Cyclodextrin production by cyclodextrin glycosyltransferase from Bacillus circulans DF 9R

Autores
Szerman, Natalia; Schroh, Ignacio; Rossi, Ana Lía; Rosso, Adriana Mabel; Krymkiewicz, Norberto; Ferrarotti, Susana Alicia
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cyclodextrins (CD) are cyclic oligosaccharides with multiple applications in the food, pharmaceutical, cosmetic, agricultural and chemical industries. In this work, the conditions used to produce CD with cyclodextrin glycosyltransferase from Bacillus circulans DF 9R were optimized using experimental designs. The developed method allowed the partial purification and concentration of the enzyme from the cultural broth and, subsequently, the CD production, using the same cassava starch as enzyme adsorbent and as substrate. Heat-treatment of raw starch at 70 C for 15 min in the presence of adsorbed cyclodextrin glycosyltransferase allowed the starch liquefaction without enzyme inactivation. The optimum conditions for CD production were: 5% (w/v) cassava starch, 15 U of enzyme per gram of substrate, reaction temperature of 56 C and pH 6.4. After 4 h, the proportion of starch converted to CD reached 66% (w/w) and the weight ratio of a-CD:b-CD:c-CD was 1.00:0.70:0.16.
Fil: Szerman, Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina.
Fil: Schroh, Ignacio. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina.
Fil: Rossi, Ana Lía. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina.
Fil: Rosso, Adriana Mabel. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina.
Fil: Krymkiewicz, Norberto. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina.
Fil: Ferrarotti, Susana Alicia. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina.
Fuente
Bioresource Technology 98 (15) : 2886–2891 (November 2007).
Materia
Cyclodextrins
Ciclodextrinas
Bacillus circulans
Cyclodextrins Production
Cyclodextrin glycosyltransferase
Producción de ciclodextrinas
Bacillus circulans DF 9R
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/5951
Acceder
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oai_identifier_str oai:localhost:20.500.12123/5951
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Cyclodextrin production by cyclodextrin glycosyltransferase from Bacillus circulans DF 9RSzerman, NataliaSchroh, IgnacioRossi, Ana LíaRosso, Adriana MabelKrymkiewicz, NorbertoFerrarotti, Susana AliciaCyclodextrinsCiclodextrinasBacillus circulansCyclodextrins ProductionCyclodextrin glycosyltransferaseProducción de ciclodextrinasBacillus circulans DF 9RCyclodextrins (CD) are cyclic oligosaccharides with multiple applications in the food, pharmaceutical, cosmetic, agricultural and chemical industries. In this work, the conditions used to produce CD with cyclodextrin glycosyltransferase from Bacillus circulans DF 9R were optimized using experimental designs. The developed method allowed the partial purification and concentration of the enzyme from the cultural broth and, subsequently, the CD production, using the same cassava starch as enzyme adsorbent and as substrate. Heat-treatment of raw starch at 70 C for 15 min in the presence of adsorbed cyclodextrin glycosyltransferase allowed the starch liquefaction without enzyme inactivation. The optimum conditions for CD production were: 5% (w/v) cassava starch, 15 U of enzyme per gram of substrate, reaction temperature of 56 C and pH 6.4. After 4 h, the proportion of starch converted to CD reached 66% (w/w) and the weight ratio of a-CD:b-CD:c-CD was 1.00:0.70:0.16.Fil: Szerman, Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina.Fil: Schroh, Ignacio. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina.Fil: Rossi, Ana Lía. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina.Fil: Rosso, Adriana Mabel. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina.Fil: Krymkiewicz, Norberto. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina.Fil: Ferrarotti, Susana Alicia. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina.Elsevier2019-09-24T11:20:16Z2019-09-24T11:20:16Z2006-09-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S0960852406005281http://hdl.handle.net/20.500.12123/59510960-8524https://doi.org/10.1016/j.biortech.2006.09.056Bioresource Technology 98 (15) : 2886–2891 (November 2007).reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:48:10Zoai:localhost:20.500.12123/5951instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:11.507INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Cyclodextrin production by cyclodextrin glycosyltransferase from Bacillus circulans DF 9R
title Cyclodextrin production by cyclodextrin glycosyltransferase from Bacillus circulans DF 9R
