Expression of NRAMP1 and iNOS in Mycobacterium avium subsp. paratuberculosis naturally infected cattle

Autores
Delgado, Fernando Oscar; Estrada Chávez, C.; Romano, Maria Isabel; Paolicchi, F.; Blanco Viera, F.; Capellino, F.; Chavez Gris, G.; Pereira Suárez, A. L.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Paratuberculosis (PTB) is a chronic disease caused by M. avium subsp. paratuberculosis (MAP) that affects several animal species, and some studies have suggested that there may be a relationship between Crohn's disease and PTB. Significant aspects of PTB pathogenesis are not yet completely understood, such as the role of macrophages. Natural resistance-associated macrophage protein 1 (NRAMP1) and the inducible nitric oxide synthase (iNOS) molecules have shown nonspecific effects against several intracellular pathogens residing within macrophages. However, these molecules have been scarcely studied during natural infection with MAP. In this work, changes in NRAMP1 and iNOS expression were surveyed by immunohistochemistry in tissue samples from MAP-infected cattle and healthy controls. Our findings show strong specific immunolabeling against both NRAMP1 and iNOS molecules, throughout granulomatous PTB-compatible lesions in ileum and ileocaecal lymph nodes from paratuberculous cattle compared with uninfected controls, suggesting a relationship between the expression of these molecules and the pathogenesis of PTB disease.
Fil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Estrada Chávez, C.. Universidad Autonoma del Estado de Hidalgo; México. Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco; México
Fil: Romano, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Paolicchi, F.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Blanco Viera, F.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Capellino, F.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Chavez Gris, G.. Universidad Nacional Autónoma de México; México
Fil: Pereira Suárez, A. L.. Universidad de Guadalajara; México. Universidad Autonoma del Estado de Hidalgo; México
Materia
CATTLE
JOHNE'S DISEASE
MAP
NRAMP1
PARATUBERCULOSIS
SLC11A1 AND INOS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/190066
Acceder
id CONICETDig_76156fe3d50a64f42c07fff0ea291f0f
oai_identifier_str oai:ri.conicet.gov.ar:11336/190066
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Expression of NRAMP1 and iNOS in Mycobacterium avium subsp. paratuberculosis naturally infected cattleDelgado, Fernando OscarEstrada Chávez, C.Romano, Maria IsabelPaolicchi, F.Blanco Viera, F.Capellino, F.Chavez Gris, G.Pereira Suárez, A. L.CATTLEJOHNE'S DISEASEMAPNRAMP1PARATUBERCULOSISSLC11A1 AND INOShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Paratuberculosis (PTB) is a chronic disease caused by M. avium subsp. paratuberculosis (MAP) that affects several animal species, and some studies have suggested that there may be a relationship between Crohn's disease and PTB. Significant aspects of PTB pathogenesis are not yet completely understood, such as the role of macrophages. Natural resistance-associated macrophage protein 1 (NRAMP1) and the inducible nitric oxide synthase (iNOS) molecules have shown nonspecific effects against several intracellular pathogens residing within macrophages. However, these molecules have been scarcely studied during natural infection with MAP. In this work, changes in NRAMP1 and iNOS expression were surveyed by immunohistochemistry in tissue samples from MAP-infected cattle and healthy controls. Our findings show strong specific immunolabeling against both NRAMP1 and iNOS molecules, throughout granulomatous PTB-compatible lesions in ileum and ileocaecal lymph nodes from paratuberculous cattle compared with uninfected controls, suggesting a relationship between the expression of these molecules and the pathogenesis of PTB disease.Fil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaFil: Estrada Chávez, C.. Universidad Autonoma del Estado de Hidalgo; México. Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco; MéxicoFil: Romano, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Paolicchi, F.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Blanco Viera, F.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaFil: Capellino, F.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaFil: Chavez Gris, G.. Universidad Nacional Autónoma de México; MéxicoFil: Pereira Suárez, A. L.. Universidad de Guadalajara; México. Universidad Autonoma del Estado de Hidalgo; MéxicoElsevier2010-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/190066Delgado, Fernando Oscar; Estrada Chávez, C.; Romano, Maria Isabel; Paolicchi, F.; Blanco Viera, F.; et al.; Expression of NRAMP1 and iNOS in Mycobacterium avium subsp. paratuberculosis naturally infected cattle; Elsevier; Comparative Immunology, Microbiology and Infectious Diseases; 33; 5; 9-2010; 389-4000147-9571CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0147957109000174info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cimid.2009.03.001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:05:26Zoai:ri.conicet.gov.ar:11336/190066instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:05:27.289CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expression of NRAMP1 and iNOS in Mycobacterium avium subsp. paratuberculosis naturally infected cattle
title Expression of NRAMP1 and iNOS in Mycobacterium avium subsp. paratuberculosis naturally infected cattle
spellingShingle Expression of NRAMP1 and iNOS in Mycobacterium avium subsp. paratuberculosis naturally infected cattle
Delgado, Fernando Oscar
CATTLE
JOHNE'S DISEASE
MAP
NRAMP1
PARATUBERCULOSIS
SLC11A1 AND INOS
title_short Expression of NRAMP1 and iNOS in Mycobacterium avium subsp. paratuberculosis naturally infected cattle
title_full Expression of NRAMP1 and iNOS in Mycobacterium avium subsp. paratuberculosis naturally infected cattle
title_fullStr Expression of NRAMP1 and iNOS in Mycobacterium avium subsp. paratuberculosis naturally infected cattle
title_full_unstemmed Expression of NRAMP1 and iNOS in Mycobacterium avium subsp. paratuberculosis naturally infected cattle
title_sort Expression of NRAMP1 and iNOS in Mycobacterium avium subsp. paratuberculosis naturally infected cattle
dc.creator.none.fl_str_mv Delgado, Fernando Oscar
Estrada Chávez, C.
