Intestinal antispasmodic effects of three argentinian plants: Hypericum connatum, Berberis ruscifolia and Cecropia pachystachya: mechanisms of action and comparison with the effect...

Autores
Matera, Soledad Inés; Bruno, F.; Perez, Vanina Andrea; Querini, C.; Ragone, María Inés; Consolini, Alicia Elvira; Bayley, Matias
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Some medicinal plants are used in Argentina to reduce gastrointestinal symptoms as antispasmodics, but notall these effects were validated. This work studied the effects of three native plants as the ethanolic tinctures(T) of Hypericum connatum and Berberis ruscifolia, as well as the acqueous crude extracts (A.c.e) of Cecropiapachystachya, and compared the mechanisms with those of the peruvian plant Brugmansia arborea inisolated rat intestines. The first three plants demonstrated to have antispasmodic effect mainly due to thenon-competitive blockade of the agonist-induced contraction and calcium influx to the smooth muscle,which was associated to the presence of flavonoids. H. connatum also induced the release of prostaglandinswhich cause intestinal contraction, and NO which cause peristalsis, in a way that the blockade of bothmechanisms potentiated the relaxant effect of the T-H.c. In contrast, the A.c.e of Brugmansia arboreashowed to be a competitive antagonist of muscarinic receptors in the isolated intestine, in agreement withthe presence of tropane alkaloids. Moreover, H. connatum and C. pachystachya also produced certainsedation, which could contribute to the antispasmodic effect. The tincture of Hypericum connatum, but notthat of Berberis ruscifolia, reduced the spontaneous locomotion and exploration of mice in the open-fieldtest, at doses of 200 mg leaves/Kg. Results suggest that the three native plants exhibited an importantantispasmodic effect mainly due to non-competitive antagonism of the agonist and of Ca2+-influx to smoothmuscle.
Fil: Matera, Soledad Inés. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
Fil: Bruno, F.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
Fil: Perez, Vanina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
Fil: Querini, C.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
Fil: Ragone, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
Fil: Consolini, Alicia Elvira. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
Fil: Bayley, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
Materia
Hypericum Connatum
Berberis Ruscifolia
Cecropia Pachystachya
Brugmansia Arborea
Antispasmodic
Ca2+-Blockade
Sedative
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/55929
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/55929
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Intestinal antispasmodic effects of three argentinian plants: Hypericum connatum, Berberis ruscifolia and Cecropia pachystachya: mechanisms of action and comparison with the effects of Brugmansia arboreaMatera, Soledad InésBruno, F.Perez, Vanina AndreaQuerini, C.Ragone, María InésConsolini, Alicia ElviraBayley, MatiasHypericum ConnatumBerberis RuscifoliaCecropia PachystachyaBrugmansia ArboreaAntispasmodicCa2+-BlockadeSedativehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Some medicinal plants are used in Argentina to reduce gastrointestinal symptoms as antispasmodics, but notall these effects were validated. This work studied the effects of three native plants as the ethanolic tinctures(T) of Hypericum connatum and Berberis ruscifolia, as well as the acqueous crude extracts (A.c.e) of Cecropiapachystachya, and compared the mechanisms with those of the peruvian plant Brugmansia arborea inisolated rat intestines. The first three plants demonstrated to have antispasmodic effect mainly due to thenon-competitive blockade of the agonist-induced contraction and calcium influx to the smooth muscle,which was associated to the presence of flavonoids. H. connatum also induced the release of prostaglandinswhich cause intestinal contraction, and NO which cause peristalsis, in a way that the blockade of bothmechanisms potentiated the relaxant effect of the T-H.c. In contrast, the A.c.e of Brugmansia arboreashowed to be a competitive antagonist of muscarinic receptors in the isolated intestine, in agreement withthe presence of tropane alkaloids. Moreover, H. connatum and C. pachystachya also produced certainsedation, which could contribute to the antispasmodic effect. The tincture of Hypericum connatum, but notthat of Berberis ruscifolia, reduced the spontaneous locomotion and exploration of mice in the open-fieldtest, at doses of 200 mg leaves/Kg. Results suggest that the three native plants exhibited an importantantispasmodic effect mainly due to non-competitive antagonism of the agonist and of Ca2+-influx to smoothmuscle.Fil: Matera, Soledad Inés. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; ArgentinaFil: Bruno, F.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; ArgentinaFil: Perez, Vanina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; ArgentinaFil: Querini, C.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; ArgentinaFil: Ragone, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; ArgentinaFil: Consolini, Alicia Elvira. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; ArgentinaFil: Bayley, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; ArgentinaSocietà Italo-Latinoamericana di Etnomedicina2016-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/55929Matera, Soledad Inés; Bruno, F.; Perez, Vanina Andrea; Querini, C.; Ragone, María Inés; et al.; Intestinal antispasmodic effects of three argentinian plants: Hypericum connatum, Berberis ruscifolia and Cecropia pachystachya: mechanisms of action and comparison with the effects of Brugmansia arborea; Società Italo-Latinoamericana di Etnomedicina; Pharmacology Online; 2; 8-2016; 91-991827-8620CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://pharmacologyonline.silae.it/front/archives_2016_2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:24:03Zoai:ri.conicet.gov.ar:11336/55929instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:24:04.164CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Intestinal antispasmodic effects of three argentinian plants: Hypericum connatum, Berberis ruscifolia and Cecropia pachystachya: mechanisms of action and comparison with the effects of Brugmansia arborea
title Intestinal antispasmodic effects of three argentinian plants: Hypericum connatum, Berberis ruscifolia and Cecropia pachystachya: mechanisms of action and comparison with the effects of Brugmansia arborea
spellingShingle Intestinal antispasmodic effects of three argentinian plants: Hypericum connatum, Berberis ruscifolia and Cecropia pachystachya: mechanisms of action and comparison with the effects of Brugmansia arborea
Matera, Soledad Inés
Hypericum Connatum
Berberis Ruscifolia
Cecropia Pachystachya
Brugmansia Arborea
Antispasmodic
Ca2+-Blockade
Sedative
title_short Intestinal antispasmodic effects of three argentinian plants: Hypericum connatum, Berberis ruscifolia and Cecropia pachystachya: mechanisms of action and comparison with the effects of Brugmansia arborea
title_full Intestinal antispasmodic effects of three argentinian plants: Hypericum connatum, Berberis ruscifolia and Cecropia pachystachya: mechanisms of action and comparison with the effects of Brugmansia arborea
title_fullStr Intestinal antispasmodic effects of three argentinian plants: Hypericum connatum, Berberis ruscifolia and Cecropia pachystachya: mechanisms of action and comparison with the effects of Brugmansia arborea
title_full_unstemmed Intestinal antispasmodic effects of three argentinian plants: Hypericum connatum, Berberis ruscifolia and Cecropia pachystachya: mechanisms of action and comparison with the effects of Brugmansia arborea
title_sort Intestinal antispasmodic effects of three argentinian plants: Hypericum connatum, Berberis ruscifolia and Cecropia pachystachya: mechanisms of action and comparison with the effects of Brugmansia arborea
dc.creator.none.fl_str_mv Matera, Soledad Inés
Bruno, F.
Perez, Vanina Andrea
Querini, C.
Ragone, María Inés
Consolini, Alicia Elvira
Bayley, Matias
author Matera, Soledad Inés
author_facet Matera, Soledad Inés
Bruno, F.
Perez, Vanina Andrea
Querini, C.
Ragone, María Inés
Consolini, Alicia Elvira
Bayley, Matias
author_role author
author2 Bruno, F.
Perez, Vanina Andrea
Querini, C.
