Lactobacillus crispatus BC1 biosurfactant delivered by hyalurosomes: an advanced strategy to counteract candida biofilm

Autores
Abruzzo, Angela; Giordani, Barbara; Parolin, Carola; de Gregorio, Priscilla Romina; Foschi, Claudio; Cerchiara, Teresa; Bigucci, Federica; Vitali, Beatrice; Luppi, Barbara
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The emergence of resistance to antifungal drugs has made the treatment of vulvovaginalcandidiasis (VVC) very challenging. Among natural substances, biosurfactants (BS) produced byLactobacillus have gained increasing interest in counteracting Candida infections for their provenanti-adhesive properties and safety profile. In the present study, liposomes (LP-BS) or liposomescoated with hyaluronic acid (HY-LP-BS) were prepared in the presence of the BS isolated from thevaginal strain Lactobacillus crispatus BC1 and characterized in terms of size, potential, stability and mucoadhesion. The anti-biofilm activity of free BS, LP-BS and HY-LP-BS was investigated againstdifferent Candida albicans and non-albicans strains (C. glabrata, C. lusitaniae, C. tropicalis, C. krusei and C. parapsilosis), clinically isolated from patients affected by VVC. The inhibition of biofilm formation and the dispersal of pre-formed biofilm were evaluated. The obtained phospholipid vesicles showed suitable size for vaginal application and good stability over the storage period. HY-LP-BS exhibited good mucoadhesive properties and the best anti-biofilm profile, both in preventing or limiting the surface colonization by a broad spectrum of Candida species. In conclusion, the formulation of a novel antifungal agent derived from the vaginal microbiota into mucoadhesive nanocarriers appears to be a promising biotherapeutic strategy to counteract vulvovaginal candidiasis.
Fil: Abruzzo, Angela. Universidad de Bologna; Italia
Fil: Giordani, Barbara. Universidad de Bologna; Italia
Fil: Parolin, Carola. Universidad de Bologna; Italia
Fil: de Gregorio, Priscilla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Foschi, Claudio. Universidad de Bologna; Italia
Fil: Cerchiara, Teresa. Universidad de Bologna; Italia
Fil: Bigucci, Federica. Universidad de Bologna; Italia
Fil: Vitali, Beatrice. Universidad de Bologna; Italia
Fil: Luppi, Barbara. Universidad de Bologna; Italia
Materia
BIOSURFACTANT
LACTOBACILLUS
LIPOSOMES
HYALUROSOMES
MUCOADHESION
CANDIDA
BIOFILM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/134600
Acceder
id CONICETDig_7d97212bab9b4cae44fabacd1342cd16
oai_identifier_str oai:ri.conicet.gov.ar:11336/134600
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lactobacillus crispatus BC1 biosurfactant delivered by hyalurosomes: an advanced strategy to counteract candida biofilmAbruzzo, AngelaGiordani, BarbaraParolin, Carolade Gregorio, Priscilla RominaFoschi, ClaudioCerchiara, TeresaBigucci, FedericaVitali, BeatriceLuppi, BarbaraBIOSURFACTANTLACTOBACILLUSLIPOSOMESHYALUROSOMESMUCOADHESIONCANDIDABIOFILMhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The emergence of resistance to antifungal drugs has made the treatment of vulvovaginalcandidiasis (VVC) very challenging. Among natural substances, biosurfactants (BS) produced byLactobacillus have gained increasing interest in counteracting Candida infections for their provenanti-adhesive properties and safety profile. In the present study, liposomes (LP-BS) or liposomescoated with hyaluronic acid (HY-LP-BS) were prepared in the presence of the BS isolated from thevaginal strain Lactobacillus crispatus BC1 and characterized in terms of size, potential, stability and mucoadhesion. The anti-biofilm activity of free BS, LP-BS and HY-LP-BS was investigated againstdifferent Candida albicans and non-albicans strains (C. glabrata, C. lusitaniae, C. tropicalis, C. krusei and C. parapsilosis), clinically isolated from patients affected by VVC. The inhibition of biofilm formation and the dispersal of pre-formed biofilm were evaluated. The obtained phospholipid vesicles showed suitable size for vaginal application and good stability over the storage period. HY-LP-BS exhibited good mucoadhesive properties and the best anti-biofilm profile, both in preventing or limiting the surface colonization by a broad spectrum of Candida species. In conclusion, the formulation of a novel antifungal agent derived from the vaginal microbiota into mucoadhesive nanocarriers appears to be a promising biotherapeutic strategy to counteract vulvovaginal candidiasis.Fil: Abruzzo, Angela. Universidad de Bologna; ItaliaFil: Giordani, Barbara. Universidad de Bologna; ItaliaFil: Parolin, Carola. Universidad