Mucoadhesive thermo-responsive chitosan-g-poly(N-isopropylacrylamide) polymeric micelles via a one-pot gamma-radiation-assisted pathway

Autores
Sosnik, Alejandro Dario; Imperiale, Julieta Celeste; Vázquez González, Brenda; Raskin, Maya Menaker; Muñoz Muñoz, Franklin; Burillo, Guillermina; Cedillo, Gerardo; Bucio, Emilio
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Thermo-sensitive graft copolymer amphiphiles of chitosan (CS) and poly(N-isopropylacrylamide) (PNiPAAm), CS-g-PNIPAAm, were successfully synthesized by a catalyst-less one-pot gamma (γ)-radiation-assisted free radical polymerization at three different radiation doses: 5, 10 and 20kGy. The chemical structure of the copolymers was confirmed by FTIR and solid-state 13C NMR and the grafting extent by 1H NMR and gravimetric analysis. In general, the higher the dose, the smaller the grafting due to the more significant NiPAAm homopolymerization. Due to the grafting of poly(NiPAAm) blocks, aqueous solutions of the different copolymers underwent a sharp transition upon heating above 32°C, the characteristic lower critical solution temperature (LCST) of poly(NiPAAm). Then, the critical micellar concentration (CMC), the size and size distribution and the zeta-potential were characterized by dynamic light scattering (DLS) and the polymeric micelles visualized in suspension and quantified by Nanoparticle Tracking Analysis (NTA), at 37°C. CMC values were in the 0.0012-0.0025%w/v range and micelles displayed sizes between 99 and 203nm with low polydispersity (<0.160) and highly positive zeta-potential (>+15mV) that suggested the partial conservation of the amine groups upon NiPAAm grafting. Consequently, polymeric micelles displayed the intrinsic mucoadhesiveness of CS, as established in vitro by the mucin solution assay. Finally, the encapsulation capacity of the micelles was assessed with the highly hydrophobic protease inhibitor antiretroviral indinavir free base (IDV). Polymeric micelles led to a significant 24-fold increase of the aqueous solubility from 63μg/mL to 1.45mg/mL, a performance remarkably better than different poly(ethylene oxide)-b-poly(propylene oxide) block copolymers assessed before. Overall results highlight the potential of this nanotechnology platform to expand the application of polymeric micelles to mucosal administration routes.
Fil: Sosnik, Alejandro Dario. Technion - Israel Institute of Technology; Israel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Imperiale, Julieta Celeste. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vázquez González, Brenda. Universidad Nacional Autónoma de México; México
Fil: Raskin, Maya Menaker. Technion - Israel Institute of Technology; Israel
Fil: Muñoz Muñoz, Franklin. Universidad Nacional Autónoma de México; México
Fil: Burillo, Guillermina. Universidad Nacional Autónoma de México; México
Fil: Cedillo, Gerardo. Universidad Nacional Autónoma de México; México
Fil: Bucio, Emilio. Universidad Nacional Autónoma de México; México
Materia
Gamma-Radiation-Assisted Free Radical Polymerization
Indinavir Free Base Encapsulation
Mucoadhesive Polymeric Micelles
Thermo-Responsive Chitosan-G-Poly(N-Isopropylacrylamide) Copolymers
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/59903

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network_name_str CONICET Digital (CONICET)
