Tissue response to porous high density polyethylene as a three-dimensional scaffold for bone tissue engineering: An experimental study

Autores
Martínez Rodríguez, Juliana; Renou, Sandra Judith; Guglielmotti, Maria Beatriz; Olmedo, Daniel Gustavo
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
High density polyethylene (HDPE) is a synthetic biomaterial used as a three-dimensional scaffold for bone defect reconstruction. Reports differ with regard to its biological response, particularly its osteoconductive capacity. The aim of the present work was to histologically and histomorphometrically evaluate tissue response to porous HDPE. An in vivo study was conducted in rat tibia to evaluate osteogenic capacity, angiogenesis, inflammatory response, and the presence of multinucleated giant cells 14 and 60 days post-biomaterial implantation. Histological examination 14 days post-implantation showed fibrovascular tissue inside pores and on the surface of porous HDPE, acute inflammatory response, scant multinucleated giant cells (MNGCs), and lamellar bone in contact with the biomaterial. An increase in the proportion of lamellar bone tissue, no inflammatory response, and a decrease in the number of MNGCs were observed at 60 days. The histomorphometric study showed a significant time-dependent increase both in the area of bone tissue formed in contact with the porous HDPE (14d: 24.450 ± 11.623 µm 2 vs. 60d: 77.104 ± 26.217 µm 2 , p < 0.05) and in the percentage of bone tissue in contact with the porous HDPE (osseointegration). A significant decrease in the number of MNGCs was also observed at 60 days post-implantation. Porous HDPE showed adequate osteoconductive properties, and only caused an initial inflammatory response. Although this biomaterial has traditionally been used juxtaosseoulsy, its adequate osteoconductive properties broaden the scope of its application to include intraosseous placement.
Fil: Martínez Rodríguez, Juliana. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Renou, Sandra Judith. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Guglielmotti, Maria Beatriz. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Olmedo, Daniel Gustavo. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Materia
BIOMATERIALS
BONE REGENERATION
OSTEOGENESIS
POROUS HIGH DENSITY POLYETHYLENE
THREE-DIMENSIONAL SCAFFOLDS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/134872
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/134872
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Tissue response to porous high density polyethylene as a three-dimensional scaffold for bone tissue engineering: An experimental studyMartínez Rodríguez, JulianaRenou, Sandra JudithGuglielmotti, Maria BeatrizOlmedo, Daniel GustavoBIOMATERIALSBONE REGENERATIONOSTEOGENESISPOROUS HIGH DENSITY POLYETHYLENETHREE-DIMENSIONAL SCAFFOLDShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3High density polyethylene (HDPE) is a synthetic biomaterial used as a three-dimensional scaffold for bone defect reconstruction. Reports differ with regard to its biological response, particularly its osteoconductive capacity. The aim of the present work was to histologically and histomorphometrically evaluate tissue response to porous HDPE. An in vivo study was conducted in rat tibia to evaluate osteogenic capacity, angiogenesis, inflammatory response, and the presence of multinucleated giant cells 14 and 60 days post-biomaterial implantation. Histological examination 14 days post-implantation showed fibrovascular tissue inside pores and on the surface of porous HDPE, acute inflammatory response, scant multinucleated giant cells (MNGCs), and lamellar bone in contact with the biomaterial. An increase in the proportion of lamellar bone tissue, no inflammatory response, and a decrease in the number of MNGCs were observed at 60 days. The histomorphometric study showed a significant time-dependent increase both in the area of bone tissue formed in contact with the porous HDPE (14d: 24.450 ± 11.623 µm 2 vs. 60d: 77.104 ± 26.217 µm 2 , p < 0.05) and in the percentage of bone tissue in contact with the porous HDPE (osseointegration). A significant decrease in the number of MNGCs was also observed at 60 days post-implantation. Porous HDPE showed adequate osteoconductive properties, and only caused an initial inflammatory response. Although this biomaterial has traditionally been used juxtaosseoulsy, its adequate osteoconductive properties broaden the scope of its application to include intraosseous placement.Fil: Martínez Rodríguez, Juliana. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Renou, Sandra Judith. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Guglielmotti, Maria Beatriz. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Olmedo, Daniel Gustavo. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaVsp Bv2019-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/134872Martínez Rodríguez, Juliana; Renou, Sandra Judith; Guglielmotti, Maria Beatriz; Olmedo, Daniel Gustavo; Tissue response to porous high density polyethylene as a three-dimensional scaffold for bone tissue engineering: An experimental study; Vsp Bv; Journal of Biomaterials Science - Polymer Edition; 30; 6; 4-2019; 486-4990920-5063CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/09205063.2019.1582278info:eu-repo/semantics/altIdentifier/doi/10.1080/09205063.2019.1582278info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:33:12Zoai:ri.conicet.gov.ar:11336/134872instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:33:12.425CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Tissue response to porous high density polyethylene as a three-dimensional scaffold for bone tissue engineering: An experimental study
title Tissue response to porous high density polyethylene as a three-dimensional scaffold for bone tissue engineering: An experimental study
spellingShingle Tissue response to porous high density polyethylene as a three-dimensional scaffold for bone tissue engineering: An experimental study
Martínez Rodríguez, Juliana
BIOMATERIALS
BONE REGENERATION
OSTEOGENESIS
POROUS HIGH DENSITY POLYETHYLENE
THREE-DIMENSIONAL SCAFFOLDS
