Sedative and antispasmodic effects of Stevia rebaudiana and noncompetitive inhibition of intestinal contractility by stevioside

Autores
Matera, Soledad Inés; Piersante, María Victoria; Ragone, María Inés; Consolini, Alicia Elvira
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Stevia rebaudiana (Asteraceae) is a shrub native from Paraguay whose leaves are used as sweetener due to the presence of stevioside and others. It has also hypoglycemic, hypotensor and diuretic effects. This work studied the effects of both, an aqueous crude extract of Stevia rebaudiana (A.c.e) and the stevioside (Stv) in isolated rat thin intestine, and the effects of A.c.e in mice on the open-field-test. The A.c.e of Stevia rebaudiana reduced the spontaneous locomotion and exploration of mice, at doses between 40 to 975 mg lyophilized/ Kg. In the isolated ileons and duodenums, the A.c.e induced a synergism of the acetylcholine doseresponse curve (ACh-DRC) at 0.1-0.3 mg lyophilized/mL and a non-competitive inhibition at higher concentrations (IC50: 1.22±0.16 mg lyoph/ml, n=6). Stv also non-competitively inhibited the ACh-DRC (IC50: 0.84 ± 0.22 mg/ml). Both, A.c.e and Stv inhibited the Ca2+-DRC in 40 mM K+-media in non-competitive way with IC50 of 1.01 ± 0.13 and 1.73 ± 0.47 mg/mL, respectively. Results suggest that Stevia extract exhibited sedative effect and important antispasmodic effect due to non-competitive antagonism of acetylcholine and Ca2+-influx to smooth muscle.
Fil: Matera, Soledad Inés. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Piersante, María Victoria. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina
Fil: Ragone, María Inés. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Consolini, Alicia Elvira. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina
Materia
STEVIA REBAUDIANA
STEVIOSIDE
SPASMODIC
SEDATIVE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/197630
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sedative and antispasmodic effects of Stevia rebaudiana and noncompetitive inhibition of intestinal contractility by steviosideMatera, Soledad InésPiersante, María VictoriaRagone, María InésConsolini, Alicia ElviraSTEVIA REBAUDIANASTEVIOSIDESPASMODICSEDATIVEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Stevia rebaudiana (Asteraceae) is a shrub native from Paraguay whose leaves are used as sweetener due to the presence of stevioside and others. It has also hypoglycemic, hypotensor and diuretic effects. This work studied the effects of both, an aqueous crude extract of Stevia rebaudiana (A.c.e) and the stevioside (Stv) in isolated rat thin intestine, and the effects of A.c.e in mice on the open-field-test. The A.c.e of Stevia rebaudiana reduced the spontaneous locomotion and exploration of mice, at doses between 40 to 975 mg lyophilized/ Kg. In the isolated ileons and duodenums, the A.c.e induced a synergism of the acetylcholine doseresponse curve (ACh-DRC) at 0.1-0.3 mg lyophilized/mL and a non-competitive inhibition at higher concentrations (IC50: 1.22±0.16 mg lyoph/ml, n=6). Stv also non-competitively inhibited the ACh-DRC (IC50: 0.84 ± 0.22 mg/ml). Both, A.c.e and Stv inhibited the Ca2+-DRC in 40 mM K+-media in non-competitive way with IC50 of 1.01 ± 0.13 and 1.73 ± 0.47 mg/mL, respectively. Results suggest that Stevia extract exhibited sedative effect and important antispasmodic effect due to non-competitive antagonism of acetylcholine and Ca2+-influx to smooth muscle.Fil: Matera, Soledad Inés. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Piersante, María Victoria. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; ArgentinaFil: Ragone, María Inés. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Consolini, Alicia Elvira. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; ArgentinaSocietà Italo-Latinoamericana di Etnomedicina2012-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/197630Matera, Soledad Inés; Piersante, María Victoria; Ragone, María Inés; Consolini, Alicia Elvira; Sedative and antispasmodic effects of Stevia rebaudiana and noncompetitive inhibition of intestinal contractility by stevioside; Società Italo-Latinoamericana di Etnomedicina; Pharmacologyonline; 1; 4-2012; 1-81827-8620CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pharmacologyonline.silae.it/front/specialissues_2012_1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:10:50Zoai:ri.conicet.gov.ar:11336/197630instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:10:50.399CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sedative and antispasmodic effects of Stevia rebaudiana and noncompetitive inhibition of intestinal contractility by stevioside
title Sedative and antispasmodic effects of Stevia rebaudiana and noncompetitive inhibition of intestinal contractility by stevioside
spellingShingle Sedative and antispasmodic effects of Stevia rebaudiana and noncompetitive inhibition of intestinal contractility by stevioside
Matera, Soledad Inés
STEVIA REBAUDIANA
STEVIOSIDE
SPASMODIC
SEDATIVE
title_short Sedative and antispasmodic effects of Stevia rebaudiana and noncompetitive inhibition of intestinal contractility by stevioside
title_full Sedative and antispasmodic effects of Stevia rebaudiana and noncompetitive inhibition of intestinal contractility by stevioside
title_fullStr Sedative and antispasmodic effects of Stevia rebaudiana and noncompetitive inhibition of intestinal contractility by stevioside
title_full_unstemmed Sedative and antispasmodic effects of Stevia rebaudiana and noncompetitive inhibition of intestinal contractility by stevioside
title_sort Sedative and antispasmodic effects of Stevia rebaudiana and noncompetitive inhibition of intestinal contractility by stevioside
dc.creator.none.fl_str_mv Matera, Soledad Inés
Piersante, María Victoria
Ragone, María Inés
Consolini, Alicia Elvira
author Matera, Soledad Inés
author_facet Matera, Soledad Inés
Piersante, María Victoria
Ragone, María Inés
Consolini, Alicia Elvira
author_role author
author2 Piersante, María Victoria
Ragone, María Inés
Consolini, Alicia Elvira
author2_role author
author
author
dc.subject.none.fl_str_mv STEVIA REBAUDIANA
STEVIOSIDE
SPASMODIC
SEDATIVE
topic STEVIA REBAUDIANA
STEVIOSIDE
SPASMODIC
SEDATIVE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Stevia rebaudiana (Asteraceae) is a shrub native from Paraguay whose leaves are used as sweetener due to the presence of stevioside and others. It has also hypoglycemic, hypotensor and diuretic effects. This work studied the effects of both, an aqueous crude extract of Stevia rebaudiana (A.c.e) and the stevioside (Stv) in isolated rat thin intestine, and the effects of A.c.e in mice on the open-field-test. The A.c.e of Stevia rebaudiana reduced the spontaneous locomotion and exploration of mice, at doses between 40 to 975 mg lyophilized/ Kg. In the isolated ileons and duodenums, the A.c.e induced a synergism of the acetylcholine doseresponse curve (ACh-DRC) at 0.1-0.3 mg lyophilized/mL and a non-competitive inhibition at higher concentrations (IC50: 1.22±0.16 mg lyoph/ml, n=6). Stv also non-competitively inhibited the ACh-DRC (IC50: 0.84 ± 0.22 mg/ml). Both, A.c.e and Stv inhibited the Ca2+-DRC in 40 mM K+-media in non-competitive way with IC50 of 1.01 ± 0.13 and 1.73 ± 0.47 mg/mL, respectively. Results suggest that Stevia extract exhibited sedative effect and important antispasmodic effect due to non-competitive antagonism of acetylcholine and Ca2+-influx to smooth muscle.
Fil: Matera, Soledad Inés. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Piersante, María Victoria. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina
Fil: Ragone, María Inés. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Consolini, Alicia Elvira. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina
description Stevia rebaudiana (Asteraceae) is a shrub native from Paraguay whose leaves are used as sweetener due to the presence of stevioside and others. It has also hypoglycemic, hypotensor and diuretic effects. This work studied the effects of both, an aqueous crude extract of Stevia rebaudiana (A.c.e) and the stevioside (Stv) in isolated rat thin intestine, and the effects of A.c.e in mice on the open-field-test. The A.c.e of Stevia rebaudiana reduced the spontaneous locomotion and exploration of mice, at doses between 40 to 975 mg lyophilized/ Kg. In the isolated ileons and duodenums, the A.c.e induced a synergism of the acetylcholine doseresponse curve (ACh-DRC) at 0.1-0.3 mg lyophilized/mL and a non-competitive inhibition at higher concentrations (IC50: 1.22±0.16 mg lyoph/ml, n=6). Stv also non-competitively inhibited the ACh-DRC (IC50: 0.84 ± 0.22 mg/ml). Both, A.c.e and Stv inhibited the Ca2+-DRC in 40 mM K+-media in non-competitive way with IC50 of 1.01 ± 0.13 and 1.73 ± 0.47 mg/mL, respectively. Results suggest that Stevia extract exhibited sedative effect and important antispasmodic effect due to non-competitive antagonism of acetylcholine and Ca2+-influx to smooth muscle.
publishDate 2012
dc.date.none.fl_str_mv 2012-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/197630
Matera, Soledad Inés; Piersante, María Victoria; Ragone, María Inés; Consolini, Alicia Elvira; Sedative and antispasmodic effects of Stevia rebaudiana and noncompetitive inhibition of intestinal contractility by stevioside; Società Italo-Latinoamericana di Etnomedicina; Pharmacologyonline; 1; 4-2012; 1-8
1827-8620
CONICET Digital
CONICET
url http://hdl.handle.net/11336/197630
identifier_str_mv Matera, Soledad Inés; Piersante, María Victoria; Ragone, María Inés; Consolini, Alicia Elvira; Sedative and antispasmodic effects of Stevia rebaudiana and noncompetitive inhibition of intestinal contractility by stevioside; Società Italo-Latinoamericana di Etnomedicina; Pharmacologyonline; 1; 4-2012; 1-8
1827-8620
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pharmacologyonline.silae.it/front/specialissues_2012_1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Società Italo-Latinoamericana di Etnomedicina
publisher.none.fl_str_mv Società Italo-Latinoamericana di Etnomedicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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