de Bologna; ItaliaFil: de Gregorio, Priscilla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Foschi, Claudio. Universidad de Bologna; ItaliaFil: Cerchiara, Teresa. Universidad de Bologna; ItaliaFil: Bigucci, Federica. Universidad de Bologna; ItaliaFil: Vitali, Beatrice. Universidad de Bologna; ItaliaFil: Luppi, Barbara. Universidad de Bologna; ItaliaMultidisciplinary Digital Publishing Institute2021-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/134600Abruzzo, Angela; Giordani, Barbara; Parolin, Carola; de Gregorio, Priscilla Romina; Foschi, Claudio; et al.; Lactobacillus crispatus BC1 biosurfactant delivered by hyalurosomes: an advanced strategy to counteract candida biofilm; Multidisciplinary Digital Publishing Institute; Antibiotics; 10; 1; 1-2021; 1-142079-63822079-6382CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/antibiotics10010033info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2079-6382/10/1/33info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:12:36Zoai:ri.conicet.gov.ar:11336/134600instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:12:37.245CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lactobacillus crispatus BC1 biosurfactant delivered by hyalurosomes: an advanced strategy to counteract candida biofilm
title Lactobacillus crispatus BC1 biosurfactant delivered by hyalurosomes: an advanced strategy to counteract candida biofilm
spellingShingle Lactobacillus crispatus BC1 biosurfactant delivered by hyalurosomes: an advanced strategy to counteract candida biofilm
Abruzzo, Angela
BIOSURFACTANT
LACTOBACILLUS
LIPOSOMES
HYALUROSOMES
MUCOADHESION
CANDIDA
BIOFILM
title_short Lactobacillus crispatus BC1 biosurfactant delivered by hyalurosomes: an advanced strategy to counteract candida biofilm
title_full Lactobacillus crispatus BC1 biosurfactant delivered by hyalurosomes: an advanced strategy to counteract candida biofilm
title_fullStr Lactobacillus crispatus BC1 biosurfactant delivered by hyalurosomes: an advanced strategy to counteract candida biofilm
title_full_unstemmed Lactobacillus crispatus BC1 biosurfactant delivered by hyalurosomes: an advanced strategy to counteract candida biofilm
title_sort Lactobacillus crispatus BC1 biosurfactant delivered by hyalurosomes: an advanced strategy to counteract candida biofilm
dc.creator.none.fl_str_mv Abruzzo, Angela
Giordani, Barbara
Parolin, Carola
de Gregorio, Priscilla Romina
Foschi, Claudio
Cerchiara, Teresa
Bigucci, Federica
Vitali, Beatrice
Luppi, Barbara
author Abruzzo, Angela
author_facet Abruzzo, Angela
Giordani, Barbara
Parolin, Carola
de Gregorio, Priscilla Romina
Foschi, Claudio
Cerchiara, Teresa
Bigucci, Federica
Vitali, Beatrice
Luppi, Barbara
author_role author
author2 Giordani, Barbara
Parolin, Carola
de Gregorio, Priscilla Romina
Foschi, Claudio
Cerchiara, Teresa
Bigucci, Federica
Vitali, Beatrice
Luppi, Barbara
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BIOSURFACTANT
LACTOBACILLUS
LIPOSOMES
HYALUROSOMES
MUCOADHESION
CANDIDA
BIOFILM
topic BIOSURFACTANT
LACTOBACILLUS
LIPOSOMES
HYALUROSOMES
MUCOADHESION
CANDIDA
BIOFILM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The emergence of resistance to antifungal drugs has made the treatment of vulvovaginalcandidiasis (VVC) very challenging. Among natural substances, biosurfactants (BS) produced byLactobacillus have gained increasing interest in counteracting Candida infections for their provenanti-adhesive properties and safety profile. In the present study, liposomes (LP-BS) or liposomescoated with hyaluronic acid (HY-LP-BS) were prepared in the presence of the BS isolated from thevaginal strain Lactobacillus crispatus BC1 and characterized in terms of size, potential, stability and mucoadhesion. The anti-biofilm activity of free BS, LP-BS and HY-LP-BS was investigated againstdifferent Candida albicans and non-albicans strains (C. glabrata, C. lusitaniae, C. tropicalis, C. krusei and C. parapsilosis), clinically isolated from patients affected by VVC. The inhibition of biofilm formation and the dispersal of pre-formed biofilm were evaluated. The obtained phospholipid vesicles showed suitable size for vaginal application and good stability over the storage period. HY-LP-BS exhibited good mucoadhesive properties and the best anti-biofilm profile, both in preventing or limiting the surface colonization by a broad spectrum of Candida species. In conclusion, the formulation of a novel antifungal agent derived from the vaginal microbiota into mucoadhesive nanocarriers appears to be a promising biotherapeutic strategy to counteract vulvovaginal candidiasis.
Fil: Abruzzo, Angela. Universidad de Bologna; Italia
Fil: Giordani, Barbara. Universidad de Bologna; Italia
Fil: Parolin, Carola. Universidad de Bologna; Italia
Fil: de Gregorio, Priscilla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Foschi, Claudio. Universidad de Bologna; Italia
Fil: Cerchiara, Teresa. Universidad de Bologna; Italia
Fil: Bigucci, Federica. Universidad de Bologna; Italia
Fil: Vitali, Beatrice. Universidad de Bologna; Italia
Fil: Luppi, Barbara. Universidad de Bologna; Italia
description The emergence of resistance to antifungal drugs has made the treatment of vulvovaginalcandidiasis (VVC) very challenging. Among natural substances, biosurfactants (BS) produced byLactobacillus have gained increasing interest in counteracting Candida infections for their provenanti-adhesive properties and safety profile. In the present study, liposomes (LP-BS) or liposomescoated with hyaluronic acid (HY-LP-BS) were prepared in the presence of the BS isolated from thevaginal strain Lactobacillus crispatus BC1 and characterized in terms of size, potential, stability and mucoadhesion. The anti-biofilm activity of free BS, LP-BS and HY-LP-BS was investigated againstdifferent Candida albicans and non-albicans strains (C. glabrata, C. lusitaniae, C. tropicalis, C. krusei and C. parapsilosis), clinically isolated from patients affected by VVC. The inhibition of biofilm formation and the dispersal of pre-formed biofilm were evaluated. The obtained phospholipid vesicles showed suitable size for vaginal application and good stability over the storage period. HY-LP-BS exhibited good mucoadhesive properties and the best anti-biofilm profile, both in preventing or limiting the surface colonization by a broad spectrum of Candida species. In conclusion, the formulation of a novel antifungal agent derived from the vaginal microbiota into mucoadhesive nanocarriers appears to be a promising biotherapeutic strategy to counteract vulvovaginal candidiasis.
publishDate 2021
dc.date.none.fl_str_mv 2021-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/134600
Abruzzo, Angela; Giordani, Barbara; Parolin, Carola; de Gregorio, Priscilla Romina; Foschi, Claudio; et al.; Lactobacillus crispatus BC1 biosurfactant delivered by hyalurosomes: an advanced strategy to counteract candida biofilm; Multidisciplinary Digital Publishing Institute; Antibiotics; 10; 1; 1-2021; 1-14
2079-6382
2079-6382
CONICET Digital
CONICET
url http://hdl.handle.net/11336/134600
identifier_str_mv Abruzzo, Angela; Giordani, Barbara; Parolin, Carola; de Gregorio, Priscilla Romina; Foschi, Claudio; et al.; Lactobacillus crispatus BC1 biosurfactant delivered by hyalurosomes: an advanced strategy to counteract candida biofilm; Multidisciplinary Digital Publishing Institute; Antibiotics; 10; 1; 1-2021; 1-14
2079-6382
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/antibiotics10010033
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2079-6382/10/1/33
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980658992906240
score 12.993085

Publicaciones similares