spelling Mucoadhesive thermo-responsive chitosan-g-poly(N-isopropylacrylamide) polymeric micelles via a one-pot gamma-radiation-assisted pathwaySosnik, Alejandro DarioImperiale, Julieta CelesteVázquez González, BrendaRaskin, Maya MenakerMuñoz Muñoz, FranklinBurillo, GuillerminaCedillo, GerardoBucio, EmilioGamma-Radiation-Assisted Free Radical PolymerizationIndinavir Free Base EncapsulationMucoadhesive Polymeric MicellesThermo-Responsive Chitosan-G-Poly(N-Isopropylacrylamide) Copolymershttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Thermo-sensitive graft copolymer amphiphiles of chitosan (CS) and poly(N-isopropylacrylamide) (PNiPAAm), CS-g-PNIPAAm, were successfully synthesized by a catalyst-less one-pot gamma (γ)-radiation-assisted free radical polymerization at three different radiation doses: 5, 10 and 20kGy. The chemical structure of the copolymers was confirmed by FTIR and solid-state 13C NMR and the grafting extent by 1H NMR and gravimetric analysis. In general, the higher the dose, the smaller the grafting due to the more significant NiPAAm homopolymerization. Due to the grafting of poly(NiPAAm) blocks, aqueous solutions of the different copolymers underwent a sharp transition upon heating above 32°C, the characteristic lower critical solution temperature (LCST) of poly(NiPAAm). Then, the critical micellar concentration (CMC), the size and size distribution and the zeta-potential were characterized by dynamic light scattering (DLS) and the polymeric micelles visualized in suspension and quantified by Nanoparticle Tracking Analysis (NTA), at 37°C. CMC values were in the 0.0012-0.0025%w/v range and micelles displayed sizes between 99 and 203nm with low polydispersity (<0.160) and highly positive zeta-potential (>+15mV) that suggested the partial conservation of the amine groups upon NiPAAm grafting. Consequently, polymeric micelles displayed the intrinsic mucoadhesiveness of CS, as established in vitro by the mucin solution assay. Finally, the encapsulation capacity of the micelles was assessed with the highly hydrophobic protease inhibitor antiretroviral indinavir free base (IDV). Polymeric micelles led to a significant 24-fold increase of the aqueous solubility from 63μg/mL to 1.45mg/mL, a performance remarkably better than different poly(ethylene oxide)-b-poly(propylene oxide) block copolymers assessed before. Overall results highlight the potential of this nanotechnology platform to expand the application of polymeric micelles to mucosal administration routes.Fil: Sosnik, Alejandro Dario. Technion - Israel Institute of Technology; Israel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Imperiale, Julieta Celeste. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vázquez González, Brenda. Universidad Nacional Autónoma de México; MéxicoFil: Raskin, Maya Menaker. Technion - Israel Institute of Technology; IsraelFil: Muñoz Muñoz, Franklin. Universidad Nacional Autónoma de México; MéxicoFil: Burillo, Guillermina. Universidad Nacional Autónoma de México; MéxicoFil: Cedillo, Gerardo. Universidad Nacional Autónoma de México; MéxicoFil: Bucio, Emilio. Universidad Nacional Autónoma de México; MéxicoElsevier Science2015-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/59903Sosnik, Alejandro Dario; Imperiale, Julieta Celeste; Vázquez González, Brenda; Raskin, Maya Menaker; Muñoz Muñoz, Franklin; et al.; Mucoadhesive thermo-responsive chitosan-g-poly(N-isopropylacrylamide) polymeric micelles via a one-pot gamma-radiation-assisted pathway; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 136; 12-2015; 900-9070927-7765CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.colsurfb.2015.10.036info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0927776515302678info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:52:46Zoai:ri.conicet.gov.ar:11336/59903instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:52:46.648CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mucoadhesive thermo-responsive chitosan-g-poly(N-isopropylacrylamide) polymeric micelles via a one-pot gamma-radiation-assisted pathway
title Mucoadhesive thermo-responsive chitosan-g-poly(N-isopropylacrylamide) polymeric micelles via a one-pot gamma-radiation-assisted pathway
spellingShingle Mucoadhesive thermo-responsive chitosan-g-poly(N-isopropylacrylamide) polymeric micelles via a one-pot gamma-radiation-assisted pathway
Sosnik, Alejandro Dario
Gamma-Radiation-Assisted Free Radical Polymerization
