Intestinal antispasmodic effects of three Argentinian plants: <i>Hypericum connatum</i>, <i>Berberis ruscifolia</i> and <i>Cecropia pachystachya</i>: mechanisms of action and compa...

Autores
Matera, Soledad Inés; Bruno, Fiorella Gianina; Pérez, Vanina Andrea; Querini, C. A.; Ragone, María Inés; Consolini, Alicia Elvira; Bayley Agnoli, Matías
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Some medicinal plants are used in Argentina to reduce gastrointestinal symptoms as antispasmodics, but notall these effects were validated. This work studied the effects of three native plants as the ethanolic tinctures(T) of Hypericum connatum and Berberis ruscifolia, as well as the acqueous crude extracts (A.c.e) of Cecropiapachystachya, and compared the mechanisms with those of the peruvian plant Brugmansia arborea inisolated rat intestines. The first three plants demonstrated to have antispasmodic effect mainly due to thenon-competitive blockade of the agonist-induced contraction and calcium influx to the smooth muscle,which was associated to the presence of flavonoids. H. connatum also induced the release of prostaglandinswhich cause intestinal contraction, and NO which cause peristalsis, in a way that the blockade of bothmechanisms potentiated the relaxant effect of the T-H.c. In contrast, the A.c.e of Brugmansia arboreashowed to be a competitive antagonist of muscarinic receptors in the isolated intestine, in agreement withthe presence of tropane alkaloids. Moreover, H. connatum and C. pachystachya also produced certainsedation, which could contribute to the antispasmodic effect. The tincture of Hypericum connatum, but notthat of Berberis ruscifolia, reduced the spontaneous locomotion and exploration of mice in the open-fieldtest, at doses of 200 mg leaves/Kg. Results suggest that the three native plants exhibited an importantantispasmodic effect mainly due to non-competitive antagonism of the agonist and of Ca2+-influx to smoothmuscle.
Facultad de Ciencias Exactas
Materia
Biología
<i>Hypericum connatum</i>
<i>Berberis ruscifolia</i>
<i>Cecropia pachystachya</i>
<i>Brugmansia arborea</i>
Antispasmodic
Ca2+-blockade
Sedative
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/97939

id SEDICI_2e16c4a3e22475455cd9fb71a9e7426a
oai_identifier_str oai:sedici.unlp.edu.ar:10915/97939
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Intestinal antispasmodic effects of three Argentinian plants: <i>Hypericum connatum</i>, <i>Berberis ruscifolia</i> and <i>Cecropia pachystachya</i>: mechanisms of action and comparison with the effects of <i>Brugmansia arborea</i>Matera, Soledad InésBruno, Fiorella GianinaPérez, Vanina AndreaQuerini, C. A.Ragone, María InésConsolini, Alicia ElviraBayley Agnoli, MatíasBiología<i>Hypericum connatum</i><i>Berberis ruscifolia</i><i>Cecropia pachystachya</i><i>Brugmansia arborea</i>AntispasmodicCa2+-blockadeSedativeSome medicinal plants are used in Argentina to reduce gastrointestinal symptoms as antispasmodics, but notall these effects were validated. This work studied the effects of three native plants as the ethanolic tinctures(T) of Hypericum connatum and Berberis ruscifolia, as well as the acqueous crude extracts (A.c.e) of Cecropiapachystachya, and compared the mechanisms with those of the peruvian plant Brugmansia arborea inisolated rat intestines. The first three plants demonstrated to have antispasmodic effect mainly due to thenon-competitive blockade of the agonist-induced contraction and calcium influx to the smooth muscle,which was associated to the presence of flavonoids. H. connatum also induced the release of prostaglandinswhich cause intestinal contraction, and NO which cause peristalsis, in a way that the blockade of bothmechanisms potentiated the relaxant effect of the T-H.c. In contrast, the A.c.e of Brugmansia arboreashowed to