upramolecular complex based on MWNTs/Boltorn H40 provides fast response to a Sandwich-type amperometric lactate biosensor

Autores
Romero, Marcelo Ricardo; Peralta, Damian Oscar; Alvarez Igarzabal, Cecilia Ines; Baruzzi, Ana Maria; Strumia, Miriam Cristina; Garay, Fernando Sebastian
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Most sandwich-type biosensors can be used several times, since their long-time stability generally ranges from weeks to several months. However, the response-time of this kind of sensors is typically slow because reagents and products have to diffuse through filtering membranes and the enzymatic matrix. In this manuscript, multiwalled carbon nanotubes (MWNTs) have been used for wiring the enzymatic matrix to the electrode surface. To achieve this, the hyperbranched polymer Boltorn H40 (BH40) has been self-assembled at the surface of MWNTs. After the assembling, the suspension of BH40-MWNTs became stable and its functionality was tested by replacing mucin in the enzymatic matrix of a previously reported sandwich-type lactate biosensor. The inclusion of BH40-MWNTs in the biosensor: increased the diffusion coefficient of soluble species by decreasing the elastic properties of the enzymatic matrix, kept the characteristics of the microenvironment where the enzyme is stored, and wired the enzymatic matrix through a 13 μm thick polycarbonate membrane to the electrode surface. It is important to understand that the superstructure of BH40-MWNTs does not increase the active surface of the electrode. Instead of this, it is a conductive 3D network that connects the enzymatic matrix to the electrode. As a result, the response-time of this novel sandwich-type lactate biosensor is much shorter than that of conventional biosensors because the mediator of the enzyme (oxygen) can be regenerated at the enzymatic matrix and it does not have to diffuse to the electrode surface.
Fil: Romero, Marcelo Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Peralta, Damian Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Alvarez Igarzabal, Cecilia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Baruzzi, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Strumia, Miriam Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Garay, Fernando Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia
SELF-ASSAMBLED
SANDWICH-TYPE BIOSENSOR
LACTATE
NANOTUBE
HYDROGEL
HYPERBRANCHED
Nivel de accesibilidad
acceso embargado
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/20115
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling upramolecular complex based on MWNTs/Boltorn H40 provides fast response to a Sandwich-type amperometric lactate biosensorRomero, Marcelo RicardoPeralta, Damian OscarAlvarez Igarzabal, Cecilia InesBaruzzi, Ana MariaStrumia, Miriam CristinaGaray, Fernando SebastianSELF-ASSAMBLEDSANDWICH-TYPE BIOSENSORLACTATENANOTUBEHYDROGELHYPERBRANCHEDhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Most sandwich-type biosensors can be used several times, since their long-time stability generally ranges from weeks to several months. However, the response-time of this kind of sensors is typically slow because reagents and products have to diffuse through filtering membranes and the enzymatic matrix. In this manuscript, multiwalled carbon nanotubes (MWNTs) have been used for wiring the enzymatic matrix to the electrode surface. To achieve this, the hyperbranched polymer Boltorn H40 (BH40) has been self-assembled at the surface of MWNTs. After the assembling, the suspension of BH40-MWNTs became stable and its functionality was tested by replacing mucin in the enzymatic matrix of a previously reported sandwich-type lactate biosensor. The inclusion of BH40-MWNTs in the biosensor: increased the diffusion coefficient of soluble species by decreasing the elastic properties of the enzymatic matrix, kept the characteristics of the microenvironment where the enzyme is stored, and wired the enzymatic matrix through a 13 μm thick polycarbonate membrane to the electrode surface. It is important to understand that the superstructure of BH40-MWNTs does not increase the active surface of the electrode. Instead of this, it is a conductive 3D network that connects the enzymatic matrix to the electrode. As a result, the response-time of this novel sandwich-type lactate biosensor is much shorter than that of conventional biosensors because the mediator of the enzyme (oxygen) can be regenerated at the enzymatic matrix and it does not have to diffuse to the electrode surface.Fil: Romero, Marcelo Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Peralta, Damian Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Alvarez Igarzabal, Cecilia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Baruzzi, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Strumia, Miriam Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Garay, Fernando Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaElsevier Science Sa2017-01-05info:eu-repo/date/embargoEnd/2019-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/20115Romero, Marcelo Ricardo; Peralta, Damian Oscar; Alvarez Igarzabal, Cecilia Ines; Baruzzi, Ana Maria; Strumia, Miriam Cristina; et al.; upramolecular complex based on MWNTs/Boltorn H40 provides fast response to a Sandwich-type amperometric lactate biosensor; Elsevier Science Sa; Sensors and Actuators B: Chemical; 244; 05-1-2017; 577-5840925-4005CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2017.01.011info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925400517300114info:eu-repo/semantics/embargoedAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:41:25Zoai:ri.conicet.gov.ar:11336/20115instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:41:25.556CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv upramolecular complex based on MWNTs/Boltorn H40 provides fast response to a Sandwich-type amperometric lactate biosensor
title upramolecular complex based on MWNTs/Boltorn H40 provides fast response to a Sandwich-type amperometric lactate biosensor
spellingShingle upramolecular complex based on MWNTs/Boltorn H40 provides fast response to a Sandwich-type amperometric lactate biosensor
Romero, Marcelo Ricardo
SELF-ASSAMBLED
SANDWICH-TYPE BIOSENSOR
LACTATE
NANOTUBE
HYDROGEL
HYPERBRANCHED
title_short upramolecular complex based on MWNTs/Boltorn H40 provides fast response to a Sandwich-type amperometric lactate biosensor
