Reactive Nitrogen Species in Brain after In Vivo Exposure to Arsenic
- Autores
- Bonetto, Julián Gerardo; Perazzo, Juan C.; Puntarulo, Susana Ángela
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Arsenic (As) is a natural pollutant, which exposure is related to avariety of diseases like hypertension, diabetes, cancer andneurodegenerative or neurodevelopment impairment but, the exactmechanism by which As exposure could be toxic is still unclear.Studies employing different models of Astoxicity suggested that Ascould cause ROS generation and antioxidant depletion in biologicalsystems, including controversial data related to RNS generation andnitrosative damage. The hypothesis of the present work is that acute Asexposure could trigger an imbalance in the production of RNS in brain,leading to nitrosative stress and nitrosative-dependent cellular damage.The effect of in vivo exposure to As was studied by histopathology ofthe tissue, NO2- plus NO3- content, NO generation rate employingElectron Paramagnetic Resonance (EPR) techniques, and the content ofnitrotyrosine (NO2-Tyr) of total proteins in rat brain by western blottechniques. The data presented here suggested that the exposure to Asincreased the production of NO and lipid radicals in brain, leading tonitrosative damage to proteins and a depletion of the hippocampalpyramidal cell layer, that could affect functionality of the brain.
Fil: Bonetto, Julián Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Perazzo, Juan C.. Universidad de Buenos Aires; Argentina
Fil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina - Materia
-
ARSENIC
BRAIN
OXIDATIVE STRESS
NITROSATIVE STRESS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/211865
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Reactive Nitrogen Species in Brain after In Vivo Exposure to ArsenicBonetto, Julián GerardoPerazzo, Juan C.Puntarulo, Susana ÁngelaARSENICBRAINOXIDATIVE STRESSNITROSATIVE STRESShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Arsenic (As) is a natural pollutant, which exposure is related to avariety of diseases like hypertension, diabetes, cancer andneurodegenerative or neurodevelopment impairment but, the exactmechanism by which As exposure could be toxic is still unclear.Studies employing different models of Astoxicity suggested that Ascould cause ROS generation and antioxidant depletion in biologicalsystems, including controversial data related to RNS generation andnitrosative damage. The hypothesis of the present work is that acute Asexposure could trigger an imbalance in the production of RNS in brain,leading to nitrosative stress and nitrosative-dependent cellular damage.The effect of in vivo exposure to As was studied by histopathology ofthe tissue, NO2- plus NO3- content, NO generation rate employingElectron Paramagnetic Resonance (EPR) techniques, and the content ofnitrotyrosine (NO2-Tyr) of total proteins in rat brain by western blottechniques. The data presented here suggested that the exposure to Asincreased the production of NO and lipid radicals in brain, leading tonitrosative damage to proteins and a depletion of the hippocampalpyramidal cell layer, that could affect functionality of the brain.Fil: Bonetto, Julián Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Perazzo, Juan C.. Universidad de Buenos Aires; ArgentinaFil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaInternational Journal of Advanced Research2020-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/211865Bonetto, Julián Gerardo; Perazzo, Juan C.; Puntarulo, Susana Ángela; Reactive Nitrogen Species in Brain after In Vivo Exposure to Arsenic; International Journal of Advanced Research; International Journal of Advanced Research; 11-2020; 980-9922320-5407CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.21474/IJAR01/11179info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:39:33Zoai:ri.conicet.gov.ar:11336/211865instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:39:33.268CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Reactive Nitrogen Species in Brain after In Vivo Exposure to Arsenic |
title |
Reactive Nitrogen Species in Brain after In Vivo Exposure to Arsenic |
spellingShingle |
Reactive Nitrogen Species in Brain after In Vivo Exposure to Arsenic Bonetto, Julián Gerardo ARSENIC BRAIN OXIDATIVE STRESS NITROSATIVE STRESS |
title_short |
Reactive Nitrogen Species in Brain after In Vivo Exposure to Arsenic |
