Use of cross-reactivity immunoassay to orient insulin replacement in diabetic patients with high levels of insulin antibodies

Autores
Cardoso Landaburu, Alejandro Cesar; Pomares, Maria Fernanda; Avalos, Alfredo; Lapertosa, Silvia; Frechtel, Gustavo Daniel; Poskus, Edgardo
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The prevalence and high levels of anti-insulin antibodies (IA) have frequently been associated with brittle diabetes, lipodystrophy in the areas where the insulin is injected and/or poor metabolic control. When this happens the usual criterion adopted is the empirical change of insulin type and/or formulation intending to diminish the IA level and then to decrease the undesirable side-effects. Here, we present a rational two step radiometric method consisting in: A) a first-line radioligand binding assay (RBA) to assess IA in sera of these patients and detecting those with high levels. B) applying a displacement assay (RIA) to determine the in vitro cross-reactivity parameters (affinity constants and selectivity ratios) that quantify the relative degree of interaction between antibodies and alternative insulin analogs. From these results we conclude that conventional criteria for selection of insulin analogs, in terms of pharmacokinetic and pharmacodinamic parameters, should be complemented with an appropriate test to assess affinity parameters when high IA title is demonstrated. • This manuscript introduces a rational method to determine the appropriated insulin replacement when high insulin antibodies levels are present.• This protocol provides instructions and details in mathematical tools and laboratory processes for the analysis of serum samples.• This method proved to be successful in a single case and requires confirmation using a large group of patients.
Fil: Cardoso Landaburu, Alejandro Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina
Fil: Pomares, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Juan Pablo II; Argentina
Fil: Avalos, Alfredo. Hospital José Ramon Vidal; Argentina
Fil: Lapertosa, Silvia. Hospital José Ramon Vidal; Argentina
Fil: Frechtel, Gustavo Daniel. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Poskus, Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina
Materia
BRITTLE DIABETES
DIABETES TYPE 1
INSULIN ANTIBODIES
INSULINS ANALOGUES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/62221
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/62221
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Use of cross-reactivity immunoassay to orient insulin replacement in diabetic patients with high levels of insulin antibodiesCardoso Landaburu, Alejandro CesarPomares, Maria FernandaAvalos, AlfredoLapertosa, SilviaFrechtel, Gustavo DanielPoskus, EdgardoBRITTLE DIABETESDIABETES TYPE 1INSULIN ANTIBODIESINSULINS ANALOGUEShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The prevalence and high levels of anti-insulin antibodies (IA) have frequently been associated with brittle diabetes, lipodystrophy in the areas where the insulin is injected and/or poor metabolic control. When this happens the usual criterion adopted is the empirical change of insulin type and/or formulation intending to diminish the IA level and then to decrease the undesirable side-effects. Here, we present a rational two step radiometric method consisting in: A) a first-line radioligand binding assay (RBA) to assess IA in sera of these patients and detecting those with high levels. B) applying a displacement assay (RIA) to determine the in vitro cross-reactivity parameters (affinity constants and selectivity ratios) that quantify the relative degree of interaction between antibodies and alternative insulin analogs. From these results we conclude that conventional criteria for selection of insulin analogs, in terms of pharmacokinetic and pharmacodinamic parameters, should be complemented with an appropriate test to assess affinity parameters when high IA title is demonstrated. • This manuscript introduces a rational method to determine the appropriated insulin replacement when high insulin antibodies levels are present.• This protocol provides instructions and details in mathematical tools and laboratory processes for the analysis of serum samples.• This method proved to be successful in a single case and requires confirmation using a large group of patients.Fil: Cardoso Landaburu, Alejandro Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Pomares, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Juan Pablo II; ArgentinaFil: Avalos, Alfredo. Hospital José Ramon Vidal; ArgentinaFil: Lapertosa, Silvia. Hospital José Ramon Vidal; ArgentinaFil: Frechtel, Gustavo Daniel. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Poskus, Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaElsevier Science2016-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/62221Cardoso Landaburu, Alejandro Cesar; Pomares, Maria Fernanda; Avalos, Alfredo; Lapertosa, Silvia; Frechtel, Gustavo Daniel; et al.; Use of cross-reactivity immunoassay to orient insulin replacement in diabetic patients with high levels of insulin antibodies; Elsevier Science; MethodsX; 3; 10-2016; 502-5072215-0161CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.mex.2016.08.003info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2215016116300309info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:35:45Zoai:ri.conicet.gov.ar:11336/62221instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:35:45.76CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Use of cross-reactivity immunoassay to orient insulin replacement in diabetic patients with high levels of insulin antibodies
title Use of cross-reactivity immunoassay to orient insulin replacement in diabetic patients with high levels of insulin antibodies
spellingShingle Use of cross-reactivity immunoassay to orient insulin replacement in diabetic patients with high levels of insulin antibodies
Cardoso Landaburu, Alejandro Cesar
BRITTLE DIABETES