Romano, Maria Isabel
Paolicchi, F.
Blanco Viera, F.
Capellino, F.
Chavez Gris, G.
Pereira Suárez, A. L.
author Delgado, Fernando Oscar
author_facet Delgado, Fernando Oscar
Estrada Chávez, C.
Romano, Maria Isabel
Paolicchi, F.
Blanco Viera, F.
Capellino, F.
Chavez Gris, G.
Pereira Suárez, A. L.
author_role author
author2 Estrada Chávez, C.
Romano, Maria Isabel
Paolicchi, F.
Blanco Viera, F.
Capellino, F.
Chavez Gris, G.
Pereira Suárez, A. L.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CATTLE
JOHNE'S DISEASE
MAP
NRAMP1
PARATUBERCULOSIS
SLC11A1 AND INOS
topic CATTLE
JOHNE'S DISEASE
MAP
NRAMP1
PARATUBERCULOSIS
SLC11A1 AND INOS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Paratuberculosis (PTB) is a chronic disease caused by M. avium subsp. paratuberculosis (MAP) that affects several animal species, and some studies have suggested that there may be a relationship between Crohn's disease and PTB. Significant aspects of PTB pathogenesis are not yet completely understood, such as the role of macrophages. Natural resistance-associated macrophage protein 1 (NRAMP1) and the inducible nitric oxide synthase (iNOS) molecules have shown nonspecific effects against several intracellular pathogens residing within macrophages. However, these molecules have been scarcely studied during natural infection with MAP. In this work, changes in NRAMP1 and iNOS expression were surveyed by immunohistochemistry in tissue samples from MAP-infected cattle and healthy controls. Our findings show strong specific immunolabeling against both NRAMP1 and iNOS molecules, throughout granulomatous PTB-compatible lesions in ileum and ileocaecal lymph nodes from paratuberculous cattle compared with uninfected controls, suggesting a relationship between the expression of these molecules and the pathogenesis of PTB disease.
Fil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Estrada Chávez, C.. Universidad Autonoma del Estado de Hidalgo; México. Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco; México
Fil: Romano, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Paolicchi, F.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Blanco Viera, F.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Capellino, F.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Chavez Gris, G.. Universidad Nacional Autónoma de México; México
Fil: Pereira Suárez, A. L.. Universidad de Guadalajara; México. Universidad Autonoma del Estado de Hidalgo; México
description Paratuberculosis (PTB) is a chronic disease caused by M. avium subsp. paratuberculosis (MAP) that affects several animal species, and some studies have suggested that there may be a relationship between Crohn's disease and PTB. Significant aspects of PTB pathogenesis are not yet completely understood, such as the role of macrophages. Natural resistance-associated macrophage protein 1 (NRAMP1) and the inducible nitric oxide synthase (iNOS) molecules have shown nonspecific effects against several intracellular pathogens residing within macrophages. However, these molecules have been scarcely studied during natural infection with MAP. In this work, changes in NRAMP1 and iNOS expression were surveyed by immunohistochemistry in tissue samples from MAP-infected cattle and healthy controls. Our findings show strong specific immunolabeling against both NRAMP1 and iNOS molecules, throughout granulomatous PTB-compatible lesions in ileum and ileocaecal lymph nodes from paratuberculous cattle compared with uninfected controls, suggesting a relationship between the expression of these molecules and the pathogenesis of PTB disease.
publishDate 2010
dc.date.none.fl_str_mv 2010-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/190066
Delgado, Fernando Oscar; Estrada Chávez, C.; Romano, Maria Isabel; Paolicchi, F.; Blanco Viera, F.; et al.; Expression of NRAMP1 and iNOS in Mycobacterium avium subsp. paratuberculosis naturally infected cattle; Elsevier; Comparative Immunology, Microbiology and Infectious Diseases; 33; 5; 9-2010; 389-400
0147-9571
CONICET Digital
CONICET
url http://hdl.handle.net/11336/190066
identifier_str_mv Delgado, Fernando Oscar; Estrada Chávez, C.; Romano, Maria Isabel; Paolicchi, F.; Blanco Viera, F.; et al.; Expression of NRAMP1 and iNOS in Mycobacterium avium subsp. paratuberculosis naturally infected cattle; Elsevier; Comparative Immunology, Microbiology and Infectious Diseases; 33; 5; 9-2010; 389-400
0147-9571
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0147957109000174
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cimid.2009.03.001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781333459697664
score 12.982451

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