Indinavir Free Base Encapsulation
Mucoadhesive Polymeric Micelles
Thermo-Responsive Chitosan-G-Poly(N-Isopropylacrylamide) Copolymers
title_short Mucoadhesive thermo-responsive chitosan-g-poly(N-isopropylacrylamide) polymeric micelles via a one-pot gamma-radiation-assisted pathway
title_full Mucoadhesive thermo-responsive chitosan-g-poly(N-isopropylacrylamide) polymeric micelles via a one-pot gamma-radiation-assisted pathway
title_fullStr Mucoadhesive thermo-responsive chitosan-g-poly(N-isopropylacrylamide) polymeric micelles via a one-pot gamma-radiation-assisted pathway
title_full_unstemmed Mucoadhesive thermo-responsive chitosan-g-poly(N-isopropylacrylamide) polymeric micelles via a one-pot gamma-radiation-assisted pathway
title_sort Mucoadhesive thermo-responsive chitosan-g-poly(N-isopropylacrylamide) polymeric micelles via a one-pot gamma-radiation-assisted pathway
dc.creator.none.fl_str_mv Sosnik, Alejandro Dario
Imperiale, Julieta Celeste
Vázquez González, Brenda
Raskin, Maya Menaker
Muñoz Muñoz, Franklin
Burillo, Guillermina
Cedillo, Gerardo
Bucio, Emilio
author Sosnik, Alejandro Dario
author_facet Sosnik, Alejandro Dario
Imperiale, Julieta Celeste
Vázquez González, Brenda
Raskin, Maya Menaker
Muñoz Muñoz, Franklin
Burillo, Guillermina
Cedillo, Gerardo
Bucio, Emilio
author_role author
author2 Imperiale, Julieta Celeste
Vázquez González, Brenda
Raskin, Maya Menaker
Muñoz Muñoz, Franklin
Burillo, Guillermina
Cedillo, Gerardo
Bucio, Emilio
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Gamma-Radiation-Assisted Free Radical Polymerization
Indinavir Free Base Encapsulation
Mucoadhesive Polymeric Micelles
Thermo-Responsive Chitosan-G-Poly(N-Isopropylacrylamide) Copolymers
topic Gamma-Radiation-Assisted Free Radical Polymerization
Indinavir Free Base Encapsulation
Mucoadhesive Polymeric Micelles
Thermo-Responsive Chitosan-G-Poly(N-Isopropylacrylamide) Copolymers
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Thermo-sensitive graft copolymer amphiphiles of chitosan (CS) and poly(N-isopropylacrylamide) (PNiPAAm), CS-g-PNIPAAm, were successfully synthesized by a catalyst-less one-pot gamma (γ)-radiation-assisted free radical polymerization at three different radiation doses: 5, 10 and 20kGy. The chemical structure of the copolymers was confirmed by FTIR and solid-state 13C NMR and the grafting extent by 1H NMR and gravimetric analysis. In general, the higher the dose, the smaller the grafting due to the more significant NiPAAm homopolymerization. Due to the grafting of poly(NiPAAm) blocks, aqueous solutions of the different copolymers underwent a sharp transition upon heating above 32°C, the characteristic lower critical solution temperature (LCST) of poly(NiPAAm). Then, the critical micellar concentration (CMC), the size and size distribution and the zeta-potential were characterized by dynamic light scattering (DLS) and the polymeric micelles visualized in suspension and quantified by Nanoparticle Tracking Analysis (NTA), at 37°C. CMC values were in the 0.0012-0.0025%w/v range and micelles displayed sizes between 99 and 203nm with low polydispersity (<0.160) and highly positive zeta-potential (>+15mV) that suggested the partial conservation of the amine groups upon NiPAAm grafting. Consequently, polymeric micelles displayed the intrinsic mucoadhesiveness of CS, as established in vitro by the mucin solution assay. Finally, the encapsulation capacity of the micelles was assessed with the highly hydrophobic protease inhibitor antiretroviral indinavir free base (IDV). Polymeric micelles led to a significant 24-fold increase of the aqueous solubility from 63μg/mL to 1.45mg/mL, a performance remarkably better than different poly(ethylene oxide)-b-poly(propylene oxide) block copolymers assessed before. Overall results highlight the potential of this nanotechnology platform to expand the application of polymeric micelles to mucosal administration routes.
