Continuous production of cyclodextrins in an ultrafiltration membrane reactor, catalyzed by Cyclodextrin glycosyltransferase from Bacillus circulans DF 9R

Autores
Rodríguez Gastón, Jorgelina Andrea; Costa, Hernán; Ferrarotti, Susana Alicia
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cyclodextrins (CDs) are cyclic oligosaccharides of wide industrial application, whose synthesis is catalyzed by Cyclodextrin glycosyltransferase (CGTase) from starch. Here, CDs were produced using CGTase from Bacillus circulans DF 9R in continuous process and an ultrafiltration membrane reactor. The batch process was conducted as a control. This method allowed increasing the yield from 40% to 55.6% and the productivity from 26.1 to 99.5 mg of CD per unit of enzyme. The method also allowed obtaining a high purity product. The flow rate remained at 50% of its initial value after 24 hours of process, improving the results described in the literature for starch hydrolysis processes. CGTase remained active throughout the process, which could be explained by the protective effect of the substrate and reaction products on CGTase stability. In addition, batch processes were developed using starches from different sources. We concluded that any of the starches studied could be used as substrate for CD production with similar yields and product specificity.
Fil: Rodríguez Gastón, Jorgelina Andrea. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; Argentina
Fil: Costa, Hernán. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ferrarotti, Susana Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; Argentina
Materia
CYCLODEXTRINS
CYCLODEXTRIN GLYCOSYLTRANSFERASE
BACILLUS CIRCULANS
STARCH CONVERSION
ULTRAFILTRATION MEMBRANE REACTOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/109312
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/109312
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Continuous production of cyclodextrins in an ultrafiltration membrane reactor, catalyzed by Cyclodextrin glycosyltransferase from Bacillus circulans DF 9RRodríguez Gastón, Jorgelina AndreaCosta, HernánFerrarotti, Susana AliciaCYCLODEXTRINSCYCLODEXTRIN GLYCOSYLTRANSFERASEBACILLUS CIRCULANSSTARCH CONVERSIONULTRAFILTRATION MEMBRANE REACTORhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Cyclodextrins (CDs) are cyclic oligosaccharides of wide industrial application, whose synthesis is catalyzed by Cyclodextrin glycosyltransferase (CGTase) from starch. Here, CDs were produced using CGTase from Bacillus circulans DF 9R in continuous process and an ultrafiltration membrane reactor. The batch process was conducted as a control. This method allowed increasing the yield from 40% to 55.6% and the productivity from 26.1 to 99.5 mg of CD per unit of enzyme. The method also allowed obtaining a high purity product. The flow rate remained at 50% of its initial value after 24 hours of process, improving the results described in the literature for starch hydrolysis processes. CGTase remained active throughout the process, which could be explained by the protective effect of the substrate and reaction products on CGTase stability. In addition, batch processes were developed using starches from different sources. We concluded that any of the starches studied could be used as substrate for CD production with similar yields and product specificity.Fil: Rodríguez Gastón, Jorgelina Andrea. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; ArgentinaFil: Costa, Hernán. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ferrarotti, Susana Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; ArgentinaAmerican Chemical Society2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/109312Rodríguez Gastón, Jorgelina Andrea; Costa, Hernán; Ferrarotti, Susana Alicia; Continuous production of cyclodextrins in an ultrafiltration membrane reactor, catalyzed by Cyclodextrin glycosyltransferase from Bacillus circulans DF 9R; American Chemical Society; Biotechnology Progress; 31; 3; 1-2015; 695-6998756-7938CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/BTPR.2053info:eu-repo/semantics/altIdentifier/url/https://aiche.onlinelibrary.wiley.com/doi/abs/10.1002/btpr.2053info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:02:35Zoai:ri.conicet.gov.ar:11336/109312instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:02:35.805CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Continuous production of cyclodextrins in an ultrafiltration membrane reactor, catalyzed by Cyclodextrin glycosyltransferase from Bacillus circulans DF 9R
title Continuous production of cyclodextrins in an ultrafiltration membrane reactor, catalyzed by Cyclodextrin glycosyltransferase from Bacillus circulans DF 9R
spellingShingle Continuous production of cyclodextrins in an ultrafiltration membrane reactor, catalyzed by Cyclodextrin glycosyltransferase from Bacillus circulans DF 9R
Rodríguez Gastón, Jorgelina Andrea
CYCLODEXTRINS
CYCLODEXTRIN GLYCOSYLTRANSFERASE
BACILLUS CIRCULANS
STARCH CONVERSION
