Relationship between oxidative and nitrosative stress induced by gentamicin and ciprofloxacin in bacteria

Autores
Galera, Ivana Laura Delia; Paraje, Maria Gabriela; Paez, Paulina Laura
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Oxygen is essential to the life of the aerobic organisms, and most is used for the intracellular generation of energy, where several molecules are formed by partial reduction of oxygen. Because of its short half-life, low selectivity and high reactivity, biological targets of reactive oxygen species (ROS) include various components such as proteins, nucleic acids and lipids. The purpose of this work was to establish a relationship between ROS and reactive nitrogen intermediates (RNI) generation in bacteria exposed to antibiotics. ROS was determined by the reduction of nitro blue tetrazolium (NBT). In aqueous solutions, nitric oxide is converted to nitrite (NO2-), this was determined using a non-enzymatic colorimetric assay. NBT studies indicated stimulation of oxidative stress with an increase of ROS generated by the antibiotic in the bacteria. We observed an increase in the production of NO2- dose-dependent with CIP and GEN. The determination of ROS and RNI allowed establish differences in the alteration of oxidative metabolism in susceptible and resistant strains. Resistance to CIP and GEN may be related to the decreased production of ROS and RNI and consequently, a smaller macromolecules bacterial oxidation. These results contribute to a better understanding of the ROS and RNI regulation.
Fil: Galera, Ivana Laura Delia. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina
Fil: Paraje, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Paez, Paulina Laura. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Oxidative Stress
Bacteria
Nitrosative Stress
Antibiotics
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/19246
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/19246
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Relationship between oxidative and nitrosative stress induced by gentamicin and ciprofloxacin in bacteriaGalera, Ivana Laura DeliaParaje, Maria GabrielaPaez, Paulina LauraOxidative StressBacteriaNitrosative StressAntibioticshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Oxygen is essential to the life of the aerobic organisms, and most is used for the intracellular generation of energy, where several molecules are formed by partial reduction of oxygen. Because of its short half-life, low selectivity and high reactivity, biological targets of reactive oxygen species (ROS) include various components such as proteins, nucleic acids and lipids. The purpose of this work was to establish a relationship between ROS and reactive nitrogen intermediates (RNI) generation in bacteria exposed to antibiotics. ROS was determined by the reduction of nitro blue tetrazolium (NBT). In aqueous solutions, nitric oxide is converted to nitrite (NO2-), this was determined using a non-enzymatic colorimetric assay. NBT studies indicated stimulation of oxidative stress with an increase of ROS generated by the antibiotic in the bacteria. We observed an increase in the production of NO2- dose-dependent with CIP and GEN. The determination of ROS and RNI allowed establish differences in the alteration of oxidative metabolism in susceptible and resistant strains. Resistance to CIP and GEN may be related to the decreased production of ROS and RNI and consequently, a smaller macromolecules bacterial oxidation. These results contribute to a better understanding of the ROS and RNI regulation.Fil: Galera, Ivana Laura Delia. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; ArgentinaFil: Paraje, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Paez, Paulina Laura. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaInternational Knowledge Press2016-02-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/19246Galera, Ivana Laura Delia; Paraje, Maria Gabriela; Paez, Paulina Laura; Relationship between oxidative and nitrosative stress induced by gentamicin and ciprofloxacin in bacteria; International Knowledge Press; Journal of Biology and Nature; 5; 3; 18-2-2016; 2395-53762395-53762395-5384CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.ikpress.org/abstract/5319info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:11Zoai:ri.conicet.gov.ar:11336/19246instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:11.495CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Relationship between oxidative and nitrosative stress induced by gentamicin and ciprofloxacin in bacteria
title Relationship between oxidative and nitrosative stress induced by gentamicin and ciprofloxacin in bacteria
spellingShingle Relationship between oxidative and nitrosative stress induced by gentamicin and ciprofloxacin in bacteria
Galera, Ivana Laura Delia
Oxidative Stress
Bacteria
Nitrosative Stress
Antibiotics
title_short Relationship between oxidative and nitrosative stress induced by gentamicin and ciprofloxacin in bacteria
