Intestinal antispasmodic effects of three argentinian plants: Hypericum connatum, Berberis ruscifolia and Cecropia pachystachya: mechanisms of action and comparison with the effect...
- Autores
- Matera, Soledad Inés; Bruno, F.; Perez, Vanina Andrea; Querini, C.; Ragone, María Inés; Consolini, Alicia Elvira; Bayley, Matias
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Some medicinal plants are used in Argentina to reduce gastrointestinal symptoms as antispasmodics, but notall these effects were validated. This work studied the effects of three native plants as the ethanolic tinctures(T) of Hypericum connatum and Berberis ruscifolia, as well as the acqueous crude extracts (A.c.e) of Cecropiapachystachya, and compared the mechanisms with those of the peruvian plant Brugmansia arborea inisolated rat intestines. The first three plants demonstrated to have antispasmodic effect mainly due to thenon-competitive blockade of the agonist-induced contraction and calcium influx to the smooth muscle,which was associated to the presence of flavonoids. H. connatum also induced the release of prostaglandinswhich cause intestinal contraction, and NO which cause peristalsis, in a way that the blockade of bothmechanisms potentiated the relaxant effect of the T-H.c. In contrast, the A.c.e of Brugmansia arboreashowed to be a competitive antagonist of muscarinic receptors in the isolated intestine, in agreement withthe presence of tropane alkaloids. Moreover, H. connatum and C. pachystachya also produced certainsedation, which could contribute to the antispasmodic effect. The tincture of Hypericum connatum, but notthat of Berberis ruscifolia, reduced the spontaneous locomotion and exploration of mice in the open-fieldtest, at doses of 200 mg leaves/Kg. Results suggest that the three native plants exhibited an importantantispasmodic effect mainly due to non-competitive antagonism of the agonist and of Ca2+-influx to smoothmuscle.
Fil: Matera, Soledad Inés. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
Fil: Bruno, F.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
Fil: Perez, Vanina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
Fil: Querini, C.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
Fil: Ragone, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
Fil: Consolini, Alicia Elvira. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
Fil: Bayley, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina - Materia
-
Hypericum Connatum
Berberis Ruscifolia
Cecropia Pachystachya
Brugmansia Arborea
Antispasmodic
Ca2+-Blockade
Sedative - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/55929
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Intestinal antispasmodic effects of three argentinian plants: Hypericum connatum, Berberis ruscifolia and Cecropia pachystachya: mechanisms of action and comparison with the effects of Brugmansia arboreaMatera, Soledad InésBruno, F.Perez, Vanina AndreaQuerini, C.Ragone, María InésConsolini, Alicia ElviraBayley, MatiasHypericum ConnatumBerberis RuscifoliaCecropia PachystachyaBrugmansia ArboreaAntispasmodicCa2+-BlockadeSedativehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Some medicinal plants are used in Argentina to reduce gastrointestinal symptoms as antispasmodics, but notall these effects were validated. This work studied the effects of three native plants as the ethanolic tinctures(T) of Hypericum connatum and Berberis ruscifolia, as well as the acqueous crude extracts (A.c.e) of Cecropiapachystachya, and compared the mechanisms with those of the peruvian plant Brugmansia arborea inisolated rat intestines. The first three plants demonstrated to have antispasmodic effect mainly due to thenon-competitive blockade of the agonist-induced contraction and calcium influx to the smooth muscle,which was associated to the presence of flavonoids. H. connatum also induced the release of prostaglandinswhich cause intestinal contraction, and NO which cause peristalsis, in a way that the blockade of bothmechanisms potentiated the relaxant effect of the T-H.c. In contrast, the A.c.e of Brugmansia arboreashowed to be a competitive antagonist of muscarinic receptors in the isolated intestine, in agreement withthe presence of tropane alkaloids. Moreover, H. connatum and C. pachystachya also produced certainsedation, which could contribute to the antispasmodic effect. The tincture of Hypericum connatum, but notthat of Berberis ruscifolia, reduced the spontaneous locomotion and exploration of mice in the open-fieldtest, at doses of 200 mg leaves/Kg. Results suggest that the three native plants exhibited an importantantispasmodic effect mainly due to non-competitive antagonism of the agonist and of Ca2+-influx to smoothmuscle.Fil: Matera, Soledad Inés. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; ArgentinaFil: Bruno, F.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; ArgentinaFil: Perez, Vanina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; ArgentinaFil: Querini, C.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; ArgentinaFil: Ragone, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; ArgentinaFil: Consolini, Alicia Elvira. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; ArgentinaFil: Bayley, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; ArgentinaSocietà Italo-Latinoamericana di Etnomedicina2016-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/55929Matera, Soledad Inés; Bruno, F.; Perez, Vanina Andrea; Querini, C.; Ragone, María Inés; et al.; Intestinal antispasmodic effects of three argentinian plants: Hypericum connatum, Berberis ruscifolia and Cecropia pachystachya: mechanisms of action and comparison with the effects of Brugmansia arborea; Società Italo-Latinoamericana di Etnomedicina; Pharmacology Online; 2; 8-2016; 91-991827-8620CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://pharmacologyonline.silae.it/front/archives_2016_2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:04:37Zoai:ri.conicet.gov.ar:11336/55929instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:04:38.192CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Intestinal antispasmodic effects of three argentinian plants: Hypericum connatum, Berberis ruscifolia and Cecropia pachystachya: mechanisms of action and comparison with the effects of Brugmansia arborea |
title |
Intestinal antispasmodic effects of three argentinian plants: Hypericum connatum, Berberis ruscifolia and Cecropia pachystachya: mechanisms of action and comparison with the effects of Brugmansia arborea |
spellingShingle |
Intestinal antispasmodic effects of three argentinian plants: Hypericum connatum, Berberis ruscifolia and Cecropia pachystachya: mechanisms of action and comparison with the effects of Brugmansia arborea Matera, Soledad Inés Hypericum Connatum Berberis Ruscifolia Cecropia Pachystachya Brugmansia Arborea Antispasmodic Ca2+-Blockade Sedative |
title_short |
Intestinal antispasmodic effects of three argentinian plants: Hypericum connatum, Berberis ruscifolia and Cecropia pachystachya: mechanisms of action and comparison with the effects of Brugmansia arborea |
title_full |
Intestinal antispasmodic effects of three argentinian plants: Hypericum connatum, Berberis ruscifolia and Cecropia pachystachya: mechanisms of action and comparison with the effects of Brugmansia arborea |
title_fullStr |
Intestinal antispasmodic effects of three argentinian plants: Hypericum connatum, Berberis ruscifolia and Cecropia pachystachya: mechanisms of action and comparison with the effects of Brugmansia arborea |
title_full_unstemmed |
Intestinal antispasmodic effects of three argentinian plants: Hypericum connatum, Berberis ruscifolia and Cecropia pachystachya: mechanisms of action and comparison with the effects of Brugmansia arborea |
title_sort |
Intestinal antispasmodic effects of three argentinian plants: Hypericum connatum, Berberis ruscifolia and Cecropia pachystachya: mechanisms of action and comparison with the effects of Brugmansia arborea |
dc.creator.none.fl_str_mv |
Matera, Soledad Inés Bruno, F. Perez, Vanina Andrea Querini, C. Ragone, María Inés Consolini, Alicia Elvira Bayley, Matias |
author |
Matera, Soledad Inés |
author_facet |
Matera, Soledad Inés Bruno, F. Perez, Vanina Andrea Querini, C. Ragone, María Inés Consolini, Alicia Elvira Bayley, Matias |
author_role |
author |
author2 |
Bruno, F. Perez, Vanina Andrea Querini, C. Ragone, María Inés Consolini, Alicia Elvira Bayley, Matias |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Hypericum Connatum Berberis Ruscifolia Cecropia Pachystachya Brugmansia Arborea Antispasmodic Ca2+-Blockade Sedative |
topic |
