Neurogenetics of confinement-induced behavioral alterations.

Autores
Heredia, Fabiana; Zanini, Rebeca; Macedo, André; Varela, Ednilson; Menezes, Juliane; Ibarra, Julieta; Garelli, Andres; Gontijo, Alisson
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The molecular and neural mechanisms underlying the translation of physical confinement to internal state changes and how these alter behavior are poorly understood and very difficult to study in natural settings due to an excessive number of possible confounding factors. Innate behaviors, which are complex, genetically-encoded behaviors that proceed with a predictable sequence, provide a unique window to study the effects of confinement on behavior. Adding this to the power of a genetically tractable model organism, we aim to help unravel both the molecular and cellular foundations of behaviorally-relevant, confinement-induced changes in internal states. Here, we start to genetically dissect the wing expansion behavior that Drosophila flies perform upon eclosing from their puparium. The execution of this behavior, which is triggered by the hormone Bursicon, is strongly negatively-regulated by spatial confinement. Namely, wing expansion behavior occurs within 30 min when animals eclose in normal, unconfined conditions, yet it is delayed to > 180 min upon eclosion into a confined environment. The molecular mechanisms controlling this wing expansion decision process upon confinement are poorly understood. By chance, we found a background mutation segregating in Drosophila stocks where wing expansion behavior is severely compromised specifically under confinement, not under normal unconfined conditions. We are mapping this mutation in the hope that it provides a unique entrance into the molecular and neural underpinnings of confinement-induced behavioral control. In parallel, we have performed two studies to identify factors that are affected in the central nervous system of animals undergoing spatial confinement: a genome wide time-series transcriptomic study of confined and unconfined animals and a small-scale genetic screen for neuropeptides and receptors affecting this confinement-induced behavioral response. We will present the preliminary analyses of these studies. We hope that these approaches will help clarify how spatial confinement alters wing expansion behavior, and provide further insight on how environmental changes affect behavior and health
Fil: Heredia, Fabiana. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Zanini, Rebeca. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Macedo, André. German Cancer Research Center, Heidelberg; Alemania
Fil: Varela, Ednilson. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Menezes, Juliane. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Ibarra, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Garelli, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Gontijo, Alisson. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
European Drosophila Research Conference
Lyon
Francia
Institut de Génomique Fonctionnelle de Lyon
Laboratoire de Biologie et Modélisation de la Cellule
Ecole Normale Supérieure de Lyon
Materia
INNATE BEHAVIOR
GENETIC SCREEN
WING EXPANSION
DROSOPHILA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/240210
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Neurogenetics of confinement-induced behavioral alterations.Heredia, FabianaZanini, RebecaMacedo, AndréVarela, EdnilsonMenezes, JulianeIbarra, JulietaGarelli, AndresGontijo, AlissonINNATE BEHAVIORGENETIC SCREENWING EXPANSIONDROSOPHILAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The molecular and neural mechanisms underlying the translation of physical confinement to internal state changes and how these alter behavior are poorly understood and very difficult to study in natural settings due to an excessive number of possible confounding factors. Innate behaviors, which are complex, genetically-encoded behaviors that proceed with a predictable sequence, provide a unique window to study the effects of confinement on behavior. Adding this to the power of a genetically tractable model organism, we aim to help unravel both the molecular and cellular foundations of behaviorally-relevant, confinement-induced changes in internal states. Here, we start to genetically dissect the wing expansion behavior that Drosophila flies perform upon eclosing from their puparium. The execution of this behavior, which is triggered by the hormone Bursicon, is strongly negatively-regulated by spatial confinement. Namely, wing expansion behavior occurs within 30 min when animals eclose in normal, unconfined conditions, yet it is delayed to > 180 min upon eclosion into a confined environment. The molecular mechanisms controlling this wing expansion decision process upon confinement are poorly understood. By chance, we found a background mutation segregating in Drosophila stocks where wing expansion behavior is severely compromised specifically under confinement, not under normal unconfined conditions. We are mapping this mutation in the hope that it provides a unique entrance into the molecular and neural underpinnings of confinement-induced behavioral control. In parallel, we have performed two studies to identify factors that are affected in the central nervous system of animals undergoing spatial confinement: a genome wide time-series transcriptomic study of confined and unconfined animals and a small-scale genetic screen for neuropeptides and receptors affecting this confinement-induced behavioral response. We will present the preliminary analyses of these studies. We hope that these approaches will help clarify how spatial confinement alters wing expansion behavior, and provide further insight on how environmental changes affect behavior and healthFil: Heredia, Fabiana. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; PortugalFil: Zanini, Rebeca. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; PortugalFil: Macedo, André. German Cancer Research Center, Heidelberg; AlemaniaFil: Varela, Ednilson. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; PortugalFil: Menezes, Juliane. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; PortugalFil: Ibarra, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Garelli, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; PortugalFil: Gontijo, Alisson. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; PortugalEuropean Drosophila Research ConferenceLyonFranciaInstitut de Génomique Fonctionnelle de LyonLaboratoire de Biologie et Modélisation de la CelluleEcole Normale Supérieure de LyonEuropean Drosophila Research Conference2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/240210Neurogenetics of confinement-induced behavioral alterations.; European Drosophila Research Conference; Lyon; Francia; 2023; 444-445CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://edrclyon.sciencesconf.org/resource/page/id/22Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:00:06Zoai:ri.conicet.gov.ar:11336/240210instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:00:06.977CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Neurogenetics of confinement-induced behavioral alterations.
title Neurogenetics of confinement-induced behavioral alterations.
spellingShingle Neurogenetics of confinement-induced behavioral alterations.