be a competitive antagonist of muscarinic receptors in the isolated intestine, in agreement withthe presence of tropane alkaloids. Moreover, H. connatum and C. pachystachya also produced certainsedation, which could contribute to the antispasmodic effect. The tincture of Hypericum connatum, but notthat of Berberis ruscifolia, reduced the spontaneous locomotion and exploration of mice in the open-fieldtest, at doses of 200 mg leaves/Kg. Results suggest that the three native plants exhibited an importantantispasmodic effect mainly due to non-competitive antagonism of the agonist and of Ca2+-influx to smoothmuscle.Facultad de Ciencias Exactas2016-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf91-99http://sedici.unlp.edu.ar/handle/10915/97939enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/55929info:eu-repo/semantics/altIdentifier/url/http://pharmacologyonline.silae.it/files/archives/2016/vol2/PhOL_2016_2_A013_Matera_91_99.pdfinfo:eu-repo/semantics/altIdentifier/issn/1827-8620info:eu-repo/semantics/altIdentifier/hdl/11336/55929info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:52:27Zoai:sedici.unlp.edu.ar:10915/97939Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:52:27.549SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Intestinal antispasmodic effects of three Argentinian plants: <i>Hypericum connatum</i>, <i>Berberis ruscifolia</i> and <i>Cecropia pachystachya</i>: mechanisms of action and comparison with the effects of <i>Brugmansia arborea</i>
title Intestinal antispasmodic effects of three Argentinian plants: <i>Hypericum connatum</i>, <i>Berberis ruscifolia</i> and <i>Cecropia pachystachya</i>: mechanisms of action and comparison with the effects of <i>Brugmansia arborea</i>
spellingShingle Intestinal antispasmodic effects of three Argentinian plants: <i>Hypericum connatum</i>, <i>Berberis ruscifolia</i> and <i>Cecropia pachystachya</i>: mechanisms of action and comparison with the effects of <i>Brugmansia arborea</i>
Matera, Soledad Inés
Biología
<i>Hypericum connatum</i>
<i>Berberis ruscifolia</i>
<i>Cecropia pachystachya</i>
<i>Brugmansia arborea</i>
Antispasmodic
Ca2+-blockade
Sedative
title_short Intestinal antispasmodic effects of three Argentinian plants: <i>Hypericum connatum</i>, <i>Berberis ruscifolia</i> and <i>Cecropia pachystachya</i>: mechanisms of action and comparison with the effects of <i>Brugmansia arborea</i>
title_full Intestinal antispasmodic effects of three Argentinian plants: <i>Hypericum connatum</i>, <i>Berberis ruscifolia</i> and <i>Cecropia pachystachya</i>: mechanisms of action and comparison with the effects of <i>Brugmansia arborea</i>
title_fullStr Intestinal antispasmodic effects of three Argentinian plants: <i>Hypericum connatum</i>, <i>Berberis ruscifolia</i> and <i>Cecropia pachystachya</i>: mechanisms of action and comparison with the effects of <i>Brugmansia arborea</i>
title_full_unstemmed Intestinal antispasmodic effects of three Argentinian plants: <i>Hypericum connatum</i>, <i>Berberis ruscifolia</i> and <i>Cecropia pachystachya</i>: mechanisms of action and comparison with the effects of <i>Brugmansia arborea</i>
title_sort Intestinal antispasmodic effects of three Argentinian plants: <i>Hypericum connatum</i>, <i>Berberis ruscifolia</i> and <i>Cecropia pachystachya</i>: mechanisms of action and comparison with the effects of <i>Brugmansia arborea</i>
dc.creator.none.fl_str_mv Matera, Soledad Inés
Bruno, Fiorella Gianina
Pérez, Vanina Andrea
Querini, C. A.
Ragone, María Inés
Consolini, Alicia Elvira
Bayley Agnoli, Matías
author Matera, Soledad Inés
author_facet Matera, Soledad Inés
Bruno, Fiorella Gianina
Pérez, Vanina Andrea
Querini, C. A.
Ragone, María Inés
Consolini, Alicia Elvira
Bayley Agnoli, Matías
author_role author
author2 Bruno, Fiorella Gianina
Pérez, Vanina Andrea
Querini, C. A.