Hypericum Connatum Berberis Ruscifolia Cecropia Pachystachya Brugmansia Arborea Antispasmodic Ca2+-Blockade Sedative |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Some medicinal plants are used in Argentina to reduce gastrointestinal symptoms as antispasmodics, but notall these effects were validated. This work studied the effects of three native plants as the ethanolic tinctures(T) of Hypericum connatum and Berberis ruscifolia, as well as the acqueous crude extracts (A.c.e) of Cecropiapachystachya, and compared the mechanisms with those of the peruvian plant Brugmansia arborea inisolated rat intestines. The first three plants demonstrated to have antispasmodic effect mainly due to thenon-competitive blockade of the agonist-induced contraction and calcium influx to the smooth muscle,which was associated to the presence of flavonoids. H. connatum also induced the release of prostaglandinswhich cause intestinal contraction, and NO which cause peristalsis, in a way that the blockade of bothmechanisms potentiated the relaxant effect of the T-H.c. In contrast, the A.c.e of Brugmansia arboreashowed to be a competitive antagonist of muscarinic receptors in the isolated intestine, in agreement withthe presence of tropane alkaloids. Moreover, H. connatum and C. pachystachya also produced certainsedation, which could contribute to the antispasmodic effect. The tincture of Hypericum connatum, but notthat of Berberis ruscifolia, reduced the spontaneous locomotion and exploration of mice in the open-fieldtest, at doses of 200 mg leaves/Kg. Results suggest that the three native plants exhibited an importantantispasmodic effect mainly due to non-competitive antagonism of the agonist and of Ca2+-influx to smoothmuscle. Fil: Matera, Soledad Inés. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina Fil: Bruno, F.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina Fil: Perez, Vanina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina Fil: Querini, C.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina Fil: Ragone, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina Fil: Consolini, Alicia Elvira. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina Fil: Bayley, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina |
description |
Some medicinal plants are used in Argentina to reduce gastrointestinal symptoms as antispasmodics, but notall these effects were validated. This work studied the effects of three native plants as the ethanolic tinctures(T) of Hypericum connatum and Berberis ruscifolia, as well as the acqueous crude extracts (A.c.e) of Cecropiapachystachya, and compared the mechanisms with those of the peruvian plant Brugmansia arborea inisolated rat intestines. The first three plants demonstrated to have antispasmodic effect mainly due to thenon-competitive blockade of the agonist-induced contraction and calcium influx to the smooth muscle,which was associated to the presence of flavonoids. H. connatum also induced the release of prostaglandinswhich cause intestinal contraction, and NO which cause peristalsis, in a way that the blockade of bothmechanisms potentiated the relaxant effect of the T-H.c. In contrast, the A.c.e of Brugmansia arboreashowed to be a competitive antagonist of muscarinic receptors in the isolated intestine, in agreement withthe presence of tropane alkaloids. Moreover, H. connatum and C. pachystachya also produced certainsedation, which could contribute to the antispasmodic effect. The tincture of Hypericum connatum, but notthat of Berberis ruscifolia, reduced the spontaneous locomotion and exploration of mice in the open-fieldtest, at doses of 200 mg leaves/Kg. Results suggest that the three native plants exhibited an importantantispasmodic effect mainly due to non-competitive antagonism of the agonist and of Ca2+-influx to smoothmuscle. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/55929 Matera, Soledad Inés; Bruno, F.; Perez, Vanina Andrea; Querini, C.; Ragone, María Inés; et al.; Intestinal antispasmodic effects of three argentinian plants: Hypericum connatum, Berberis ruscifolia and Cecropia pachystachya: mechanisms of action and comparison with the effects of Brugmansia arborea; Società Italo-Latinoamericana di Etnomedicina; Pharmacology Online; 2; 8-2016; 91-99 1827-8620 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/55929 |
identifier_str_mv |
Matera, Soledad Inés; Bruno, F.; Perez, Vanina Andrea; Querini, C.; Ragone, María Inés; et al.; Intestinal antispasmodic effects of three argentinian plants: Hypericum connatum, Berberis ruscifolia and Cecropia pachystachya: mechanisms of action and comparison with the effects of Brugmansia arborea; Società Italo-Latinoamericana di Etnomedicina; Pharmacology Online; 2; 8-2016; 91-99 1827-8620 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://pharmacologyonline.silae.it/front/archives_2016_2 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Società Italo-Latinoamericana di Etnomedicina |
publisher.none.fl_str_mv |
Società Italo-Latinoamericana di Etnomedicina |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269866843701248 |
score |
13.13397 |