Supramolecular analytical chemistry: Spectrofluorimetric determination of 6-hydroxymelatonin with hydroxypropyl-β-cyclodextrin

Autores
Bracamonte, Angel Guillermo; Miñambres, Guadalupe Gloria; Veglia, Alicia Viviana
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effect of the addition of native (α, β and γ) and derivative (methyl-β and hydroxypropyl-β) cyclodextrins (CD) on the fluorescence of 6-hydroxymelatonin (6HM) in water solutions was studied. The fluorescence of the substrate in the presence of hydroxypropyl-β-CD (HPCD) showed the maximum enhancement and the association constant (KA, mol-1 L) was determined (60±2) and interpreted. The fluorescence quantum yield ratio between the complex and free substrate (φ6HMCD/φ6HM) = 6.0±0.2 provides analytical advantages. On the basis of this supramolecular interaction, an alternative and sensitive spectrofluorimetric method for the determination of 6HM was developed with a detection limit of 0.71 ng mL-1. The validation of the method was performed in urine samples with very good recoveries 95-109 %.
Fil: Bracamonte, Angel Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Miñambres, Guadalupe Gloria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Veglia, Alicia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia
6-HYDROXYMELATONIN
CYCLODEXTRIN
DETERMINATION
SPECTROFLUORIMETRY
SUPRAMOLECULAR INTERACTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/61852
Acceder
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spelling Supramolecular analytical chemistry: Spectrofluorimetric determination of 6-hydroxymelatonin with hydroxypropyl-β-cyclodextrinBracamonte, Angel GuillermoMiñambres, Guadalupe GloriaVeglia, Alicia Viviana6-HYDROXYMELATONINCYCLODEXTRINDETERMINATIONSPECTROFLUORIMETRYSUPRAMOLECULAR INTERACTIONhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The effect of the addition of native (α, β and γ) and derivative (methyl-β and hydroxypropyl-β) cyclodextrins (CD) on the fluorescence of 6-hydroxymelatonin (6HM) in water solutions was studied. The fluorescence of the substrate in the presence of hydroxypropyl-β-CD (HPCD) showed the maximum enhancement and the association constant (KA, mol-1 L) was determined (60±2) and interpreted. The fluorescence quantum yield ratio between the complex and free substrate (φ6HMCD/φ6HM) = 6.0±0.2 provides analytical advantages. On the basis of this supramolecular interaction, an alternative and sensitive spectrofluorimetric method for the determination of 6HM was developed with a detection limit of 0.71 ng mL-1. The validation of the method was performed in urine samples with very good recoveries 95-109 %.Fil: Bracamonte, Angel Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Miñambres, Guadalupe Gloria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Veglia, Alicia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaArkat2011-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/61852Bracamonte, Angel Guillermo; Miñambres, Guadalupe Gloria; Veglia, Alicia Viviana; Supramolecular analytical chemistry: Spectrofluorimetric determination of 6-hydroxymelatonin with hydroxypropyl-β-cyclodextrin; Arkat; Arkivoc; 2011; 7; 5-2011; 439-44491551-70041551-7012CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://quod.lib.umich.edu/a/ark/5550190.0012.736/1info:eu-repo/semantics/altIdentifier/doi/10.3998/ark.5550190.0012.736info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:38:55Zoai:ri.conicet.gov.ar:11336/61852instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:38:55.871CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Supramolecular analytical chemistry: Spectrofluorimetric determination of 6-hydroxymelatonin with hydroxypropyl-β-cyclodextrin
title Supramolecular analytical chemistry: Spectrofluorimetric determination of 6-hydroxymelatonin with hydroxypropyl-β-cyclodextrin
spellingShingle Supramolecular analytical chemistry: Spectrofluorimetric determination of 6-hydroxymelatonin with hydroxypropyl-β-cyclodextrin
Bracamonte, Angel Guillermo
6-HYDROXYMELATONIN
CYCLODEXTRIN
DETERMINATION
SPECTROFLUORIMETRY
SUPRAMOLECULAR INTERACTION
