Oxidative balance in brain after chronic exposure to arsenic

Autores
Bonetto, Julián Gerardo; Puntarulo, Susana Ángela
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effect of chronic As exposure in brain, the specific oxidantsproduction and oxidative stress still needs more attention. In the presentstudy, male wistar rats were chronically exposed to As bythe drinkingwater during 60 days. The rats receiving a dose of 10 mg As/l showed a9- and 18-fold increase in the content of As in blood and brain,respectively, as compared to the control group. Histopathologicalchanges were detected in brain samples isolated from animals treatedwith Asas compared to control animals. The oxidation rate of 2ʹ,7ʹ-Dichlorofluorescein diacetate (DCFH-DA) showed no significantchanges after the treatment. A significant increase (13%) in the steadystate concentration of ascorbyl radical (A?) was determined in the brainisolated from animals exposed to 50 mg As/l. The lipid radical (LR?)generation rate and the content of malondialdehyde (MDA) wereincreased by 34% and 32%, respectively in brain isolated from ratsreceiving 50 mg As/l. The content of ascorbate (AH-) in brain was notaffected by the exposure to As.However, the content of glutathione(GSH) and the lipophilic antioxidant α-tocopherol (α-T), weresignificantly decreased after As supplementation, as compared tocontrol brains. The A?/AH- content ratio in rat brain showed no changesassociated to the As supplementation. Nevertheless, a significantincrease of 44% and 55% was determined in the LR?/α-T and MDA/α-Tcontent ratios, respectively, suggesting that the oxidative imbalance inthe lipophilic cellular medium could be the primarily effect of Asassociated to membrane damage.
Fil: Bonetto, Julián Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Materia
ANTIOXIDANTS
ARSENIC
BRAIN
OXIDATIVE BALANCE IN THE LIPOPHILIC CELLULAR MEDIUM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/203989
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Oxidative balance in brain after chronic exposure to arsenicBonetto, Julián GerardoPuntarulo, Susana ÁngelaANTIOXIDANTSARSENICBRAINOXIDATIVE BALANCE IN THE LIPOPHILIC CELLULAR MEDIUMhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The effect of chronic As exposure in brain, the specific oxidantsproduction and oxidative stress still needs more attention. In the presentstudy, male wistar rats were chronically exposed to As bythe drinkingwater during 60 days. The rats receiving a dose of 10 mg As/l showed a9- and 18-fold increase in the content of As in blood and brain,respectively, as compared to the control group. Histopathologicalchanges were detected in brain samples isolated from animals treatedwith Asas compared to control animals. The oxidation rate of 2ʹ,7ʹ-Dichlorofluorescein diacetate (DCFH-DA) showed no significantchanges after the treatment. A significant increase (13%) in the steadystate concentration of ascorbyl radical (A?) was determined in the brainisolated from animals exposed to 50 mg As/l. The lipid radical (LR?)generation rate and the content of malondialdehyde (MDA) wereincreased by 34% and 32%, respectively in brain isolated from ratsreceiving 50 mg As/l. The content of ascorbate (AH-) in brain was notaffected by the exposure to As.However, the content of glutathione(GSH) and the lipophilic antioxidant α-tocopherol (α-T), weresignificantly decreased after As supplementation, as compared tocontrol brains. The A?/AH- content ratio in rat brain showed no changesassociated to the As supplementation. Nevertheless, a significantincrease of 44% and 55% was determined in the LR?/α-T and MDA/α-Tcontent ratios, respectively, suggesting that the oxidative imbalance inthe lipophilic cellular medium could be the primarily effect of Asassociated to membrane damage.Fil: Bonetto, Julián Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaInternational Journal of Advanced Research2022-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/203989Bonetto, Julián Gerardo; Puntarulo, Susana Ángela; Oxidative balance in brain after chronic exposure to arsenic; International Journal of Advanced Research; International Journal of Advanced Research; 10; 8-2022; 980-9912320-5407CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.21474/IJAR01/15258info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:24Zoai:ri.conicet.gov.ar:11336/203989instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:25.251CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Oxidative balance in brain after chronic exposure to arsenic
title Oxidative balance in brain after chronic exposure to arsenic
spellingShingle Oxidative balance in brain after chronic exposure to arsenic
Bonetto, Julián Gerardo
ANTIOXIDANTS
ARSENIC
BRAIN
OXIDATIVE BALANCE IN THE LIPOPHILIC CELLULAR MEDIUM
title_short Oxidative balance in brain after chronic exposure to arsenic
title_full Oxidative balance in brain after chronic exposure to arsenic
