Extreme-halophiles: their role in the arsenic biogeochemical cycle

Autores
Rasuk, Maria Cecilia; Ordoñez, Omar Federico; Soria, Mariana Noelia; Farias, Maria Eugenia
Año de publicación
2015
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Biofilms, mats and microbialites dwell under extreme environmental conditions (high salinity, extreme aridity, pH and arsenic concentration) in the Argentinean Puna and the Atacama Desert. Microbial communities inhabiting those ecosystems are poorly known. Arsenic metabolism is proposed to be an ancient mechanism in microbial life. Besides, some bacteria and archaea are not only able to use detoxification processes to grow under high arsenic concentration, but also, some of them are able to exploit arsenic as a bioenergetic substrate in either anaerobic arsenate respiration or chemolithotrophic growth on arsenite. Only four aioAB coding for arsenite oxidase and two arrA coding for arsenate reductase sequences from haloarchaea were previously deposited in the NCBI Database, but have not been reported in the literature. The arrA arsenate reductases are reliable indicators of anaerobic As (V) respiration and catalyze the electron transfer to the As (V) terminal acceptor in dissimilatory arsenatereducing prokaryotes (DARPs). In this work, we are presenting our first steps in the study of the arsenic biogeochemical cycle in these ecosystems. Thus, the aim of this study was to isolate and to study the arsenic metabolism genes of the isolated extreme halophile microorganisms as well as to test the growth in minimal medium using different carbon sources. Mats and microbialites samples were taken from the water’s edge of Laguna Tebenquiche, Laguna Brava (Salar de Atacama, Chile) during December 2012 and from gaylusite crystals (Laguna Diamante) in August 2014. Samples were enriched and plated in WS medium supplemented with arsenic (AsIII 0.5mM and AsV 20mM). Arsenite oxidase (aioB) and Arsenate reductase (arrA) primers specific for haloarchaea were designed using PrimerProspector software. Selected primers were aioB-1190F (5’-GCTCMTSACCGGCAGCGTCG-3’), aioB-1507R (5’-YGATCTCGTCGATGTCGGCG-3’), arrA-417F (5’CCCGAGTTCGAGCCSATCTC-3’) and arrA-614R (5’GCRCAGATCGMGCTGTGGGA-3’). In order to identify the isolates we used Archaea-specific primers for 16S rDNA gene amplification: 344F (5´- ACG GGG YGC AGC AGG CGC GA-3´) and 915R (5´- GTG CTC CCC CGC CAA TTC CT -3´). Fragments of 577 bp, 317pb and 197pb were obtained from 16S rDNA, aioB and arrA genes respectively. Universal primers 27F and 1492R were used to amplify 16S rDNA in bacterial isolates. 25 isolates belonging to Archaea and Bacteria Domain were obtained; they are related to the Phylum Euryarchaeota, Firmicutes and Proteobacteria. AioB and arrA genes were found in most of the isolates and DNA from the samples (mats, microbialites and biofilm). The best carbon source tested was pyruvate and acetate, being pyruvate better in all cases. Promising results were obtained in the search of organisms able to use arsenic in their bioenergetic metabolism. More studies are underway to try to better understand these very interesting systems.
