Bone Regeneration with Wharton's Jelly-Bioceramic-Bioglass Composite

Autores
Fernández, C.A.; Martínez, C.A.; Prado, Miguel Oscar; Olmedo, Daniel Gustavo; Ozols, Andres
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this development is to optimize a bone substitute (BS) for use in tissue engineering. This is achieved through the combination of three phases in a biocomposite (BCO), in which each is reabsorbed in the site of implantation and replaced by autologous bone (patient´s own). The inorganic phases are composed of irregular particles (150-300 microns) obtained by milling and sieving of a biphasic bioceramic (BC) of hydroxyapatite (HA of bovine origin) with 40% (wt.) β-tricalcium phosphate (β- TCP, obtained by chemical synthesis) and Bioglass type 45S5 (45SiO2 -24,5CaO - 24,5Na2O - 6P2O5, in % wt.). Instead, the organic phase consists of collagen extracted from Wharton´s jelly (part of the human embryonic tissue) from physical and chemical self-developed process. The BC is produced by mixture of HA and β-TCP (< 45 μm) and molding by gelcasting with albumin in aqueous solutions, drying and sintering at 1200 °C for 2 hours. The BG is obtained from the mixture of the oxides, melting at 1350 °C and cast onto metal. Each phase and BCO is subjected to studies by electron microscopy (SEM and EDS), X-ray diffraction (DRX) and infrared spectrometry (FT-IR). The biocompatibility is evaluated by in vivo studies using the laminar implant model in Wistar rats (n=40). Histological samples show high biocompatibility and ability to integrate with the bone tissue. 30 days after implantation, the material is completely reabsorbed and the bone regeneration process starts, the primary objective. The process developed allows the synthesis of a new BS with excellent biological properties for clinical use.
Bone Regeneration with Wharton´s Jelly-Bioceramic-Bioglass Composite. Available from: 
Fil: Fernández, C.A.. Universidad de Buenos Aires. Facultad de Ingeniería. Departamento de Química; Argentina
Fil: Martínez, C.A.. Universidad de Buenos Aires. Facultad de Ingeniería. Departamento de Química; Argentina. Universidad Nacional de Cuyo. Facultad de Odontologia; Argentina
Fil: Prado, Miguel Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Comision Nacional de Energia Atomica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Investigación Aplicada. Grupo de Materiales Nucleares; Argentina
Fil: Olmedo, Daniel Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Ozols, Andres. Universidad de Buenos Aires. Facultad de Ingeniería. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Materia
Biocomposite
Tissue engineering
Bioceramics
Biogallss
Wharton Jelly
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/114198
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/114198
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bone Regeneration with Wharton's Jelly-Bioceramic-Bioglass CompositeFernández, C.A.Martínez, C.A.Prado, Miguel OscarOlmedo, Daniel GustavoOzols, AndresBiocompositeTissue engineeringBioceramicsBiogallssWharton Jellyhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3The aim of this development is to optimize a bone substitute (BS) for use in tissue engineering. This is achieved through the combination of three phases in a biocomposite (BCO), in which each is reabsorbed in the site of implantation and replaced by autologous bone (patient´s own). The inorganic phases are composed of irregular particles (150-300 microns) obtained by milling and sieving of a biphasic bioceramic (BC) of hydroxyapatite (HA of bovine origin) with 