spellingShingle Cyclodextrin production by cyclodextrin glycosyltransferase from Bacillus circulans DF 9R
Szerman, Natalia
Cyclodextrins
Ciclodextrinas
Bacillus circulans
Cyclodextrins Production
Cyclodextrin glycosyltransferase
Producción de ciclodextrinas
Bacillus circulans DF 9R
title_short Cyclodextrin production by cyclodextrin glycosyltransferase from Bacillus circulans DF 9R
title_full Cyclodextrin production by cyclodextrin glycosyltransferase from Bacillus circulans DF 9R
title_fullStr Cyclodextrin production by cyclodextrin glycosyltransferase from Bacillus circulans DF 9R
title_full_unstemmed Cyclodextrin production by cyclodextrin glycosyltransferase from Bacillus circulans DF 9R
title_sort Cyclodextrin production by cyclodextrin glycosyltransferase from Bacillus circulans DF 9R
dc.creator.none.fl_str_mv Szerman, Natalia
Schroh, Ignacio
Rossi, Ana Lía
Rosso, Adriana Mabel
Krymkiewicz, Norberto
Ferrarotti, Susana Alicia
author Szerman, Natalia
author_facet Szerman, Natalia
Schroh, Ignacio
Rossi, Ana Lía
Rosso, Adriana Mabel
Krymkiewicz, Norberto
Ferrarotti, Susana Alicia
author_role author
author2 Schroh, Ignacio
Rossi, Ana Lía
Rosso, Adriana Mabel
Krymkiewicz, Norberto
Ferrarotti, Susana Alicia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Cyclodextrins
Ciclodextrinas
Bacillus circulans
Cyclodextrins Production
Cyclodextrin glycosyltransferase
Producción de ciclodextrinas
Bacillus circulans DF 9R
topic Cyclodextrins
Ciclodextrinas
Bacillus circulans
Cyclodextrins Production
Cyclodextrin glycosyltransferase
Producción de ciclodextrinas
Bacillus circulans DF 9R
dc.description.none.fl_txt_mv Cyclodextrins (CD) are cyclic oligosaccharides with multiple applications in the food, pharmaceutical, cosmetic, agricultural and chemical industries. In this work, the conditions used to produce CD with cyclodextrin glycosyltransferase from Bacillus circulans DF 9R were optimized using experimental designs. The developed method allowed the partial purification and concentration of the enzyme from the cultural broth and, subsequently, the CD production, using the same cassava starch as enzyme adsorbent and as substrate. Heat-treatment of raw starch at 70 C for 15 min in the presence of adsorbed cyclodextrin glycosyltransferase allowed the starch liquefaction without enzyme inactivation. The optimum conditions for CD production were: 5% (w/v) cassava starch, 15 U of enzyme per gram of substrate, reaction temperature of 56 C and pH 6.4. After 4 h, the proportion of starch converted to CD reached 66% (w/w) and the weight ratio of a-CD:b-CD:c-CD was 1.00:0.70:0.16.
Fil: Szerman, Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina.
Fil: Schroh, Ignacio. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina.
Fil: Rossi, Ana Lía. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina.
Fil: Rosso, Adriana Mabel. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina.
Fil: Krymkiewicz, Norberto. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina.
Fil: Ferrarotti, Susana Alicia. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina.
description Cyclodextrins (CD) are cyclic oligosaccharides with multiple applications in the food, pharmaceutical, cosmetic, agricultural and chemical industries. In this work, the conditions used to produce CD with cyclodextrin glycosyltransferase from Bacillus circulans DF 9R were optimized using experimental designs. The developed method allowed the partial purification and concentration of the enzyme from the cultural broth and, subsequently, the CD production, using the same cassava starch as enzyme adsorbent and as substrate. Heat-treatment of raw starch at 70 C for 15 min in the presence of adsorbed cyclodextrin glycosyltransferase allowed the starch liquefaction without enzyme inactivation. The optimum conditions for CD production were: 5% (w/v) cassava starch, 15 U of enzyme per gram of substrate, reaction temperature of 56 C and pH 6.4. After 4 h, the proportion of starch converted to CD reached 66% (w/w) and the weight ratio of a-CD:b-CD:c-CD was 1.00:0.70:0.16.
publishDate 2006
dc.date.none.fl_str_mv 2006-09-30
2019-09-24T11:20:16Z
2019-09-24T11:20:16Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0960852406005281
http://hdl.handle.net/20.500.12123/5951
0960-8524
https://doi.org/10.1016/j.biortech.2006.09.056
url https://www.sciencedirect.com/science/article/pii/S0960852406005281
http://hdl.handle.net/20.500.12123/5951
https://doi.org/10.1016/j.biortech.2006.09.056
identifier_str_mv 0960-8524
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Bioresource Technology 98 (15) : 2886–2891 (November 2007).
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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