Fil: Rasuk, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Ordoñez, Omar Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Soria, Mariana Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XI Congreso Argentino de Microbiología General
Cordoba
Argentina
Sociedad Argentina de Microbiología General
Materia
HALOFILOS
EXTREMOS
CICLO BIOGEOQUIMICOS
ARSENICO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/189405

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oai_identifier_str oai:ri.conicet.gov.ar:11336/189405
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network_name_str CONICET Digital (CONICET)
spelling Extreme-halophiles: their role in the arsenic biogeochemical cycleRasuk, Maria CeciliaOrdoñez, Omar FedericoSoria, Mariana NoeliaFarias, Maria EugeniaHALOFILOSEXTREMOSCICLO BIOGEOQUIMICOSARSENICOhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Biofilms, mats and microbialites dwell under extreme environmental conditions (high salinity, extreme aridity, pH and arsenic concentration) in the Argentinean Puna and the Atacama Desert. Microbial communities inhabiting those ecosystems are poorly known. Arsenic metabolism is proposed to be an ancient mechanism in microbial life. Besides, some bacteria and archaea are not only able to use detoxification processes to grow under high arsenic concentration, but also, some of them are able to exploit arsenic as a bioenergetic substrate in either anaerobic arsenate respiration or chemolithotrophic growth on arsenite. Only four aioAB coding for arsenite oxidase and two arrA coding for arsenate reductase sequences from haloarchaea were previously deposited in the NCBI Database, but have not been reported in the literature. The arrA arsenate reductases are reliable indicators of anaerobic As (V) respiration and catalyze the electron transfer to the As (V) terminal acceptor in dissimilatory arsenatereducing prokaryotes (DARPs). In this work, we are presenting our first steps in the study of the arsenic biogeochemical cycle in these ecosystems. Thus, the aim of this study was to isolate and to study the arsenic metabolism genes of the isolated extreme halophile microorganisms as well as to test the growth in minimal medium using different carbon sources. Mats and microbialites samples were taken from the water’s edge of Laguna Tebenquiche, Laguna Brava (Salar de Atacama, Chile) during December 2012 and from gaylusite crystals (Laguna Diamante) in August 2014. Samples were enriched and plated in WS medium supplemented with arsenic (AsIII 0.5mM and AsV 20mM). Arsenite oxidase (aioB) and Arsenate reductase (arrA) primers specific for haloarchaea were designed using PrimerProspector software. Selected primers were aioB-1190F (5’-GCTCMTSACCGGCAGCGTCG-3’), aioB-1507R (5’-YGATCTCGTCGATGTCGGCG-3’), arrA-417F (5’CCCGAGTTCGAGCCSATCTC-3’) and arrA-614R (5’GCRCAGATCGMGCTGTGGGA-3’). In order to identify the isolates we used Archaea-specific primers for 16S rDNA gene amplification: 344F (5´- ACG GGG YGC AGC AGG CGC GA-3´) and 915R (5´- GTG CTC CCC CGC CAA TTC CT -3´). Fragments of 577 bp, 317pb and 197pb were obtained from 16S rDNA, aioB and arrA genes respectively. Universal primers 27F and 1492R were used to amplify 16S rDNA in bacterial isolates. 25 isolates belonging to Archaea and Bacteria Domain were obtained; they are related to the Phylum Euryarchaeota, Firmicutes and Proteobacteria. AioB and arrA genes were found in most of the isolates and DNA from the samples (mats, microbialites and biofilm). The best carbon source tested was pyruvate and acetate, being pyruvate better in all cases. Promising results were obtained in the search of organisms able to use arsenic in their bioenergetic metabolism. More studies are underway to try to better understand these very interesting systems.Fil: Rasuk, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Ordoñez, Omar Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Soria, Mariana Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaXI Congreso Argentino de Microbiología GeneralCordobaArgentinaSociedad Argentina de Microbiología GeneralSociedad Argentina de Microbiología General2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/189405Extreme-halophiles: their role in the arsenic biogeochemical cycle; XI Congreso Argentino de Microbiología General; Cordoba; Argentina; 2015; 1-3CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2022/10/Libro-SAMIGE-2015.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:36:07Zoai:ri.conicet.gov.ar:11336/189405instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:36:07.718CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Extreme-halophiles: their role in the arsenic biogeochemical cycle
