Molecular identification of a cyclodextrin glycosyltransferase-producing microorganism and phylogenetic assessment of enzymatic activities

Autores
Caminata Landriel, Soledad; Castillo, Julieta D. L. M.; Taboga, Oscar Alberto; Ferrarotti, Susana Alicia; Gottlieb, Alexandra Marina; Costa, Hernán
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cyclodextrin glycosyltransferases (CGTases) are important enzymes in the biotechnology field because they catalyze starch conversion into cyclodextrins and linear oligosaccharides, which are used in food, pharmaceutical and cosmetic industries. The CGTases are classified according to their product specificity in α-, β-, α/β- and γ-CGTases. As molecular markers are the preferred tool for bacterial identification, we employed six molecular markers (16S rRNA, dnaK, gyrB, recA, rpoB and tufA) to test the identification of a CGTase-producing bacterial strain (DF 9R) in a phylogenetic context. In addition, we assessed the phylogenetic relationship of CGTases along bacterial evolution. The results obtained here allowed us to identify the strain DF 9R as Paenibacillus barengoltzii, and to unveil a complex origin for CGTase types during archaeal and bacterial evolution. We postulate that the α-CGTase activity represents the ancestral type, and that the γ-activity may have derived from β-CGTases.
Instituto de Biotecnología
Fil: Caminata Landriel, Soledad. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Química Biológica; Argentina
Fil: Castillo, Julieta D. L. M. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Química Biológica; Argentina
Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ferrarotti, Susana Alicia. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Química Biológica; Argentina.
Fil: Gottlieb, Alexandra Marina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Costa, Hernán. Universidad Nacional de Luján. Departamento de Ciencias Básicas.Laboratorio de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Anais da Academia Brasileira de Ciências 91 (3) : e20180568. (Octubre 2019)
Materia
Paenibacillus
Glicosidasas
Marcadores Genéticos
Actividad Enzimática
Filogenia
Glycosidases
Genetic Markers
Enzyme Activity
Phylogeny
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/6671
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oai_identifier_str oai:localhost:20.500.12123/6671
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network_name_str INTA Digital (INTA)
spelling Molecular identification of a cyclodextrin glycosyltransferase-producing microorganism and phylogenetic assessment of enzymatic activitiesCaminata Landriel, SoledadCastillo, Julieta D. L. M.Taboga, Oscar AlbertoFerrarotti, Susana AliciaGottlieb, Alexandra MarinaCosta, HernánPaenibacillusGlicosidasasMarcadores GenéticosActividad EnzimáticaFilogeniaGlycosidasesGenetic MarkersEnzyme ActivityPhylogenyCyclodextrin glycosyltransferases (CGTases) are important enzymes in the biotechnology field because they catalyze starch conversion into cyclodextrins and linear oligosaccharides, which are used in food, pharmaceutical and cosmetic industries. The CGTases are classified according to their product specificity in α-, β-, α/β- and γ-CGTases. As molecular markers are the preferred tool for bacterial identification, we employed six molecular markers (16S rRNA, dnaK, gyrB, recA, rpoB and tufA) to test the identification of a CGTase-producing bacterial strain (DF 9R) in a phylogenetic context. In addition, we assessed the phylogenetic relationship of CGTases along bacterial evolution. The results obtained here allowed us to identify the strain DF 9R as Paenibacillus barengoltzii, and to unveil a complex origin for CGTase types during archaeal and bacterial evolution. We postulate that the α-CGTase activity represents the ancestral type, and that the γ-activity may have derived from β-CGTases.Instituto de BiotecnologíaFil: Caminata Landriel, Soledad. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Química Biológica; ArgentinaFil: Castillo, Julieta D. L. M. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Química Biológica; ArgentinaFil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ferrarotti, Susana Alicia. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Química Biológica; Argentina.Fil: Gottlieb, Alexandra Marina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Costa, Hernán. Universidad Nacional de Luján. Departamento de Ciencias Básicas.Laboratorio de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaAcademia Brasileira de Ciências2020-01-14T13:30:43Z2020-01-14T13:30:43Z2019-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/6671http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500620&lng=en&nrm=iso&tlng=en1678-2690http://dx.doi.org/10.1590/0001-3765201920180568Anais da Academia Brasileira de Ciências 91 (3) : e20180568. (Octubre 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:52Zoai:localhost:20.500.12123/6671instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:52.368INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Molecular identification of a cyclodextrin glycosyltransferase-producing microorganism and phylogenetic assessment of enzymatic activities
title Molecular identification of a cyclodextrin glycosyltransferase-producing microorganism and phylogenetic assessment of enzymatic activities
spellingShingle Molecular identification of a cyclodextrin glycosyltransferase-producing microorganism and phylogenetic assessment of enzymatic activities
Caminata Landriel, Soledad
Paenibacillus
Glicosidasas
Marcadores Genéticos
Actividad Enzimática
Filogenia
Glycosidases
Genetic Markers
Enzyme Activity
Phylogeny
title_short Molecular identification of a cyclodextrin glycosyltransferase-producing microorganism and phylogenetic assessment of enzymatic activities
title_full Molecular identification of a cyclodextrin glycosyltransferase-producing microorganism and phylogenetic assessment of enzymatic activities
title_fullStr Molecular identification of a cyclodextrin glycosyltransferase-producing microorganism and phylogenetic assessment of enzymatic activities
title_full_unstemmed Molecular identification of a cyclodextrin glycosyltransferase-producing microorganism and phylogenetic assessment of enzymatic activities
title_sort Molecular identification of a cyclodextrin glycosyltransferase-producing microorganism and phylogenetic assessment of enzymatic activities
dc.creator.none.fl_str_mv Caminata Landriel, Soledad
Castillo, Julieta D. L. M.
