In vivo dissection of behaviorally-relevant neuronal relaxin signaling pathway

Autores
Zanini, Rebeca; Heredia, Fabiana; Ibarra, Julieta; Volonté, Yanel Andrea; Gontijo, Alisson; Garelli, Andres
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Relaxin-like peptides (relaxins) belong to the insulin-like family of peptides. Instead of acting via receptor tyrosine kinases as insulin and insulin-like growth factors do, relaxins typically exert their biological effects by binding to G protein-coupled receptors. The relaxin signaling pathway is found both in invertebrates and vertebrates, including humans, and plays important roles in the reproductive, circulatory, skeletal, renal, and nervous systems. The therapeutic potential of human relaxins, for instance, are being explored due to their vasodilator, antifibrotic, and antidepressant properties. However, the biology of relaxin receptors is not fully understood. Here, our primary aim is to identify new conserved regulatory mechanisms of relaxin receptor activity. For this, we are using a relaxin-receptor-dependent Drosophila phenotype to screen for new relaxin pathway components and regulators. In Drosophila, lack of the relaxin-like peptide, Dilp8, or its neuronal G protein-coupled receptor, Lgr3, compromises the formation of the puparium, a sort of cocoon generated by the larva from its own external cuticle to protect itself from desiccation and predators during metamorphosis. During puparium formation, or pupariation, the cuticle is actively remodeled by stereotyped muscle contractions and then hardened enzymatically. Reduced Lgr3 receptor signaling in six ventral nerve cord interneurons leads to abnormally shaped puparia, a phenotype that can be easily, cheaply, and quickly scored by eye at the same time that it is highly informative about the integrity of the Dilp8-Lgr3 relaxin signaling pathway, the presence and integrity of the critical Lgr3-positive interneurons, and the complex behavior they mediate. Taking advantage of this, we are performing a large cell-typespecific RNAi screen in vivo using a UAS-inducible RNAi stock collection for genes expressed in the central nervous system. In this presentation we will provide an update on the ˜1500 genes screened up to now and an initial dissection of the candidate hits identified.
Fil: Zanini, Rebeca. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Heredia, Fabiana. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Ibarra, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Volonté, Yanel Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Gontijo, Alisson. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Garelli, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
European Drosophila Research Conference
Lyon
Francia
Institut de Génomique Fonctionnelle de Lyon
Laboratoire de Biologie et Modélisation de la Cellule
Ecole Normale Supérieure de Lyon
Materia
Drosophila
Relaxin
LGR3 Receptor
Genetic Screen
Pupariation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/240079
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/240079
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vivo dissection of behaviorally-relevant neuronal relaxin signaling pathwayZanini, RebecaHeredia, FabianaIbarra, JulietaVolonté, Yanel AndreaGontijo, AlissonGarelli, AndresDrosophilaRelaxinLGR3 ReceptorGenetic ScreenPupariationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Relaxin-like peptides (relaxins) belong to the insulin-like family of peptides. Instead of acting via receptor tyrosine kinases as insulin and insulin-like growth factors do, relaxins typically exert their biological effects by binding to G protein-coupled receptors. The relaxin signaling pathway is found both in invertebrates and vertebrates, including humans, and plays important roles in the reproductive, circulatory, skeletal, renal, and nervous systems. The therapeutic potential of human relaxins, for instance, are being explored due to their vasodilator, antifibrotic, and antidepressant properties. However, the biology of relaxin receptors is not fully understood. Here, our primary aim is to identify new conserved regulatory mechanisms of relaxin receptor activity. For this, we are using a relaxin-receptor-dependent Drosophila phenotype to screen for new relaxin pathway components and regulators. In Drosophila, lack of the relaxin-like peptide, Dilp8, or its neuronal G protein-coupled receptor, Lgr3, compromises the formation of the puparium, a sort of cocoon generated by the larva from its own external cuticle to protect itself from desiccation and predators during metamorphosis. During puparium formation, or pupariation, the cuticle is actively remodeled by stereotyped muscle contractions and then hardened enzymatically. Reduced Lgr3 receptor signaling in six ventral nerve cord interneurons leads to abnormally shaped puparia, a phenotype that can be easily, cheaply, and quickly scored by eye at the same time that it is highly informative about the integrity of the Dilp8-Lgr3 relaxin signaling pathway, the presence and integrity of the critical Lgr3-positive interneurons, and the complex behavior they mediate. Taking advantage of this, we are performing a large cell-typespecific RNAi screen in vivo using a UAS-inducible RNAi stock collection for genes expressed in the central nervous system. In this presentation we will provide an update on the ˜1500 genes screened up to now and an initial dissection of the candidate hits identified.Fil: Zanini, Rebeca. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; PortugalFil: Heredia, Fabiana. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; PortugalFil: Ibarra, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Volonté, Yanel Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Gontijo, Alisson. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; PortugalFil: Garelli, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaEuropean Drosophila Research ConferenceLyonFranciaInstitut de Génomique Fonctionnelle de LyonLaboratoire de Biologie et Modélisation de la CelluleEcole Normale Supérieure de LyonEuropean Drosophila Research Conference2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/240079In vivo dissection of behaviorally-relevant neuronal relaxin signaling pathway; European Drosophila Research Conference; Lyon; Francia; 2023; 430-430CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://edrclyon.sciencesconf.org/resource/page/id/34Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:35:58Zoai:ri.conicet.gov.ar:11336/240079instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:35:58.991CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vivo dissection of behaviorally-relevant neuronal relaxin signaling pathway
title In vivo dissection of behaviorally-relevant neuronal relaxin signaling pathway
spellingShingle In vivo dissection of behaviorally-relevant neuronal relaxin signaling pathway
Zanini, Rebeca
Drosophila
Relaxin
LGR3 Receptor
Genetic Screen
Pupariation
title_short In vivo dissection of behaviorally-relevant neuronal relaxin signaling pathway
title_full In vivo dissection of behaviorally-relevant neuronal relaxin signaling pathway
title_fullStr In vivo dissection of behaviorally-relevant neuronal relaxin signaling pathway
title_full_unstemmed In vivo dissection of behaviorally-relevant neuronal relaxin signaling pathway
title_sort In vivo dissection of behaviorally-relevant neuronal relaxin signaling pathway
dc.creator.none.fl_str_mv Zanini, Rebeca
Heredia, Fabiana
Ibarra, Julieta
Volonté, Yanel Andrea
Gontijo, Alisson
Garelli, Andres
author Zanini, Rebeca
author_facet Zanini, Rebeca
Heredia, Fabiana
Ibarra, Julieta
Volonté, Yanel Andrea
Gontijo, Alisson
Garelli, Andres
author_role author
author2 Heredia, Fabiana
Ibarra, Julieta
Volonté, Yanel Andrea
Gontijo, Alisson
Garelli, Andres
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Drosophila
Relaxin
LGR3 Receptor
Genetic Screen
Pupariation
topic Drosophila
Relaxin
LGR3 Receptor
Genetic Screen
Pupariation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Relaxin-like peptides (relaxins) belong to the insulin-like family of peptides. Instead of acting via receptor tyrosine kinases as insulin and insulin-like growth factors do, relaxins typically exert their biological effects by binding to G protein-coupled receptors. The relaxin signaling pathway is found both in invertebrates and vertebrates, including humans, and plays important roles in the reproductive, circulatory, skeletal, renal, and nervous systems. The therapeutic potential of human relaxins, for instance, are being explored due to their vasodilator, antifibrotic, and antidepressant properties. However, the biology of relaxin receptors is not fully understood. Here, our primary aim is to identify new conserved regulatory mechanisms of relaxin receptor activity. For this, we are using a relaxin-receptor-dependent Drosophila phenotype to screen for new relaxin pathway components and regulators. In Drosophila, lack of the relaxin-like peptide, Dilp8, or its neuronal G protein-coupled receptor, Lgr3, compromises the formation of the puparium, a sort of cocoon generated by the larva from its own external cuticle to protect itself from desiccation and predators during metamorphosis. During puparium formation, or pupariation, the cuticle is actively remodeled by stereotyped muscle contractions and then hardened enzymatically. Reduced Lgr3 receptor signaling in six ventral nerve cord interneurons leads to abnormally shaped puparia, a phenotype that can be easily, cheaply, and quickly scored by eye at the same time that it is highly informative about the integrity of the Dilp8-Lgr3 relaxin signaling pathway, the presence and integrity of the critical Lgr3-positive interneurons, and the complex behavior they mediate. Taking advantage of this, we are performing a large cell-typespecific RNAi screen in vivo using a UAS-inducible RNAi stock collection for genes expressed in the central nervous system. In this presentation we will provide an update on the ˜1500 genes screened up to now and an initial dissection of the candidate hits identified.