title Extreme-halophiles: their role in the arsenic biogeochemical cycle
spellingShingle Extreme-halophiles: their role in the arsenic biogeochemical cycle
Rasuk, Maria Cecilia
HALOFILOS
EXTREMOS
CICLO BIOGEOQUIMICOS
ARSENICO
title_short Extreme-halophiles: their role in the arsenic biogeochemical cycle
title_full Extreme-halophiles: their role in the arsenic biogeochemical cycle
title_fullStr Extreme-halophiles: their role in the arsenic biogeochemical cycle
title_full_unstemmed Extreme-halophiles: their role in the arsenic biogeochemical cycle
title_sort Extreme-halophiles: their role in the arsenic biogeochemical cycle
dc.creator.none.fl_str_mv Rasuk, Maria Cecilia
Ordoñez, Omar Federico
Soria, Mariana Noelia
Farias, Maria Eugenia
author Rasuk, Maria Cecilia
author_facet Rasuk, Maria Cecilia
Ordoñez, Omar Federico
Soria, Mariana Noelia
Farias, Maria Eugenia
author_role author
author2 Ordoñez, Omar Federico
Soria, Mariana Noelia
Farias, Maria Eugenia
author2_role author
author
author
dc.subject.none.fl_str_mv HALOFILOS
EXTREMOS
CICLO BIOGEOQUIMICOS
ARSENICO
topic HALOFILOS
EXTREMOS
CICLO BIOGEOQUIMICOS
ARSENICO
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Biofilms, mats and microbialites dwell under extreme environmental conditions (high salinity, extreme aridity, pH and arsenic concentration) in the Argentinean Puna and the Atacama Desert. Microbial communities inhabiting those ecosystems are poorly known. Arsenic metabolism is proposed to be an ancient mechanism in microbial life. Besides, some bacteria and archaea are not only able to use detoxification processes to grow under high arsenic concentration, but also, some of them are able to exploit arsenic as a bioenergetic substrate in either anaerobic arsenate respiration or chemolithotrophic growth on arsenite. Only four aioAB coding for arsenite oxidase and two arrA coding for arsenate reductase sequences from haloarchaea were previously deposited in the NCBI Database, but have not been reported in the literature. The arrA arsenate reductases are reliable indicators of anaerobic As (V) respiration and catalyze the electron transfer to the As (V) terminal acceptor in dissimilatory arsenatereducing prokaryotes (DARPs). In this work, we are presenting our first steps in the study of the arsenic biogeochemical cycle in these ecosystems. Thus, the aim of this study was to isolate and to study the arsenic metabolism genes of the isolated extreme halophile microorganisms as well as to test the growth in minimal medium using different carbon sources. Mats and microbialites samples were taken from the water’s edge of Laguna Tebenquiche, Laguna Brava (Salar de Atacama, Chile) during December 2012 and from gaylusite crystals (Laguna Diamante) in August 2014. Samples were enriched and plated in WS medium supplemented with arsenic (AsIII 0.5mM and AsV 20mM). Arsenite oxidase (aioB) and Arsenate reductase (arrA) primers specific for haloarchaea were designed using PrimerProspector software. Selected primers were aioB-1190F (5’-GCTCMTSACCGGCAGCGTCG-3’), aioB-1507R (5’-YGATCTCGTCGATGTCGGCG-3’), arrA-417F (5’CCCGAGTTCGAGCCSATCTC-3’) and arrA-614R (5’GCRCAGATCGMGCTGTGGGA-3’). In order to identify the isolates we used Archaea-specific primers for 16S rDNA gene amplification: 344F (5´- ACG GGG YGC AGC AGG CGC GA-3´) and 915R (5´- GTG CTC CCC CGC CAA TTC CT -3´). Fragments of 577 bp, 317pb and 197pb were obtained from 16S rDNA, aioB and arrA genes respectively. Universal primers 27F and 1492R were used to amplify 16S rDNA in bacterial isolates. 25 isolates belonging to Archaea and Bacteria Domain were obtained; they are related to the Phylum Euryarchaeota, Firmicutes and Proteobacteria. AioB and arrA genes were found in most of the isolates and DNA from the samples (mats, microbialites and biofilm). The best carbon source tested was pyruvate and acetate, being pyruvate better in all cases. Promising results were obtained in the search of organisms able to use arsenic in their bioenergetic metabolism. More studies are underway to try to better understand these very interesting systems.