title_full |
Reactive Nitrogen Species in Brain after In Vivo Exposure to Arsenic |
title_fullStr |
Reactive Nitrogen Species in Brain after In Vivo Exposure to Arsenic |
title_full_unstemmed |
Reactive Nitrogen Species in Brain after In Vivo Exposure to Arsenic |
title_sort |
Reactive Nitrogen Species in Brain after In Vivo Exposure to Arsenic |
dc.creator.none.fl_str_mv |
Bonetto, Julián Gerardo Perazzo, Juan C. Puntarulo, Susana Ángela |
author |
Bonetto, Julián Gerardo |
author_facet |
Bonetto, Julián Gerardo Perazzo, Juan C. Puntarulo, Susana Ángela |
author_role |
author |
author2 |
Perazzo, Juan C. Puntarulo, Susana Ángela |
author2_role |
author author |
dc.subject.none.fl_str_mv |
ARSENIC BRAIN OXIDATIVE STRESS NITROSATIVE STRESS |
topic |
ARSENIC BRAIN OXIDATIVE STRESS NITROSATIVE STRESS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Arsenic (As) is a natural pollutant, which exposure is related to avariety of diseases like hypertension, diabetes, cancer andneurodegenerative or neurodevelopment impairment but, the exactmechanism by which As exposure could be toxic is still unclear.Studies employing different models of Astoxicity suggested that Ascould cause ROS generation and antioxidant depletion in biologicalsystems, including controversial data related to RNS generation andnitrosative damage. The hypothesis of the present work is that acute Asexposure could trigger an imbalance in the production of RNS in brain,leading to nitrosative stress and nitrosative-dependent cellular damage.The effect of in vivo exposure to As was studied by histopathology ofthe tissue, NO2- plus NO3- content, NO generation rate employingElectron Paramagnetic Resonance (EPR) techniques, and the content ofnitrotyrosine (NO2-Tyr) of total proteins in rat brain by western blottechniques. The data presented here suggested that the exposure to Asincreased the production of NO and lipid radicals in brain, leading tonitrosative damage to proteins and a depletion of the hippocampalpyramidal cell layer, that could affect functionality of the brain. Fil: Bonetto, Julián Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina Fil: Perazzo, Juan C.. Universidad de Buenos Aires; Argentina Fil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina |
description |
Arsenic (As) is a natural pollutant, which exposure is related to avariety of diseases like hypertension, diabetes, cancer andneurodegenerative or neurodevelopment impairment but, the exactmechanism by which As exposure could be toxic is still unclear.Studies employing different models of Astoxicity suggested that Ascould cause ROS generation and antioxidant depletion in biologicalsystems, including controversial data related to RNS generation andnitrosative damage. The hypothesis of the present work is that acute Asexposure could trigger an imbalance in the production of RNS in brain,leading to nitrosative stress and nitrosative-dependent cellular damage.The effect of in vivo exposure to As was studied by histopathology ofthe tissue, NO2- plus NO3- content, NO generation rate employingElectron Paramagnetic Resonance (EPR) techniques, and the content ofnitrotyrosine (NO2-Tyr) of total proteins in rat brain by western blottechniques. The data presented here suggested that the exposure to Asincreased the production of NO and lipid radicals in brain, leading tonitrosative damage to proteins and a depletion of the hippocampalpyramidal cell layer, that could affect functionality of the brain. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/211865 Bonetto, Julián Gerardo; Perazzo, Juan C.; Puntarulo, Susana Ángela; Reactive Nitrogen Species in Brain after In Vivo Exposure to Arsenic; International Journal of Advanced Research; International Journal of Advanced Research; 11-2020; 980-992 2320-5407 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/211865 |
identifier_str_mv |
Bonetto, Julián Gerardo; Perazzo, Juan C.; Puntarulo, Susana Ángela; Reactive Nitrogen Species in Brain after In Vivo Exposure to Arsenic; International Journal of Advanced Research; International Journal of Advanced Research; 11-2020; 980-992 2320-5407 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.21474/IJAR01/11179 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
International Journal of Advanced Research |
publisher.none.fl_str_mv |
International Journal of Advanced Research |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |