Impact of supramolecular interactions of dextran-β-cyclodextrin polymers on invertase activity in freeze-dried systems

Autores
Santagapita, Patricio Roman; Mazzobre, Maria Florencia; Buera, Maria del Pilar; Ramírez, Héctor L.; Gómez Brizuela, Leissy; Corti, Horacio Roberto; Villalonga Santana, Reynaldo
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
β-cyclodextrin-grafted dextrans with spacer arms of different length were employed to evaluate the impact of supramolecular interactions on invertase activity. The modified dextrans were used as single additives or combined with trehalose in freeze-dried formulations containing invertase. Enzyme activity conservation was analyzed after freeze-drying and thermal treatment. The change of glass transition temperatures (Tg) was also evaluated and related to effective interactions. Outstanding differences on enzyme stability were mainly related to the effect of the spacer arm length on polymer-enzyme interactions, since both the degree of substitution and the molecular weight were similar for the two polymers. This change of effective interactions was also manifested in the pronounced reduction of Tg values, and were related to the chemical modification of the backbone during oxidation, and to the attachment of the β-cyclodextrin units with spacer arms of different length on dextran.
Fil: Santagapita, Patricio Roman. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mazzobre, Maria Florencia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Buera, Maria del Pilar. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ramírez, Héctor L.. Universidad de Matanzas Camilo Cienfuegos.; Cuba
Fil: Gómez Brizuela, Leissy. Universidad de Matanzas Camilo Cienfuegos.; Cuba
Fil: Corti, Horacio Roberto. Comisión Nacional de Energía Atómica. Gerencia de Área Investigaciones y Aplicaciones No Nucleares. Gerencia Física (CAC). Departamento de Física de la Materia Condensada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Villalonga Santana, Reynaldo. Universidad Complutense de Madrid; España
Materia
supramolecular interactions
dextran
beta-cyclodextrin
glass transition temperature (Tg)
enzyme stability
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/113122
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/113122
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Impact of supramolecular interactions of dextran-β-cyclodextrin polymers on invertase activity in freeze-dried systemsSantagapita, Patricio RomanMazzobre, Maria FlorenciaBuera, Maria del PilarRamírez, Héctor L.Gómez Brizuela, LeissyCorti, Horacio RobertoVillalonga Santana, Reynaldosupramolecular interactionsdextranbeta-cyclodextringlass transition temperature (Tg)enzyme stabilityhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1β-cyclodextrin-grafted dextrans with spacer arms of different length were employed to evaluate the impact of supramolecular interactions on invertase activity. The modified dextrans were used as single additives or combined with trehalose in freeze-dried formulations containing invertase. Enzyme activity conservation was analyzed after freeze-drying and thermal treatment. The change of glass transition temperatures (Tg) was also evaluated and related to effective interactions. Outstanding differences on enzyme stability were mainly related to the effect of the spacer arm length on polymer-enzyme interactions, since both the degree of substitution and the molecular weight were similar for the two polymers. This change of effective interactions was also manifested in the pronounced reduction of Tg values, and were related to the chemical modification of the backbone during oxidation, and to the attachment of the β-cyclodextrin units with spacer arms of different length on dextran.Fil: Santagapita, Patricio Roman. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mazzobre, Maria Florencia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Buera, Maria del Pilar. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ramírez, Héctor L.. Universidad de Matanzas Camilo Cienfuegos.; CubaFil: Gómez Brizuela, Leissy. Universidad de Matanzas Camilo Cienfuegos.; CubaFil: Corti, Horacio Roberto. Comisión Nacional de Energía Atómica. Gerencia de Área Investigaciones y Aplicaciones No Nucleares. Gerencia Física (CAC). Departamento de Física de la Materia Condensada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Villalonga Santana, Reynaldo. Universidad Complutense de Madrid; EspañaAmerican Chemical Society2015-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/113122Santagapita, Patricio Roman; Mazzobre, Maria Florencia; Buera, Maria del Pilar; Ramírez, Héctor L.; Gómez Brizuela, Leissy; et al.; Impact of supramolecular interactions of dextran-β-cyclodextrin polymers on invertase activity in freeze-dried systems; American Chemical Society; Biotechnology Progress; 31; 3; 5-2015; 791-7988756-7938CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/btpr.2067info:eu-repo/semantics/altIdentifier/url/https://aiche.onlinelibrary.wiley.com/doi/abs/10.1002/btpr.2067info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:00:10Zoai:ri.conicet.gov.ar:11336/113122instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:00:11.011CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Impact of supramolecular interactions of dextran-β-cyclodextrin polymers on invertase activity in freeze-dried systems
title Impact of supramolecular interactions of dextran-β-cyclodextrin polymers on invertase activity in freeze-dried systems
spellingShingle Impact of supramolecular interactions of dextran-β-cyclodextrin polymers on invertase activity in freeze-dried systems
Santagapita, Patricio Roman
supramolecular interactions
dextran
beta-cyclodextrin
glass transition temperature (Tg)
enzyme stability
title_short Impact of supramolecular interactions of dextran-β-cyclodextrin polymers on invertase activity in freeze-dried systems
title_full Impact of supramolecular interactions of dextran-β-cyclodextrin polymers on invertase activity in freeze-dried systems
title_fullStr Impact of supramolecular interactions of dextran-β-cyclodextrin polymers on invertase activity in freeze-dried systems
title_full_unstemmed Impact of supramolecular interactions of dextran-β-cyclodextrin polymers on invertase activity in freeze-dried systems
title_sort Impact of supramolecular interactions of dextran-β-cyclodextrin polymers on invertase activity in freeze-dried systems
dc.creator.none.fl_str_mv Santagapita, Patricio Roman
Mazzobre, Maria Florencia
Buera, Maria del Pilar
Ramírez, Héctor L.
