Synthetic Three-Dimensional Scaffold for Application in the Regeneration of Bone Tissue

Autores
Coletta, Dante Jesus; Missana, Liliana Raquel; Martins, Talita; Jammal, María Victoria; García, Luciano Andres; Farez, Nayla; Glee, Tomas De; Paulo Mardegan Issa, João; Feldman, Sara
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bone tissue engineering aims to use biodegrade.able scaffolds to replace damaged tissue. This scaffold must be gradually degraded and replaced by tissue as similar as possible to the original one. In this work a hybrid porous scaffold containing chitosan, polyvinyl alcohol and bioactive glass was suc-cessfully obtained and subsequently characterized by scanning electron mi-croscopy. The scaffold presented satisfactory pore size range and open inter-connected pores, which are essential for tissue ingrowth. A cytotoxicity assay showed that this biomaterial allows adequate cell viability, so that it was con-sidered suitable for an in vivo experiment. Promising resuts were obtained with the implant of the scaffold in an experimental model of a New Zealand rabbit femur bone lesion. Clinical and biochemical parameters measured such as complete blood count, total serum proteins, albumin, alanine ami-notransferase and aspartate aminotransferase were similar between animals in the control group at all time periods studied. Histological and histometric studies showed that the scaffold was coated with a cement-like substance, exhibiting many areas of mineralized structures. Very few osteocyte-like cells or lining-like cells were found inside the amorphous mineralized deposit. In vivo results allow us to consider this scaffold as a promising biomaterial to be applied in bone tissue engineering.
Fil: Coletta, Dante Jesus. Universidad Nacional de Rosario. Facultad de Cs.medicas. Laboratorio de Biologia Osteoarticular, Ingenieria Tisular y Terapias Emergentes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnol.conicet - Rosario. Unidad de Direccion; Argentina
Fil: Missana, Liliana Raquel. Okayama University; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Martins, Talita. Universidade Federal de Minas Gerais; Brasil
Fil: Jammal, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: García, Luciano Andres. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Osteoarticular, Ingeniería Tisular y Terapias Emergentes; Argentina
Fil: Farez, Nayla. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Osteoarticular, Ingeniería Tisular y Terapias Emergentes; Argentina
Fil: Glee, Tomas De. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Osteoarticular, Ingeniería Tisular y Terapias Emergentes; Argentina
Fil: Paulo Mardegan Issa, João. Universidade de Sao Paulo; Brasil
Fil: Feldman, Sara. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Osteoarticular, Ingeniería Tisular y Terapias Emergentes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnol.conicet - Rosario. Unidad de Direccion; Argentina
Materia
BIOMATERIAL SCAFFOLD
BONE
CHITOSAN
POLY(VINYL ALCOHOL-CO-VINYL ACETATE)
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/91947
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/91947
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Synthetic Three-Dimensional Scaffold for Application in the Regeneration of Bone TissueColetta, Dante JesusMissana, Liliana RaquelMartins, TalitaJammal, María VictoriaGarcía, Luciano AndresFarez, NaylaGlee, Tomas DePaulo Mardegan Issa, JoãoFeldman, SaraBIOMATERIAL SCAFFOLDBONECHITOSANPOLY(VINYL ALCOHOL-CO-VINYL ACETATE)https://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Bone tissue engineering aims to use biodegrade.able scaffolds to replace damaged tissue. This scaffold must be gradually degraded and replaced by tissue as similar as possible to the original one. In this work a hybrid porous scaffold containing chitosan, polyvinyl alcohol and bioactive glass was suc-cessfully obtained and subsequently characterized by scanning electron mi-croscopy. The scaffold presented satisfactory pore size range and open inter-connected pores, which are essential for tissue ingrowth. A cytotoxicity assay showed that this biomaterial allows adequate cell viability, so that it was con-sidered suitable for an in vivo experiment. Promising resuts were obtained with the implant of the scaffold in an experimental model of a New Zealand rabbit femur bone lesion. Clinical and biochemical parameters measured such as complete blood count, total serum proteins, albumin, alanine ami-notransferase and aspartate aminotransferase were similar between animals in the control group at all time periods studied. Histological and histometric studies showed that the scaffold was coated with a cement-like substance, exhibiting many areas of mineralized structures. Very few osteocyte-like cells or lining-like cells were found inside the amorphous mineralized deposit. In vivo results allow us to consider this scaffold as a promising biomaterial to be applied in bone tissue engineering.Fil: Coletta, Dante Jesus. Universidad Nacional de Rosario. Facultad de Cs.medicas. Laboratorio de Biologia Osteoarticular, Ingenieria Tisular y Terapias Emergentes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnol.conicet - Rosario. Unidad de Direccion; ArgentinaFil: Missana, Liliana Raquel. Okayama University; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Martins, Talita. Universidade Federal de Minas Gerais; BrasilFil: Jammal, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: García, Luciano Andres. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Osteoarticular, Ingeniería Tisular y Terapias Emergentes; ArgentinaFil: Farez, Nayla. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Osteoarticular, Ingeniería Tisular y Terapias Emergentes; ArgentinaFil: Glee, Tomas De. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Osteoarticular, Ingeniería Tisular y Terapias Emergentes; ArgentinaFil: Paulo Mardegan Issa, João. Universidade de Sao Paulo; BrasilFil: Feldman, Sara. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Osteoarticular, Ingeniería Tisular y Terapias Emergentes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnol.conicet - Rosario. Unidad de Direccion; ArgentinaScientific Research Publisinhg2018-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/91947Coletta, Dante Jesus; Missana, Liliana Raquel; Martins, Talita; Jammal, María Victoria; García, Luciano Andres; et al.; Synthetic Three-Dimensional Scaffold for Application in the Regeneration of Bone Tissue; Scientific Research Publisinhg; Journal of Biomaterials and Nanobiotechnology; 9; 4; 9-2018; 277-2892158-70272158-7043CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.scirp.org/journal/paperinformation.aspx?paperid=87210info:eu-repo/semantics/altIdentifier/doi/10.4236/jbnb.2018.94016info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:12:42Zoai:ri.conicet.gov.ar:11336/91947instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:12:42.393CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Synthetic Three-Dimensional Scaffold for Application in the Regeneration of Bone Tissue
title Synthetic Three-Dimensional Scaffold for Application in the Regeneration of Bone Tissue
spellingShingle Synthetic Three-Dimensional Scaffold for Application in the Regeneration of Bone Tissue
Coletta, Dante Jesus
BIOMATERIAL SCAFFOLD
BONE
CHITOSAN
POLY(VINYL ALCOHOL-CO-VINYL ACETATE)
title_short Synthetic Three-Dimensional Scaffold for Application in the Regeneration of Bone Tissue
title_full Synthetic Three-Dimensional Scaffold for Application in the Regeneration of Bone Tissue
title_fullStr Synthetic Three-Dimensional Scaffold for Application in the Regeneration of Bone Tissue
title_full_unstemmed Synthetic Three-Dimensional Scaffold for Application in the Regeneration of Bone Tissue
title_sort Synthetic Three-Dimensional Scaffold for Application in the Regeneration of Bone Tissue
dc.creator.none.fl_str_mv Coletta, Dante Jesus
Missana, Liliana Raquel
Martins, Talita
Jammal, María Victoria
García, Luciano Andres
Farez, Nayla
Glee, Tomas De
Paulo Mardegan Issa, João
Feldman, Sara
author Coletta, Dante Jesus
author_facet Coletta, Dante Jesus
Missana, Liliana Raquel
Martins, Talita
Jammal, María Victoria
García, Luciano Andres
Farez, Nayla
Glee, Tomas De
Paulo Mardegan Issa, João
Feldman, Sara
author_role author
author2 Missana, Liliana Raquel
Martins, Talita
Jammal, María Victoria
García, Luciano Andres
Farez, Nayla
Glee, Tomas De
Paulo Mardegan Issa, João
Feldman, Sara
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BIOMATERIAL SCAFFOLD
BONE
CHITOSAN
POLY(VINYL ALCOHOL-CO-VINYL ACETATE)
topic BIOMATERIAL SCAFFOLD
BONE
CHITOSAN
POLY(VINYL ALCOHOL-CO-VINYL ACETATE)
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Bone tissue engineering aims to use biodegrade.able scaffolds to replace damaged tissue. This scaffold must be gradually degraded and replaced by tissue as similar as possible to the original one. In this work a hybrid porous scaffold containing chitosan, polyvinyl alcohol and bioactive glass was suc-cessfully obtained and subsequently characterized by scanning electron mi-croscopy. The scaffold presented satisfactory pore size range and open inter-connected pores, which are essential for tissue ingrowth. A cytotoxicity assay showed that this biomaterial allows adequate cell viability, so that it was con-sidered suitable for an in vivo experiment. Promising resuts were obtained with the implant of the scaffold in an experimental model of a New Zealand rabbit femur bone lesion. Clinical and biochemical parameters measured such as complete blood count, total serum proteins, albumin, alanine ami-notransferase and aspartate aminotransferase were similar between animals in the control group at all time periods studied. Histological and histometric studies showed that the scaffold was coated with a cement-like substance, exhibiting many areas of mineralized structures. Very few osteocyte-like cells or lining-like cells were found inside the amorphous mineralized deposit. In vivo results allow us to consider this scaffold as a promising biomaterial to be applied in bone tissue engineering.
