Tunable temperature responsive liquid chromatography through thiolactone-based immobilization of poly(N-isopropylacrylamide)
- Autores
- Satti, Angel Jose; Espeel, Pieter; Martens, Steven; Van Hoeylandt, Tim; Du Prez, Filip E.; Lynen, Frederic
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- A straightforward and efficient functionalization of aminopropylsilica with polymeric structures is described for the development of temperature responsive stationary phases applicable in purely aqueous liquid chromatography. The immobilization of the thermoresponsive polymers involves a thiolactone-based ring opening using the primary amines in aminopropylsilica, with a simultaneous one-pot, thiol-ene functionalization with an acrylate of choice. This mild, straightforward and modular grafting process results in high polymer coupling yields. By variation of the acrylate for the thiol-ene reaction, different stationary phases can be readily obtained. Two stationary phases as a result of the modular modification of aminopropylsilica were evaluated with test mixtures of hydrophobic analytes and a mixture of di- and tripeptides. Analyses using the 5 μm material packed in 10 cm x 4.6 mm columns revealed high hydrophobic retention, which proved adaptable as a function of the temperature in aqueous mobile phases. High versus low retention were obtained at temperatures above and below the lower critical solution temperature of the polymer, respectively. Moreover, the columns depict potential for diastereoisomeric peptide separation. Finally, the lower retention, observed when using PEGylated silica, illustrates the potential of the approach for modular stationary phase tuning.
Fil: Satti, Angel Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (i); Argentina. University Of Ghent; Bélgica
Fil: Espeel, Pieter. University Of Ghent; Bélgica
Fil: Martens, Steven. University Of Ghent; Bélgica
Fil: Van Hoeylandt, Tim. University Of Ghent; Bélgica
Fil: Du Prez, Filip E.. University Of Ghent; Bélgica
Fil: Lynen, Frederic. University Of Ghent; Bélgica - Materia
-
Hplc
Thiol-Ene Conjugation
Thiolactone
Poly(N-Isopropylacrylamide)
Purely Aqueous - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/4654
Ver los metadatos del registro completo
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Tunable temperature responsive liquid chromatography through thiolactone-based immobilization of poly(N-isopropylacrylamide)Satti, Angel JoseEspeel, PieterMartens, StevenVan Hoeylandt, TimDu Prez, Filip E.Lynen, FredericHplcThiol-Ene ConjugationThiolactonePoly(N-Isopropylacrylamide)Purely Aqueoushttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A straightforward and efficient functionalization of aminopropylsilica with polymeric structures is described for the development of temperature responsive stationary phases applicable in purely aqueous liquid chromatography. The immobilization of the thermoresponsive polymers involves a thiolactone-based ring opening using the primary amines in aminopropylsilica, with a simultaneous one-pot, thiol-ene functionalization with an acrylate of choice. This mild, straightforward and modular grafting process results in high polymer coupling yields. By variation of the acrylate for the thiol-ene reaction, different stationary phases can be readily obtained. Two stationary phases as a result of the modular modification of aminopropylsilica were evaluated with test mixtures of hydrophobic analytes and a mixture of di- and tripeptides. Analyses using the 5 μm material packed in 10 cm x 4.6 mm columns revealed high hydrophobic retention, which proved adaptable as a function of the temperature in aqueous mobile phases. High versus low retention were obtained at temperatures above and below the lower critical solution temperature of the polymer, respectively. Moreover, the columns depict potential for diastereoisomeric peptide separation. Finally, the lower retention, observed when using PEGylated silica, illustrates the potential of the approach for modular stationary phase tuning.Fil: Satti, Angel Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (i); Argentina. University Of Ghent; BélgicaFil: Espeel, Pieter. University Of Ghent; BélgicaFil: Martens, Steven. University Of Ghent; BélgicaFil: Van Hoeylandt, Tim. University Of Ghent; BélgicaFil: Du Prez, Filip E.. University Of Ghent; BélgicaFil: Lynen, Frederic. University Of Ghent; BélgicaElsevier Science2015-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4654Satti, Angel Jose; Espeel, Pieter; Martens, Steven; Van Hoeylandt, Tim; Du Prez, Filip E.; et al.; Tunable temperature responsive liquid chromatography through thiolactone-based immobilization of poly(N-isopropylacrylamide); Elsevier Science; Journal Of Chromatography - A; 1426; 11-2015; 126-1320021-9673enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S002196731501691Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.chroma.2015.11.063info:eu-repo/semantics/altIdentifier/issn/0021-9673info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:44:36Zoai:ri.conicet.gov.ar:11336/4654instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:44:36.556CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Tunable temperature responsive liquid chromatography through thiolactone-based immobilization of poly(N-isopropylacrylamide) |
title |
Tunable temperature responsive liquid chromatography through thiolactone-based immobilization of poly(N-isopropylacrylamide) |
spellingShingle |
Tunable temperature responsive liquid chromatography through thiolactone-based immobilization of poly(N-isopropylacrylamide) Satti, Angel Jose Hplc Thiol-Ene Conjugation Thiolactone Poly(N-Isopropylacrylamide) Purely Aqueous |
