Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmission

Autores
Castells, Matías; Caffarena, Rubén Darío; Casaux, María Laura; Schild, Carlos; Miño, Samue; Castells, Felipe; Castells, Daniel; Victoria, Matías; Riet Correa, Franklin; Giannitti, Federico; Parreño, Gladys Viviana; Colina, Rodney
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Uruguay is one of the main exporters of beef and dairy products, and cattle production is one of the main economic sectors in this country. Rotavirus A (RVA) is the main pathogen associated with neonatal calf diarrhea (NCD), a syndrome that leads to significant economic losses to the livestock industry. The aims of this study are to determine the frequency of RVA infections, and to analyze the genetic diversity of RVA strains in calves in Uruguay. A total of 833 samples from dairy and beef calves were analyzed through RT-qPCR and sequencing. RVA was detected in 57.0% of the samples. The frequency of detection was significantly higher in dairy (59.5%) than beef (28.4%) calves (p < 0.001), while it did not differ significantly among calves born in herds that were vaccinated (64.0%) or not vaccinated (66.7%) against NCD. The frequency of RVA detection and the viral load were significantly higher in samples from diarrheic (72.1%, 7.99 log10 genome copies/mL of feces) than non-diarrheic (59.9%, 7.35 log10 genome copies/mL of feces) calves (p < 0.005 and p = 0.007, respectively). The observed G-types (VP7) were G6 (77.6%), G10 (20.7%), and G24 (1.7%), while the P-types were P[5] (28.4%), P[11] (70.7%), and P[33] (0.9%). The G-type and P-type combinations were G6P[11] (40.4%), G6P[5] (38.6%), G10P[11] (19.3%), and the uncommon genotype G24P[33] (1.8%). VP6 and NSP1-5 genotyping were performed to better characterize some strains. The phylogenetic analyses suggested interspecies transmission, including transmission between animals and humans.
Fil: Castells, Matías. Universidad de la República; Uruguay
Fil: Caffarena, Rubén Darío. Universidad de la República; Uruguay
Fil: Casaux, María Laura. Universidad de la República; Uruguay
Fil: Schild, Carlos. Universidad de la República; Uruguay
Fil: Miño, Samue. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Castells, Felipe. Universidad de la República; Uruguay
Fil: Castells, Daniel. Universidad de la República; Uruguay
Fil: Victoria, Matías. Universidad de la República; Uruguay
Fil: Riet Correa, Franklin. Universidad de la República; Uruguay
Fil: Giannitti, Federico. Universidad de la República; Uruguay
Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Virología E Innovaciones Tecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Virología E Innovaciones Tecnológicas; Argentina
Fil: Colina, Rodney. Universidad de la República; Uruguay
Materia
BOVINE
DIARRHEA
GENOTYPES
INTERSPECIES TRANSMISSION
ROTAVIRUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/150783
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/150783
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmissionCastells, MatíasCaffarena, Rubén DaríoCasaux, María LauraSchild, CarlosMiño, SamueCastells, FelipeCastells, DanielVictoria, MatíasRiet Correa, FranklinGiannitti, FedericoParreño, Gladys VivianaColina, RodneyBOVINEDIARRHEAGENOTYPESINTERSPECIES TRANSMISSIONROTAVIRUShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Uruguay is one of the main exporters of beef and dairy products, and cattle production is one of the main economic sectors in this country. Rotavirus A (RVA) is the main pathogen associated with neonatal calf diarrhea (NCD), a syndrome that leads to significant economic losses to the livestock industry. The aims of this study are to determine the frequency of RVA infections, and to analyze the genetic diversity of RVA strains in calves in Uruguay. A total of 833 samples from dairy and beef calves were analyzed through RT-qPCR and sequencing. RVA was detected in 57.0% of the samples. The frequency of detection was significantly higher in dairy (59.5%) than beef (28.4%) calves (p < 0.001), while it did not differ significantly among calves born in herds that were vaccinated (64.0%) or not vaccinated (66.7%) against NCD. The frequency of RVA detection and the viral load were significantly higher in samples from diarrheic (72.1%, 7.99 log10 genome copies/mL of feces) than non-diarrheic (59.9%, 7.35 log10 genome copies/mL of feces) calves (p < 0.005 and p = 0.007, respectively). The observed G-types (VP7) were G6 (77.6%), G10 (20.7%), and G24 (1.7%), while the P-types were P[5] (28.4%), P[11] (70.7%), and P[33] (0.9%). The G-type and P-type combinations were G6P[11] (40.4%), G6P[5] (38.6%), G10P[11] (19.3%), and the uncommon genotype G24P[33] (1.8%). VP6 and NSP1-5 genotyping were performed to better characterize some strains. The phylogenetic analyses suggested interspecies transmission, including transmission between animals and humans.Fil: Castells, Matías. Universidad de la República; UruguayFil: Caffarena, Rubén Darío. Universidad de la República; UruguayFil: Casaux, María Laura. Universidad de la República; UruguayFil: Schild, Carlos. Universidad de la República; UruguayFil: Miño, Samue. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Castells, Felipe. Universidad de la República; UruguayFil: Castells, Daniel. Universidad de la República; UruguayFil: Victoria, Matías. Universidad de la República; UruguayFil: Riet Correa, Franklin. Universidad de la República; UruguayFil: Giannitti, Federico. Universidad de la República; UruguayFil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Virología E Innovaciones Tecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Virología E Innovaciones Tecnológicas; ArgentinaFil: Colina, Rodney. Universidad de la República; UruguayMolecular Diversity Preservation International2020-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/150783Castells, Matías; Caffarena, Rubén Darío; Casaux, María Laura; Schild, Carlos; Miño, Samue; et al.; Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmission; Molecular Diversity Preservation International; Pathogens; 9; 7; 7-2020; 1-172076-0817CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/pathogens9070570info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:22Zoai:ri.conicet.gov.ar:11336/150783instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:22.298CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmission
title Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmission
spellingShingle Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmission
Castells, Matías
BOVINE
DIARRHEA
GENOTYPES
INTERSPECIES TRANSMISSION
ROTAVIRUS
title_short Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmission
title_full Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmission
title_fullStr Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmission
title_full_unstemmed Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmission
title_sort Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmission
dc.creator.none.fl_str_mv Castells, Matías
Caffarena, Rubén Darío
Casaux, María Laura
Schild, Carlos
Miño, Samue
Castells, Felipe
Castells, Daniel
Victoria, Matías
Riet Correa, Franklin
Giannitti, Federico
Parreño, Gladys Viviana
Colina, Rodney
author Castells, Matías
author_facet Castells, Matías
Caffarena, Rubén Darío
Casaux, María Laura
Schild, Carlos
Miño, Samue
Castells, Felipe
Castells, Daniel
Victoria, Matías
Riet Correa, Franklin
Giannitti, Federico
Parreño, Gladys Viviana
Colina, Rodney
author_role author
author2 Caffarena, Rubén Darío
Casaux, María Laura
Schild, Carlos
Miño, Samue
Castells, Felipe
Castells, Daniel
Victoria, Matías
Riet Correa, Franklin
Giannitti, Federico
Parreño, Gladys Viviana
Colina, Rodney
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BOVINE
DIARRHEA
GENOTYPES
INTERSPECIES TRANSMISSION
ROTAVIRUS
topic BOVINE
DIARRHEA
GENOTYPES
INTERSPECIES TRANSMISSION
ROTAVIRUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Uruguay is one of the main exporters of beef and dairy products, and cattle production is one of the main economic sectors in this country. Rotavirus A (RVA) is the main pathogen associated with neonatal calf diarrhea (NCD), a syndrome that leads to significant economic losses to the livestock industry. The aims of this study are to determine the frequency of RVA infections, and to analyze the genetic diversity of RVA strains in calves in Uruguay. A total of 833 samples from dairy and beef calves were analyzed through RT-qPCR and sequencing. RVA was detected in 57.0% of the samples. The frequency of detection was significantly higher in dairy (59.5%) than beef (28.4%) calves (p < 0.001), while it did not differ significantly among calves born in herds that were vaccinated (64.0%) or not vaccinated (66.7%) against NCD. The frequency of RVA detection and the viral load were significantly higher in samples from diarrheic (72.1%, 7.99 log10 genome copies/mL of feces) than non-diarrheic (59.9%, 7.35 log10 genome copies/mL of feces) calves (p < 0.005 and p = 0.007, respectively). The observed G-types (VP7) were G6 (77.6%), G10 (20.7%), and G24 (1.7%), while the P-types were P[5] (28.4%), P[11] (70.7%), and P[33] (0.9%). The G-type and P-type combinations were G6P[11] (40.4%), G6P[5] (38.6%), G10P[11] (19.3%), and the uncommon genotype G24P[33] (1.8%). VP6 and NSP1-5 genotyping were performed to better characterize some strains. The phylogenetic analyses suggested interspecies transmission, including transmission between animals and humans.
