Identification and characterization of the ecdysteroid UDP-glycosyltransferase gene of <i>Epinotia aporema</i> granulovirus
- Autores
- Manzán, María Alejandra; Lozano, Mario Enrique; Sciocco de Cap, Alicia; Ghiringhelli, Pablo Daniel; Romanowski, Víctor
- Año de publicación
- 2002
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The ecdysteroid UDP-glycosyltransferase (egt) gene of Epinotia aporema granulovirus (EpapGV) was cloned sequenced and its biological activity was assessed. It encodes a protein of 446 amino acids. Direct evidence that the cloned gene encodes an active EGT protein was obtained by transient expression assays in insect cells. The upstream untranslated region of the egt gene exhibits several consensus early promoter elements. Accordingly, the gene is expressed early upon infection of Epinotia aporema larvae and the EGT activity remains high until later times post infection. Sequence analyses indicate the presence of clusters of amino acid residues conserved among all the baculoviral EGTs, although their relation with proper protein folding, ligand binding and catalytic activity remain to be assessed. Phylogenetic trees consistently cluster the granulovirus EGTs separating them clearly from the nucleopolyhedroviruses.
Instituto de Biotecnología y Biología Molecular - Materia
-
Biología
baculoviruses
ecdysone glycosyltransferase (egt)
EpapGV
gene function
granuloviruses - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/136119
Ver los metadatos del registro completo
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Identification and characterization of the ecdysteroid UDP-glycosyltransferase gene of <i>Epinotia aporema</i> granulovirusManzán, María AlejandraLozano, Mario EnriqueSciocco de Cap, AliciaGhiringhelli, Pablo DanielRomanowski, VíctorBiologíabaculovirusesecdysone glycosyltransferase (egt)EpapGVgene functiongranulovirusesThe ecdysteroid UDP-glycosyltransferase (egt) gene of <i>Epinotia aporema</i> granulovirus (EpapGV) was cloned sequenced and its biological activity was assessed. It encodes a protein of 446 amino acids. Direct evidence that the cloned gene encodes an active EGT protein was obtained by transient expression assays in insect cells. The upstream untranslated region of the egt gene exhibits several consensus early promoter elements. Accordingly, the gene is expressed early upon infection of <i>Epinotia aporema</i> larvae and the EGT activity remains high until later times post infection. Sequence analyses indicate the presence of clusters of amino acid residues conserved among all the baculoviral EGTs, although their relation with proper protein folding, ligand binding and catalytic activity remain to be assessed. Phylogenetic trees consistently cluster the granulovirus EGTs separating them clearly from the nucleopolyhedroviruses.Instituto de Biotecnología y Biología Molecular2002-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf119-130http://sedici.unlp.edu.ar/handle/10915/136119enginfo:eu-repo/semantics/altIdentifier/issn/0920-8569info:eu-repo/semantics/altIdentifier/issn/1572-994Xinfo:eu-repo/semantics/altIdentifier/doi/10.1023/a:1014564331383info:eu-repo/semantics/altIdentifier/pmid/12018702info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:31:58Zoai:sedici.unlp.edu.ar:10915/136119Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:31:58.327SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Identification and characterization of the ecdysteroid UDP-glycosyltransferase gene of <i>Epinotia aporema</i> granulovirus |
title |
Identification and characterization of the ecdysteroid UDP-glycosyltransferase gene of <i>Epinotia aporema</i> granulovirus |
spellingShingle |
Identification and characterization of the ecdysteroid UDP-glycosyltransferase gene of <i>Epinotia aporema</i> granulovirus Manzán, María Alejandra Biología baculoviruses ecdysone glycosyltransferase (egt) EpapGV gene function granuloviruses |
title_short |
Identification and characterization of the ecdysteroid UDP-glycosyltransferase gene of <i>Epinotia aporema</i> granulovirus |
title_full |
Identification and characterization of the ecdysteroid UDP-glycosyltransferase gene of <i>Epinotia aporema</i> granulovirus |
title_fullStr |
Identification and characterization of the ecdysteroid UDP-glycosyltransferase gene of <i>Epinotia aporema</i> granulovirus |
title_full_unstemmed |
Identification and characterization of the ecdysteroid UDP-glycosyltransferase gene of <i>Epinotia aporema</i> granulovirus |
title_sort |
Identification and characterization of the ecdysteroid UDP-glycosyltransferase gene of <i>Epinotia aporema</i> granulovirus |
dc.creator.none.fl_str_mv |
Manzán, María Alejandra Lozano, Mario Enrique Sciocco de Cap, Alicia Ghiringhelli, Pablo Daniel Romanowski, Víctor |
author |
Manzán, María Alejandra |
author_facet |
Manzán, María Alejandra Lozano, Mario Enrique Sciocco de Cap, Alicia Ghiringhelli, Pablo Daniel Romanowski, Víctor |
author_role |
author |
author2 |
Lozano, Mario Enrique Sciocco de Cap, Alicia Ghiringhelli, Pablo Daniel Romanowski, Víctor |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Biología baculoviruses ecdysone glycosyltransferase (egt) EpapGV gene function granuloviruses |
topic |
Biología baculoviruses ecdysone glycosyltransferase (egt) EpapGV gene function granuloviruses |
dc.description.none.fl_txt_mv |
The ecdysteroid UDP-glycosyltransferase (egt) gene of <i>Epinotia aporema</i> granulovirus (EpapGV) was cloned sequenced and its biological activity was assessed. It encodes a protein of 446 amino acids. Direct evidence that the cloned gene encodes an active EGT protein was obtained by transient expression assays in insect cells. The upstream untranslated region of the egt gene exhibits several consensus early promoter elements. Accordingly, the gene is expressed early upon infection of <i>Epinotia aporema</i> larvae and the EGT activity remains high until later times post infection. Sequence analyses indicate the presence of clusters of amino acid residues conserved among all the baculoviral EGTs, although their relation with proper protein folding, ligand binding and catalytic activity remain to be assessed. Phylogenetic trees consistently cluster the granulovirus EGTs separating them clearly from the nucleopolyhedroviruses. Instituto de Biotecnología y Biología Molecular |
description |
The ecdysteroid UDP-glycosyltransferase (egt) gene of <i>Epinotia aporema</i> granulovirus (EpapGV) was cloned sequenced and its biological activity was assessed. It encodes a protein of 446 amino acids. Direct evidence that the cloned gene encodes an active EGT protein was obtained by transient expression assays in insect cells. The upstream untranslated region of the egt gene exhibits several consensus early promoter elements. Accordingly, the gene is expressed early upon infection of <i>Epinotia aporema</i> larvae and the EGT activity remains high until later times post infection. Sequence analyses indicate the presence of clusters of amino acid residues conserved among all the baculoviral EGTs, although their relation with proper protein folding, ligand binding and catalytic activity remain to be assessed. Phylogenetic trees consistently cluster the granulovirus EGTs separating them clearly from the nucleopolyhedroviruses. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002-03 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/136119 |
url |
http://sedici.unlp.edu.ar/handle/10915/136119 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/issn/0920-8569 info:eu-repo/semantics/altIdentifier/issn/1572-994X info:eu-repo/semantics/altIdentifier/doi/10.1023/a:1014564331383 info:eu-repo/semantics/altIdentifier/pmid/12018702 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
dc.format.none.fl_str_mv |
application/pdf 119-130 |
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SEDICI (UNLP) - Universidad Nacional de La Plata |
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