title_short |
Tunable temperature responsive liquid chromatography through thiolactone-based immobilization of poly(N-isopropylacrylamide) |
title_full |
Tunable temperature responsive liquid chromatography through thiolactone-based immobilization of poly(N-isopropylacrylamide) |
title_fullStr |
Tunable temperature responsive liquid chromatography through thiolactone-based immobilization of poly(N-isopropylacrylamide) |
title_full_unstemmed |
Tunable temperature responsive liquid chromatography through thiolactone-based immobilization of poly(N-isopropylacrylamide) |
title_sort |
Tunable temperature responsive liquid chromatography through thiolactone-based immobilization of poly(N-isopropylacrylamide) |
dc.creator.none.fl_str_mv |
Satti, Angel Jose Espeel, Pieter Martens, Steven Van Hoeylandt, Tim Du Prez, Filip E. Lynen, Frederic |
author |
Satti, Angel Jose |
author_facet |
Satti, Angel Jose Espeel, Pieter Martens, Steven Van Hoeylandt, Tim Du Prez, Filip E. Lynen, Frederic |
author_role |
author |
author2 |
Espeel, Pieter Martens, Steven Van Hoeylandt, Tim Du Prez, Filip E. Lynen, Frederic |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Hplc Thiol-Ene Conjugation Thiolactone Poly(N-Isopropylacrylamide) Purely Aqueous |
topic |
Hplc Thiol-Ene Conjugation Thiolactone Poly(N-Isopropylacrylamide) Purely Aqueous |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
A straightforward and efficient functionalization of aminopropylsilica with polymeric structures is described for the development of temperature responsive stationary phases applicable in purely aqueous liquid chromatography. The immobilization of the thermoresponsive polymers involves a thiolactone-based ring opening using the primary amines in aminopropylsilica, with a simultaneous one-pot, thiol-ene functionalization with an acrylate of choice. This mild, straightforward and modular grafting process results in high polymer coupling yields. By variation of the acrylate for the thiol-ene reaction, different stationary phases can be readily obtained. Two stationary phases as a result of the modular modification of aminopropylsilica were evaluated with test mixtures of hydrophobic analytes and a mixture of di- and tripeptides. Analyses using the 5 μm material packed in 10 cm x 4.6 mm columns revealed high hydrophobic retention, which proved adaptable as a function of the temperature in aqueous mobile phases. High versus low retention were obtained at temperatures above and below the lower critical solution temperature of the polymer, respectively. Moreover, the columns depict potential for diastereoisomeric peptide separation. Finally, the lower retention, observed when using PEGylated silica, illustrates the potential of the approach for modular stationary phase tuning. Fil: Satti, Angel Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (i); Argentina. University Of Ghent; Bélgica Fil: Espeel, Pieter. University Of Ghent; Bélgica Fil: Martens, Steven. University Of Ghent; Bélgica Fil: Van Hoeylandt, Tim. University Of Ghent; Bélgica Fil: Du Prez, Filip E.. University Of Ghent; Bélgica Fil: Lynen, Frederic. University Of Ghent; Bélgica |
description |
A straightforward and efficient functionalization of aminopropylsilica with polymeric structures is described for the development of temperature responsive stationary phases applicable in purely aqueous liquid chromatography. The immobilization of the thermoresponsive polymers involves a thiolactone-based ring opening using the primary amines in aminopropylsilica, with a simultaneous one-pot, thiol-ene functionalization with an acrylate of choice. This mild, straightforward and modular grafting process results in high polymer coupling yields. By variation of the acrylate for the thiol-ene reaction, different stationary phases can be readily obtained. Two stationary phases as a result of the modular modification of aminopropylsilica were evaluated with test mixtures of hydrophobic analytes and a mixture of di- and tripeptides. Analyses using the 5 μm material packed in 10 cm x 4.6 mm columns revealed high hydrophobic retention, which proved adaptable as a function of the temperature in aqueous mobile phases. High versus low retention were obtained at temperatures above and below the lower critical solution temperature of the polymer, respectively. Moreover, the columns depict potential for diastereoisomeric peptide separation. Finally, the lower retention, observed when using PEGylated silica, illustrates the potential of the approach for modular stationary phase tuning. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/4654 Satti, Angel Jose; Espeel, Pieter; Martens, Steven; Van Hoeylandt, Tim; Du Prez, Filip E.; et al.; Tunable temperature responsive liquid chromatography through thiolactone-based immobilization of poly(N-isopropylacrylamide); Elsevier Science; Journal Of Chromatography - A; 1426; 11-2015; 126-132 0021-9673 |
url |
http://hdl.handle.net/11336/4654 |
identifier_str_mv |
Satti, Angel Jose; Espeel, Pieter; Martens, Steven; Van Hoeylandt, Tim; Du Prez, Filip E.; et al.; Tunable temperature responsive liquid chromatography through thiolactone-based immobilization of poly(N-isopropylacrylamide); Elsevier Science; Journal Of Chromatography - A; 1426; 11-2015; 126-132 0021-9673 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S002196731501691X info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chroma.2015.11.063 info:eu-repo/semantics/altIdentifier/issn/0021-9673 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614483976126464 |
score |
13.070432 |