Fil: Castells, Matías. Universidad de la República; Uruguay
Fil: Caffarena, Rubén Darío. Universidad de la República; Uruguay
Fil: Casaux, María Laura. Universidad de la República; Uruguay
Fil: Schild, Carlos. Universidad de la República; Uruguay
Fil: Miño, Samue. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Castells, Felipe. Universidad de la República; Uruguay
Fil: Castells, Daniel. Universidad de la República; Uruguay
Fil: Victoria, Matías. Universidad de la República; Uruguay
Fil: Riet Correa, Franklin. Universidad de la República; Uruguay
Fil: Giannitti, Federico. Universidad de la República; Uruguay
Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Virología E Innovaciones Tecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Virología E Innovaciones Tecnológicas; Argentina
Fil: Colina, Rodney. Universidad de la República; Uruguay
description Uruguay is one of the main exporters of beef and dairy products, and cattle production is one of the main economic sectors in this country. Rotavirus A (RVA) is the main pathogen associated with neonatal calf diarrhea (NCD), a syndrome that leads to significant economic losses to the livestock industry. The aims of this study are to determine the frequency of RVA infections, and to analyze the genetic diversity of RVA strains in calves in Uruguay. A total of 833 samples from dairy and beef calves were analyzed through RT-qPCR and sequencing. RVA was detected in 57.0% of the samples. The frequency of detection was significantly higher in dairy (59.5%) than beef (28.4%) calves (p < 0.001), while it did not differ significantly among calves born in herds that were vaccinated (64.0%) or not vaccinated (66.7%) against NCD. The frequency of RVA detection and the viral load were significantly higher in samples from diarrheic (72.1%, 7.99 log10 genome copies/mL of feces) than non-diarrheic (59.9%, 7.35 log10 genome copies/mL of feces) calves (p < 0.005 and p = 0.007, respectively). The observed G-types (VP7) were G6 (77.6%), G10 (20.7%), and G24 (1.7%), while the P-types were P[5] (28.4%), P[11] (70.7%), and P[33] (0.9%). The G-type and P-type combinations were G6P[11] (40.4%), G6P[5] (38.6%), G10P[11] (19.3%), and the uncommon genotype G24P[33] (1.8%). VP6 and NSP1-5 genotyping were performed to better characterize some strains. The phylogenetic analyses suggested interspecies transmission, including transmission between animals and humans.
publishDate 2020
dc.date.none.fl_str_mv 2020-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/150783
Castells, Matías; Caffarena, Rubén Darío; Casaux, María Laura; Schild, Carlos; Miño, Samue; et al.; Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmission; Molecular Diversity Preservation International; Pathogens; 9; 7; 7-2020; 1-17
2076-0817
CONICET Digital
CONICET
url http://hdl.handle.net/11336/150783
identifier_str_mv Castells, Matías; Caffarena, Rubén Darío; Casaux, María Laura; Schild, Carlos; Miño, Samue; et al.; Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmission; Molecular Diversity Preservation International; Pathogens; 9; 7; 7-2020; 1-17
2076-0817
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/pathogens9070570
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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