Taboga, Oscar Alberto
Ferrarotti, Susana Alicia
Gottlieb, Alexandra Marina
Costa, Hernán
author Caminata Landriel, Soledad
author_facet Caminata Landriel, Soledad
Castillo, Julieta D. L. M.
Taboga, Oscar Alberto
Ferrarotti, Susana Alicia
Gottlieb, Alexandra Marina
Costa, Hernán
author_role author
author2 Castillo, Julieta D. L. M.
Taboga, Oscar Alberto
Ferrarotti, Susana Alicia
Gottlieb, Alexandra Marina
Costa, Hernán
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Paenibacillus
Glicosidasas
Marcadores Genéticos
Actividad Enzimática
Filogenia
Glycosidases
Genetic Markers
Enzyme Activity
Phylogeny
topic Paenibacillus
Glicosidasas
Marcadores Genéticos
Actividad Enzimática
Filogenia
Glycosidases
Genetic Markers
Enzyme Activity
Phylogeny
dc.description.none.fl_txt_mv Cyclodextrin glycosyltransferases (CGTases) are important enzymes in the biotechnology field because they catalyze starch conversion into cyclodextrins and linear oligosaccharides, which are used in food, pharmaceutical and cosmetic industries. The CGTases are classified according to their product specificity in α-, β-, α/β- and γ-CGTases. As molecular markers are the preferred tool for bacterial identification, we employed six molecular markers (16S rRNA, dnaK, gyrB, recA, rpoB and tufA) to test the identification of a CGTase-producing bacterial strain (DF 9R) in a phylogenetic context. In addition, we assessed the phylogenetic relationship of CGTases along bacterial evolution. The results obtained here allowed us to identify the strain DF 9R as Paenibacillus barengoltzii, and to unveil a complex origin for CGTase types during archaeal and bacterial evolution. We postulate that the α-CGTase activity represents the ancestral type, and that the γ-activity may have derived from β-CGTases.
Instituto de Biotecnología
Fil: Caminata Landriel, Soledad. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Química Biológica; Argentina
Fil: Castillo, Julieta D. L. M. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Química Biológica; Argentina
Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ferrarotti, Susana Alicia. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Química Biológica; Argentina.
Fil: Gottlieb, Alexandra Marina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Costa, Hernán. Universidad Nacional de Luján. Departamento de Ciencias Básicas.Laboratorio de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Cyclodextrin glycosyltransferases (CGTases) are important enzymes in the biotechnology field because they catalyze starch conversion into cyclodextrins and linear oligosaccharides, which are used in food, pharmaceutical and cosmetic industries. The CGTases are classified according to their product specificity in α-, β-, α/β- and γ-CGTases. As molecular markers are the preferred tool for bacterial identification, we employed six molecular markers (16S rRNA, dnaK, gyrB, recA, rpoB and tufA) to test the identification of a CGTase-producing bacterial strain (DF 9R) in a phylogenetic context. In addition, we assessed the phylogenetic relationship of CGTases along bacterial evolution. The results obtained here allowed us to identify the strain DF 9R as Paenibacillus barengoltzii, and to unveil a complex origin for CGTase types during archaeal and bacterial evolution. We postulate that the α-CGTase activity represents the ancestral type, and that the γ-activity may have derived from β-CGTases.
publishDate 2019
dc.date.none.fl_str_mv 2019-10
2020-01-14T13:30:43Z
2020-01-14T13:30:43Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/6671
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500620&lng=en&nrm=iso&tlng=en
1678-2690
http://dx.doi.org/10.1590/0001-3765201920180568
url http://hdl.handle.net/20.500.12123/6671
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500620&lng=en&nrm=iso&tlng=en
http://dx.doi.org/10.1590/0001-3765201920180568
identifier_str_mv 1678-2690
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências 91 (3) : e20180568. (Octubre 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.749369

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