Mucoadhesive thermo-responsive chitosan-g-poly(N-isopropylacrylamide) polymeric micelles via a one-pot gamma-radiation-assisted pathway
- Autores
- Sosnik, Alejandro Dario; Imperiale, Julieta Celeste; Vázquez González, Brenda; Raskin, Maya Menaker; Muñoz Muñoz, Franklin; Burillo, Guillermina; Cedillo, Gerardo; Bucio, Emilio
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Thermo-sensitive graft copolymer amphiphiles of chitosan (CS) and poly(N-isopropylacrylamide) (PNiPAAm), CS-g-PNIPAAm, were successfully synthesized by a catalyst-less one-pot gamma (γ)-radiation-assisted free radical polymerization at three different radiation doses: 5, 10 and 20kGy. The chemical structure of the copolymers was confirmed by FTIR and solid-state 13C NMR and the grafting extent by 1H NMR and gravimetric analysis. In general, the higher the dose, the smaller the grafting due to the more significant NiPAAm homopolymerization. Due to the grafting of poly(NiPAAm) blocks, aqueous solutions of the different copolymers underwent a sharp transition upon heating above 32°C, the characteristic lower critical solution temperature (LCST) of poly(NiPAAm). Then, the critical micellar concentration (CMC), the size and size distribution and the zeta-potential were characterized by dynamic light scattering (DLS) and the polymeric micelles visualized in suspension and quantified by Nanoparticle Tracking Analysis (NTA), at 37°C. CMC values were in the 0.0012-0.0025%w/v range and micelles displayed sizes between 99 and 203nm with low polydispersity (<0.160) and highly positive zeta-potential (>+15mV) that suggested the partial conservation of the amine groups upon NiPAAm grafting. Consequently, polymeric micelles displayed the intrinsic mucoadhesiveness of CS, as established in vitro by the mucin solution assay. Finally, the encapsulation capacity of the micelles was assessed with the highly hydrophobic protease inhibitor antiretroviral indinavir free base (IDV). Polymeric micelles led to a significant 24-fold increase of the aqueous solubility from 63μg/mL to 1.45mg/mL, a performance remarkably better than different poly(ethylene oxide)-b-poly(propylene oxide) block copolymers assessed before. Overall results highlight the potential of this nanotechnology platform to expand the application of polymeric micelles to mucosal administration routes.
Fil: Sosnik, Alejandro Dario. Technion - Israel Institute of Technology; Israel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Imperiale, Julieta Celeste. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vázquez González, Brenda. Universidad Nacional Autónoma de México; México
Fil: Raskin, Maya Menaker. Technion - Israel Institute of Technology; Israel
Fil: Muñoz Muñoz, Franklin. Universidad Nacional Autónoma de México; México
Fil: Burillo, Guillermina. Universidad Nacional Autónoma de México; México
Fil: Cedillo, Gerardo. Universidad Nacional Autónoma de México; México
Fil: Bucio, Emilio. Universidad Nacional Autónoma de México; México - Materia
-
Gamma-Radiation-Assisted Free Radical Polymerization
Indinavir Free Base Encapsulation
Mucoadhesive Polymeric Micelles
Thermo-Responsive Chitosan-G-Poly(N-Isopropylacrylamide) Copolymers - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/59903
Ver los metadatos del registro completo
id |
CONICETDig_e1774b49667c2b1e30c8143598e1d2da |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/59903 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Mucoadhesive thermo-responsive chitosan-g-poly(N-isopropylacrylamide) polymeric micelles via a one-pot gamma-radiation-assisted pathwaySosnik, Alejandro DarioImperiale, Julieta CelesteVázquez González, BrendaRaskin, Maya MenakerMuñoz Muñoz, FranklinBurillo, GuillerminaCedillo, GerardoBucio, EmilioGamma-Radiation-Assisted Free Radical PolymerizationIndinavir Free Base EncapsulationMucoadhesive Polymeric MicellesThermo-Responsive