Biosurfactant from vaginal Lactobacillus crispatus BC1 as a promising agent to interfere with Candida adhesion

Autores
de Gregorio, Priscilla Romina; Parolin, Carola; Abruzzo, Angela; Luppi, Barbara; Protti, Michele; Mercolini, Laura; Silva, Jessica Alejandra; Giordani, Barbara; Marangoni, Antonella; Nader, Maria Elena Fatima; Vitali, Beatrice
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lactobacillus spp. dominating the vaginal microbiota of healthy women contribute to the prevention of urogenital and sexually transmitted infections. Their protective role in the vagina can be mediated by Lactobacillus cells themselves, metabolites or bacterial components, able to interfere with pathogen adhesion and infectivity. Vulvovaginal candidiasis (VVC) is a common genital infection, caused by the overgrowth of opportunistic Candida spp. including C. albicans, C. glabrata, C. krusei and C. tropicalis. Azole antifungal drugs are not always efcient in resolv ing VVC and preventing recurrent infections, thus alternative anti-Candida agents based on vaginal probiotics have gained more importance. The present work aims to chemically characterize the biosurfactant (BS) isolated from a vaginal Lactobacillus crispatus strain, L. crispatus BC1, and to investigate its safety and antiadhesive/antimicrobial activ ity against Candida spp., employing in vitro and in vivo assays. Results: BS isolated from vaginal L. crispatus BC1 was characterised as non-homogeneous lipopeptide molecules with a critical micellar concentration value of 2 mg/mL, and good emulsifcation and mucoadhesive properties. At 1.25 mg/mL, the BS was not cytotoxic and reduced Candida strains? ability to adhere to human cervical epithelial cells, mainly by exclusion mechanism. Moreover, intravaginal (i.va.) inoculation of BS in a murine experimental model was safe and did not perturb vaginal cytology, histology and cultivable vaginal microbiota. In the case of i.va. challenge of mice with C. albicans, BS was able to reduce leukocyte infux. Conclusions: These results indicate that BS from vaginal L. crispatus BC1 is able to interfere with Candida adhesion in vitro and in vivo, and suggest its potential as a preventive agent to reduce mucosal damage occasioned by Candida during VVC.
Fil: de Gregorio, Priscilla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Parolin, Carola. Universidad de Bologna; Italia
Fil: Abruzzo, Angela. Universidad de Bologna; Italia
Fil: Luppi, Barbara. Universidad de Bologna; Italia
Fil: Protti, Michele. Universidad de Bologna; Italia
Fil: Mercolini, Laura. Universidad de Bologna; Italia
Fil: Silva, Jessica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Giordani, Barbara. Universidad de Bologna; Italia
Fil: Marangoni, Antonella. Universidad de Bologna; Italia
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Vitali, Beatrice. Universidad de Bologna; Italia
Materia
VAGINA
LACTOBACILLUS CRISPATUS
CÁNDIDA SPP.
BIOSURFACTANT
MURINE MODEL
HELA CELLS
VULVOVAGINAL CANDIDIASIS
ANTY-CANDIDA ACTIVITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/138222
Acceder
id CONICETDig_bc8604a6f23eed2344ab05f250fe3a0e
oai_identifier_str oai:ri.conicet.gov.ar:11336/138222
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biosurfactant from vaginal Lactobacillus crispatus BC1 as a promising agent to interfere with Candida adhesionde Gregorio, Priscilla RominaParolin, CarolaAbruzzo, AngelaLuppi, BarbaraProtti, MicheleMercolini, LauraSilva, Jessica AlejandraGiordani, BarbaraMarangoni, AntonellaNader, Maria Elena FatimaVitali, BeatriceVAGINALACTOBACILLUS CRISPATUSCÁNDIDA SPP.BIOSURFACTANTMURINE MODELHELA CELLSVULVOVAGINAL CANDIDIASISANTY-CANDIDA ACTIVITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Lactobacillus spp. dominating the vaginal microbiota of healthy women contribute to the prevention of urogenital and sexually transmitted infections. Their protective role in the vagina can be mediated by Lactobacillus cells themselves, metabolites or bacterial components, able to interfere with pathogen adhesion and infectivity. Vulvovaginal candidiasis (VVC) is a common genital infection, caused by the overgrowth of opportunistic Candida spp. including C. albicans, C. glabrata, C. krusei and C. tropicalis. Azole antifungal drugs are not always efcient in resolv ing VVC and preventing recurrent infections, thus alternative anti-Candida agents based on vaginal probiotics have gained more importance. The present work aims to chemically characterize the biosurfactant (BS) isolated from a vaginal Lactobacillus