DIABETES TYPE 1
INSULIN ANTIBODIES
INSULINS ANALOGUES
title_short Use of cross-reactivity immunoassay to orient insulin replacement in diabetic patients with high levels of insulin antibodies
title_full Use of cross-reactivity immunoassay to orient insulin replacement in diabetic patients with high levels of insulin antibodies
title_fullStr Use of cross-reactivity immunoassay to orient insulin replacement in diabetic patients with high levels of insulin antibodies
title_full_unstemmed Use of cross-reactivity immunoassay to orient insulin replacement in diabetic patients with high levels of insulin antibodies
title_sort Use of cross-reactivity immunoassay to orient insulin replacement in diabetic patients with high levels of insulin antibodies
dc.creator.none.fl_str_mv Cardoso Landaburu, Alejandro Cesar
Pomares, Maria Fernanda
Avalos, Alfredo
Lapertosa, Silvia
Frechtel, Gustavo Daniel
Poskus, Edgardo
author Cardoso Landaburu, Alejandro Cesar
author_facet Cardoso Landaburu, Alejandro Cesar
Pomares, Maria Fernanda
Avalos, Alfredo
Lapertosa, Silvia
Frechtel, Gustavo Daniel
Poskus, Edgardo
author_role author
author2 Pomares, Maria Fernanda
Avalos, Alfredo
Lapertosa, Silvia
Frechtel, Gustavo Daniel
Poskus, Edgardo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BRITTLE DIABETES
DIABETES TYPE 1
INSULIN ANTIBODIES
INSULINS ANALOGUES
topic BRITTLE DIABETES
DIABETES TYPE 1
INSULIN ANTIBODIES
INSULINS ANALOGUES
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The prevalence and high levels of anti-insulin antibodies (IA) have frequently been associated with brittle diabetes, lipodystrophy in the areas where the insulin is injected and/or poor metabolic control. When this happens the usual criterion adopted is the empirical change of insulin type and/or formulation intending to diminish the IA level and then to decrease the undesirable side-effects. Here, we present a rational two step radiometric method consisting in: A) a first-line radioligand binding assay (RBA) to assess IA in sera of these patients and detecting those with high levels. B) applying a displacement assay (RIA) to determine the in vitro cross-reactivity parameters (affinity constants and selectivity ratios) that quantify the relative degree of interaction between antibodies and alternative insulin analogs. From these results we conclude that conventional criteria for selection of insulin analogs, in terms of pharmacokinetic and pharmacodinamic parameters, should be complemented with an appropriate test to assess affinity parameters when high IA title is demonstrated. • This manuscript introduces a rational method to determine the appropriated insulin replacement when high insulin antibodies levels are present.• This protocol provides instructions and details in mathematical tools and laboratory processes for the analysis of serum samples.• This method proved to be successful in a single case and requires confirmation using a large group of patients.
Fil: Cardoso Landaburu, Alejandro Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina
Fil: Pomares, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Juan Pablo II; Argentina
Fil: Avalos, Alfredo. Hospital José Ramon Vidal; Argentina
Fil: Lapertosa, Silvia. Hospital José Ramon Vidal; Argentina
Fil: Frechtel, Gustavo Daniel. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Poskus, Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina
description The prevalence and high levels of anti-insulin antibodies (IA) have frequently been associated with brittle diabetes, lipodystrophy in the areas where the insulin is injected and/or poor metabolic control. When this happens the usual criterion adopted is the empirical change of insulin type and/or formulation intending to diminish the IA level and then to decrease the undesirable side-effects. Here, we present a rational two step radiometric method consisting in: A) a first-line radioligand binding assay (RBA) to assess IA in sera of these patients and detecting those with high levels. B) applying a displacement assay (RIA) to determine the in vitro cross-reactivity parameters (affinity constants and selectivity ratios) that quantify the relative degree of interaction between antibodies and alternative insulin analogs. From these results we conclude that conventional criteria for selection of insulin analogs, in terms of pharmacokinetic and pharmacodinamic parameters, should be complemented with an appropriate test to assess affinity parameters when high IA title is demonstrated. • This manuscript introduces a rational method to determine the appropriated insulin replacement when high insulin antibodies levels are present.• This protocol provides instructions and details in mathematical tools and laboratory processes for the analysis of serum samples.• This method proved to be successful in a single case and requires confirmation using a large group of patients.
publishDate 2016
dc.date.none.fl_str_mv 2016-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/62221
Cardoso Landaburu, Alejandro Cesar; Pomares, Maria Fernanda; Avalos, Alfredo; Lapertosa, Silvia; Frechtel, Gustavo Daniel; et al.; Use of cross-reactivity immunoassay to orient insulin replacement in diabetic patients with high levels of insulin antibodies; Elsevier Science; MethodsX; 3; 10-2016; 502-507
2215-0161
CONICET Digital
CONICET
url http://hdl.handle.net/11336/62221
identifier_str_mv Cardoso Landaburu, Alejandro Cesar; Pomares, Maria Fernanda; Avalos, Alfredo; Lapertosa, Silvia; Frechtel, Gustavo Daniel; et al.; Use of cross-reactivity immunoassay to orient insulin replacement in diabetic patients with high levels of insulin antibodies; Elsevier Science; MethodsX; 3; 10-2016; 502-507
2215-0161
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mex.2016.08.003
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2215016116300309
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082819152936960
score 13.22299

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