Chitosan-G-Poly(N-Isopropylacrylamide) Copolymershttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Thermo-sensitive graft copolymer amphiphiles of chitosan (CS) and poly(N-isopropylacrylamide) (PNiPAAm), CS-g-PNIPAAm, were successfully synthesized by a catalyst-less one-pot gamma (γ)-radiation-assisted free radical polymerization at three different radiation doses: 5, 10 and 20kGy. The chemical structure of the copolymers was confirmed by FTIR and solid-state 13C NMR and the grafting extent by 1H NMR and gravimetric analysis. In general, the higher the dose, the smaller the grafting due to the more significant NiPAAm homopolymerization. Due to the grafting of poly(NiPAAm) blocks, aqueous solutions of the different copolymers underwent a sharp transition upon heating above 32°C, the characteristic lower critical solution temperature (LCST) of poly(NiPAAm). Then, the critical micellar concentration (CMC), the size and size distribution and the zeta-potential were characterized by dynamic light scattering (DLS) and the polymeric micelles visualized in suspension and quantified by Nanoparticle Tracking Analysis (NTA), at 37°C. CMC values were in the 0.0012-0.0025%w/v range and micelles displayed sizes between 99 and 203nm with low polydispersity (<0.160) and highly positive zeta-potential (>+15mV) that suggested the partial conservation of the amine groups upon NiPAAm grafting. Consequently, polymeric micelles displayed the intrinsic mucoadhesiveness of CS, as established in vitro by the mucin solution assay. Finally, the encapsulation capacity of the micelles was assessed with the highly hydrophobic protease inhibitor antiretroviral indinavir free base (IDV). Polymeric micelles led to a significant 24-fold increase of the aqueous solubility from 63μg/mL to 1.45mg/mL, a performance remarkably better than different poly(ethylene oxide)-b-poly(propylene oxide) block copolymers assessed before. Overall results highlight the potential of this nanotechnology platform to expand the application of polymeric micelles to mucosal administration routes.Fil: Sosnik, Alejandro Dario. Technion - Israel Institute of Technology; Israel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Imperiale, Julieta Celeste. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vázquez González, Brenda. Universidad Nacional Autónoma de México; MéxicoFil: Raskin, Maya Menaker. Technion - Israel Institute of Technology; IsraelFil: Muñoz Muñoz, Franklin. Universidad Nacional Autónoma de México; MéxicoFil: Burillo, Guillermina. Universidad Nacional Autónoma de México; MéxicoFil: Cedillo, Gerardo. Universidad Nacional Autónoma de México; MéxicoFil: Bucio, Emilio. Universidad Nacional Autónoma de México; MéxicoElsevier Science2015-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/59903Sosnik, Alejandro Dario; Imperiale, Julieta Celeste; Vázquez González, Brenda; Raskin, Maya Menaker; Muñoz Muñoz, Franklin; et al.; Mucoadhesive thermo-responsive chitosan-g-poly(N-isopropylacrylamide) polymeric micelles via a one-pot gamma-radiation-assisted pathway; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 136; 12-2015; 900-9070927-7765CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.colsurfb.2015.10.036info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0927776515302678info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:52:46Zoai:ri.conicet.gov.ar:11336/59903instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:52:46.648CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Mucoadhesive thermo-responsive chitosan-g-poly(N-isopropylacrylamide) polymeric micelles via a one-pot gamma-radiation-assisted pathway |
title |
Mucoadhesive thermo-responsive chitosan-g-poly(N-isopropylacrylamide) polymeric micelles via a one-pot gamma-radiation-assisted pathway |
spellingShingle |