title_full upramolecular complex based on MWNTs/Boltorn H40 provides fast response to a Sandwich-type amperometric lactate biosensor
title_fullStr upramolecular complex based on MWNTs/Boltorn H40 provides fast response to a Sandwich-type amperometric lactate biosensor
title_full_unstemmed upramolecular complex based on MWNTs/Boltorn H40 provides fast response to a Sandwich-type amperometric lactate biosensor
title_sort upramolecular complex based on MWNTs/Boltorn H40 provides fast response to a Sandwich-type amperometric lactate biosensor
dc.creator.none.fl_str_mv Romero, Marcelo Ricardo
Peralta, Damian Oscar
Alvarez Igarzabal, Cecilia Ines
Baruzzi, Ana Maria
Strumia, Miriam Cristina
Garay, Fernando Sebastian
author Romero, Marcelo Ricardo
author_facet Romero, Marcelo Ricardo
Peralta, Damian Oscar
Alvarez Igarzabal, Cecilia Ines
Baruzzi, Ana Maria
Strumia, Miriam Cristina
Garay, Fernando Sebastian
author_role author
author2 Peralta, Damian Oscar
Alvarez Igarzabal, Cecilia Ines
Baruzzi, Ana Maria
Strumia, Miriam Cristina
Garay, Fernando Sebastian
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv SELF-ASSAMBLED
SANDWICH-TYPE BIOSENSOR
LACTATE
NANOTUBE
HYDROGEL
HYPERBRANCHED
topic SELF-ASSAMBLED
SANDWICH-TYPE BIOSENSOR
LACTATE
NANOTUBE
HYDROGEL
HYPERBRANCHED
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Most sandwich-type biosensors can be used several times, since their long-time stability generally ranges from weeks to several months. However, the response-time of this kind of sensors is typically slow because reagents and products have to diffuse through filtering membranes and the enzymatic matrix. In this manuscript, multiwalled carbon nanotubes (MWNTs) have been used for wiring the enzymatic matrix to the electrode surface. To achieve this, the hyperbranched polymer Boltorn H40 (BH40) has been self-assembled at the surface of MWNTs. After the assembling, the suspension of BH40-MWNTs became stable and its functionality was tested by replacing mucin in the enzymatic matrix of a previously reported sandwich-type lactate biosensor. The inclusion of BH40-MWNTs in the biosensor: increased the diffusion coefficient of soluble species by decreasing the elastic properties of the enzymatic matrix, kept the characteristics of the microenvironment where the enzyme is stored, and wired the enzymatic matrix through a 13 μm thick polycarbonate membrane to the electrode surface. It is important to understand that the superstructure of BH40-MWNTs does not increase the active surface of the electrode. Instead of this, it is a conductive 3D network that connects the enzymatic matrix to the electrode. As a result, the response-time of this novel sandwich-type lactate biosensor is much shorter than that of conventional biosensors because the mediator of the enzyme (oxygen) can be regenerated at the enzymatic matrix and it does not have to diffuse to the electrode surface.
Fil: Romero, Marcelo Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Peralta, Damian Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Alvarez Igarzabal, Cecilia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Baruzzi, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Strumia, Miriam Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Garay, Fernando Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description Most sandwich-type biosensors can be used several times, since their long-time stability generally ranges from weeks to several months. However, the response-time of this kind of sensors is typically slow because reagents and products have to diffuse through filtering membranes and the enzymatic matrix. In this manuscript, multiwalled carbon nanotubes (MWNTs) have been used for wiring the enzymatic matrix to the electrode surface. To achieve this, the hyperbranched polymer Boltorn H40 (BH40) has been self-assembled at the surface of MWNTs. After the assembling, the suspension of BH40-MWNTs became stable and its functionality was tested by replacing mucin in the enzymatic matrix of a previously reported sandwich-type lactate biosensor. The inclusion of BH40-MWNTs in the biosensor: increased the diffusion coefficient of soluble species by decreasing the elastic properties of the enzymatic matrix, kept the characteristics of the microenvironment where the enzyme is stored, and wired the enzymatic matrix through a 13 μm thick polycarbonate membrane to the electrode surface. It is important to understand that the superstructure of BH40-MWNTs does not increase the active surface of the electrode. Instead of this, it is a conductive 3D network that connects the enzymatic matrix to the electrode. As a result, the response-time of this novel sandwich-type lactate biosensor is much shorter than that of conventional biosensors because the mediator of the enzyme (oxygen) can be regenerated at the enzymatic matrix and it does not have to diffuse to the electrode surface.
publishDate 2017
dc.date.none.fl_str_mv 2017-01-05
info:eu-repo/date/embargoEnd/2019-02-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/20115
Romero, Marcelo Ricardo; Peralta, Damian Oscar; Alvarez Igarzabal, Cecilia Ines; Baruzzi, Ana Maria; Strumia, Miriam Cristina; et al.; upramolecular complex based on MWNTs/Boltorn H40 provides fast response to a Sandwich-type amperometric lactate biosensor; Elsevier Science Sa; Sensors and Actuators B: Chemical; 244; 05-1-2017; 577-584
0925-4005
CONICET Digital
CONICET
url http://hdl.handle.net/11336/20115
identifier_str_mv Romero, Marcelo Ricardo; Peralta, Damian Oscar; Alvarez Igarzabal, Cecilia Ines; Baruzzi, Ana Maria; Strumia, Miriam Cristina; et al.; upramolecular complex based on MWNTs/Boltorn H40 provides fast response to a Sandwich-type amperometric lactate biosensor; Elsevier Science Sa; Sensors and Actuators B: Chemical; 244; 05-1-2017; 577-584
0925-4005
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2017.01.011
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925400517300114
dc.rights.none.fl_str_mv info:eu-repo/semantics/embargoedAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv embargoedAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Sa
publisher.none.fl_str_mv Elsevier Science Sa
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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