Fil: Rasuk, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Ordoñez, Omar Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Soria, Mariana Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XI Congreso Argentino de Microbiología General
Cordoba
Argentina
Sociedad Argentina de Microbiología General
description Biofilms, mats and microbialites dwell under extreme environmental conditions (high salinity, extreme aridity, pH and arsenic concentration) in the Argentinean Puna and the Atacama Desert. Microbial communities inhabiting those ecosystems are poorly known. Arsenic metabolism is proposed to be an ancient mechanism in microbial life. Besides, some bacteria and archaea are not only able to use detoxification processes to grow under high arsenic concentration, but also, some of them are able to exploit arsenic as a bioenergetic substrate in either anaerobic arsenate respiration or chemolithotrophic growth on arsenite. Only four aioAB coding for arsenite oxidase and two arrA coding for arsenate reductase sequences from haloarchaea were previously deposited in the NCBI Database, but have not been reported in the literature. The arrA arsenate reductases are reliable indicators of anaerobic As (V) respiration and catalyze the electron transfer to the As (V) terminal acceptor in dissimilatory arsenatereducing prokaryotes (DARPs). In this work, we are presenting our first steps in the study of the arsenic biogeochemical cycle in these ecosystems. Thus, the aim of this study was to isolate and to study the arsenic metabolism genes of the isolated extreme halophile microorganisms as well as to test the growth in minimal medium using different carbon sources. Mats and microbialites samples were taken from the water’s edge of Laguna Tebenquiche, Laguna Brava (Salar de Atacama, Chile) during December 2012 and from gaylusite crystals (Laguna Diamante) in August 2014. Samples were enriched and plated in WS medium supplemented with arsenic (AsIII 0.5mM and AsV 20mM). Arsenite oxidase (aioB) and Arsenate reductase (arrA) primers specific for haloarchaea were designed using PrimerProspector software. Selected primers were aioB-1190F (5’-GCTCMTSACCGGCAGCGTCG-3’), aioB-1507R (5’-YGATCTCGTCGATGTCGGCG-3’), arrA-417F (5’CCCGAGTTCGAGCCSATCTC-3’) and arrA-614R (5’GCRCAGATCGMGCTGTGGGA-3’). In order to identify the isolates we used Archaea-specific primers for 16S rDNA gene amplification: 344F (5´- ACG GGG YGC AGC AGG CGC GA-3´) and 915R (5´- GTG CTC CCC CGC CAA TTC CT -3´). Fragments of 577 bp, 317pb and 197pb were obtained from 16S rDNA, aioB and arrA genes respectively. Universal primers 27F and 1492R were used to amplify 16S rDNA in bacterial isolates. 25 isolates belonging to Archaea and Bacteria Domain were obtained; they are related to the Phylum Euryarchaeota, Firmicutes and Proteobacteria. AioB and arrA genes were found in most of the isolates and DNA from the samples (mats, microbialites and biofilm). The best carbon source tested was pyruvate and acetate, being pyruvate better in all cases. Promising results were obtained in the search of organisms able to use arsenic in their bioenergetic metabolism. More studies are underway to try to better understand these very interesting systems.
publishDate 2015
dc.date.none.fl_str_mv 2015
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dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/189405
Extreme-halophiles: their role in the arsenic biogeochemical cycle; XI Congreso Argentino de Microbiología General; Cordoba; Argentina; 2015; 1-3
CONICET Digital
CONICET
url http://hdl.handle.net/11336/189405
identifier_str_mv Extreme-halophiles: their role in the arsenic biogeochemical cycle; XI Congreso Argentino de Microbiología General; Cordoba; Argentina; 2015; 1-3
CONICET Digital
CONICET
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