Fil: Sosnik, Alejandro Dario. Technion - Israel Institute of Technology; Israel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Imperiale, Julieta Celeste. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vázquez González, Brenda. Universidad Nacional Autónoma de México; México
Fil: Raskin, Maya Menaker. Technion - Israel Institute of Technology; Israel
Fil: Muñoz Muñoz, Franklin. Universidad Nacional Autónoma de México; México
Fil: Burillo, Guillermina. Universidad Nacional Autónoma de México; México
Fil: Cedillo, Gerardo. Universidad Nacional Autónoma de México; México
Fil: Bucio, Emilio. Universidad Nacional Autónoma de México; México
description Thermo-sensitive graft copolymer amphiphiles of chitosan (CS) and poly(N-isopropylacrylamide) (PNiPAAm), CS-g-PNIPAAm, were successfully synthesized by a catalyst-less one-pot gamma (γ)-radiation-assisted free radical polymerization at three different radiation doses: 5, 10 and 20kGy. The chemical structure of the copolymers was confirmed by FTIR and solid-state 13C NMR and the grafting extent by 1H NMR and gravimetric analysis. In general, the higher the dose, the smaller the grafting due to the more significant NiPAAm homopolymerization. Due to the grafting of poly(NiPAAm) blocks, aqueous solutions of the different copolymers underwent a sharp transition upon heating above 32°C, the characteristic lower critical solution temperature (LCST) of poly(NiPAAm). Then, the critical micellar concentration (CMC), the size and size distribution and the zeta-potential were characterized by dynamic light scattering (DLS) and the polymeric micelles visualized in suspension and quantified by Nanoparticle Tracking Analysis (NTA), at 37°C. CMC values were in the 0.0012-0.0025%w/v range and micelles displayed sizes between 99 and 203nm with low polydispersity (<0.160) and highly positive zeta-potential (>+15mV) that suggested the partial conservation of the amine groups upon NiPAAm grafting. Consequently, polymeric micelles displayed the intrinsic mucoadhesiveness of CS, as established in vitro by the mucin solution assay. Finally, the encapsulation capacity of the micelles was assessed with the highly hydrophobic protease inhibitor antiretroviral indinavir free base (IDV). Polymeric micelles led to a significant 24-fold increase of the aqueous solubility from 63μg/mL to 1.45mg/mL, a performance remarkably better than different poly(ethylene oxide)-b-poly(propylene oxide) block copolymers assessed before. Overall results highlight the potential of this nanotechnology platform to expand the application of polymeric micelles to mucosal administration routes.
publishDate 2015
dc.date.none.fl_str_mv 2015-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/59903
Sosnik, Alejandro Dario; Imperiale, Julieta Celeste; Vázquez González, Brenda; Raskin, Maya Menaker; Muñoz Muñoz, Franklin; et al.; Mucoadhesive thermo-responsive chitosan-g-poly(N-isopropylacrylamide) polymeric micelles via a one-pot gamma-radiation-assisted pathway; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 136; 12-2015; 900-907
0927-7765
CONICET Digital
CONICET
url http://hdl.handle.net/11336/59903
identifier_str_mv Sosnik, Alejandro Dario; Imperiale, Julieta Celeste; Vázquez González, Brenda; Raskin, Maya Menaker; Muñoz Muñoz, Franklin; et al.; Mucoadhesive thermo-responsive chitosan-g-poly(N-isopropylacrylamide) polymeric micelles via a one-pot gamma-radiation-assisted pathway; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 136; 12-2015; 900-907
0927-7765
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.colsurfb.2015.10.036
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0927776515302678
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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