title_fullStr Oxidative balance in brain after chronic exposure to arsenic
title_full_unstemmed Oxidative balance in brain after chronic exposure to arsenic
title_sort Oxidative balance in brain after chronic exposure to arsenic
dc.creator.none.fl_str_mv Bonetto, Julián Gerardo
Puntarulo, Susana Ángela
author Bonetto, Julián Gerardo
author_facet Bonetto, Julián Gerardo
Puntarulo, Susana Ángela
author_role author
author2 Puntarulo, Susana Ángela
author2_role author
dc.subject.none.fl_str_mv ANTIOXIDANTS
ARSENIC
BRAIN
OXIDATIVE BALANCE IN THE LIPOPHILIC CELLULAR MEDIUM
topic ANTIOXIDANTS
ARSENIC
BRAIN
OXIDATIVE BALANCE IN THE LIPOPHILIC CELLULAR MEDIUM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The effect of chronic As exposure in brain, the specific oxidantsproduction and oxidative stress still needs more attention. In the presentstudy, male wistar rats were chronically exposed to As bythe drinkingwater during 60 days. The rats receiving a dose of 10 mg As/l showed a9- and 18-fold increase in the content of As in blood and brain,respectively, as compared to the control group. Histopathologicalchanges were detected in brain samples isolated from animals treatedwith Asas compared to control animals. The oxidation rate of 2ʹ,7ʹ-Dichlorofluorescein diacetate (DCFH-DA) showed no significantchanges after the treatment. A significant increase (13%) in the steadystate concentration of ascorbyl radical (A?) was determined in the brainisolated from animals exposed to 50 mg As/l. The lipid radical (LR?)generation rate and the content of malondialdehyde (MDA) wereincreased by 34% and 32%, respectively in brain isolated from ratsreceiving 50 mg As/l. The content of ascorbate (AH-) in brain was notaffected by the exposure to As.However, the content of glutathione(GSH) and the lipophilic antioxidant α-tocopherol (α-T), weresignificantly decreased after As supplementation, as compared tocontrol brains. The A?/AH- content ratio in rat brain showed no changesassociated to the As supplementation. Nevertheless, a significantincrease of 44% and 55% was determined in the LR?/α-T and MDA/α-Tcontent ratios, respectively, suggesting that the oxidative imbalance inthe lipophilic cellular medium could be the primarily effect of Asassociated to membrane damage.
Fil: Bonetto, Julián Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
description The effect of chronic As exposure in brain, the specific oxidantsproduction and oxidative stress still needs more attention. In the presentstudy, male wistar rats were chronically exposed to As bythe drinkingwater during 60 days. The rats receiving a dose of 10 mg As/l showed a9- and 18-fold increase in the content of As in blood and brain,respectively, as compared to the control group. Histopathologicalchanges were detected in brain samples isolated from animals treatedwith Asas compared to control animals. The oxidation rate of 2ʹ,7ʹ-Dichlorofluorescein diacetate (DCFH-DA) showed no significantchanges after the treatment. A significant increase (13%) in the steadystate concentration of ascorbyl radical (A?) was determined in the brainisolated from animals exposed to 50 mg As/l. The lipid radical (LR?)generation rate and the content of malondialdehyde (MDA) wereincreased by 34% and 32%, respectively in brain isolated from ratsreceiving 50 mg As/l. The content of ascorbate (AH-) in brain was notaffected by the exposure to As.However, the content of glutathione(GSH) and the lipophilic antioxidant α-tocopherol (α-T), weresignificantly decreased after As supplementation, as compared tocontrol brains. The A?/AH- content ratio in rat brain showed no changesassociated to the As supplementation. Nevertheless, a significantincrease of 44% and 55% was determined in the LR?/α-T and MDA/α-Tcontent ratios, respectively, suggesting that the oxidative imbalance inthe lipophilic cellular medium could be the primarily effect of Asassociated to membrane damage.
publishDate 2022
dc.date.none.fl_str_mv 2022-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/203989
Bonetto, Julián Gerardo; Puntarulo, Susana Ángela; Oxidative balance in brain after chronic exposure to arsenic; International Journal of Advanced Research; International Journal of Advanced Research; 10; 8-2022; 980-991
2320-5407
CONICET Digital
CONICET
url http://hdl.handle.net/11336/203989
identifier_str_mv Bonetto, Julián Gerardo; Puntarulo, Susana Ángela; Oxidative balance in brain after chronic exposure to arsenic; International Journal of Advanced Research; International Journal of Advanced Research; 10; 8-2022; 980-991
2320-5407
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.21474/IJAR01/15258
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv International Journal of Advanced Research
publisher.none.fl_str_mv International Journal of Advanced Research
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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