Identification and characterization of the ecdysteroid UDP-glycosyltransferase gene of <i>Epinotia aporema</i> granulovirus

Autores
Manzán, María Alejandra; Lozano, Mario Enrique; Sciocco de Cap, Alicia; Ghiringhelli, Pablo Daniel; Romanowski, Víctor
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The ecdysteroid UDP-glycosyltransferase (egt) gene of Epinotia aporema granulovirus (EpapGV) was cloned sequenced and its biological activity was assessed. It encodes a protein of 446 amino acids. Direct evidence that the cloned gene encodes an active EGT protein was obtained by transient expression assays in insect cells. The upstream untranslated region of the egt gene exhibits several consensus early promoter elements. Accordingly, the gene is expressed early upon infection of Epinotia aporema larvae and the EGT activity remains high until later times post infection. Sequence analyses indicate the presence of clusters of amino acid residues conserved among all the baculoviral EGTs, although their relation with proper protein folding, ligand binding and catalytic activity remain to be assessed. Phylogenetic trees consistently cluster the granulovirus EGTs separating them clearly from the nucleopolyhedroviruses.
Instituto de Biotecnología y Biología Molecular
Materia
Biología
baculoviruses
ecdysone glycosyltransferase (egt)
EpapGV
gene function
granuloviruses
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/136119

id SEDICI_99c726f5fc6421d8a63f408f02aaa8e0
oai_identifier_str oai:sedici.unlp.edu.ar:10915/136119
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Identification and characterization of the ecdysteroid UDP-glycosyltransferase gene of <i>Epinotia aporema</i> granulovirusManzán, María AlejandraLozano, Mario EnriqueSciocco de Cap, AliciaGhiringhelli, Pablo DanielRomanowski, VíctorBiologíabaculovirusesecdysone glycosyltransferase (egt)EpapGVgene functiongranulovirusesThe ecdysteroid UDP-glycosyltransferase (egt) gene of <i>Epinotia aporema</i> granulovirus (EpapGV) was cloned sequenced and its biological activity was assessed. It encodes a protein of 446 amino acids. Direct evidence that the cloned gene encodes an active EGT protein was obtained by transient expression assays in insect cells. The upstream untranslated region of the egt gene exhibits several consensus early promoter elements. Accordingly, the gene is expressed early upon infection of <i>Epinotia aporema</i> larvae and the EGT activity remains high until later times post infection. Sequence analyses indicate the presence of clusters of amino acid residues conserved among all the baculoviral EGTs, although their relation with proper protein folding, ligand binding and catalytic activity remain to be assessed. Phylogenetic trees consistently cluster the granulovirus EGTs separating them clearly from the nucleopolyhedroviruses.Instituto de Biotecnología y Biología Molecular2002-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf119-130http://sedici.unlp.edu.ar/handle/10915/136119enginfo:eu-repo/semantics/altIdentifier/issn/0920-8569info:eu-repo/semantics/altIdentifier/issn/1572-994Xinfo:eu-repo/semantics/altIdentifier/doi/10.1023/a:1014564331383info:eu-repo/semantics/altIdentifier/pmid/12018702info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:31:58Zoai:sedici.unlp.edu.ar:10915/136119Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:31:58.327SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Identification and characterization of the ecdysteroid UDP-glycosyltransferase gene of <i>Epinotia aporema</i> granulovirus
title Identification and characterization of the ecdysteroid UDP-glycosyltransferase gene of <i>Epinotia aporema</i> granulovirus
spellingShingle Identification and characterization of the ecdysteroid UDP-glycosyltransferase gene of <i>Epinotia aporema</i> granulovirus
Manzán, María Alejandra
Biología
baculoviruses
ecdysone glycosyltransferase (egt)
EpapGV
gene function
granuloviruses
title_short Identification and characterization of the ecdysteroid UDP-glycosyltransferase gene of <i>Epinotia aporema</i> granulovirus
title_full Identification and characterization of the ecdysteroid UDP-glycosyltransferase gene of <i>Epinotia aporema</i> granulovirus
title_fullStr Identification and characterization of the ecdysteroid UDP-glycosyltransferase gene of <i>Epinotia aporema</i> granulovirus
title_full_unstemmed Identification and characterization of the ecdysteroid UDP-glycosyltransferase gene of <i>Epinotia aporema</i> granulovirus
title_sort Identification and characterization of the ecdysteroid UDP-glycosyltransferase gene of <i>Epinotia aporema</i> granulovirus
dc.creator.none.fl_str_mv Manzán, María Alejandra
Lozano, Mario Enrique
Sciocco de Cap, Alicia
Ghiringhelli, Pablo Daniel
Romanowski, Víctor
author Manzán, María Alejandra
author_facet Manzán, María Alejandra
Lozano, Mario Enrique
Sciocco de Cap, Alicia
Ghiringhelli, Pablo Daniel
Romanowski, Víctor
author_role author
author2 Lozano, Mario Enrique
Sciocco de Cap, Alicia
Ghiringhelli, Pablo Daniel
Romanowski, Víctor
author2_role author
author
author
author
dc.subject.none.fl_str_mv Biología
baculoviruses
ecdysone glycosyltransferase (egt)
EpapGV
gene function
granuloviruses
topic Biología
baculoviruses
ecdysone glycosyltransferase (egt)
EpapGV
gene function
granuloviruses
dc.description.none.fl_txt_mv The ecdysteroid UDP-glycosyltransferase (egt) gene of <i>Epinotia aporema</i> granulovirus (EpapGV) was cloned sequenced and its biological activity was assessed. It encodes a protein of 446 amino acids. Direct evidence that the cloned gene encodes an active EGT protein was obtained by transient expression assays in insect cells. The upstream untranslated region of the egt gene exhibits several consensus early promoter elements. Accordingly, the gene is expressed early upon infection of <i>Epinotia aporema</i> larvae and the EGT activity remains high until later times post infection. Sequence analyses indicate the presence of clusters of amino acid residues conserved among all the baculoviral EGTs, although their relation with proper protein folding, ligand binding and catalytic activity remain to be assessed. Phylogenetic trees consistently cluster the granulovirus EGTs separating them clearly from the nucleopolyhedroviruses.
Instituto de Biotecnología y Biología Molecular
description The ecdysteroid UDP-glycosyltransferase (egt) gene of <i>Epinotia aporema</i> granulovirus (EpapGV) was cloned sequenced and its biological activity was assessed. It encodes a protein of 446 amino acids. Direct evidence that the cloned gene encodes an active EGT protein was obtained by transient expression assays in insect cells. The upstream untranslated region of the egt gene exhibits several consensus early promoter elements. Accordingly, the gene is expressed early upon infection of <i>Epinotia aporema</i> larvae and the EGT activity remains high until later times post infection. Sequence analyses indicate the presence of clusters of amino acid residues conserved among all the baculoviral EGTs, although their relation with proper protein folding, ligand binding and catalytic activity remain to be assessed. Phylogenetic trees consistently cluster the granulovirus EGTs separating them clearly from the nucleopolyhedroviruses.
publishDate 2002
dc.date.none.fl_str_mv 2002-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/136119
url http://sedici.unlp.edu.ar/handle/10915/136119
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0920-8569
info:eu-repo/semantics/altIdentifier/issn/1572-994X
info:eu-repo/semantics/altIdentifier/doi/10.1023/a:1014564331383
info:eu-repo/semantics/altIdentifier/pmid/12018702
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
119-130
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
_version_ 1844616199499939840
score 13.070432