crispatus strain, L. crispatus BC1, and to investigate its safety and antiadhesive/antimicrobial activ ity against Candida spp., employing in vitro and in vivo assays. Results: BS isolated from vaginal L. crispatus BC1 was characterised as non-homogeneous lipopeptide molecules with a critical micellar concentration value of 2 mg/mL, and good emulsifcation and mucoadhesive properties. At 1.25 mg/mL, the BS was not cytotoxic and reduced Candida strains? ability to adhere to human cervical epithelial cells, mainly by exclusion mechanism. Moreover, intravaginal (i.va.) inoculation of BS in a murine experimental model was safe and did not perturb vaginal cytology, histology and cultivable vaginal microbiota. In the case of i.va. challenge of mice with C. albicans, BS was able to reduce leukocyte infux. Conclusions: These results indicate that BS from vaginal L. crispatus BC1 is able to interfere with Candida adhesion in vitro and in vivo, and suggest its potential as a preventive agent to reduce mucosal damage occasioned by Candida during VVC.Fil: de Gregorio, Priscilla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Parolin, Carola. Universidad de Bologna; ItaliaFil: Abruzzo, Angela. Universidad de Bologna; ItaliaFil: Luppi, Barbara. Universidad de Bologna; ItaliaFil: Protti, Michele. Universidad de Bologna; ItaliaFil: Mercolini, Laura. Universidad de Bologna; ItaliaFil: Silva, Jessica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Giordani, Barbara. Universidad de Bologna; ItaliaFil: Marangoni, Antonella. Universidad de Bologna; ItaliaFil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Vitali, Beatrice. Universidad de Bologna; ItaliaBioMed Central2020-06-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/138222de Gregorio, Priscilla Romina; Parolin, Carola; Abruzzo, Angela; Luppi, Barbara; Protti, Michele; et al.; Biosurfactant from vaginal Lactobacillus crispatus BC1 as a promising agent to interfere with Candida adhesion; BioMed Central; Microbial Cell Factories; 19; 133; 18-6-2020; 1-161475-2859CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1186/s12934-020-01390-5info:eu-repo/semantics/altIdentifier/url/https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-020-01390-5info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:11:38Zoai:ri.conicet.gov.ar:11336/138222instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:11:38.617CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biosurfactant from vaginal Lactobacillus crispatus BC1 as a promising agent to interfere with Candida adhesion
title Biosurfactant from vaginal Lactobacillus crispatus BC1 as a promising agent to interfere with Candida adhesion
spellingShingle Biosurfactant from vaginal Lactobacillus crispatus BC1 as a promising agent to interfere with Candida adhesion
de Gregorio, Priscilla Romina
VAGINA
LACTOBACILLUS CRISPATUS
CÁNDIDA SPP.
BIOSURFACTANT
MURINE MODEL
HELA CELLS
VULVOVAGINAL CANDIDIASIS
ANTY-CANDIDA ACTIVITY
title_short Biosurfactant from vaginal Lactobacillus crispatus BC1 as a promising agent to interfere with Candida adhesion
title_full Biosurfactant from vaginal Lactobacillus crispatus BC1 as a promising agent to interfere with Candida adhesion
title_fullStr Biosurfactant from vaginal Lactobacillus crispatus BC1 as a promising agent to interfere with Candida adhesion
title_full_unstemmed Biosurfactant from vaginal Lactobacillus crispatus BC1 as a promising agent to interfere with Candida adhesion
title_sort Biosurfactant from vaginal Lactobacillus crispatus BC1 as a promising agent to interfere with Candida adhesion
dc.creator.none.fl_str_mv de Gregorio, Priscilla Romina
Parolin, Carola
Abruzzo, Angela
Luppi, Barbara
Protti, Michele
Mercolini, Laura
Silva, Jessica Alejandra
Giordani, Barbara
Marangoni, Antonella
Nader, Maria Elena Fatima
Vitali, Beatrice
author de Gregorio, Priscilla Romina
author_facet de Gregorio, Priscilla Romina
Parolin, Carola
Abruzzo, Angela
Luppi, Barbara
Protti, Michele
Mercolini, Laura
Silva, Jessica Alejandra
Giordani, Barbara
Marangoni, Antonella
Nader, Maria Elena Fatima
Vitali, Beatrice
author_role author
author2 Parolin, Carola
Abruzzo, Angela
Luppi, Barbara
Protti, Michele
Mercolini, Laura
Silva, Jessica Alejandra
Giordani, Barbara
Marangoni, Antonella
Nader, Maria Elena Fatima
Vitali, Beatrice
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv VAGINA
LACTOBACILLUS CRISPATUS
CÁNDIDA SPP.
BIOSURFACTANT
MURINE MODEL
HELA CELLS
VULVOVAGINAL CANDIDIASIS
ANTY-CANDIDA ACTIVITY
topic VAGINA
LACTOBACILLUS CRISPATUS
CÁNDIDA SPP.
BIOSURFACTANT
MURINE MODEL
HELA CELLS
VULVOVAGINAL CANDIDIASIS
ANTY-CANDIDA ACTIVITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Lactobacillus spp. dominating the vaginal microbiota of healthy women contribute to the prevention of urogenital and sexually transmitted infections. Their protective role in the vagina can be mediated by Lactobacillus cells themselves, metabolites or bacterial components, able to interfere with pathogen adhesion and infectivity. Vulvovaginal candidiasis (VVC) is a common genital infection, caused by the overgrowth of opportunistic Candida spp. including C. albicans, C. glabrata, C. krusei and C. tropicalis. Azole antifungal drugs are not always efcient in resolv ing VVC and preventing recurrent infections, thus alternative anti-Candida agents based on vaginal probiotics have gained more importance. The present work aims to chemically characterize the biosurfactant (BS) isolated from a vaginal Lactobacillus crispatus strain, L. crispatus BC1, and to investigate its safety and antiadhesive/antimicrobial activ ity against Candida spp., employing in vitro and in vivo assays. Results: BS isolated from vaginal L. crispatus BC1 was characterised as non-homogeneous lipopeptide molecules with a critical micellar concentration value of 2 mg/mL, and good emulsifcation and mucoadhesive properties. At 1.25 mg/mL, the BS was not cytotoxic and reduced Candida strains? ability to adhere to human cervical epithelial cells, mainly by exclusion mechanism. Moreover, intravaginal (i.va.) inoculation of BS in a murine experimental model was safe and did not perturb vaginal cytology, histology and cultivable vaginal microbiota. In the case of i.va. challenge of mice with C. albicans, BS was able to reduce leukocyte infux. Conclusions: These results indicate that BS from vaginal L. crispatus BC1 is able to interfere with Candida adhesion in vitro and in vivo, and suggest its potential as a preventive agent to reduce mucosal damage occasioned by Candida during VVC.
Fil: de Gregorio, Priscilla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Parolin, Carola. Universidad de Bologna; Italia
Fil: Abruzzo, Angela. Universidad de Bologna; Italia
Fil: Luppi, Barbara. Universidad de Bologna; Italia
Fil: Protti, Michele. Universidad de Bologna; Italia
Fil: Mercolini, Laura. Universidad de Bologna; Italia
Fil: Silva, Jessica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Giordani, Barbara. Universidad de Bologna; Italia
Fil: Marangoni, Antonella. Universidad de Bologna; Italia
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Vitali, Beatrice. Universidad de Bologna; Italia
description Lactobacillus spp. dominating the vaginal microbiota of healthy women contribute to the prevention of urogenital and sexually transmitted infections. Their protective role in the vagina can be mediated by Lactobacillus cells themselves, metabolites or bacterial components, able to interfere with pathogen adhesion and infectivity. Vulvovaginal candidiasis (VVC) is a common genital infection, caused by the overgrowth of opportunistic Candida spp. including C. albicans, C. glabrata, C. krusei and C. tropicalis. Azole antifungal drugs are not always efcient in resolv ing VVC and preventing recurrent infections, thus alternative anti-Candida agents based on vaginal probiotics have gained more importance. The present work aims to chemically characterize the biosurfactant (BS) isolated from a vaginal Lactobacillus crispatus strain, L. crispatus BC1, and to investigate its safety and antiadhesive/antimicrobial activ ity against Candida spp., employing in vitro and in vivo assays. Results: BS isolated from vaginal L. crispatus BC1 was characterised as non-homogeneous lipopeptide molecules with a critical micellar concentration value of 2 mg/mL, and good emulsifcation and mucoadhesive properties. At 1.25 mg/mL, the BS was not cytotoxic and reduced Candida strains? ability to adhere to human cervical epithelial cells, mainly by exclusion mechanism. Moreover, intravaginal (i.va.) inoculation of BS in a murine experimental model was safe and did not perturb vaginal cytology, histology and cultivable vaginal microbiota. In the case of i.va. challenge of mice with C. albicans, BS was able to reduce leukocyte infux. Conclusions: These results indicate that BS from vaginal L. crispatus BC1 is able to interfere with Candida adhesion in vitro and in vivo, and suggest its potential as a preventive agent to reduce mucosal damage occasioned by Candida during VVC.
publishDate 2020
dc.date.none.fl_str_mv 2020-06-18
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/138222
de Gregorio, Priscilla Romina; Parolin, Carola; Abruzzo, Angela; Luppi, Barbara; Protti, Michele; et al.; Biosurfactant from vaginal Lactobacillus crispatus BC1 as a promising agent to interfere with Candida adhesion; BioMed Central; Microbial Cell Factories; 19; 133; 18-6-2020; 1-16
1475-2859
CONICET Digital
CONICET
url http://hdl.handle.net/11336/138222
identifier_str_mv de Gregorio, Priscilla Romina; Parolin, Carola; Abruzzo, Angela; Luppi, Barbara; Protti, Michele; et al.; Biosurfactant from vaginal Lactobacillus crispatus BC1 as a promising agent to interfere with Candida adhesion; BioMed Central; Microbial Cell Factories; 19; 133; 18-6-2020; 1-16
1475-2859
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/s12934-020-01390-5
info:eu-repo/semantics/altIdentifier/url/https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-020-01390-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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