title_short Supramolecular analytical chemistry: Spectrofluorimetric determination of 6-hydroxymelatonin with hydroxypropyl-β-cyclodextrin
title_full Supramolecular analytical chemistry: Spectrofluorimetric determination of 6-hydroxymelatonin with hydroxypropyl-β-cyclodextrin
title_fullStr Supramolecular analytical chemistry: Spectrofluorimetric determination of 6-hydroxymelatonin with hydroxypropyl-β-cyclodextrin
title_full_unstemmed Supramolecular analytical chemistry: Spectrofluorimetric determination of 6-hydroxymelatonin with hydroxypropyl-β-cyclodextrin
title_sort Supramolecular analytical chemistry: Spectrofluorimetric determination of 6-hydroxymelatonin with hydroxypropyl-β-cyclodextrin
dc.creator.none.fl_str_mv Bracamonte, Angel Guillermo
Miñambres, Guadalupe Gloria
Veglia, Alicia Viviana
author Bracamonte, Angel Guillermo
author_facet Bracamonte, Angel Guillermo
Miñambres, Guadalupe Gloria
Veglia, Alicia Viviana
author_role author
author2 Miñambres, Guadalupe Gloria
Veglia, Alicia Viviana
author2_role author
author
dc.subject.none.fl_str_mv 6-HYDROXYMELATONIN
CYCLODEXTRIN
DETERMINATION
SPECTROFLUORIMETRY
SUPRAMOLECULAR INTERACTION
topic 6-HYDROXYMELATONIN
CYCLODEXTRIN
DETERMINATION
SPECTROFLUORIMETRY
SUPRAMOLECULAR INTERACTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The effect of the addition of native (α, β and γ) and derivative (methyl-β and hydroxypropyl-β) cyclodextrins (CD) on the fluorescence of 6-hydroxymelatonin (6HM) in water solutions was studied. The fluorescence of the substrate in the presence of hydroxypropyl-β-CD (HPCD) showed the maximum enhancement and the association constant (KA, mol-1 L) was determined (60±2) and interpreted. The fluorescence quantum yield ratio between the complex and free substrate (φ6HMCD/φ6HM) = 6.0±0.2 provides analytical advantages. On the basis of this supramolecular interaction, an alternative and sensitive spectrofluorimetric method for the determination of 6HM was developed with a detection limit of 0.71 ng mL-1. The validation of the method was performed in urine samples with very good recoveries 95-109 %.
Fil: Bracamonte, Angel Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Miñambres, Guadalupe Gloria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Veglia, Alicia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description The effect of the addition of native (α, β and γ) and derivative (methyl-β and hydroxypropyl-β) cyclodextrins (CD) on the fluorescence of 6-hydroxymelatonin (6HM) in water solutions was studied. The fluorescence of the substrate in the presence of hydroxypropyl-β-CD (HPCD) showed the maximum enhancement and the association constant (KA, mol-1 L) was determined (60±2) and interpreted. The fluorescence quantum yield ratio between the complex and free substrate (φ6HMCD/φ6HM) = 6.0±0.2 provides analytical advantages. On the basis of this supramolecular interaction, an alternative and sensitive spectrofluorimetric method for the determination of 6HM was developed with a detection limit of 0.71 ng mL-1. The validation of the method was performed in urine samples with very good recoveries 95-109 %.
publishDate 2011
dc.date.none.fl_str_mv 2011-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/61852
Bracamonte, Angel Guillermo; Miñambres, Guadalupe Gloria; Veglia, Alicia Viviana; Supramolecular analytical chemistry: Spectrofluorimetric determination of 6-hydroxymelatonin with hydroxypropyl-β-cyclodextrin; Arkat; Arkivoc; 2011; 7; 5-2011; 439-4449
1551-7004
1551-7012
CONICET Digital
CONICET
url http://hdl.handle.net/11336/61852
identifier_str_mv Bracamonte, Angel Guillermo; Miñambres, Guadalupe Gloria; Veglia, Alicia Viviana; Supramolecular analytical chemistry: Spectrofluorimetric determination of 6-hydroxymelatonin with hydroxypropyl-β-cyclodextrin; Arkat; Arkivoc; 2011; 7; 5-2011; 439-4449
1551-7004
1551-7012
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://quod.lib.umich.edu/a/ark/5550190.0012.736/1
info:eu-repo/semantics/altIdentifier/doi/10.3998/ark.5550190.0012.736
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Arkat
publisher.none.fl_str_mv Arkat
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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