Gómez Brizuela, Leissy
Corti, Horacio Roberto
Villalonga Santana, Reynaldo
author Santagapita, Patricio Roman
author_facet Santagapita, Patricio Roman
Mazzobre, Maria Florencia
Buera, Maria del Pilar
Ramírez, Héctor L.
Gómez Brizuela, Leissy
Corti, Horacio Roberto
Villalonga Santana, Reynaldo
author_role author
author2 Mazzobre, Maria Florencia
Buera, Maria del Pilar
Ramírez, Héctor L.
Gómez Brizuela, Leissy
Corti, Horacio Roberto
Villalonga Santana, Reynaldo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv supramolecular interactions
dextran
beta-cyclodextrin
glass transition temperature (Tg)
enzyme stability
topic supramolecular interactions
dextran
beta-cyclodextrin
glass transition temperature (Tg)
enzyme stability
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv β-cyclodextrin-grafted dextrans with spacer arms of different length were employed to evaluate the impact of supramolecular interactions on invertase activity. The modified dextrans were used as single additives or combined with trehalose in freeze-dried formulations containing invertase. Enzyme activity conservation was analyzed after freeze-drying and thermal treatment. The change of glass transition temperatures (Tg) was also evaluated and related to effective interactions. Outstanding differences on enzyme stability were mainly related to the effect of the spacer arm length on polymer-enzyme interactions, since both the degree of substitution and the molecular weight were similar for the two polymers. This change of effective interactions was also manifested in the pronounced reduction of Tg values, and were related to the chemical modification of the backbone during oxidation, and to the attachment of the β-cyclodextrin units with spacer arms of different length on dextran.
Fil: Santagapita, Patricio Roman. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mazzobre, Maria Florencia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Buera, Maria del Pilar. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ramírez, Héctor L.. Universidad de Matanzas Camilo Cienfuegos.; Cuba
Fil: Gómez Brizuela, Leissy. Universidad de Matanzas Camilo Cienfuegos.; Cuba
Fil: Corti, Horacio Roberto. Comisión Nacional de Energía Atómica. Gerencia de Área Investigaciones y Aplicaciones No Nucleares. Gerencia Física (CAC). Departamento de Física de la Materia Condensada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Villalonga Santana, Reynaldo. Universidad Complutense de Madrid; España
description β-cyclodextrin-grafted dextrans with spacer arms of different length were employed to evaluate the impact of supramolecular interactions on invertase activity. The modified dextrans were used as single additives or combined with trehalose in freeze-dried formulations containing invertase. Enzyme activity conservation was analyzed after freeze-drying and thermal treatment. The change of glass transition temperatures (Tg) was also evaluated and related to effective interactions. Outstanding differences on enzyme stability were mainly related to the effect of the spacer arm length on polymer-enzyme interactions, since both the degree of substitution and the molecular weight were similar for the two polymers. This change of effective interactions was also manifested in the pronounced reduction of Tg values, and were related to the chemical modification of the backbone during oxidation, and to the attachment of the β-cyclodextrin units with spacer arms of different length on dextran.
publishDate 2015
dc.date.none.fl_str_mv 2015-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/113122
Santagapita, Patricio Roman; Mazzobre, Maria Florencia; Buera, Maria del Pilar; Ramírez, Héctor L.; Gómez Brizuela, Leissy; et al.; Impact of supramolecular interactions of dextran-β-cyclodextrin polymers on invertase activity in freeze-dried systems; American Chemical Society; Biotechnology Progress; 31; 3; 5-2015; 791-798
8756-7938
CONICET Digital
CONICET
url http://hdl.handle.net/11336/113122
identifier_str_mv Santagapita, Patricio Roman; Mazzobre, Maria Florencia; Buera, Maria del Pilar; Ramírez, Héctor L.; Gómez Brizuela, Leissy; et al.; Impact of supramolecular interactions of dextran-β-cyclodextrin polymers on invertase activity in freeze-dried systems; American Chemical Society; Biotechnology Progress; 31; 3; 5-2015; 791-798
8756-7938
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/btpr.2067
info:eu-repo/semantics/altIdentifier/url/https://aiche.onlinelibrary.wiley.com/doi/abs/10.1002/btpr.2067
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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