Fil: Zanini, Rebeca. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Heredia, Fabiana. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Ibarra, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Volonté, Yanel Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Gontijo, Alisson. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Garelli, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
European Drosophila Research Conference
Lyon
Francia
Institut de Génomique Fonctionnelle de Lyon
Laboratoire de Biologie et Modélisation de la Cellule
Ecole Normale Supérieure de Lyon
description Relaxin-like peptides (relaxins) belong to the insulin-like family of peptides. Instead of acting via receptor tyrosine kinases as insulin and insulin-like growth factors do, relaxins typically exert their biological effects by binding to G protein-coupled receptors. The relaxin signaling pathway is found both in invertebrates and vertebrates, including humans, and plays important roles in the reproductive, circulatory, skeletal, renal, and nervous systems. The therapeutic potential of human relaxins, for instance, are being explored due to their vasodilator, antifibrotic, and antidepressant properties. However, the biology of relaxin receptors is not fully understood. Here, our primary aim is to identify new conserved regulatory mechanisms of relaxin receptor activity. For this, we are using a relaxin-receptor-dependent Drosophila phenotype to screen for new relaxin pathway components and regulators. In Drosophila, lack of the relaxin-like peptide, Dilp8, or its neuronal G protein-coupled receptor, Lgr3, compromises the formation of the puparium, a sort of cocoon generated by the larva from its own external cuticle to protect itself from desiccation and predators during metamorphosis. During puparium formation, or pupariation, the cuticle is actively remodeled by stereotyped muscle contractions and then hardened enzymatically. Reduced Lgr3 receptor signaling in six ventral nerve cord interneurons leads to abnormally shaped puparia, a phenotype that can be easily, cheaply, and quickly scored by eye at the same time that it is highly informative about the integrity of the Dilp8-Lgr3 relaxin signaling pathway, the presence and integrity of the critical Lgr3-positive interneurons, and the complex behavior they mediate. Taking advantage of this, we are performing a large cell-typespecific RNAi screen in vivo using a UAS-inducible RNAi stock collection for genes expressed in the central nervous system. In this presentation we will provide an update on the ˜1500 genes screened up to now and an initial dissection of the candidate hits identified.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/240079
In vivo dissection of behaviorally-relevant neuronal relaxin signaling pathway; European Drosophila Research Conference; Lyon; Francia; 2023; 430-430
CONICET Digital
CONICET
url http://hdl.handle.net/11336/240079
identifier_str_mv In vivo dissection of behaviorally-relevant neuronal relaxin signaling pathway; European Drosophila Research Conference; Lyon; Francia; 2023; 430-430
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://edrclyon.sciencesconf.org/resource/page/id/34
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv European Drosophila Research Conference
publisher.none.fl_str_mv European Drosophila Research Conference
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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