title_full Relationship between oxidative and nitrosative stress induced by gentamicin and ciprofloxacin in bacteria
title_fullStr Relationship between oxidative and nitrosative stress induced by gentamicin and ciprofloxacin in bacteria
title_full_unstemmed Relationship between oxidative and nitrosative stress induced by gentamicin and ciprofloxacin in bacteria
title_sort Relationship between oxidative and nitrosative stress induced by gentamicin and ciprofloxacin in bacteria
dc.creator.none.fl_str_mv Galera, Ivana Laura Delia
Paraje, Maria Gabriela
Paez, Paulina Laura
author Galera, Ivana Laura Delia
author_facet Galera, Ivana Laura Delia
Paraje, Maria Gabriela
Paez, Paulina Laura
author_role author
author2 Paraje, Maria Gabriela
Paez, Paulina Laura
author2_role author
author
dc.subject.none.fl_str_mv Oxidative Stress
Bacteria
Nitrosative Stress
Antibiotics
topic Oxidative Stress
Bacteria
Nitrosative Stress
Antibiotics
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Oxygen is essential to the life of the aerobic organisms, and most is used for the intracellular generation of energy, where several molecules are formed by partial reduction of oxygen. Because of its short half-life, low selectivity and high reactivity, biological targets of reactive oxygen species (ROS) include various components such as proteins, nucleic acids and lipids. The purpose of this work was to establish a relationship between ROS and reactive nitrogen intermediates (RNI) generation in bacteria exposed to antibiotics. ROS was determined by the reduction of nitro blue tetrazolium (NBT). In aqueous solutions, nitric oxide is converted to nitrite (NO2-), this was determined using a non-enzymatic colorimetric assay. NBT studies indicated stimulation of oxidative stress with an increase of ROS generated by the antibiotic in the bacteria. We observed an increase in the production of NO2- dose-dependent with CIP and GEN. The determination of ROS and RNI allowed establish differences in the alteration of oxidative metabolism in susceptible and resistant strains. Resistance to CIP and GEN may be related to the decreased production of ROS and RNI and consequently, a smaller macromolecules bacterial oxidation. These results contribute to a better understanding of the ROS and RNI regulation.
Fil: Galera, Ivana Laura Delia. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina
Fil: Paraje, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Paez, Paulina Laura. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Oxygen is essential to the life of the aerobic organisms, and most is used for the intracellular generation of energy, where several molecules are formed by partial reduction of oxygen. Because of its short half-life, low selectivity and high reactivity, biological targets of reactive oxygen species (ROS) include various components such as proteins, nucleic acids and lipids. The purpose of this work was to establish a relationship between ROS and reactive nitrogen intermediates (RNI) generation in bacteria exposed to antibiotics. ROS was determined by the reduction of nitro blue tetrazolium (NBT). In aqueous solutions, nitric oxide is converted to nitrite (NO2-), this was determined using a non-enzymatic colorimetric assay. NBT studies indicated stimulation of oxidative stress with an increase of ROS generated by the antibiotic in the bacteria. We observed an increase in the production of NO2- dose-dependent with CIP and GEN. The determination of ROS and RNI allowed establish differences in the alteration of oxidative metabolism in susceptible and resistant strains. Resistance to CIP and GEN may be related to the decreased production of ROS and RNI and consequently, a smaller macromolecules bacterial oxidation. These results contribute to a better understanding of the ROS and RNI regulation.
publishDate 2016
dc.date.none.fl_str_mv 2016-02-18
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/19246
Galera, Ivana Laura Delia; Paraje, Maria Gabriela; Paez, Paulina Laura; Relationship between oxidative and nitrosative stress induced by gentamicin and ciprofloxacin in bacteria; International Knowledge Press; Journal of Biology and Nature; 5; 3; 18-2-2016; 2395-5376
2395-5376
2395-5384
CONICET Digital
CONICET
url http://hdl.handle.net/11336/19246
identifier_str_mv Galera, Ivana Laura Delia; Paraje, Maria Gabriela; Paez, Paulina Laura; Relationship between oxidative and nitrosative stress induced by gentamicin and ciprofloxacin in bacteria; International Knowledge Press; Journal of Biology and Nature; 5; 3; 18-2-2016; 2395-5376
2395-5376
2395-5384
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ikpress.org/abstract/5319
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv International Knowledge Press
publisher.none.fl_str_mv International Knowledge Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268958919491584
score 13.13397

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