40% (wt.) β-tricalcium phosphate (β- TCP, obtained by chemical synthesis) and Bioglass type 45S5 (45SiO2 -24,5CaO - 24,5Na2O - 6P2O5, in % wt.). Instead, the organic phase consists of collagen extracted from Wharton´s jelly (part of the human embryonic tissue) from physical and chemical self-developed process. The BC is produced by mixture of HA and β-TCP (< 45 μm) and molding by gelcasting with albumin in aqueous solutions, drying and sintering at 1200 °C for 2 hours. The BG is obtained from the mixture of the oxides, melting at 1350 °C and cast onto metal. Each phase and BCO is subjected to studies by electron microscopy (SEM and EDS), X-ray diffraction (DRX) and infrared spectrometry (FT-IR). The biocompatibility is evaluated by in vivo studies using the laminar implant model in Wistar rats (n=40). Histological samples show high biocompatibility and ability to integrate with the bone tissue. 30 days after implantation, the material is completely reabsorbed and the bone regeneration process starts, the primary objective. The process developed allows the synthesis of a new BS with excellent biological properties for clinical use.<br /><i>Bone Regeneration with Wharton´s Jelly-Bioceramic-Bioglass Composite</i>. Available from: Fil: Fernández, C.A.. Universidad de Buenos Aires. Facultad de Ingeniería. Departamento de Química; ArgentinaFil: Martínez, C.A.. Universidad de Buenos Aires. Facultad de Ingeniería. Departamento de Química; Argentina. Universidad Nacional de Cuyo. Facultad de Odontologia; ArgentinaFil: Prado, Miguel Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Comision Nacional de Energia Atomica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Investigación Aplicada. Grupo de Materiales Nucleares; ArgentinaFil: Olmedo, Daniel Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Ozols, Andres. Universidad de Buenos Aires. Facultad de Ingeniería. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaElsevier2015-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/114198Fernández, C.A.; Martínez, C.A.; Prado, Miguel Oscar; Olmedo, Daniel Gustavo; Ozols, Andres; Bone Regeneration with Wharton's Jelly-Bioceramic-Bioglass Composite; Elsevier; Procedia Materials Science; 9; 12-2015; 205-2122211-8128CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2211812815000279?via%3Dihubinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.mspro.2015.04.026info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:41Zoai:ri.conicet.gov.ar:11336/114198instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:41.841CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bone Regeneration with Wharton's Jelly-Bioceramic-Bioglass Composite
title Bone Regeneration with Wharton's Jelly-Bioceramic-Bioglass Composite
spellingShingle Bone Regeneration with Wharton's Jelly-Bioceramic-Bioglass Composite
Fernández, C.A.
Biocomposite
Tissue engineering
Bioceramics
Biogallss
Wharton Jelly
title_short Bone Regeneration with Wharton's Jelly-Bioceramic-Bioglass Composite
title_full Bone Regeneration with Wharton's Jelly-Bioceramic-Bioglass Composite
title_fullStr Bone Regeneration with Wharton's Jelly-Bioceramic-Bioglass Composite
title_full_unstemmed Bone Regeneration with Wharton's Jelly-Bioceramic-Bioglass Composite
title_sort Bone Regeneration with Wharton's Jelly-Bioceramic-Bioglass Composite
dc.creator.none.fl_str_mv Fernández, C.A.
Martínez, C.A.
Prado, Miguel Oscar
Olmedo, Daniel Gustavo
Ozols, Andres
author Fernández, C.A.
author_facet Fernández, C.A.
Martínez, C.A.
Prado, Miguel Oscar
Olmedo, Daniel Gustavo
Ozols, Andres
author_role author
author2 Martínez, C.A.
Prado, Miguel Oscar
Olmedo, Daniel Gustavo
Ozols, Andres
author2_role author
author
author
author
dc.subject.none.fl_str_mv Biocomposite
Tissue engineering
Bioceramics
Biogallss
Wharton Jelly
topic Biocomposite
Tissue engineering
Bioceramics
Biogallss
Wharton Jelly
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The aim of this development is to optimize a bone substitute (BS) for use in tissue engineering. This is achieved through the combination of three phases in a biocomposite (BCO), in which each is reabsorbed in the site of implantation and replaced by autologous bone (patient´s own). The inorganic phases are composed of irregular particles (150-300 microns) obtained by milling and sieving of a biphasic bioceramic (BC) of hydroxyapatite (HA of bovine origin) with 40% (wt.) β-tricalcium phosphate (β- TCP, obtained by chemical synthesis) and Bioglass type 45S5 (45SiO2 -24,5CaO - 24,5Na2O - 6P2O5, in % wt.). Instead, the organic phase consists of collagen extracted from Wharton´s jelly (part of the human embryonic tissue) from physical and chemical self-developed process. The BC is produced by mixture of HA and β-TCP (< 45 μm) and molding by gelcasting with albumin in aqueous solutions, drying and sintering at 1200 °C for 2 hours. The BG is obtained from the mixture of the oxides, melting at 1350 °C and cast onto metal. Each phase and BCO is subjected to studies by electron microscopy (SEM and EDS), X-ray diffraction (DRX) and infrared spectrometry (FT-IR). The biocompatibility is evaluated by in vivo studies using the laminar implant model in Wistar rats (n=40). Histological samples show high biocompatibility and ability to integrate with the bone tissue. 30 days after implantation, the material is completely reabsorbed and the bone regeneration process starts, the primary objective. The process developed allows the synthesis of a new BS with excellent biological properties for clinical use.<br /><i>Bone Regeneration with Wharton´s Jelly-Bioceramic-Bioglass Composite</i>. Available from: 
Fil: Fernández, C.A.. Universidad de Buenos Aires. Facultad de Ingeniería. Departamento de Química; Argentina
Fil: Martínez, C.A.. Universidad de Buenos Aires. Facultad de Ingeniería. Departamento de Química; Argentina. Universidad Nacional de Cuyo. Facultad de Odontologia; Argentina
Fil: Prado, Miguel Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Comision Nacional de Energia Atomica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Investigación Aplicada. Grupo de Materiales Nucleares; Argentina
Fil: Olmedo, Daniel Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Ozols, Andres. Universidad de Buenos Aires. Facultad de Ingeniería. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
description The aim of this development is to optimize a bone substitute (BS) for use in tissue engineering. This is achieved through the combination of three phases in a biocomposite (BCO), in which each is reabsorbed in the site of implantation and replaced by autologous bone (patient´s own). The inorganic phases are composed of irregular particles (150-300 microns) obtained by milling and sieving of a biphasic bioceramic (BC) of hydroxyapatite (HA of bovine origin) with 40% (wt.) β-tricalcium phosphate (β- TCP, obtained by chemical synthesis) and Bioglass type 45S5 (45SiO2 -24,5CaO - 24,5Na2O - 6P2O5, in % wt.). Instead, the organic phase consists of collagen extracted from Wharton´s jelly (part of the human embryonic tissue) from physical and chemical self-developed process. The BC is produced by mixture of HA and β-TCP (< 45 μm) and molding by gelcasting with albumin in aqueous solutions, drying and sintering at 1200 °C for 2 hours. The BG is obtained from the mixture of the oxides, melting at 1350 °C and cast onto metal. Each phase and BCO is subjected to studies by electron microscopy (SEM and EDS), X-ray diffraction (DRX) and infrared spectrometry (FT-IR). The biocompatibility is evaluated by in vivo studies using the laminar implant model in Wistar rats (n=40). Histological samples show high biocompatibility and ability to integrate with the bone tissue. 30 days after implantation, the material is completely reabsorbed and the bone regeneration process starts, the primary objective. The process developed allows the synthesis of a new BS with excellent biological properties for clinical use.<br /><i>Bone Regeneration with Wharton´s Jelly-Bioceramic-Bioglass Composite</i>. Available from: 
publishDate 2015
dc.date.none.fl_str_mv 2015-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/114198
Fernández, C.A.; Martínez, C.A.; Prado, Miguel Oscar; Olmedo, Daniel Gustavo; Ozols, Andres; Bone Regeneration with Wharton's Jelly-Bioceramic-Bioglass Composite; Elsevier; Procedia Materials Science; 9; 12-2015; 205-212
2211-8128
CONICET Digital
CONICET
url http://hdl.handle.net/11336/114198
identifier_str_mv Fernández, C.A.; Martínez, C.A.; Prado, Miguel Oscar; Olmedo, Daniel Gustavo; Ozols, Andres; Bone Regeneration with Wharton's Jelly-Bioceramic-Bioglass Composite; Elsevier; Procedia Materials Science; 9; 12-2015; 205-212
2211-8128
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2211812815000279?via%3Dihub
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mspro.2015.04.026
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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