was obtained from embryonic tissue following a protocol developed by our laboratory. The inorganic phase consisted of bioceramic particles (BC), produced by sintering hydroxyapatite (HA) with β- tricalcium phosphate (β-TCP), and bioactive glass particles (BG). Each phase of the BCO was fully characterized by SEM, EDS, XRD and FTIR. Biocompatibility was evaluated in vivo in the tibiae of Wistar rats (n=40). Histological evaluation was performed at 0, 1, 7, 14, 30 and 60 days. XRD showed the phases corresponding to HA and β-TCP, whereas diffractogram of BG showed it to have an amorphous structure. EDS showed mainly Si and Na, Ca, P in BG, and Ca and P in HA and β-TCP. FTIR identified bonds between the organic and inorganic phases. From a mechanical viewpoint, the composite showed high flexural strength of 40.3±0.8MPa. The synthesized BCO exhibited adequate biocompatibility as shown by formation of lamellar type bone linked by BG and BC particles. The biomaterial presented here showed excellent mechanical and biocompatibility properties for its potential clinical use.
publishDate 2017
dc.date.none.fl_str_mv 2017-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47578
Martinez, Cristian; Fernández, Carlos; Prado, Miguel Oscar; Ozols, Andres; Olmedo, Daniel Gustavo; Synthesis and characterization of novel scaffold for bone tissue engineering based on Whartons´s jelly; Wiley-liss, Div John Wiley & Sons Inc; Journal of Biomedical Materials Research Part A; 105; 4; 4-2017; 1034-1045
1549-3296
1552-4965
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47578
identifier_str_mv Martinez, Cristian; Fernández, Carlos; Prado, Miguel Oscar; Ozols, Andres; Olmedo, Daniel Gustavo; Synthesis and characterization of novel scaffold for bone tissue engineering based on Whartons´s jelly; Wiley-liss, Div John Wiley & Sons Inc; Journal of Biomedical Materials Research Part A; 105; 4; 4-2017; 1034-1045
1549-3296
1552-4965
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/jbm.a.35976
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jbm.a.35976
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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