Reactive Nitrogen Species in Brain after In Vivo Exposure to Arsenic

Autores
Bonetto, Julián Gerardo; Perazzo, Juan C.; Puntarulo, Susana Ángela
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Arsenic (As) is a natural pollutant, which exposure is related to avariety of diseases like hypertension, diabetes, cancer andneurodegenerative or neurodevelopment impairment but, the exactmechanism by which As exposure could be toxic is still unclear.Studies employing different models of Astoxicity suggested that Ascould cause ROS generation and antioxidant depletion in biologicalsystems, including controversial data related to RNS generation andnitrosative damage. The hypothesis of the present work is that acute Asexposure could trigger an imbalance in the production of RNS in brain,leading to nitrosative stress and nitrosative-dependent cellular damage.The effect of in vivo exposure to As was studied by histopathology ofthe tissue, NO2- plus NO3- content, NO generation rate employingElectron Paramagnetic Resonance (EPR) techniques, and the content ofnitrotyrosine (NO2-Tyr) of total proteins in rat brain by western blottechniques. The data presented here suggested that the exposure to Asincreased the production of NO and lipid radicals in brain, leading tonitrosative damage to proteins and a depletion of the hippocampalpyramidal cell layer, that could affect functionality of the brain.
Fil: Bonetto, Julián Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Perazzo, Juan C.. Universidad de Buenos Aires; Argentina
Fil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Materia
ARSENIC
BRAIN
OXIDATIVE STRESS
NITROSATIVE STRESS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/211865
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Reactive Nitrogen Species in Brain after In Vivo Exposure to ArsenicBonetto, Julián GerardoPerazzo, Juan C.Puntarulo, Susana ÁngelaARSENICBRAINOXIDATIVE STRESSNITROSATIVE STRESShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Arsenic (As) is a natural pollutant, which exposure is related to avariety of diseases like hypertension, diabetes, cancer andneurodegenerative or neurodevelopment impairment but, the exactmechanism by which As exposure could be toxic is still unclear.Studies employing different models of Astoxicity suggested that Ascould cause ROS generation and antioxidant depletion in biologicalsystems, including controversial data related to RNS generation andnitrosative damage. The hypothesis of the present work is that acute Asexposure could trigger an imbalance in the production of RNS in brain,leading to nitrosative stress and nitrosative-dependent cellular damage.The effect of in vivo exposure to As was studied by histopathology ofthe tissue, NO2- plus NO3- content, NO generation rate employingElectron Paramagnetic Resonance (EPR) techniques, and the content ofnitrotyrosine (NO2-Tyr) of total proteins in rat brain by western blottechniques. The data presented here suggested that the exposure to Asincreased the production of NO and lipid radicals in brain, leading tonitrosative damage to proteins and a depletion of the hippocampalpyramidal cell layer, that could affect functionality of the brain.Fil: Bonetto, Julián Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Perazzo, Juan C.. Universidad de Buenos Aires; ArgentinaFil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaInternational Journal of Advanced Research2020-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/211865Bonetto, Julián Gerardo; Perazzo, Juan C.; Puntarulo, Susana Ángela; Reactive Nitrogen Species in Brain after In Vivo Exposure to Arsenic; International Journal of Advanced Research; International Journal of Advanced Research; 11-2020; 980-9922320-5407CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.21474/IJAR01/11179info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:11:50Zoai:ri.conicet.gov.ar:11336/211865instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:11:50.368CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Reactive Nitrogen Species in Brain after In Vivo Exposure to Arsenic
title Reactive Nitrogen Species in Brain after In Vivo Exposure to Arsenic
spellingShingle Reactive Nitrogen Species in Brain after In Vivo Exposure to Arsenic
Bonetto, Julián Gerardo
ARSENIC
BRAIN
OXIDATIVE STRESS
NITROSATIVE STRESS
title_short Reactive Nitrogen Species in Brain after In Vivo Exposure to Arsenic
title_full Reactive Nitrogen Species in Brain after In Vivo Exposure to Arsenic
title_fullStr Reactive Nitrogen Species in Brain after In Vivo Exposure to Arsenic
title_full_unstemmed Reactive Nitrogen Species in Brain after In Vivo Exposure to Arsenic
title_sort Reactive Nitrogen Species in Brain after In Vivo Exposure to Arsenic
dc.creator.none.fl_str_mv Bonetto, Julián Gerardo
Perazzo, Juan C.
