Molecular identification of a cyclodextrin glycosyltransferase-producinmicroorganism and phylogenetic assessment of enzymatic activities

Autores
Caminata Landriel, Soledad; Castillo, Julieta de Las Mercedes; Taboga, Oscar Alberto; Ferrarotti, Susana Alicia; Gottlieb, Alexandra Marina; Costa, Hernán
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cyclodextrin glycosyltransferases (CGTases) are important enzymes in the biotechnology field because they catalyze starch conversion into cyclodextrins and linear oligosaccharides, which are used in food, pharmaceutical and cosmetic industries. The CGTases are classified according to their product specificity in α-, β-, α/β-and γ-CGTases. As molecular markers are the preferred tool for bacterial identification, we employed six molecular markers (16S rRNA, dnaK, gyrB, recA, rpoB and tufA) to test the identification of a CGTase-producing bacterial strain (DF 9R) in a phylogenetic context. In addition, we assessed the phylogenetic relationship of CGTases along bacterial evolution. The results obtained here allowed us to identify the strain DF 9R as Paenibacillus barengoltzii, and to unveil a complex origin for CGTase types during archaeal and bacterial evolution. We postulate that the α-CGTase activity represents the ancestral type, and that the γ-activity may have derived from β-CGTases.
Fil: Caminata Landriel, Soledad. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; Argentina
Fil: Castillo de las Mercedes Julieta. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; Argentina
Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Ferrarotti, Susana Alicia. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; Argentina
Fil: Gottlieb, Alexandra Marina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución. Laboratorio de Citogenética y Evolución; Argentina
Fil: Costa, Hernán. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; Argentina. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina
Materia
16S RRNA
CYCLODEXTRIN GLYCOSYLTRANSFERASES
HOUSEKEEPING GENES
PAENIBACILLUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/93882
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular identification of a cyclodextrin glycosyltransferase-producinmicroorganism and phylogenetic assessment of enzymatic activitiesCaminata Landriel, SoledadCastillo, Julieta de Las MercedesTaboga, Oscar AlbertoFerrarotti, Susana AliciaGottlieb, Alexandra MarinaCosta, Hernán16S RRNACYCLODEXTRIN GLYCOSYLTRANSFERASESHOUSEKEEPING GENESPAENIBACILLUShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cyclodextrin glycosyltransferases (CGTases) are important enzymes in the biotechnology field because they catalyze starch conversion into cyclodextrins and linear oligosaccharides, which are used in food, pharmaceutical and cosmetic industries. The CGTases are classified according to their product specificity in α-, β-, α/β-and γ-CGTases. As molecular markers are the preferred tool for bacterial identification, we employed six molecular markers (16S rRNA, dnaK, gyrB, recA, rpoB and tufA) to test the identification of a CGTase-producing bacterial strain (DF 9R) in a phylogenetic context. In addition, we assessed the phylogenetic relationship of CGTases along bacterial evolution. The results obtained here allowed us to identify the strain DF 9R as Paenibacillus barengoltzii, and to unveil a complex origin for CGTase types during archaeal and bacterial evolution. We postulate that the α-CGTase activity represents the ancestral type, and that the γ-activity may have derived from β-CGTases.Fil: Caminata Landriel, Soledad. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; ArgentinaFil: Castillo de las Mercedes Julieta. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; ArgentinaFil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Ferrarotti, Susana Alicia. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; ArgentinaFil: Gottlieb, Alexandra Marina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución. Laboratorio de Citogenética y Evolución; ArgentinaFil: Costa, Hernán. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; Argentina. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; ArgentinaAcademia Brasileira de Ciencias2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/93882Caminata Landriel, Soledad; Castillo, Julieta de Las Mercedes; Taboga, Oscar Alberto; Ferrarotti, Susana Alicia; Gottlieb, Alexandra Marina; et al.; Molecular identification of a cyclodextrin glycosyltransferase-producinmicroorganism and phylogenetic assessment of enzymatic activities; Academia Brasileira de Ciencias; Anais da Academia Brasileira de Ciencias; 91; 3; 12-2018; 1-11; e201805680001-37651678-2690CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1590/0001-3765201920180568info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/tnpyjyinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:36:59Zoai:ri.conicet.gov.ar:11336/93882instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:36:59.915CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular identification of a cyclodextrin glycosyltransferase-producinmicroorganism and phylogenetic assessment of enzymatic activities
title Molecular identification of a cyclodextrin glycosyltransferase-producinmicroorganism and phylogenetic assessment of enzymatic activities
spellingShingle Molecular identification of a cyclodextrin glycosyltransferase-producinmicroorganism and phylogenetic assessment of enzymatic activities
Caminata Landriel, Soledad
16S RRNA
CYCLODEXTRIN GLYCOSYLTRANSFERASES