Mucoadhesive thermo-responsive chitosan-g-poly(N-isopropylacrylamide) polymeric micelles via a one-pot gamma-radiation-assisted pathway Sosnik, Alejandro Dario Gamma-Radiation-Assisted Free Radical Polymerization Indinavir Free Base Encapsulation Mucoadhesive Polymeric Micelles Thermo-Responsive Chitosan-G-Poly(N-Isopropylacrylamide) Copolymers |
title_short |
Mucoadhesive thermo-responsive chitosan-g-poly(N-isopropylacrylamide) polymeric micelles via a one-pot gamma-radiation-assisted pathway |
title_full |
Mucoadhesive thermo-responsive chitosan-g-poly(N-isopropylacrylamide) polymeric micelles via a one-pot gamma-radiation-assisted pathway |
title_fullStr |
Mucoadhesive thermo-responsive chitosan-g-poly(N-isopropylacrylamide) polymeric micelles via a one-pot gamma-radiation-assisted pathway |
title_full_unstemmed |
Mucoadhesive thermo-responsive chitosan-g-poly(N-isopropylacrylamide) polymeric micelles via a one-pot gamma-radiation-assisted pathway |
title_sort |
Mucoadhesive thermo-responsive chitosan-g-poly(N-isopropylacrylamide) polymeric micelles via a one-pot gamma-radiation-assisted pathway |
dc.creator.none.fl_str_mv |
Sosnik, Alejandro Dario Imperiale, Julieta Celeste Vázquez González, Brenda Raskin, Maya Menaker Muñoz Muñoz, Franklin Burillo, Guillermina Cedillo, Gerardo Bucio, Emilio |
author |
Sosnik, Alejandro Dario |
author_facet |
Sosnik, Alejandro Dario Imperiale, Julieta Celeste Vázquez González, Brenda Raskin, Maya Menaker Muñoz Muñoz, Franklin Burillo, Guillermina Cedillo, Gerardo Bucio, Emilio |
author_role |
author |
author2 |
Imperiale, Julieta Celeste Vázquez González, Brenda Raskin, Maya Menaker Muñoz Muñoz, Franklin Burillo, Guillermina Cedillo, Gerardo Bucio, Emilio |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
Gamma-Radiation-Assisted Free Radical Polymerization Indinavir Free Base Encapsulation Mucoadhesive Polymeric Micelles Thermo-Responsive Chitosan-G-Poly(N-Isopropylacrylamide) Copolymers |
topic |
Gamma-Radiation-Assisted Free Radical Polymerization Indinavir Free Base Encapsulation Mucoadhesive Polymeric Micelles Thermo-Responsive Chitosan-G-Poly(N-Isopropylacrylamide) Copolymers |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.10 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Thermo-sensitive graft copolymer amphiphiles of chitosan (CS) and poly(N-isopropylacrylamide) (PNiPAAm), CS-g-PNIPAAm, were successfully synthesized by a catalyst-less one-pot gamma (γ)-radiation-assisted free radical polymerization at three different radiation doses: 5, 10 and 20kGy. The chemical structure of the copolymers was confirmed by FTIR and solid-state 13C NMR and the grafting extent by 1H NMR and gravimetric analysis. In general, the higher the dose, the smaller the grafting due to the more significant NiPAAm homopolymerization. Due to the grafting of poly(NiPAAm) blocks, aqueous solutions of the different copolymers underwent a sharp transition upon heating above 32°C, the characteristic lower critical solution temperature (LCST) of poly(NiPAAm). Then, the critical micellar concentration (CMC), the size and size distribution and the zeta-potential were characterized by dynamic light scattering (DLS) and the polymeric micelles visualized in suspension and quantified by Nanoparticle Tracking Analysis (NTA), at 37°C. CMC values were in the 0.0012-0.0025%w/v range and micelles displayed sizes between 99 and 203nm with low polydispersity (<0.160) and highly positive zeta-potential (>+15mV) that suggested the partial conservation of the amine groups upon NiPAAm grafting. Consequently, polymeric micelles displayed the intrinsic mucoadhesiveness of CS, as established in vitro by the mucin solution assay. Finally, the encapsulation capacity of the micelles was assessed with the highly hydrophobic protease inhibitor antiretroviral indinavir free base (IDV). Polymeric micelles led to a significant 24-fold increase of the aqueous solubility from 63μg/mL to 1.45mg/mL, a performance remarkably better than different poly(ethylene oxide)-b-poly(propylene oxide) block copolymers assessed before. Overall results highlight the potential of this nanotechnology platform to expand the application of polymeric micelles to mucosal administration routes. Fil: Sosnik, Alejandro Dario. Technion - Israel Institute of Technology; Israel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Imperiale, Julieta Celeste. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Vázquez González, Brenda. Universidad Nacional Autónoma de México; México Fil: Raskin, Maya Menaker. Technion - Israel Institute of Technology; Israel Fil: Muñoz Muñoz, Franklin. Universidad Nacional Autónoma de México; México Fil: Burillo, Guillermina. Universidad Nacional Autónoma de México; México Fil: Cedillo, Gerardo. Universidad Nacional Autónoma de México; México Fil: Bucio, Emilio. Universidad Nacional Autónoma de México; México |
description |
Thermo-sensitive graft copolymer amphiphiles of chitosan (CS) and poly(N-isopropylacrylamide) (PNiPAAm), CS-g-PNIPAAm, were successfully synthesized by a catalyst-less one-pot gamma (γ)-radiation-assisted free radical polymerization at three different radiation doses: 5, 10 and 20kGy. The chemical structure of the copolymers was confirmed by FTIR and solid-state 13C NMR and the grafting extent by 1H NMR and gravimetric analysis. In general, the higher the dose, the smaller the grafting due to the more significant NiPAAm homopolymerization. Due to the grafting of poly(NiPAAm) blocks, aqueous solutions of the different copolymers underwent a sharp transition upon heating above 32°C, the characteristic lower critical solution temperature (LCST) of poly(NiPAAm). Then, the critical micellar concentration (CMC), the size and size distribution and the zeta-potential were characterized by dynamic light scattering (DLS) and the polymeric micelles visualized in suspension and quantified by Nanoparticle Tracking Analysis (NTA), at 37°C. CMC values were in the 0.0012-0.0025%w/v range and micelles displayed sizes between 99 and 203nm with low polydispersity (<0.160) and highly positive zeta-potential (>+15mV) that suggested the partial conservation of the amine groups upon NiPAAm grafting. Consequently, polymeric micelles displayed the intrinsic mucoadhesiveness of CS, as established in vitro by the mucin solution assay. Finally, the encapsulation capacity of the micelles was assessed with the highly hydrophobic protease inhibitor antiretroviral indinavir free base (IDV). Polymeric micelles led to a significant 24-fold increase of the aqueous solubility from 63μg/mL to 1.45mg/mL, a performance remarkably better than different poly(ethylene oxide)-b-poly(propylene oxide) block copolymers assessed before. Overall results highlight the potential of this nanotechnology platform to expand the application of polymeric micelles to mucosal administration routes. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/59903 Sosnik, Alejandro Dario; Imperiale, Julieta Celeste; Vázquez González, Brenda; Raskin, Maya Menaker; Muñoz Muñoz, Franklin; et al.; Mucoadhesive thermo-responsive chitosan-g-poly(N-isopropylacrylamide) polymeric micelles via a one-pot gamma-radiation-assisted pathway; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 136; 12-2015; 900-907 0927-7765 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/59903 |
identifier_str_mv |
Sosnik, Alejandro Dario; Imperiale, Julieta Celeste; Vázquez González, Brenda; Raskin, Maya Menaker; Muñoz Muñoz, Franklin; et al.; Mucoadhesive thermo-responsive chitosan-g-poly(N-isopropylacrylamide) polymeric micelles via a one-pot gamma-radiation-assisted pathway; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 136; 12-2015; 900-907 0927-7765 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.colsurfb.2015.10.036 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0927776515302678 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613617226350592 |
score |
13.070432 |