Sedative and antispasmodic effects of Stevia rebaudiana and noncompetitive inhibition of intestinal contractility by stevioside
- Autores
- Matera, Soledad Inés; Piersante, María Victoria; Ragone, María Inés; Consolini, Alicia Elvira
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Stevia rebaudiana (Asteraceae) is a shrub native from Paraguay whose leaves are used as sweetener due to the presence of stevioside and others. It has also hypoglycemic, hypotensor and diuretic effects. This work studied the effects of both, an aqueous crude extract of Stevia rebaudiana (A.c.e) and the stevioside (Stv) in isolated rat thin intestine, and the effects of A.c.e in mice on the open-field-test. The A.c.e of Stevia rebaudiana reduced the spontaneous locomotion and exploration of mice, at doses between 40 to 975 mg lyophilized/ Kg. In the isolated ileons and duodenums, the A.c.e induced a synergism of the acetylcholine doseresponse curve (ACh-DRC) at 0.1-0.3 mg lyophilized/mL and a non-competitive inhibition at higher concentrations (IC50: 1.22±0.16 mg lyoph/ml, n=6). Stv also non-competitively inhibited the ACh-DRC (IC50: 0.84 ± 0.22 mg/ml). Both, A.c.e and Stv inhibited the Ca2+-DRC in 40 mM K+-media in non-competitive way with IC50 of 1.01 ± 0.13 and 1.73 ± 0.47 mg/mL, respectively. Results suggest that Stevia extract exhibited sedative effect and important antispasmodic effect due to non-competitive antagonism of acetylcholine and Ca2+-influx to smooth muscle.
Fil: Matera, Soledad Inés. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Piersante, María Victoria. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina
Fil: Ragone, María Inés. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Consolini, Alicia Elvira. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina - Materia
-
STEVIA REBAUDIANA
STEVIOSIDE
SPASMODIC
SEDATIVE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/197630
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Sedative and antispasmodic effects of Stevia rebaudiana and noncompetitive inhibition of intestinal contractility by steviosideMatera, Soledad InésPiersante, María VictoriaRagone, María InésConsolini, Alicia ElviraSTEVIA REBAUDIANASTEVIOSIDESPASMODICSEDATIVEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Stevia rebaudiana (Asteraceae) is a shrub native from Paraguay whose leaves are used as sweetener due to the presence of stevioside and others. It has also hypoglycemic, hypotensor and diuretic effects. This work studied the effects of both, an aqueous crude extract of Stevia rebaudiana (A.c.e) and the stevioside (Stv) in isolated rat thin intestine, and the effects of A.c.e in mice on the open-field-test. The A.c.e of Stevia rebaudiana reduced the spontaneous locomotion and exploration of mice, at doses between 40 to 975 mg lyophilized/ Kg. In the isolated ileons and duodenums, the A.c.e induced a synergism of the acetylcholine doseresponse curve (ACh-DRC) at 0.1-0.3 mg lyophilized/mL and a non-competitive inhibition at higher concentrations (IC50: 1.22±0.16 mg lyoph/ml, n=6). Stv also non-competitively inhibited the ACh-DRC (IC50: 0.84 ± 0.22 mg/ml). Both, A.c.e and Stv inhibited the Ca2+-DRC in 40 mM K+-media in non-competitive way with IC50 of 1.01 ± 0.13 and 1.73 ± 0.47 mg/mL, respectively. Results suggest that Stevia extract exhibited sedative effect and important antispasmodic effect due to non-competitive antagonism of acetylcholine and Ca2+-influx to smooth muscle.Fil: Matera, Soledad Inés. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Piersante, María Victoria. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; ArgentinaFil: Ragone, María Inés. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Consolini, Alicia Elvira. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; ArgentinaSocietà Italo-Latinoamericana di Etnomedicina2012-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/197630Matera, Soledad Inés; Piersante, María Victoria; Ragone, María Inés; Consolini, Alicia Elvira; Sedative and antispasmodic effects of Stevia rebaudiana and noncompetitive inhibition of intestinal contractility by stevioside; Società Italo-Latinoamericana di Etnomedicina; Pharmacologyonline; 1; 4-2012; 1-81827-8620CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pharmacologyonline.silae.it/front/specialissues_2012_1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:01Zoai:ri.conicet.gov.ar:11336/197630instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:01.367CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Sedative and antispasmodic effects of Stevia rebaudiana and noncompetitive inhibition of intestinal contractility by stevioside |
title |
Sedative and antispasmodic effects of Stevia rebaudiana and noncompetitive inhibition of intestinal contractility by stevioside |
spellingShingle |
Sedative and antispasmodic effects of Stevia rebaudiana and noncompetitive inhibition of intestinal contractility by stevioside Matera, Soledad Inés STEVIA REBAUDIANA STEVIOSIDE SPASMODIC SEDATIVE |
title_short |
Sedative and antispasmodic effects of Stevia rebaudiana and noncompetitive inhibition of intestinal contractility by stevioside |