Heredia, Fabiana
INNATE BEHAVIOR
GENETIC SCREEN
WING EXPANSION
DROSOPHILA
title_short Neurogenetics of confinement-induced behavioral alterations.
title_full Neurogenetics of confinement-induced behavioral alterations.
title_fullStr Neurogenetics of confinement-induced behavioral alterations.
title_full_unstemmed Neurogenetics of confinement-induced behavioral alterations.
title_sort Neurogenetics of confinement-induced behavioral alterations.
dc.creator.none.fl_str_mv Heredia, Fabiana
Zanini, Rebeca
Macedo, André
Varela, Ednilson
Menezes, Juliane
Ibarra, Julieta
Garelli, Andres
Gontijo, Alisson
author Heredia, Fabiana
author_facet Heredia, Fabiana
Zanini, Rebeca
Macedo, André
Varela, Ednilson
Menezes, Juliane
Ibarra, Julieta
Garelli, Andres
Gontijo, Alisson
author_role author
author2 Zanini, Rebeca
Macedo, André
Varela, Ednilson
Menezes, Juliane
Ibarra, Julieta
Garelli, Andres
Gontijo, Alisson
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv INNATE BEHAVIOR
GENETIC SCREEN
WING EXPANSION
DROSOPHILA
topic INNATE BEHAVIOR
GENETIC SCREEN
WING EXPANSION
DROSOPHILA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The molecular and neural mechanisms underlying the translation of physical confinement to internal state changes and how these alter behavior are poorly understood and very difficult to study in natural settings due to an excessive number of possible confounding factors. Innate behaviors, which are complex, genetically-encoded behaviors that proceed with a predictable sequence, provide a unique window to study the effects of confinement on behavior. Adding this to the power of a genetically tractable model organism, we aim to help unravel both the molecular and cellular foundations of behaviorally-relevant, confinement-induced changes in internal states. Here, we start to genetically dissect the wing expansion behavior that Drosophila flies perform upon eclosing from their puparium. The execution of this behavior, which is triggered by the hormone Bursicon, is strongly negatively-regulated by spatial confinement. Namely, wing expansion behavior occurs within 30 min when animals eclose in normal, unconfined conditions, yet it is delayed to > 180 min upon eclosion into a confined environment. The molecular mechanisms controlling this wing expansion decision process upon confinement are poorly understood. By chance, we found a background mutation segregating in Drosophila stocks where wing expansion behavior is severely compromised specifically under confinement, not under normal unconfined conditions. We are mapping this mutation in the hope that it provides a unique entrance into the molecular and neural underpinnings of confinement-induced behavioral control. In parallel, we have performed two studies to identify factors that are affected in the central nervous system of animals undergoing spatial confinement: a genome wide time-series transcriptomic study of confined and unconfined animals and a small-scale genetic screen for neuropeptides and receptors affecting this confinement-induced behavioral response. We will present the preliminary analyses of these studies. We hope that these approaches will help clarify how spatial confinement alters wing expansion behavior, and provide further insight on how environmental changes affect behavior and health
Fil: Heredia, Fabiana. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Zanini, Rebeca. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Macedo, André. German Cancer Research Center, Heidelberg; Alemania
Fil: Varela, Ednilson. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Menezes, Juliane. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Ibarra, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Garelli, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Gontijo, Alisson. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
European Drosophila Research Conference
Lyon
Francia
Institut de Génomique Fonctionnelle de Lyon
Laboratoire de Biologie et Modélisation de la Cellule
Ecole Normale Supérieure de Lyon
description The molecular and neural mechanisms underlying the translation of physical confinement to internal state changes and how these alter behavior are poorly understood and very difficult to study in natural settings due to an excessive number of possible confounding factors. Innate behaviors, which are complex, genetically-encoded behaviors that proceed with a predictable sequence, provide a unique window to study the effects of confinement on behavior. Adding this to the power of a genetically tractable model organism, we aim to help unravel both the molecular and cellular foundations of behaviorally-relevant, confinement-induced changes in internal states. Here, we start to genetically dissect the wing expansion behavior that Drosophila flies perform upon eclosing from their puparium. The execution of this behavior, which is triggered by the hormone Bursicon, is strongly negatively-regulated by spatial confinement. Namely, wing expansion behavior occurs within 30 min when animals eclose in normal, unconfined conditions, yet it is delayed to > 180 min upon eclosion into a confined environment. The molecular mechanisms controlling this wing expansion decision process upon confinement are poorly understood. By chance, we found a background mutation segregating in Drosophila stocks where wing expansion behavior is severely compromised specifically under confinement, not under normal unconfined conditions. We are mapping this mutation in the hope that it provides a unique entrance into the molecular and neural underpinnings of confinement-induced behavioral control. In parallel, we have performed two studies to identify factors that are affected in the central nervous system of animals undergoing spatial confinement: a genome wide time-series transcriptomic study of confined and unconfined animals and a small-scale genetic screen for neuropeptides and receptors affecting this confinement-induced behavioral response. We will present the preliminary analyses of these studies. We hope that these approaches will help clarify how spatial confinement alters wing expansion behavior, and provide further insight on how environmental changes affect behavior and health
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/240210
Neurogenetics of confinement-induced behavioral alterations.; European Drosophila Research Conference; Lyon; Francia; 2023; 444-445
CONICET Digital
CONICET
url http://hdl.handle.net/11336/240210
identifier_str_mv Neurogenetics of confinement-induced behavioral alterations.; European Drosophila Research Conference; Lyon; Francia; 2023; 444-445
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://edrclyon.sciencesconf.org/resource/page/id/22
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv European Drosophila Research Conference
publisher.none.fl_str_mv European Drosophila Research Conference
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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