Puntarulo, Susana Ángela
author Bonetto, Julián Gerardo
author_facet Bonetto, Julián Gerardo
Perazzo, Juan C.
Puntarulo, Susana Ángela
author_role author
author2 Perazzo, Juan C.
Puntarulo, Susana Ángela
author2_role author
author
dc.subject.none.fl_str_mv ARSENIC
BRAIN
OXIDATIVE STRESS
NITROSATIVE STRESS
topic ARSENIC
BRAIN
OXIDATIVE STRESS
NITROSATIVE STRESS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Arsenic (As) is a natural pollutant, which exposure is related to avariety of diseases like hypertension, diabetes, cancer andneurodegenerative or neurodevelopment impairment but, the exactmechanism by which As exposure could be toxic is still unclear.Studies employing different models of Astoxicity suggested that Ascould cause ROS generation and antioxidant depletion in biologicalsystems, including controversial data related to RNS generation andnitrosative damage. The hypothesis of the present work is that acute Asexposure could trigger an imbalance in the production of RNS in brain,leading to nitrosative stress and nitrosative-dependent cellular damage.The effect of in vivo exposure to As was studied by histopathology ofthe tissue, NO2- plus NO3- content, NO generation rate employingElectron Paramagnetic Resonance (EPR) techniques, and the content ofnitrotyrosine (NO2-Tyr) of total proteins in rat brain by western blottechniques. The data presented here suggested that the exposure to Asincreased the production of NO and lipid radicals in brain, leading tonitrosative damage to proteins and a depletion of the hippocampalpyramidal cell layer, that could affect functionality of the brain.
Fil: Bonetto, Julián Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Perazzo, Juan C.. Universidad de Buenos Aires; Argentina
Fil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
description Arsenic (As) is a natural pollutant, which exposure is related to avariety of diseases like hypertension, diabetes, cancer andneurodegenerative or neurodevelopment impairment but, the exactmechanism by which As exposure could be toxic is still unclear.Studies employing different models of Astoxicity suggested that Ascould cause ROS generation and antioxidant depletion in biologicalsystems, including controversial data related to RNS generation andnitrosative damage. The hypothesis of the present work is that acute Asexposure could trigger an imbalance in the production of RNS in brain,leading to nitrosative stress and nitrosative-dependent cellular damage.The effect of in vivo exposure to As was studied by histopathology ofthe tissue, NO2- plus NO3- content, NO generation rate employingElectron Paramagnetic Resonance (EPR) techniques, and the content ofnitrotyrosine (NO2-Tyr) of total proteins in rat brain by western blottechniques. The data presented here suggested that the exposure to Asincreased the production of NO and lipid radicals in brain, leading tonitrosative damage to proteins and a depletion of the hippocampalpyramidal cell layer, that could affect functionality of the brain.
publishDate 2020
dc.date.none.fl_str_mv 2020-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/211865
Bonetto, Julián Gerardo; Perazzo, Juan C.; Puntarulo, Susana Ángela; Reactive Nitrogen Species in Brain after In Vivo Exposure to Arsenic; International Journal of Advanced Research; International Journal of Advanced Research; 11-2020; 980-992
2320-5407
CONICET Digital
CONICET
url http://hdl.handle.net/11336/211865
identifier_str_mv Bonetto, Julián Gerardo; Perazzo, Juan C.; Puntarulo, Susana Ángela; Reactive Nitrogen Species in Brain after In Vivo Exposure to Arsenic; International Journal of Advanced Research; International Journal of Advanced Research; 11-2020; 980-992
2320-5407
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.21474/IJAR01/11179
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv International Journal of Advanced Research
publisher.none.fl_str_mv International Journal of Advanced Research
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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