title_short Tissue response to porous high density polyethylene as a three-dimensional scaffold for bone tissue engineering: An experimental study
title_full Tissue response to porous high density polyethylene as a three-dimensional scaffold for bone tissue engineering: An experimental study
title_fullStr Tissue response to porous high density polyethylene as a three-dimensional scaffold for bone tissue engineering: An experimental study
title_full_unstemmed Tissue response to porous high density polyethylene as a three-dimensional scaffold for bone tissue engineering: An experimental study
title_sort Tissue response to porous high density polyethylene as a three-dimensional scaffold for bone tissue engineering: An experimental study
dc.creator.none.fl_str_mv Martínez Rodríguez, Juliana
Renou, Sandra Judith
Guglielmotti, Maria Beatriz
Olmedo, Daniel Gustavo
author Martínez Rodríguez, Juliana
author_facet Martínez Rodríguez, Juliana
Renou, Sandra Judith
Guglielmotti, Maria Beatriz
Olmedo, Daniel Gustavo
author_role author
author2 Renou, Sandra Judith
Guglielmotti, Maria Beatriz
Olmedo, Daniel Gustavo
author2_role author
author
author
dc.subject.none.fl_str_mv BIOMATERIALS
BONE REGENERATION
OSTEOGENESIS
POROUS HIGH DENSITY POLYETHYLENE
THREE-DIMENSIONAL SCAFFOLDS
topic BIOMATERIALS
BONE REGENERATION
OSTEOGENESIS
POROUS HIGH DENSITY POLYETHYLENE
THREE-DIMENSIONAL SCAFFOLDS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv High density polyethylene (HDPE) is a synthetic biomaterial used as a three-dimensional scaffold for bone defect reconstruction. Reports differ with regard to its biological response, particularly its osteoconductive capacity. The aim of the present work was to histologically and histomorphometrically evaluate tissue response to porous HDPE. An in vivo study was conducted in rat tibia to evaluate osteogenic capacity, angiogenesis, inflammatory response, and the presence of multinucleated giant cells 14 and 60 days post-biomaterial implantation. Histological examination 14 days post-implantation showed fibrovascular tissue inside pores and on the surface of porous HDPE, acute inflammatory response, scant multinucleated giant cells (MNGCs), and lamellar bone in contact with the biomaterial. An increase in the proportion of lamellar bone tissue, no inflammatory response, and a decrease in the number of MNGCs were observed at 60 days. The histomorphometric study showed a significant time-dependent increase both in the area of bone tissue formed in contact with the porous HDPE (14d: 24.450 ± 11.623 µm 2 vs. 60d: 77.104 ± 26.217 µm 2 , p < 0.05) and in the percentage of bone tissue in contact with the porous HDPE (osseointegration). A significant decrease in the number of MNGCs was also observed at 60 days post-implantation. Porous HDPE showed adequate osteoconductive properties, and only caused an initial inflammatory response. Although this biomaterial has traditionally been used juxtaosseoulsy, its adequate osteoconductive properties broaden the scope of its application to include intraosseous placement.
Fil: Martínez Rodríguez, Juliana. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Renou, Sandra Judith. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Guglielmotti, Maria Beatriz. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Olmedo, Daniel Gustavo. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
description High density polyethylene (HDPE) is a synthetic biomaterial used as a three-dimensional scaffold for bone defect reconstruction. Reports differ with regard to its biological response, particularly its osteoconductive capacity. The aim of the present work was to histologically and histomorphometrically evaluate tissue response to porous HDPE. An in vivo study was conducted in rat tibia to evaluate osteogenic capacity, angiogenesis, inflammatory response, and the presence of multinucleated giant cells 14 and 60 days post-biomaterial implantation. Histological examination 14 days post-implantation showed fibrovascular tissue inside pores and on the surface of porous HDPE, acute inflammatory response, scant multinucleated giant cells (MNGCs), and lamellar bone in contact with the biomaterial. An increase in the proportion of lamellar bone tissue, no inflammatory response, and a decrease in the number of MNGCs were observed at 60 days. The histomorphometric study showed a significant time-dependent increase both in the area of bone tissue formed in contact with the porous HDPE (14d: 24.450 ± 11.623 µm 2 vs. 60d: 77.104 ± 26.217 µm 2 , p < 0.05) and in the percentage of bone tissue in contact with the porous HDPE (osseointegration). A significant decrease in the number of MNGCs was also observed at 60 days post-implantation. Porous HDPE showed adequate osteoconductive properties, and only caused an initial inflammatory response. Although this biomaterial has traditionally been used juxtaosseoulsy, its adequate osteoconductive properties broaden the scope of its application to include intraosseous placement.
publishDate 2019
dc.date.none.fl_str_mv 2019-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/134872
Martínez Rodríguez, Juliana; Renou, Sandra Judith; Guglielmotti, Maria Beatriz; Olmedo, Daniel Gustavo; Tissue response to porous high density polyethylene as a three-dimensional scaffold for bone tissue engineering: An experimental study; Vsp Bv; Journal of Biomaterials Science - Polymer Edition; 30; 6; 4-2019; 486-499
0920-5063
CONICET Digital
CONICET
url http://hdl.handle.net/11336/134872
identifier_str_mv Martínez Rodríguez, Juliana; Renou, Sandra Judith; Guglielmotti, Maria Beatriz; Olmedo, Daniel Gustavo; Tissue response to porous high density polyethylene as a three-dimensional scaffold for bone tissue engineering: An experimental study; Vsp Bv; Journal of Biomaterials Science - Polymer Edition; 30; 6; 4-2019; 486-499
0920-5063
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/09205063.2019.1582278
info:eu-repo/semantics/altIdentifier/doi/10.1080/09205063.2019.1582278
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Vsp Bv
publisher.none.fl_str_mv Vsp Bv
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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