Ragone, María Inés
Consolini, Alicia Elvira
Bayley Agnoli, Matías
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Biología
<i>Hypericum connatum</i>
<i>Berberis ruscifolia</i>
<i>Cecropia pachystachya</i>
<i>Brugmansia arborea</i>
Antispasmodic
Ca2+-blockade
Sedative
topic Biología
<i>Hypericum connatum</i>
<i>Berberis ruscifolia</i>
<i>Cecropia pachystachya</i>
<i>Brugmansia arborea</i>
Antispasmodic
Ca2+-blockade
Sedative
dc.description.none.fl_txt_mv Some medicinal plants are used in Argentina to reduce gastrointestinal symptoms as antispasmodics, but notall these effects were validated. This work studied the effects of three native plants as the ethanolic tinctures(T) of Hypericum connatum and Berberis ruscifolia, as well as the acqueous crude extracts (A.c.e) of Cecropiapachystachya, and compared the mechanisms with those of the peruvian plant Brugmansia arborea inisolated rat intestines. The first three plants demonstrated to have antispasmodic effect mainly due to thenon-competitive blockade of the agonist-induced contraction and calcium influx to the smooth muscle,which was associated to the presence of flavonoids. H. connatum also induced the release of prostaglandinswhich cause intestinal contraction, and NO which cause peristalsis, in a way that the blockade of bothmechanisms potentiated the relaxant effect of the T-H.c. In contrast, the A.c.e of Brugmansia arboreashowed to be a competitive antagonist of muscarinic receptors in the isolated intestine, in agreement withthe presence of tropane alkaloids. Moreover, H. connatum and C. pachystachya also produced certainsedation, which could contribute to the antispasmodic effect. The tincture of Hypericum connatum, but notthat of Berberis ruscifolia, reduced the spontaneous locomotion and exploration of mice in the open-fieldtest, at doses of 200 mg leaves/Kg. Results suggest that the three native plants exhibited an importantantispasmodic effect mainly due to non-competitive antagonism of the agonist and of Ca2+-influx to smoothmuscle.
Facultad de Ciencias Exactas
description Some medicinal plants are used in Argentina to reduce gastrointestinal symptoms as antispasmodics, but notall these effects were validated. This work studied the effects of three native plants as the ethanolic tinctures(T) of Hypericum connatum and Berberis ruscifolia, as well as the acqueous crude extracts (A.c.e) of Cecropiapachystachya, and compared the mechanisms with those of the peruvian plant Brugmansia arborea inisolated rat intestines. The first three plants demonstrated to have antispasmodic effect mainly due to thenon-competitive blockade of the agonist-induced contraction and calcium influx to the smooth muscle,which was associated to the presence of flavonoids. H. connatum also induced the release of prostaglandinswhich cause intestinal contraction, and NO which cause peristalsis, in a way that the blockade of bothmechanisms potentiated the relaxant effect of the T-H.c. In contrast, the A.c.e of Brugmansia arboreashowed to be a competitive antagonist of muscarinic receptors in the isolated intestine, in agreement withthe presence of tropane alkaloids. Moreover, H. connatum and C. pachystachya also produced certainsedation, which could contribute to the antispasmodic effect. The tincture of Hypericum connatum, but notthat of Berberis ruscifolia, reduced the spontaneous locomotion and exploration of mice in the open-fieldtest, at doses of 200 mg leaves/Kg. Results suggest that the three native plants exhibited an importantantispasmodic effect mainly due to non-competitive antagonism of the agonist and of Ca2+-influx to smoothmuscle.
publishDate 2016
dc.date.none.fl_str_mv 2016-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/97939
url http://sedici.unlp.edu.ar/handle/10915/97939
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/55929
info:eu-repo/semantics/altIdentifier/url/http://pharmacologyonline.silae.it/files/archives/2016/vol2/PhOL_2016_2_A013_Matera_91_99.pdf
info:eu-repo/semantics/altIdentifier/issn/1827-8620
info:eu-repo/semantics/altIdentifier/hdl/11336/55929
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
91-99
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
_version_ 1842260406545940480
score 13.13397