Fil: Coletta, Dante Jesus. Universidad Nacional de Rosario. Facultad de Cs.medicas. Laboratorio de Biologia Osteoarticular, Ingenieria Tisular y Terapias Emergentes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnol.conicet - Rosario. Unidad de Direccion; Argentina
Fil: Missana, Liliana Raquel. Okayama University; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Martins, Talita. Universidade Federal de Minas Gerais; Brasil
Fil: Jammal, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: García, Luciano Andres. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Osteoarticular, Ingeniería Tisular y Terapias Emergentes; Argentina
Fil: Farez, Nayla. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Osteoarticular, Ingeniería Tisular y Terapias Emergentes; Argentina
Fil: Glee, Tomas De. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Osteoarticular, Ingeniería Tisular y Terapias Emergentes; Argentina
Fil: Paulo Mardegan Issa, João. Universidade de Sao Paulo; Brasil
Fil: Feldman, Sara. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Osteoarticular, Ingeniería Tisular y Terapias Emergentes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnol.conicet - Rosario. Unidad de Direccion; Argentina
description Bone tissue engineering aims to use biodegrade.able scaffolds to replace damaged tissue. This scaffold must be gradually degraded and replaced by tissue as similar as possible to the original one. In this work a hybrid porous scaffold containing chitosan, polyvinyl alcohol and bioactive glass was suc-cessfully obtained and subsequently characterized by scanning electron mi-croscopy. The scaffold presented satisfactory pore size range and open inter-connected pores, which are essential for tissue ingrowth. A cytotoxicity assay showed that this biomaterial allows adequate cell viability, so that it was con-sidered suitable for an in vivo experiment. Promising resuts were obtained with the implant of the scaffold in an experimental model of a New Zealand rabbit femur bone lesion. Clinical and biochemical parameters measured such as complete blood count, total serum proteins, albumin, alanine ami-notransferase and aspartate aminotransferase were similar between animals in the control group at all time periods studied. Histological and histometric studies showed that the scaffold was coated with a cement-like substance, exhibiting many areas of mineralized structures. Very few osteocyte-like cells or lining-like cells were found inside the amorphous mineralized deposit. In vivo results allow us to consider this scaffold as a promising biomaterial to be applied in bone tissue engineering.
publishDate 2018
dc.date.none.fl_str_mv 2018-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/91947
Coletta, Dante Jesus; Missana, Liliana Raquel; Martins, Talita; Jammal, María Victoria; García, Luciano Andres; et al.; Synthetic Three-Dimensional Scaffold for Application in the Regeneration of Bone Tissue; Scientific Research Publisinhg; Journal of Biomaterials and Nanobiotechnology; 9; 4; 9-2018; 277-289
2158-7027
2158-7043
CONICET Digital
CONICET
url http://hdl.handle.net/11336/91947
identifier_str_mv Coletta, Dante Jesus; Missana, Liliana Raquel; Martins, Talita; Jammal, María Victoria; García, Luciano Andres; et al.; Synthetic Three-Dimensional Scaffold for Application in the Regeneration of Bone Tissue; Scientific Research Publisinhg; Journal of Biomaterials and Nanobiotechnology; 9; 4; 9-2018; 277-289
2158-7027
2158-7043
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.scirp.org/journal/paperinformation.aspx?paperid=87210
info:eu-repo/semantics/altIdentifier/doi/10.4236/jbnb.2018.94016
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Scientific Research Publisinhg
publisher.none.fl_str_mv Scientific Research Publisinhg
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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