title_full |
Sedative and antispasmodic effects of Stevia rebaudiana and noncompetitive inhibition of intestinal contractility by stevioside |
title_fullStr |
Sedative and antispasmodic effects of Stevia rebaudiana and noncompetitive inhibition of intestinal contractility by stevioside |
title_full_unstemmed |
Sedative and antispasmodic effects of Stevia rebaudiana and noncompetitive inhibition of intestinal contractility by stevioside |
title_sort |
Sedative and antispasmodic effects of Stevia rebaudiana and noncompetitive inhibition of intestinal contractility by stevioside |
dc.creator.none.fl_str_mv |
Matera, Soledad Inés Piersante, María Victoria Ragone, María Inés Consolini, Alicia Elvira |
author |
Matera, Soledad Inés |
author_facet |
Matera, Soledad Inés Piersante, María Victoria Ragone, María Inés Consolini, Alicia Elvira |
author_role |
author |
author2 |
Piersante, María Victoria Ragone, María Inés Consolini, Alicia Elvira |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
STEVIA REBAUDIANA STEVIOSIDE SPASMODIC SEDATIVE |
topic |
STEVIA REBAUDIANA STEVIOSIDE SPASMODIC SEDATIVE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Stevia rebaudiana (Asteraceae) is a shrub native from Paraguay whose leaves are used as sweetener due to the presence of stevioside and others. It has also hypoglycemic, hypotensor and diuretic effects. This work studied the effects of both, an aqueous crude extract of Stevia rebaudiana (A.c.e) and the stevioside (Stv) in isolated rat thin intestine, and the effects of A.c.e in mice on the open-field-test. The A.c.e of Stevia rebaudiana reduced the spontaneous locomotion and exploration of mice, at doses between 40 to 975 mg lyophilized/ Kg. In the isolated ileons and duodenums, the A.c.e induced a synergism of the acetylcholine doseresponse curve (ACh-DRC) at 0.1-0.3 mg lyophilized/mL and a non-competitive inhibition at higher concentrations (IC50: 1.22±0.16 mg lyoph/ml, n=6). Stv also non-competitively inhibited the ACh-DRC (IC50: 0.84 ± 0.22 mg/ml). Both, A.c.e and Stv inhibited the Ca2+-DRC in 40 mM K+-media in non-competitive way with IC50 of 1.01 ± 0.13 and 1.73 ± 0.47 mg/mL, respectively. Results suggest that Stevia extract exhibited sedative effect and important antispasmodic effect due to non-competitive antagonism of acetylcholine and Ca2+-influx to smooth muscle. Fil: Matera, Soledad Inés. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina Fil: Piersante, María Victoria. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina Fil: Ragone, María Inés. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina Fil: Consolini, Alicia Elvira. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina |
description |
Stevia rebaudiana (Asteraceae) is a shrub native from Paraguay whose leaves are used as sweetener due to the presence of stevioside and others. It has also hypoglycemic, hypotensor and diuretic effects. This work studied the effects of both, an aqueous crude extract of Stevia rebaudiana (A.c.e) and the stevioside (Stv) in isolated rat thin intestine, and the effects of A.c.e in mice on the open-field-test. The A.c.e of Stevia rebaudiana reduced the spontaneous locomotion and exploration of mice, at doses between 40 to 975 mg lyophilized/ Kg. In the isolated ileons and duodenums, the A.c.e induced a synergism of the acetylcholine doseresponse curve (ACh-DRC) at 0.1-0.3 mg lyophilized/mL and a non-competitive inhibition at higher concentrations (IC50: 1.22±0.16 mg lyoph/ml, n=6). Stv also non-competitively inhibited the ACh-DRC (IC50: 0.84 ± 0.22 mg/ml). Both, A.c.e and Stv inhibited the Ca2+-DRC in 40 mM K+-media in non-competitive way with IC50 of 1.01 ± 0.13 and 1.73 ± 0.47 mg/mL, respectively. Results suggest that Stevia extract exhibited sedative effect and important antispasmodic effect due to non-competitive antagonism of acetylcholine and Ca2+-influx to smooth muscle. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/197630 Matera, Soledad Inés; Piersante, María Victoria; Ragone, María Inés; Consolini, Alicia Elvira; Sedative and antispasmodic effects of Stevia rebaudiana and noncompetitive inhibition of intestinal contractility by stevioside; Società Italo-Latinoamericana di Etnomedicina; Pharmacologyonline; 1; 4-2012; 1-8 1827-8620 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/197630 |
identifier_str_mv |
Matera, Soledad Inés; Piersante, María Victoria; Ragone, María Inés; Consolini, Alicia Elvira; Sedative and antispasmodic effects of Stevia rebaudiana and noncompetitive inhibition of intestinal contractility by stevioside; Società Italo-Latinoamericana di Etnomedicina; Pharmacologyonline; 1; 4-2012; 1-8 1827-8620 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://pharmacologyonline.silae.it/front/specialissues_2012_1 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Società Italo-Latinoamericana di Etnomedicina |
publisher.none.fl_str_mv |
Società Italo-Latinoamericana di Etnomedicina |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269615374204928 |
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13.13397 |