HOUSEKEEPING GENES
PAENIBACILLUS
title_short Molecular identification of a cyclodextrin glycosyltransferase-producinmicroorganism and phylogenetic assessment of enzymatic activities
title_full Molecular identification of a cyclodextrin glycosyltransferase-producinmicroorganism and phylogenetic assessment of enzymatic activities
title_fullStr Molecular identification of a cyclodextrin glycosyltransferase-producinmicroorganism and phylogenetic assessment of enzymatic activities
title_full_unstemmed Molecular identification of a cyclodextrin glycosyltransferase-producinmicroorganism and phylogenetic assessment of enzymatic activities
title_sort Molecular identification of a cyclodextrin glycosyltransferase-producinmicroorganism and phylogenetic assessment of enzymatic activities
dc.creator.none.fl_str_mv Caminata Landriel, Soledad
Castillo, Julieta de Las Mercedes
Taboga, Oscar Alberto
Ferrarotti, Susana Alicia
Gottlieb, Alexandra Marina
Costa, Hernán
author Caminata Landriel, Soledad
author_facet Caminata Landriel, Soledad
Castillo, Julieta de Las Mercedes
Taboga, Oscar Alberto
Ferrarotti, Susana Alicia
Gottlieb, Alexandra Marina
Costa, Hernán
author_role author
author2 Castillo, Julieta de Las Mercedes
Taboga, Oscar Alberto
Ferrarotti, Susana Alicia
Gottlieb, Alexandra Marina
Costa, Hernán
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv 16S RRNA
CYCLODEXTRIN GLYCOSYLTRANSFERASES
HOUSEKEEPING GENES
PAENIBACILLUS
topic 16S RRNA
CYCLODEXTRIN GLYCOSYLTRANSFERASES
HOUSEKEEPING GENES
PAENIBACILLUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cyclodextrin glycosyltransferases (CGTases) are important enzymes in the biotechnology field because they catalyze starch conversion into cyclodextrins and linear oligosaccharides, which are used in food, pharmaceutical and cosmetic industries. The CGTases are classified according to their product specificity in α-, β-, α/β-and γ-CGTases. As molecular markers are the preferred tool for bacterial identification, we employed six molecular markers (16S rRNA, dnaK, gyrB, recA, rpoB and tufA) to test the identification of a CGTase-producing bacterial strain (DF 9R) in a phylogenetic context. In addition, we assessed the phylogenetic relationship of CGTases along bacterial evolution. The results obtained here allowed us to identify the strain DF 9R as Paenibacillus barengoltzii, and to unveil a complex origin for CGTase types during archaeal and bacterial evolution. We postulate that the α-CGTase activity represents the ancestral type, and that the γ-activity may have derived from β-CGTases.
Fil: Caminata Landriel, Soledad. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; Argentina
Fil: Castillo de las Mercedes Julieta. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; Argentina
Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Ferrarotti, Susana Alicia. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; Argentina
Fil: Gottlieb, Alexandra Marina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución. Laboratorio de Citogenética y Evolución; Argentina
Fil: Costa, Hernán. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; Argentina. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina
description Cyclodextrin glycosyltransferases (CGTases) are important enzymes in the biotechnology field because they catalyze starch conversion into cyclodextrins and linear oligosaccharides, which are used in food, pharmaceutical and cosmetic industries. The CGTases are classified according to their product specificity in α-, β-, α/β-and γ-CGTases. As molecular markers are the preferred tool for bacterial identification, we employed six molecular markers (16S rRNA, dnaK, gyrB, recA, rpoB and tufA) to test the identification of a CGTase-producing bacterial strain (DF 9R) in a phylogenetic context. In addition, we assessed the phylogenetic relationship of CGTases along bacterial evolution. The results obtained here allowed us to identify the strain DF 9R as Paenibacillus barengoltzii, and to unveil a complex origin for CGTase types during archaeal and bacterial evolution. We postulate that the α-CGTase activity represents the ancestral type, and that the γ-activity may have derived from β-CGTases.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/93882
Caminata Landriel, Soledad; Castillo, Julieta de Las Mercedes; Taboga, Oscar Alberto; Ferrarotti, Susana Alicia; Gottlieb, Alexandra Marina; et al.; Molecular identification of a cyclodextrin glycosyltransferase-producinmicroorganism and phylogenetic assessment of enzymatic activities; Academia Brasileira de Ciencias; Anais da Academia Brasileira de Ciencias; 91; 3; 12-2018; 1-11; e20180568
0001-3765
1678-2690
CONICET Digital
CONICET
url http://hdl.handle.net/11336/93882
identifier_str_mv Caminata Landriel, Soledad; Castillo, Julieta de Las Mercedes; Taboga, Oscar Alberto; Ferrarotti, Susana Alicia; Gottlieb, Alexandra Marina; et al.; Molecular identification of a cyclodextrin glycosyltransferase-producinmicroorganism and phylogenetic assessment of enzymatic activities; Academia Brasileira de Ciencias; Anais da Academia Brasileira de Ciencias; 91; 3; 12-2018; 1-11; e20180568
0001-3765
1678-2690
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1590/0001-3765201920180568
info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/tnpyjy
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academia Brasileira de Ciencias
publisher.none.fl_str_mv Academia Brasileira de Ciencias
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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