ULTRAFILTRATION MEMBRANE REACTOR
title_short Continuous production of cyclodextrins in an ultrafiltration membrane reactor, catalyzed by Cyclodextrin glycosyltransferase from Bacillus circulans DF 9R
title_full Continuous production of cyclodextrins in an ultrafiltration membrane reactor, catalyzed by Cyclodextrin glycosyltransferase from Bacillus circulans DF 9R
title_fullStr Continuous production of cyclodextrins in an ultrafiltration membrane reactor, catalyzed by Cyclodextrin glycosyltransferase from Bacillus circulans DF 9R
title_full_unstemmed Continuous production of cyclodextrins in an ultrafiltration membrane reactor, catalyzed by Cyclodextrin glycosyltransferase from Bacillus circulans DF 9R
title_sort Continuous production of cyclodextrins in an ultrafiltration membrane reactor, catalyzed by Cyclodextrin glycosyltransferase from Bacillus circulans DF 9R
dc.creator.none.fl_str_mv Rodríguez Gastón, Jorgelina Andrea
Costa, Hernán
Ferrarotti, Susana Alicia
author Rodríguez Gastón, Jorgelina Andrea
author_facet Rodríguez Gastón, Jorgelina Andrea
Costa, Hernán
Ferrarotti, Susana Alicia
author_role author
author2 Costa, Hernán
Ferrarotti, Susana Alicia
author2_role author
author
dc.subject.none.fl_str_mv CYCLODEXTRINS
CYCLODEXTRIN GLYCOSYLTRANSFERASE
BACILLUS CIRCULANS
STARCH CONVERSION
ULTRAFILTRATION MEMBRANE REACTOR
topic CYCLODEXTRINS
CYCLODEXTRIN GLYCOSYLTRANSFERASE
BACILLUS CIRCULANS
STARCH CONVERSION
ULTRAFILTRATION MEMBRANE REACTOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Cyclodextrins (CDs) are cyclic oligosaccharides of wide industrial application, whose synthesis is catalyzed by Cyclodextrin glycosyltransferase (CGTase) from starch. Here, CDs were produced using CGTase from Bacillus circulans DF 9R in continuous process and an ultrafiltration membrane reactor. The batch process was conducted as a control. This method allowed increasing the yield from 40% to 55.6% and the productivity from 26.1 to 99.5 mg of CD per unit of enzyme. The method also allowed obtaining a high purity product. The flow rate remained at 50% of its initial value after 24 hours of process, improving the results described in the literature for starch hydrolysis processes. CGTase remained active throughout the process, which could be explained by the protective effect of the substrate and reaction products on CGTase stability. In addition, batch processes were developed using starches from different sources. We concluded that any of the starches studied could be used as substrate for CD production with similar yields and product specificity.
Fil: Rodríguez Gastón, Jorgelina Andrea. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; Argentina
Fil: Costa, Hernán. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ferrarotti, Susana Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; Argentina
description Cyclodextrins (CDs) are cyclic oligosaccharides of wide industrial application, whose synthesis is catalyzed by Cyclodextrin glycosyltransferase (CGTase) from starch. Here, CDs were produced using CGTase from Bacillus circulans DF 9R in continuous process and an ultrafiltration membrane reactor. The batch process was conducted as a control. This method allowed increasing the yield from 40% to 55.6% and the productivity from 26.1 to 99.5 mg of CD per unit of enzyme. The method also allowed obtaining a high purity product. The flow rate remained at 50% of its initial value after 24 hours of process, improving the results described in the literature for starch hydrolysis processes. CGTase remained active throughout the process, which could be explained by the protective effect of the substrate and reaction products on CGTase stability. In addition, batch processes were developed using starches from different sources. We concluded that any of the starches studied could be used as substrate for CD production with similar yields and product specificity.
publishDate 2015
dc.date.none.fl_str_mv 2015-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/109312
Rodríguez Gastón, Jorgelina Andrea; Costa, Hernán; Ferrarotti, Susana Alicia; Continuous production of cyclodextrins in an ultrafiltration membrane reactor, catalyzed by Cyclodextrin glycosyltransferase from Bacillus circulans DF 9R; American Chemical Society; Biotechnology Progress; 31; 3; 1-2015; 695-699
8756-7938
CONICET Digital
CONICET
url http://hdl.handle.net/11336/109312
identifier_str_mv Rodríguez Gastón, Jorgelina Andrea; Costa, Hernán; Ferrarotti, Susana Alicia; Continuous production of cyclodextrins in an ultrafiltration membrane reactor, catalyzed by Cyclodextrin glycosyltransferase from Bacillus circulans DF 9R; American Chemical Society; Biotechnology Progress; 31; 3; 1-2015; 695-699
8756-7938
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/BTPR.2053
info:eu-repo/semantics/altIdentifier/url/https://aiche.onlinelibrary.wiley.com/doi/abs/10.1002/btpr.2053
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.260194

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