Ragone, María Inés
Consolini, Alicia Elvira
Bayley, Matias
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Hypericum Connatum
Berberis Ruscifolia
Cecropia Pachystachya
Brugmansia Arborea
Antispasmodic
Ca2+-Blockade
Sedative
topic Hypericum Connatum
Berberis Ruscifolia
Cecropia Pachystachya
Brugmansia Arborea
Antispasmodic
Ca2+-Blockade
Sedative
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Some medicinal plants are used in Argentina to reduce gastrointestinal symptoms as antispasmodics, but notall these effects were validated. This work studied the effects of three native plants as the ethanolic tinctures(T) of Hypericum connatum and Berberis ruscifolia, as well as the acqueous crude extracts (A.c.e) of Cecropiapachystachya, and compared the mechanisms with those of the peruvian plant Brugmansia arborea inisolated rat intestines. The first three plants demonstrated to have antispasmodic effect mainly due to thenon-competitive blockade of the agonist-induced contraction and calcium influx to the smooth muscle,which was associated to the presence of flavonoids. H. connatum also induced the release of prostaglandinswhich cause intestinal contraction, and NO which cause peristalsis, in a way that the blockade of bothmechanisms potentiated the relaxant effect of the T-H.c. In contrast, the A.c.e of Brugmansia arboreashowed to be a competitive antagonist of muscarinic receptors in the isolated intestine, in agreement withthe presence of tropane alkaloids. Moreover, H. connatum and C. pachystachya also produced certainsedation, which could contribute to the antispasmodic effect. The tincture of Hypericum connatum, but notthat of Berberis ruscifolia, reduced the spontaneous locomotion and exploration of mice in the open-fieldtest, at doses of 200 mg leaves/Kg. Results suggest that the three native plants exhibited an importantantispasmodic effect mainly due to non-competitive antagonism of the agonist and of Ca2+-influx to smoothmuscle.
Fil: Matera, Soledad Inés. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
Fil: Bruno, F.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
Fil: Perez, Vanina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
Fil: Querini, C.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
Fil: Ragone, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
Fil: Consolini, Alicia Elvira. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
Fil: Bayley, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
description Some medicinal plants are used in Argentina to reduce gastrointestinal symptoms as antispasmodics, but notall these effects were validated. This work studied the effects of three native plants as the ethanolic tinctures(T) of Hypericum connatum and Berberis ruscifolia, as well as the acqueous crude extracts (A.c.e) of Cecropiapachystachya, and compared the mechanisms with those of the peruvian plant Brugmansia arborea inisolated rat intestines. The first three plants demonstrated to have antispasmodic effect mainly due to thenon-competitive blockade of the agonist-induced contraction and calcium influx to the smooth muscle,which was associated to the presence of flavonoids. H. connatum also induced the release of prostaglandinswhich cause intestinal contraction, and NO which cause peristalsis, in a way that the blockade of bothmechanisms potentiated the relaxant effect of the T-H.c. In contrast, the A.c.e of Brugmansia arboreashowed to be a competitive antagonist of muscarinic receptors in the isolated intestine, in agreement withthe presence of tropane alkaloids. Moreover, H. connatum and C. pachystachya also produced certainsedation, which could contribute to the antispasmodic effect. The tincture of Hypericum connatum, but notthat of Berberis ruscifolia, reduced the spontaneous locomotion and exploration of mice in the open-fieldtest, at doses of 200 mg leaves/Kg. Results suggest that the three native plants exhibited an importantantispasmodic effect mainly due to non-competitive antagonism of the agonist and of Ca2+-influx to smoothmuscle.
publishDate 2016
dc.date.none.fl_str_mv 2016-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/55929
Matera, Soledad Inés; Bruno, F.; Perez, Vanina Andrea; Querini, C.; Ragone, María Inés; et al.; Intestinal antispasmodic effects of three argentinian plants: Hypericum connatum, Berberis ruscifolia and Cecropia pachystachya: mechanisms of action and comparison with the effects of Brugmansia arborea; Società Italo-Latinoamericana di Etnomedicina; Pharmacology Online; 2; 8-2016; 91-99
1827-8620
CONICET Digital
CONICET
url http://hdl.handle.net/11336/55929
identifier_str_mv Matera, Soledad Inés; Bruno, F.; Perez, Vanina Andrea; Querini, C.; Ragone, María Inés; et al.; Intestinal antispasmodic effects of three argentinian plants: Hypericum connatum, Berberis ruscifolia and Cecropia pachystachya: mechanisms of action and comparison with the effects of Brugmansia arborea; Società Italo-Latinoamericana di Etnomedicina; Pharmacology Online; 2; 8-2016; 91-99
1827-8620
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://pharmacologyonline.silae.it/front/archives_2016_2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Società Italo-Latinoamericana di Etnomedicina
publisher.none.fl_str_mv Società Italo-Latinoamericana di Etnomedicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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